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Effect of long-term high-fat feeding on energy balance and liver oxidative activity in rats

Published online by Cambridge University Press:  09 March 2007

Susanna Iossa ,
Lillà Lionetti ,
Maria P. Mollica ,
Raffaella Crescenzo ,
Antonio Barletta  and
Giovanna Liverini
Susanna Iossa
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Lillà Lionetti
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Maria P. Mollica
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Raffaella Crescenzo
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
Antonio Barletta
Affiliation:
Department of General and Environmental Physiology, University of Naples “FEDERICO II”, Naples, Italy
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Abstract

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The purpose of this work was to study the effect of early long-term high-fat feeding on energy balance and liver oxidative activity. To this end, rats aged about 30 d were fed a high-fat or a low-fat diet for 15, 30 or 60 d. Full energy balance and energy partitioning measurements were carried out. In addition, we measured hepatic mitochondrial and peroxisomal oxidative capacities. Serum levels of free triiodothyronine (T3) and leptin were also determined. Rats fed a high-fat diet showed an increase in metabolizable energy intake as well as in energy expenditure, while lipid gain over the whole period was lower than that expected due to a decrease in metabolic efficiency. An increase in serum free T3 levels was also found in rats fed a high-fat diet after 15 and 30 d. Statistically significant correlations between serum leptin levels and body fat mass were found after 15, 30 and 60 d of high-fat feeding. Finally, no variation in hepatic mitochondrial and peroxisomal fatty acid oxidation capacity was found in rats fed a high-fat diet for 15, 30 or 60 d. In conclusion, the results of the present study show that young Wistar rats fed a high-fat diet for up to 60 d are able to counteract, at least in part, obesity development.

Keywords

High-fat dietEnergy balanceHepatic respiration
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 3 , September 2000 , pp. 377 - 385
Copyright
Copyright © The Nutrition Society 2000

References

Allard, M and LeBlanc, J (1988) Effect of cold acclimation, cold exposure, and palatability on postprandial thermogenesis in rats.International Journal of Obesity 12, 169–178.Google ScholarPubMed
American Institute of Nutrition (1977) , Report of the American Institute of Nutrition .ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Brady, PS, Knoeber, CM and Brady, LJ (1986) Hepatic mitochondrial and peroxisomal oxidative capacity in riboflavin deficiency: effect of age, dietary fat and starvation in rats.Journal of Nutrition 116, 1992–1999.CrossRefGoogle ScholarPubMed
Brooks, SPJ, Lampi, BJ, Sarwar, G and Botting, HG (1995) A comparison of methods for determining total body protein.Analytical Biochemistry 226, 26–30.CrossRefGoogle ScholarPubMed
Danforth, E and Burger, AG (1989) The impact of nutrition on thyroid hormone physiology and action.Annual Review of Nutrition 9, 201–227.CrossRefGoogle ScholarPubMed
Da-Wei, G, Yufang, HE, Karas, M and Reitman, M (1997) Uncoupling protein 3 is a mediator of thermogenesis regulated by thyroid hormone, beta3-adrenergic agonist, and leptin.Journal of Biological Chemistry 26, 24129–24132.Google Scholar
De Schepper, J, Zhou, X, De Bock, S, Smitz, J, Louis, O, Hooghe-Peters, E and Vandenplas, Y (1998) Study of serum leptin in cafeteria-diet-overfed rats: influence of diet, insulin and corticosterone.Hormone Research 50, 271–275.CrossRefGoogle ScholarPubMed
Escobar-Morreale, HF, Escobar del Rey, F and Morreale de Escobar, G (1997) Thyroid hormones influence serum leptin concentrations in the rat.Endocrinology 138, 4485–4488.CrossRefGoogle ScholarPubMed
Esteve, M, Rafecas, I, Fernandez-Lopez, JA, Remesar, X and Alemany, M (1992) Fatty acid utilization by young Wistar rats fed a cafeteria diet.Molecular and Cellular Biochemistry 118, 67–74.CrossRefGoogle ScholarPubMed
Fain, JN and Bahouth, SW (1998) Effect of tri-iodothyronine on leptin release and leptin mRNA accumulation in rat adipose tissue.Biochemical Journal 332, 361–366.CrossRefGoogle ScholarPubMed
Fain, JN, Coronel, EC, Beauchamp, MJ and Bahouth, SW (1997) Expression of leptin and β3-adrenergic receptors in rat adipose tissue in altered thyroid states.Biochemical Journal 322, 145–150.CrossRefGoogle Scholar
Folch, J, Lees, M and Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues.Journal of Biological Chemistry 226, 497–510.CrossRefGoogle ScholarPubMed
Freake, HC and Oppenheimer, JH (1995) Thermogenesis and thyroid function.Annual Review of Nutrition 15, 263–291.CrossRefGoogle ScholarPubMed
Friedman, JM and Halaas, JL (1998) Leptin and the regulation of body weight in mammals.Nature 395, 763–770.CrossRefGoogle ScholarPubMed
Guzman, M and Geelen, MJH (1993) Regulation of fatty acid oxidation in mammalian liver.Biochimica et Biophysica Acta 1167, 227–241.CrossRefGoogle ScholarPubMed
Hansen, PA, Han, DH, Nolte, LA, Chen, M and Holloszy, JO (1997) DHEA protects against visceral obesity and muscle insulin resistance in rats fed a high-fat diet.American Journal of Physiology 273, R1704–R1708.Google ScholarPubMed
Inestrosa, NC, Bronfman, M and Leighton, F (1979) Detection of peroxisomal fatty acyl-Coenzyme A oxidase activity.Biochemical Journal 182, 779–788.CrossRefGoogle ScholarPubMed
Iossa, S, Mollica, MP, Lionetti, L, Barletta, A and Liverini, G (1995) Hepatic mitochondrial respiration and transfer of reducing equivalents in rats fed an energy dense diet.International Journal of Obesity 19, 539–543.Google Scholar
Iossa, S, Mollica, MP, Lionetti, L, Barletta, A and Liverini, G (1997) Effect of high-fat diet on energy balance and thermic effect of food in hypothyroid rats.European Journal of Endocrinology 136, 309–315.CrossRefGoogle ScholarPubMed
Iossa, S, Mollica, MP, Lionetti, L, Barletta, A and Liverini, G (1997) Energy balance and liver respiratory activity in rats fed on an energy-dense diet.British Journal of Nutrition 77, 99–105.CrossRefGoogle Scholar
Iossa, S, Lionetti, L, Mollica, MP, Barletta, A and Liverini, G (1999) Fat balance and hepatic mitochondrial function in response to fat feeding in mature rats.International Journal of Obesity 23, 1–7.CrossRefGoogle ScholarPubMed
Ishii, H, Fukumori, N, Horie, S and Suga, T (1980) Effects of fat content in the diet on hepatic peroxisomes of the rat.Biochimica et Biophysica Acta 617, 1–11.CrossRefGoogle ScholarPubMed
Larkin, S, Mull, E, Miao, W, Pitter, R, Albrandt, K, Moore, C, Young, A, Denaro, M and Beaumont, K (1997) Regulation of the third member of the uncoupling protein family, UCP3, by cold and thyroid hormone.Biochemical and Biophysical Research Communications 240, 222–227.CrossRefGoogle ScholarPubMed
Lawson, N, Jennings, RJ, Pollard, AD, Sturton, RG, Ralph, SJ, Marsden, CA, Fears, R and Brindley, DN (1981) Effects of chronic modification of dietary fat and carbohydrate in rats.Biochemical Journal 200, 265–273.CrossRefGoogle ScholarPubMed
Levin, BE, Finnegan, MB, Triscari, J and Sullivan, AC (1985) Brown adipose tissue and metabolic features of chronic diet-induced obesity.American Journal of Physiology 248, R717–R723.Google ScholarPubMed
Levin, BE, Triscari, J and Sullivan, AC (1983) Relationship between sympathetic activity and diet-induced obesity in two rat strains.American Journal of Physiology 245, R367–R371.Google ScholarPubMed
Levin, BE, Triscari, J and Sullivan, AC (1986) Metabolic features of diet-induced obesity without hyperphagia in young rats.American Journal of Physiology 251, R433–R440.Google ScholarPubMed
Lionetti, L, Iossa, S, Brand, MD and Liverini, G (1996) Relationship between membrane potential and respiration rate in isolated liver mitochondria from rats fed an energy dense diet.Molecular and Cellular Biochemistry 158, 133–138.Google ScholarPubMed
Liverini, G, Iossa, S and Barletta, A (1994) Hepatic mitochondrial respiratory capacity in hyperphagic rats.Nutrition Research 14, 1671–1682.CrossRefGoogle Scholar
Liverini, G, Iossa, S, Lionetti, L, Mollica, MP and Barletta, A (1995) Sympathetically-mediated thermogenic response to food in rats.International Journal of Obesity 19, 87–91.Google ScholarPubMed
Liverini, G, Iossa, S, Lionetti, L, Mollica, MP and Barletta, A (1996) Hepatic fatty acid-supported respiration in rats fed an energy dense diet.Cell Biochemistry and Function 14, 283–289.CrossRefGoogle ScholarPubMed
Masaki, T, Yoshimatsu, H, Kakuma, T, Hidaka, S, Kurokawa, M and Sakata, T (1997) Enhanced expression of uncoupling protein 2 gene in rat white adipose tissue and skeletal muscle following chronic treatment with thyroid hormone.FEBS Letters 418, 323–326.CrossRefGoogle ScholarPubMed
Mollica, MP, Iossa, S, Liverini, G and Soboll, S (1998) Steady state changes in mitochondrial electrical potential and proton gradient in perfused liver from rats fed a high-fat diet.Molecular and Cellular Biochemistry 178, 213–217.CrossRefGoogle ScholarPubMed
Naim, M, Brand, JG, Kare, MR and Carpenter, RG (1985) Energy intake, weight gain, and fat deposition in rats fed nutritionally controlled diet in a multichoice ("cafeteria") design.Journal of Nutrition 115, 1447–1485.CrossRefGoogle Scholar
Oppenheimer, JH, Schwartz, HL, Lane, JT and Thompson, MP (1991) Functional relationship of thyroid hormone-induced lipogenesis, lipolysis, and thermogenesis in the rat.Journal of Clinical Investigation 87, 125–132.CrossRefGoogle ScholarPubMed
Papamandjaris, AA, MacDougall DE and Jones, PJH (1998) Medium chain fatty acid metabolism and energy expenditure: obesity treatment implications.Life Sciences 62, 1203–1215.CrossRefGoogle ScholarPubMed
Pullar, JD and Webster, AJF (1977) The energy cost of fat and protein deposition in the rat.British Journal of Nutrition 37, 355–363.CrossRefGoogle ScholarPubMed
Ramirez, I and Friedman, MI (1990) Dietary hyperphagia in rats: role of fat, carbohydrate, and energy content.Physiology and Behaviour 47, 1157–1163.CrossRefGoogle ScholarPubMed
Reed, DR, Tordoff, MG and Friedman, MI (1991) Enhanced acceptance and metabolism of fats by rats fed a high-fat diet.American Journal of Physiology 261, R1084–R1088.Google ScholarPubMed
Rothwell, NJ and Stock, MJ (1979) A role for brown adipose tissue in diet-induced thermogenesis.Nature 281, 31–35.CrossRefGoogle ScholarPubMed
Rothwell, NJ, Stock, MJ and Warwick, BP (1985) Energy balance and brown fat activity in rats fed cafeteria diets or high-fat, semisynthetic diets at several levels of intake.Metabolism 34, 474–480.CrossRefGoogle ScholarPubMed
Schultz, H (1991) Beta oxidation of fatty acids.Biochimica et Biophysica Acta 1081, 109–120.CrossRefGoogle Scholar
Veerkamp, JH and Zevenberger, JL (1986) Effect of dietary fat on total and peroxisomal fatty acid oxidation in rat tissues.Biochimica et Biophysica Acta 878, 102–109.CrossRefGoogle ScholarPubMed
Widdowson, PS, Upton, R, Buckingham, R, Arch, J and Williams, G (1997) Inhibition of food response to intracerebroventricular injection of leptin is attenuated in rats with diet-induced obesity.Diabetes 46, 1782–1785.CrossRefGoogle ScholarPubMed