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The effect of Intralipid® infusion on the human leucocyte sodium-pump in vivo

Published online by Cambridge University Press:  09 March 2007

L. L. Ng  and
T. D. R. Hockaday
L. L. Ng
Affiliation:
Sheikh Rashid Diabetes Unit, Radcliffe Infirmary, Oxford OX2 6HE
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Abstract

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1. The effect of unsaturated long-chain non-esterified fatty acids (NEFA) on the human leucocyte sodium-pump was studied in vivo.

2. Plasma NEFA level was raised acutely from 0·28 (sd 0·10) to 2·54 (sd 0·48) mmol/l by infusion of ‘Intralipid 20%’ (trademark) at 90 ml/h with heparin, and the human leucocyte 22Na efflux rate constants were studied in eight normal weight males.

3. After 3 h, there was a significant lowering of the total (from 3·97 (sd 0·92) to 3·10 (sd 0·71)/h; P < 0·01) and ouabain-sensitive 22Na efflux rate constants (from 2·89 (sd 0·55) to 2·37 (sd 0·62)/h; P < 0·02). Ouabain-insensitive efflux rate constants showed a tendency to fall (from 1·08 (sd 0·51) to 0·73 (sd 0·23)/h). Leucocyte potassium content remained unchanged, but sodium content rose from 31 (sd 12) to 38 (sd 18) mmol/kg dry weight (P < 0·05). Total, ouabain-insensitive and ouabain-sensitive efflux rates did not change significantly during the Intralipid-heparin infusion.

4. Plasma insulin levels rose gradually throughout the 3 h infusion period.

5. In conclusion, NEFA, when raised to pathological levels, can inhibit the leucocyte Na-pump in vivo even in the presence of physiological levels of serum albumin, and may increase insulin secretion.

Type
Clinical and Human Nutrition Papers: Studies in Man
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 49 - 55
Copyright
Copyright © The Nutrition Society 1988

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