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Effect of composition of ruminally-infused short-chain fatty acids on net fluxes of nutrients across portal-drained viscera in underfed ewes

Published online by Cambridge University Press:  09 March 2007

Pierre Nozière ,
Cécile Martin ,
Didier Rémond ,
Niels B. Kristensen ,
Richard Bernard  and
Michel Doreau
Pierre Nozière*
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Cécile Martin
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Didier Rémond
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Niels B. Kristensen
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Box 50, DK-8830 Tjele, Denmark
Richard Bernard
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
Michel Doreau
Affiliation:
Unité de Recherches sur les Herbivores, INRA Theix, 63122 Saint-Genès-Champanelle, France
*
*Corresponding author: Dr Pierre Nozière, fax +33 4 73 62 45 19, email noziere@clermont.inra.fr
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Abstract

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Four ewes, each fitted with a rumen cannula and with catheters in the mesenteric artery and portal and mesenteric veins, received continuous intrarumen infusions of water or of short-chain fatty acids (SCFA). SCFA infusions were isoenergetic (83 kJ/h) and provided rumen molar proportions (acetate : propionate : butyrate) of 70 : 20 : 10, 50 : 40 : 10 or 50 : 20 : 30. The rumen SCFA production rate with the basal diet was 90·0, 23·1 and 8·8 mmol/h for acetate, propionate and butyrate respectively. Portal net fluxes indicated that 74, 67 and 22–30 % of infused acetate, propionate and butyrate respectively, reached the portal vein. Portal net release of β-hydroxybutyrate increased with SCFA infusions, irrespective of the amount of butyrate infused. Portal net release of lactate decreased with high-butyrate infusion. Portal net uptake of glucose increased with the SCFA infusions. In ewes infused with water, a portal net uptake of total amino acids (AA) was observed. SCFA infusions decreased the uptake of nonessential AA (glutamate, glycine, but not glutamine) and increased the net release of tyrosine and essential AA (isoleucine, leucine). Portal net fluxes of AA were similar with both high-acetate and high-propionate infusions. Lower net uptake of glutamine and net release of most essential AA and some nonessential AA were observed with the high-butyrate infusion. Energetic summation of portal net release was not significantly different between the three SCFA infusions, although it tended to be lower with high-butyrate infusion. This may be related to the higher trophic effect of butyrate on the digestive mucosa.

Keywords

SheepShort-chain fatty acidsPortal fluxesAmino acids
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 5 , May 2000 , pp. 521 - 531
Copyright
Copyright © The Nutrition Society 2000

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