Hostname: page-component-848d4c4894-8bljj Total loading time: 0 Render date: 2024-07-03T08:02:52.905Z Has data issue: false hasContentIssue false
  • English
  • Français

Dynamics of conjunctival impression cytologic changes after vitamin A supplementation

Published online by Cambridge University Press:  09 March 2007

Swapan Chowdhury ,
N. K. Ganguly ,
Minni Verma ,
Rajesh Kumar ,
Lata Kumar  and
B. N. S. Walia
Swapan Chowdhury
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
N. K. Ganguly
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Minni Verma
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Rajesh Kumar
Affiliation:
Department of Community Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Lata Kumar
Affiliation:
Departments of Paediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
B. N. S. Walia
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

To investigate the chronological changes in conjunctival epithelium after supplementation with a massive oral dose of vitamin A, conjunctival impression cytology (CIC) with transfer was carried out repeatedly among 200 children aged 6–120 months in a randomized, double-blind, placebo-controlled study in Chandigarh (India). Significant conversion to normal CIC started 71–80 d after vitamin A supplementation and by 101–110d conversion had taken place in all children. Compared with the placebo group, plasma retinol concentration at 100d post-supplement was found to be significantly higher in the vitamin A-supplemented group P = 0·04. This study demonstrates that CIC responds to a massive oral dose of vitamin A 3–4 months after supplementation. These findings should guide future studies and evaluations in which CIC is used to assess response to vitamin A interventions.

Keywords

Conjunctival impression cytologyVitamin ARetinol
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 863 - 869
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Amedee-Manesme, O., Luzeau, R., Wittepen, J. R., Hanck, A. & Sommer, A. (1988). Impression cytology detects subclinical vitamin A deficiency. American Journal of Clinical Nutrition 47, 875–888.CrossRefGoogle ScholarPubMed
Bourke, G. J., Daly, L. E. & McGiluray, J. (1985). Interpretation and Uses of Medical Statistics, 3rd ed., pp. 122–124. London: Blackwell Scientific Publications.Google Scholar
Carlier, C., Etchepare, M., Ceccon, J. F., Mourey, M. S. & Amedee-Manesme, O. (1992). Efficacy of massive oral doses of retinyl palmitate and mango (Mangifera indica L.) consumption to correct an existing vitamin A deficiency in Senegalese children. British Journal of Nutrition 68, 529–540.CrossRefGoogle ScholarPubMed
Luzeau, R., Carlier, C., Ellrodt, A. & Amedee-Manesme, O. (1988). Impression cytology with transfer. An easy method for detection of vitamin A deficiency. International Journal for Vitamin and Nutrition Research 58, 166–170.Google ScholarPubMed
Natadisastra, G., Wittpenn, J. R., Muhilal, , West, K., Mele, L. & Sommer, A. (1988). Impression cytology: a practical index of vitamin A status. American Journal of Clinical Nutrition 48, 695–701.CrossRefGoogle ScholarPubMed
Natadisastra, G., Wittpenn, J., West, K., Muhilal, P. & Sommer, A. (1987). Impression cytology for detection of vitamin A deficiency. Archives of Ophthalmology 105, 1224–1228.CrossRefGoogle ScholarPubMed
Olson, J. A. (1984). Serum levels of vitamin A and carotenoids as reflectors of nutritional status. Journal of the National Cancer Institute 73, 1439–1444.Google ScholarPubMed
Thurnham, D. I., Smith, E. & Flora, P. S. (1988). Concurrent liquid chromatographic assay of retinol, α-tocopherol, β-carotene, α-carotene, lycopene and β-cryptoxanthin in plasma with tocopherol acetate as internal standard. Clinical Chemistry 34, 377–381.CrossRefGoogle ScholarPubMed
Usha, N., Sankarnayanan, A., Walia, B. N. S. & Ganguly, N. K. (1990). Early detection of vitamin A deficiency in children with persistent diarrhoea (letter). Lancet 335, 422.CrossRefGoogle Scholar
West, K. P. & Sommer, A. (1987). Periodic, Large Oral Doses of Vitamin A for the Prevention of Vitamin A Deficiency and Xerophthalmia: A Summary of Experiences. A Report of the International Vitamin A Consultative Group. Washington, DC: The Nutrition Foundation.Google Scholar