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Dynamics of conjunctival impression cytologic changes after vitamin A supplementation

Published online by Cambridge University Press:  09 March 2007

Swapan Chowdhury ,
N. K. Ganguly ,
Minni Verma ,
Rajesh Kumar ,
Lata Kumar  and
B. N. S. Walia
Swapan Chowdhury
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
N. K. Ganguly
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Minni Verma
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Rajesh Kumar
Affiliation:
Department of Community Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
Lata Kumar
Affiliation:
Departments of Paediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
B. N. S. Walia
Affiliation:
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India
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Abstract

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To investigate the chronological changes in conjunctival epithelium after supplementation with a massive oral dose of vitamin A, conjunctival impression cytology (CIC) with transfer was carried out repeatedly among 200 children aged 6–120 months in a randomized, double-blind, placebo-controlled study in Chandigarh (India). Significant conversion to normal CIC started 71–80 d after vitamin A supplementation and by 101–110d conversion had taken place in all children. Compared with the placebo group, plasma retinol concentration at 100d post-supplement was found to be significantly higher in the vitamin A-supplemented group P = 0·04. This study demonstrates that CIC responds to a massive oral dose of vitamin A 3–4 months after supplementation. These findings should guide future studies and evaluations in which CIC is used to assess response to vitamin A interventions.

Keywords

Conjunctival impression cytologyVitamin ARetinol
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 863 - 869
Copyright
Copyright © The Nutrition Society 1997

References

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