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Dietary-induced changes in the fatty acid profile of rat pancreatic membranes are associated with modifications in acinar cell function and signalling

Published online by Cambridge University Press:  09 March 2007

Maria D. Yago ,
Ricardo J. Diaz ,
Rolando Ramirez ,
Maria A. Martinez ,
Mariano Mañas  and
Emilio Martinez-Victoria
Maria D. Yago
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
Ricardo J. Diaz
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
Rolando Ramirez
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
Maria A. Martinez
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
Mariano Mañas*
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
Emilio Martinez-Victoria
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/ Ramon y Cajal 4, 18071 Granada, Spain
*
*Corresponding author: Professor Mariano Mañas, fax +34 958 248326, email mariano@ugr.es
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Abstract

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The effects of dietary lipids on the fatty acid composition of rat pancreatic membranes and acinar cell function were investigated. Weaning rats were fed for 8 weeks on one of two diets which contained 100 g virgin olive oil (OO) or sunflowerseed oil (SO)/kg. Pancreatic plasma membranes were isolated and fatty acids determined. Amylase secretion and cytosolic concentrations of Ca2+ and Mg2+ were measured in pancreatic acini. Membrane fatty acids were profoundly affected by the diets; the rats fed OO had higher levels of 18:1n-9 (42·86 (sem 1·99) %) and total MUFA compared with the animals fed SO (25·37 (sem 1·11) %). Reciprocally, the SO diet resulted in greater levels of total and n-6 PUFA than the OO diet. The most striking effect was observed for 18:2n-6 (SO 17·88 (sem 1·32) %; OO 4·45 (sem 0·60) %), although the levels of 20:4n-6 were also different. The proportion of total saturated fatty acids was similar in both groups, and there was only a slight, not significant (P=0·098), effect on the unsaturation index. Compared with the OO group, acinar cells from the rats fed SO secreted more amylase at rest but less in response to cholecystokinin octapeptide, and this was paralleled by reduced Ca2+ responses to the secretagogue. The results confirm that rat pancreatic cell membranes are strongly influenced by the type of dietary fat consumed and this is accompanied by a modulation of the secretory activity of pancreatic acinar cells that involves, at least in part, Ca2+ signalling.

Keywords

Dietary fatPancreatic aciniMembrane fatty acidsCholecystokininCalcium signalling
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 2 , February 2004 , pp. 227 - 234
Copyright
Copyright © The Nutrition Society 2004

References

Akiyama, T, Hirohata, Y, Okabayashi, Y, Imoto, I & Otsuki, M (1998) Supramaximal CCK and CCh concentrations abolish VIP potentiation by inhibiting adenyl cyclase activity. Am J Physiol 275, G1202–G1208.Google Scholar
Alam, SQ, Mannino, SJ & Alam, BS (1993) Reversal of diet-induced changes in adenylate cyclase activity and fatty acid composition of rat submandibular salivary gland lipids. Arch Oral Biol 38, 387–391.CrossRefGoogle ScholarPubMed
Ballesta, MC, Mañas, M, Mataix, FJ, Martinez-Victoria, E & Seiquer, I (1990) Long-term adaptation of pancreatic response by dogs to dietary fats of different degrees of saturation: olive and sunflower oil. Br J Nutr 64, 487–496.Google Scholar
Begin, ME, Ells, G, St-Jean, P, Vachereau, A & Beaudoin, AR (1990) Fatty acid and enzymatic compositional changes in the pancreas of rats fed dietary n-3 and n-6 polyunsaturated fatty acids. Int J Pancreatol 6, 151–160.CrossRefGoogle ScholarPubMed
Berlin, E, Bhathena, SJ, McClure, D & Peters, RC (1998) Dietary menhaden and corn oils and the red blood cell membrane lipid composition and fluidity in hyper- and normocholesterolemic miniature swine. J Nutr 128, 1421–1428.CrossRefGoogle ScholarPubMed
Bourassa, J, Laine, J, Kruse, ML, Gagnon, MC, Calvo, E & Morisset, J (1999) Ontogeny and species differences in the pancreatic expression and localization of the CCKA receptors. Biochem Biophys Res Commun 260, 820–828.CrossRefGoogle Scholar
Bragado, MJ, San Roman, JI, Gonzalez, A, Garcia, LJ, Lopez, MA & Calvo, JJ (1996) Impairment of intracellular calcium homeostasis in the exocrine pancreas after caerulein-induced acute pancreatitis in the rat. Clin Sci 91, 365–369.Google Scholar
Cho, Y & Ziboh, VA (1994) Expression of protein kinase C isozymes in guinea pig epidermis: selective inhibition of PKC-beta activity by 13-hydroxyoctadecadienoic acid-containing diacylglycerol. J Lipid Res 35, 913–921.Google Scholar
Clandinin, MT, Cheema, S, Field, CJ, Garg, ML, Venkatraman, J & Clandinin, TR (1991) Dietary fat: exogenous determination of membrane structure and cell function. FASEB J 5, 2761–2769.CrossRefGoogle ScholarPubMed
Field, CJ, Toyomizu, M & Clandinin, MT (1989) Relationship between dietary fat, adipocyte membrane composition and insulin binding in the rat. J Nutr 119, 1483–1489.CrossRefGoogle ScholarPubMed
Giron, MD, Criado, MD, Lara, A & Suarez, MD (1995) Changes in the fatty acid pattern of plasma fractions of rats fed coconut, olive or sunflower oil. Rev Esp Fisiol 51, 65–69.Google ScholarPubMed
Giron, MD, Lara, A & Suarez, MD (1996) Short-term effects of dietary fats on the lipid composition and desaturase activities of rat liver microsomes. Biochem Mol Biol Int 40, 843–851.Google ScholarPubMed
Gonzalez, A, Schmid, A, Sternfeld, L, Krause, E, Salido, GM & Schulz, I (1999) Cholecystokinin-evoked Ca2+ waves in isolated mouse pancreatic acinar cells are modulated by activation of cytosolic phospholipase A 2, phospholipase D, and protein kinase C. Biochem Biophys Res Commun 261, 726–733.CrossRefGoogle ScholarPubMed
Grynkiewicz, G, Poenie, M & Tsien, RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260, 3440–3450.CrossRefGoogle ScholarPubMed
Jensen, RT, Lemp, GF & Gardner, JD (1982) Interactions of COOH-terminal fragments of cholecystokinin with receptors on dispersed acini from guinea pig pancreas. J Biol Chem 257, 5554–5559.Google Scholar
Lajas, AI, Pozo, MJ, Salido, GM & Pariente, JA (1998) Effect of basic fibroblast growth factor on cholecystokinin-induced amylase release and intracellular calcium increase in male rat pancreatic acinar cells. Biochem Pharmacol 55, 903–908.Google Scholar
Lepage, G & Roy, CC (1986) Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res 27, 114–119.Google Scholar
Meldolesi, J, Jamieson, JD & Palade, GE (1971) Composition of cellular membranes in the pancreas of the guinea pig. J Cell Biol 49, 109–129.CrossRefGoogle ScholarPubMed
Neelands, PJ & Clandinin, MT (1983) Diet fat influences liver plasma-membrane lipid composition and glucagon-stimulated adenylate cyclase activity. Biochem J 212, 573–583.CrossRefGoogle ScholarPubMed
Peikin, SR, Rottman, AJ, Batzri, S & Gardner, JD (1978) Kinetics of amylase release by dispersed acini prepared from guinea pig pancreas. Am J Physiol 235, E743–E749.Google ScholarPubMed
Quiles, JL, Huertas, JR, Mañas, M, Battino, M & Mataix, J (1999) Physical exercise affects the lipid profile of mitochondrial membranes in rats fed virgin olive oil or sunflower oil. Br J Nutr 81, 21–24.CrossRefGoogle ScholarPubMed
Quiles, JL, Huertas, JR, Mañas, M, Ochoa, JJ, Battino, M & Mataix, J (2001) Dietary fat type and regular exercise affect mitochondrial composition and function depending on specific tissue in the rat. J Bioenerg Biomembr 33, 127–134.Google Scholar
Reeves, PG, Nielsen, FH, Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.CrossRefGoogle Scholar
Ricketts, J & Brannon, PM (1994) Amount and type of dietary fat regulate pancreatic lipase gene expression in rats. J Nutr 124, 1166–1171.Google Scholar
Sabb, JE, Godfrey, PM & Brannon, PM (1986) Adaptive response of rat pancreatic lipase to dietary fat: effects of amount and type of fat. J Nutr 116, 892–899.CrossRefGoogle ScholarPubMed
Serrano, P, Yago, MD, Mañas, M, Calpena, R, Mataix, J Martinez-Victoria E (1997) Influence of type of dietary fat (olive and sunflower oil) upon gastric acid secretion and release of gastrin, somatostatin and peptide YY in man. Dig Dis Sci 42, 626–633.CrossRefGoogle ScholarPubMed
Soriguer, FJ, Tinahones, FJ, Monzon, A et al. (2000) Varying incorporation of fatty acids into phospholipids from muscle, adipose and pancreatic exocrine tissues and thymocytes in adult rats fed with diets rich in different fatty acids. Eur J Epidemiol 16, 585–594.Google Scholar
Suarez, A, Faus, MJ & Gil, A (1996 a) Dietary long-chain polyunsaturated fatty acids modify heart, kidney, and lung fatty acid composition in weanling rats. Lipids 31, 345–348.Google Scholar
Suarez, A, Ramirez, MCD, Faus, MJ & Gil, A (1996 b) Dietary long-chain polyunsaturated fatty acids influence tissue fatty acid composition in rats at weaning. J Nutr 126, 887–897.CrossRefGoogle ScholarPubMed
Thomson, AB, Keelan, M, Cheng, T & Clandinin, MT (1993) Delayed effects of early nutrition with cholesterol plus saturated or polyunsaturated fatty acids on intestinal morphology and transport function in the rat. Biochim Biophys Acta 1170, 80–91.CrossRefGoogle ScholarPubMed
Tsunoda, Y, Yoshida, H & Owyang, C (1996) Structural requirements of CCK analogues to differentiate second messengers and pancreatic secretion. Am J Physiol 271, G8–G19.Google Scholar
Vajreswari, A & Narayanareddy, K (1992) Effect of dietary fats on some membrane-bound enzyme activities, membrane lipid composition and fatty acid profiles of rat heart sarcolemma. Lipids 27, 339–343.Google Scholar
Wang, H, Dudley, AW Jr, Dupont, J, Reeds, PJ, Hachey, DL & Dudley, MA (1996) The duration of medium-chain triglyceride feeding determines brush border membrane lipid composition and hydrolase activity in newly weaned rats. J Nutr 126, 1455–1462.Google Scholar
Wisdom, DM, Salido, GM, Baldwin, LM & Singh, J (1996) The role of magnesium in regulating CCK-8-evoked secretory responses in the exocrine rat pancreas. Mol Cell Biochem 154, 123–132.CrossRefGoogle ScholarPubMed
Yago, MD, Gonzalez, MV, Martinez-Victoria, E et al. (1997 a) Pancreatic enzyme secretion in response to test meals differing in the quality of dietary fat (olive oil and sunflowerseed oils) in human subjects. Br J Nutr 78, 27–39.Google Scholar
Yago, MD, Martinez-Victoria, E, Mañas, M, Martinez, MA & Mataix, J (1997 b) Plasma peptide YY and pancreatic polypeptide in dogs after long-term adaptation to dietary fats of different degrees of saturation: olive and sunflower oil. J Nutr Biochem 8, 502–507.CrossRefGoogle Scholar