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Dietary protein:lipid ratio and lipid nature affects fatty acid absorption and metabolism in a teleost larva

Published online by Cambridge University Press:  08 March 2007

Sofia Morais ,
William Koven ,
Ivar Rønnestad ,
Maria Teresa Dinis  and
Luís E. C. Conceição
Sofia Morais*
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
William Koven
Affiliation:
Israel Oceanographic and Limnological Research, The National Centre for Mariculture, PO Box 1212, Eilat 88 112, Israel
Ivar Rønnestad
Affiliation:
Department of Biology, University of Bergen, Allégt 41, N-5007, Bergen, Norway
Maria Teresa Dinis
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Luís E. C. Conceição
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
*
*Corresponding author: Dr Sofia Morais, fax +351 289 818 353, email smorais@ualg.pt
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Abstract

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Studies with teleost larvae have reported poor performance associated with quantitative lipid imbalances in the diet. The present study examined the effect of dietary protein:neutral lipid ratio on fatty acid (FA) absorption efficiency and metabolism in larval Senegalese sole. In addition, the effect of lipid class (triolein (TRI) and l-3-phosphatidylcholine-1,2-di-oleoyl (PC)), carbon number and degree of saturation of the labelled NEFA, stearic acid (SA), oleic acid (OA) and DHA) was tested. FA absorption was determined by tube feeding [1-14C]-labelled lipids and NEFA after a single meal of either non-enriched Artemia (NEA) or Artemia enriched on a soyabean oil emulsion (EA), or after feeding these diets over an extended period of time (18 d). The tested dietary protein:lipid ratios had no short-term influence but long-term feeding of a diet higher in neutral lipid (EA) increased lipid accumulation within the gut epithelium and resulted in lower FA absorption (higher label evacuation and lower retention of dietary FA), which may partially explain the trend for lower growth observed with this diet. The lipids and NEFA, showed different digestive and metabolic properties, independent of feeding regime. FA absorption increased with unsaturation, being lowest for SA, followed by OA, and highest for DHA. In addition, sole larvae had a lower capacity to digest and absorb FA esterified to TRI, compared with PC, with the order of decreasing absorption being NEFA>PC≫TRI. Moreover, larvae appeared to discriminate between the source of OA, as this FA in the free form or esterified to PC was catabolised less than TRI.

Keywords

Solea senegalensisFish larvaeLipid classFatty acid saturation
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 813 - 820
Copyright
Copyright © The Nutrition Society 2005

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