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Determination of the energy expenditure of penned and grazing sheep from estimates of carbon dioxide entry rate

Published online by Cambridge University Press:  19 January 2009

J. L. Corbett ,
D. J. Farrell ,
R. A. Leng ,
G. L. McClymont  and
B. A. Young
J. L. Corbett
Affiliation:
CSIRO, Division of Animal Physiology, Pastoral Research Laboratory, Armidale, NSW, 2350, Australia
D. J. Farrell
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW, 2351, Australia
R. A. Leng
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW, 2351, Australia
G. L. McClymont
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW, 2351, Australia
B. A. Young
Affiliation:
Department of Physiology, University of New England, Armidale, NSW, 2351, Australia
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Abstract

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1. NaH14CO3, was infused at a constant rate either subcutaneously or intraperitoneally into sheep kept in a uniform environment and consuming a standard amount of food each hour. After 3 h of infusion the specific radioactivity (SR) of COe obtained by acidification of samples of blood taken from the right atrium showed no significant change with time until after 22 h when the infusions were stopped.

2. Entry rates of COa (I/h) in sheep were estimated from the ratio of rate of infusion of 14CO2, as NaH14C08 (µCi/h) to the SR of CO2 (µCi/l) in samples of jugular venous blood, urine and exhaled gas taken after infusions had been in progress for at least 3 h. Concurrently, rates of energy expenditure were calculated from the gaseous exchanges over periods of 60–90 min which were determined for grazing sheep by use of re-entrant tracheal cannulation and meter-ing and analysis of exhaled gas, or for housed sheep by measurement in respiration chambers.

3. Significant positive relationships were found between entry rate, the independent variable, and the contemporary energy expenditure. Equations obtained for grazing sheep were not significantly different from those obtained for other, housed, sheep when both groups were infused subcutaneously and COz for assay of radioactivity was obtained in all instances from blood, or in all instances from urine.

4. With subcutaneous infusion the equation relating energy expenditure to entry rate estimated from the SR of urinary COa differed significantly from that where entry rate was derived from radioactivity assays of blood or exhaled CO2. There was also a significant differ-ence between two equations where entry rates were determined from the SR of blood CO2 but route of infusion was in the one instance intravenous and in the other was sub-cutaneous. Reasons for the differences between equations are discussed.

5. Changes in the rates of energy expenditure of sheep effected by intermittent exercise on a treadmill were reflected in changes in CO2 entry rates. Values for the energy cost of hori-zontal locomotion by the sheep were derived from the entry rates and were similar in magni-tude to those reported by other workers.

6. I t is suggested that the method of determining energy expenditure from COz entry rate may be adapted for use on many species of animal in a variety of environments.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 26 , Issue 2 , September 1971 , pp. 277 - 291
Copyright
Copyright © The Nutrition Society 1971

References

REFERENCES

Annison, E. F., Brown, R. E., Leng, R. A., Lindsay, D. B. & West, C. E. (1967). Biochem. J. 104, 135.CrossRefGoogle Scholar
Bergman, E. N. & Hogue, D. E. (1967). Am. J. Physiol. 213, 1378.CrossRefGoogle Scholar
Brockway, J. M. & McEwan, E. H. (1969). J. Physiol., Lond. 202, 661.CrossRefGoogle Scholar
Brouwer, E. (1958). Publs Eur. Ass. Anim. Prod. no. 8, p. 182.Google Scholar
Brouwer, E. (1965). In Energy Metabolism p. 441 [Blaxter, K. L., editor]. London: Academic Press.Google Scholar
Buchanan, D. L. (1950–1951). J. gen. Physiol. 34, 737.CrossRefGoogle Scholar
Buchanan, D. L. & Nakao, A. (1952). J. biol. Chem. 198, 245.CrossRefGoogle Scholar
Clapperton, J. L. (1964). Br. J. Nutr. 18, 47.CrossRefGoogle Scholar
Corbett, J. L., Leng, R. A. & Young, B. A. (1969). In Energy Metabolism of Farm Animals p. 177 [Blaxter, K. L., Kielanowski, J. & Thorbek, G., editors]. Newcastle upon Tyne: Oriel Press.Google Scholar
Farrell, D. J., Corbett, J. L. & Leng, R. A. (1970). Res. vet. Sci. 11, 217.CrossRefGoogle Scholar
Flatt, W. P., Waldo, D. R., Sykes, J. E. & Moore, L. A. (1958). Publs Eur. Ass. Anim. Prod. no. 8, p. 101.Google Scholar
Graham, N. McC. (1962). Proc. Aust. Soc. Anim. Prod. 4, 138.Google Scholar
Graham, N. McC. (1967). Aust. J. agric. Res. 18, 467.Google Scholar
Heinemann, H. O. & Fishman, A. P. (1969). Physiol. Rev. 49, 1.CrossRefGoogle Scholar
Hoernicke, H., Williams, W. F., Waldo, D. R. & Flatt, W. P. (1965). In Energy Metabolism p. 165 [Blaxter, K. L., editor]. London: Academic Press.Google Scholar
Huber, T. L., Mayfield, E. D., Huston, R. L. & Johnson, B. C. (1965). Proc. Soc. exp. Biol. Med. 120, 214.CrossRefGoogle Scholar
Leng, R. A., Corbett, J. L. & Brett, D. J. (1968). Br. J. Nutr. 22, 57.CrossRefGoogle Scholar
Leng, R. A. & Leonard, G. J. (1965). Br. J. Nutr. 19, 469.CrossRefGoogle Scholar
Lifson, N., Gordon, G. B. & McClintock, R. (1955). J. appl. Physiol. 7, 704.CrossRefGoogle Scholar
Lifson, N. & McClintock, R. (1966). J. theoret. Biol. 12, 46.CrossRefGoogle Scholar
Prosser, C. L. & Brown, F. A. (1962). Comparative Animal Physiology. Philadelphia: W. B. Saunders.CrossRefGoogle Scholar
Webster, A. J. F. (1967). Br. J. Nutr. 21, 769.CrossRefGoogle Scholar
White, R. G. & Leng, R. A. (1968). Proc. Aust. Soc. Anim. Prod. 7, 335.Google Scholar
Young, B. A. (1968). Maintenance energy requirement of grazing sheep. PhD Thesis, University of New England.Google Scholar
Young, B. A. (1970). Publs Eur. Ass. Anim. Prod. no. 13, p. 237.Google Scholar
Young, B. A. & Corbett, J. L. (1968). Proc. Aust. Soc. Anim. Prod. 7, 327.Google Scholar
Young, B. A., Leng, R. A., White, R. G., McClymont, G. L. & Corbett, J. L. (1969). In Energy Metabolism of Farm Animals p. 435 [Blaxter, K. L.Kielanowski, J. and Thorbek, G., editors]. Newcastle upon Tyne: Oriel Press.Google Scholar
Young, B. A. & Webster, M. E. D. (1963). Aust. J. agric. Res. 14, 867.CrossRefGoogle Scholar