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Degradation of the nucleic acids in mixed rumen bacteria on incubation with different media

Published online by Cambridge University Press:  13 December 2007

R. C. Smith  and
R. H. Smith
R. C. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
R. H. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
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Abstract

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1. Mixed rumen bacteria were grown in vitro in a medium containing [U-14C]adenine. Radioactivity was incorporated into the purine bases of the nucleic acids.

2. Labelled bacteria were incubated with a nitrogen-deficient cell-free medium (medium A), a protozoa-free supernatant suspension from a slow-speed centrifuging of a steer's rumen contents (medium B) and whole rumen contents of a steer (medium C). Mean (six experiments) proportional rates of release of radioactivity(/h) were significantly greater for medium A (0.140) than for media B or C (0.055 and 0.043 respectively). Some of the radioactivity accumulated in the cell-free media; some was lost, partially as carbon dioxide.

3. Calculation from this and other information indicated that about 30% of bacterial nucleic acids may turn over in the rumen even in the absence of protozoa.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 37 , Issue 3 , May 1977 , pp. 389 - 394
Copyright
Copyright © The Nutrition Society 1977

References

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