Hostname: page-component-77c89778f8-vpsfw Total loading time: 0 Render date: 2024-07-22T20:10:49.607Z Has data issue: false hasContentIssue false
  • English
  • Français

Degradation of hemicellulose and pectin by horse caecum contents

Published online by Cambridge University Press:  09 March 2007

A. Bonhomme-Florentin
A. Bonhomme-Florentin
Affiliation:
L.A. CNRS n° 138, Groupe de Zoologie et Protistologie, les Cézeaux, BP 45, 63170 Aubiere, and Laboratoire de Zoologie, U.F.R. Sciences, BP 347, 51062 Reims-Cédex, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Polysaccharide depolymerases and glycoside hydrolases involved in the breakdown of plant structural polysaccharides (hemicellulose and pectins) were monitored in three fractions of the liquid phase of horse caecum digesta: acellular fluid (AF), bacteria (B) and protozoa plus bacteria (PB).

2. Both bacteria and protozoa were found to be involved in the decomposition of pectic substances, with two enzymic activities: depolymerase (polygalacturonase, EC 3.2.1.15; and pectin lyase, EC 4.2.2.10) and esterase (pectinesterase, EC 3.1.1.11). The activity of the PB fraction was higher than that of B.

3. With hemicellulosic substrates, all three fractions showed a significant xylan endo-1,3-β-xylosidase (EC 3.2.1.32) activity. Mannan was hardly broken down.

4. Galactomannan and arabinogalactan were broken down more extensively by the PB fraction than by the B fraction. Glycosidase activities (xylan 1,4-β-xylosidase, EC 3.2.1.37 and α-L-arabinofuranosidase, EC 3.2.1.55) were also observed.

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 60 , Issue 1 , July 1988 , pp. 185 - 192
Copyright
Copyright © The Nutrition Society 1988

References

Abou Akkada, A. R., Eadie, J. M. &, Howard, B. H. (1963). Biochemical Journal 89, 268–272.CrossRefGoogle Scholar
Abou Akkada, A. R. &, El Shazly, K. (1965). Journal of Agricultural Science, Cambridge 64, 251–255.CrossRefGoogle Scholar
Abou Akkada, A. R. &, Howard, B. H. (1961). Biochemical Journal 78, 512–517.CrossRefGoogle Scholar
Bailey, R. W., Clarke, R. T. &, Wright, D. E. (1962). Biochemical Journal 83, 517–523.CrossRefGoogle Scholar
Bonhomme-Florentin, A. (1974). Annales des Sciences Naturelles 12, 155–283.Google Scholar
Bonhomme-Florentin, A. (1985). Reproduction, Nutrition Développement 25, 127–139.CrossRefGoogle Scholar
Bonhomme-Florentin, A. (1986 a). Reproduction, Nutrition Développement 26, 291–292.CrossRefGoogle Scholar
Bonhomme-Florentin, A. (1986 b). Japan Journal of Veterinary Science 48 (2), 313–322.Google Scholar
Clarke, R. T. (1977). In Microbiology of the Gut, pp. 251–275. [Clarke, R.T. and Bauchop, T., editors]. London: Academic Press.Google Scholar
Dehority, B. A. (1965). Journal of Bacteriology 89, 1515–1520.CrossRefGoogle Scholar
Dehority, B. A. (1973). Federation Proceedings 32, 1819–1825.Google Scholar
Grenet, E., Martin-Rosset, W. &, Chenost, M. (1984). Canadian Journal of Animal Science 64, 345–346.CrossRefGoogle Scholar
Harbers, L. H., McNally, L. K. &, Smith, W. H. (1981). Journal of Animal Science 53, 1671–1676.CrossRefGoogle Scholar
Hess, K. &, Philipoff, W. (1937). Berichte der Deutschen chemischen Gessellschaft 70, 639–659.CrossRefGoogle Scholar
Hintz, H. F. (1969). Veterinarian 6, 45–50.Google Scholar
Hungate, R. E. (1942). Biological Bulletin 83, 303–319.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. &, Randall, R. J. (1951). Journal of Biological Chemistry 193, 265–275.CrossRefGoogle Scholar
MacIlvaine, T. C. (1921). Journal of Biological Chemistry 49, 183–188.CrossRefGoogle Scholar
Mah, R. A. &, Hungate, R. E. (1965). Journal of Protozoology 12, 131–136.CrossRefGoogle Scholar
Nelson, N. (1944). Journal of Biological Chemistry 153, 375–380.CrossRefGoogle Scholar
Peltonen, T., Kossila, V., Syrjala, L. &, Immonen, I. (1978). Annales Agriculturae Fenniae 17, 109–114.Google Scholar
Prins, R. A. (1977). In Microbial Ecology of the Gut, pp. 73–183 [Clarke, R.T., Bauchop, T., editors]. London: Academic Press.Google Scholar
Trevelyan, W. E., Procter, D. P. &, Harrison, J. S. (1950). Nature 166, 444–445.CrossRefGoogle Scholar
Williams, A. G. &, Strachan, N. H. (1984). Current Microbiology 10, 215–220.CrossRefGoogle Scholar
Wright, D. E. (1960). Archives of Biochemistry and Biophysics 86, 251–254.CrossRefGoogle Scholar
Wright, D. E. (1961). New Zealand Journal of Agricultural Research 4, 203–215.CrossRefGoogle Scholar