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The amino acid composition of human milk corrected for amino acid digestibility

Published online by Cambridge University Press:  22 August 2007

Alison J. Darragh  and
Paul J. Moughan
Alison J. Darragh
Affiliation:
Milk and Health Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand
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Abstract

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Human milk was collected from women in their 10th–14th weeks of lactation, and was analysed for amino acids. Corrections were made for losses of amino acids which were presumed to occur during acid hydrolysis, using a non-linear mathematical model that describes the simultaneous processes of amino acid yield and decay. The mean amino acid composition of the human milk was found to be similar to previously reported estimates, although the cysteine content of the human milk in the present study was 20% higher than the average literature estimate. True (corrected for endogenous amino acid excretions) ileal amino acid digestibility of human milk was determined using the 3-week-old piglet as a model animal for the human infant. The piglets were given either human milk (n 6) or a protein-free diet (n 6) for a 6 d experimental period. Cr2O3 was added as an indigestible marker, to both the human milk and protein-free diet. At the end of the experimental period the piglets were anaesthetized and samples of digesta removed from the terminal ileum of each piglet. After sampling the piglets were killed. Endogenous ileal excretions of amino acids were determined in piglets fed on the protein-free diet. The true digestibilities of total N and amino acid N were 88% and 95% respectively. The true ileal digestibility of the non-amino acid N fraction in human milk, when calculated by difference was only 50%. The true digestibility of the amino acids in human milk ranged from 81–101% with threonine (86%) being the least digestible essential amino acid. When the true ileal digestibility values were used to correct the amino acid composition of human milk, the pattern of digestible amino acids in human milk was different compared with the currently recommended pattern of amino acid requirements for the infant.

Keywords

Human milkAmino acidsDigestionAnimal modelPiglet
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 1 , July 1998 , pp. 25 - 34
Copyright
Copyright © The Nutrition Society 1998

References

Association of Official Analytical Chemists (1980) Official Methods of Analysis, vol. 14. Washington, DC: Association of Official Analytical Chemists.Google Scholar
Brock, JH, Arzabe, F, Lampreave, F & Pineiro, A (1976) The effect of trypsin on bovine transferrin and lactoferrin. Biochimica et Biophysica Acta 446, 214–225.CrossRefGoogle ScholarPubMed
Butte, NF, Garza, C, Burr, R, Goldman, AS, Kennedy, K & Kitzmiller, JL (1987) Milk composition of insulin-dependent diabetic women. Journal of Pediatric Gastroenterology and Nutrition 6, 936–941.Google ScholarPubMed
Butts, CA, Moughan, PJ, Smith, WC & Carr, DH (1993) Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): the effect of protein-free, synthetic amino acid, peptide and protein alimentation. Journal of the Science of Food and Agriculture 61, 31–40.CrossRefGoogle Scholar
Casey, CE & Hambidge, KM (1983) Nutritional aspects of human lactation. In Lactation Physiology, Nutrition and Breast Feeding, pp. 85–87 [Neville, MC and Neifert, MR, editors]. New York, NY: Plenum Press.Google Scholar
Committee on Nutrition (1985) Infant nutrition and the development of gastrointestinal function. In Pediatric Nutrition Handbook, pp. 144–153 [Forbes, GB and Woodruff, CW, editors]. Illinois: American Academy of Pediatrics.Google Scholar
Costigan, P & Ellis, KJ (1987) Analysis of faecal chromium derived from controlled-release marker devices. New Zealand Journal of Technology 3, 89–92.Google Scholar
Darragh, AJ (1995) The amino acid composition of human milk – towards determining the amino acid requirements of the human infant.Google Scholar
Darragh, AJ, Cranwell, PD & Moughan, PJ (1994) Absorption of lysine and methionine from the proximal colon of the piglet. British Journal of Nutrition 71, 739–752.CrossRefGoogle ScholarPubMed
Darragh, AJ, Garrick, DG, Moughan, PJ & Hendriks, WH (1996) Correction for amino acid loss during acid hydrolysis of a purified protein. Analytical Biochemistry 236, 199–207.CrossRefGoogle ScholarPubMed
Darragh, AJ & Moughan, PJ (1995) The three-week-old piglet as a model animal for studying protein digestion in human infants. Journal of Pediatric Gastroenterology and Nutrition 21, 387–393.Google Scholar
Davidson, LA & Lönnerdal, B (1987) Persistence of human milk proteins in the breast-fed infant. Acta Paediatrica Scandinavica 76, 733–740.CrossRefGoogle ScholarPubMed
Department of Health and Social Security (1977) The Composition of Mature Human Milk. Report on Health and Social Subjects, no. 12. London: HM Stationery Office.Google Scholar
Department of Health and Social Security (1981) The Collection and Storage of Human Milk. Report on Health and Social Subjects, no. 22. London: HM Stationery Office.Google Scholar
Dewey, KG, Beaton, G, Fjeld, C, Lönnerdal, B & Reeds, P (1996) Protein requirements of infants and children. European Journal of Clinical Nutrition 50, Suppl. 1, S119–S150.Google ScholarPubMed
Food and Agriculture Organization/World Health Organization/United Nations University (1985) Energy and Protein Requirements. WHO Technical Report Series, no. 724. Geneva: World Health Organization.Google Scholar
Food and Agriculture Organization/World Health Organization/United Nations University (1990) Joint FAO/WHO Expert Consultation of Protein Quality Evaluation. Rome: FAO.Google Scholar
Fransson, G-B & Lönnerdal, B (1980) Iron in human milk. Journal of Pediatrics 96, 380–384.CrossRefGoogle ScholarPubMed
Garza, C, Johnson, CA, Harrist, R & Nichols, B (1982) Effects of methods of collection and storage on nutrients in human milk. Early Human Development 6, 295–303.CrossRefGoogle ScholarPubMed
Hambraeus, L, Fransson, G-B & Lönnerdal, B (1984) Nutritional availability of breast milk protein. Lancet 2, 167–168.CrossRefGoogle ScholarPubMed
Hambraeus, L, Lönnerdal, B, Forsum, E & Gebre-Medhin, M (1978) Nitrogen and protein components of human milk. Acta Paediatrica Scandinavica 67, 561–565.CrossRefGoogle ScholarPubMed
Hamosh, M, Berkow, S, Bitman, J, Freed, L, Happ, B, Jones, JB, Mehta, NR, Wood, DL & Hamosh, P (1984) Handling and storage of human milk specimens for research. Journal of Pediatric Gastroenterology and Nutrition 3, 284–289.Google ScholarPubMed
Haneberg, B & Tonder, O (1973) Immunoglobulins and other serum proteins in feces from infants and children. Scandinavian Journal of Immunology 2, 375–383.CrossRefGoogle ScholarPubMed
Harzer, G & Bindels, JG (1987) Main compositional criteria of human milk and their implications on nutrition in early infancy. In New Aspects of Nutrition in Pregnancy, Infancy and Prematurity, pp. 83–94 [Xanthou, M, editor]. Amsterdam: Elsevier Science Publishers.Google Scholar
Hytten, FE (1954) Clinical and chemical studies in human lactation: III. Diurnal variation in major constituents of milk. British Medical Journal i, 179–182.Google Scholar
Jackson, AA (1989) Optimizing amino acid and protein supply and utilization in the newborn. Proceedings of the Nutrition Society 48, 293–301.CrossRefGoogle ScholarPubMed
Lönnerdal, B (1985) Biochemistry and physiological function of human milk proteins. American Journal of Clinical Nutrition 42, 1299–1317.CrossRefGoogle ScholarPubMed
Lönnerdal, B (1986) Effects of maternal dietary intake on human milk composition. Journal of Nutrition 116, 499–513.CrossRefGoogle ScholarPubMed
Lönnerdal, B & Chen, C-L (1990) Effects of formula protein level and ratio on infant growth, plasma amino acids and serum trace elements. I. Cow's milk formula. Acta Paediatrica Scandinavica 79, 257–265.CrossRefGoogle ScholarPubMed
Lönnerdal, B, Forsum, E, Gebre-Medhin, M & Hambraeus, L (1976) Breast milk composition in Ethiopian and Swedish mothers. II. Lactose, nitrogen and protein content. American Journal of Clinical Nutrition 29, 1134–1141.CrossRefGoogle Scholar
Low, AG (1980) Nutrient absorption in pigs. Journal of the Science of Food and Agriculture 31, 1087–1130.CrossRefGoogle ScholarPubMed
McNeil, NI (1988) Nutritional implication of human and mammalian large intestinal function. World Review of Nutrition and Dietetics 56, 1–42.CrossRefGoogle ScholarPubMed
Moore, S (1963) On the determination of cystine as cysteic acid. Journal of Biological Chemistry 238, 235–237.CrossRefGoogle Scholar
Moughan, PJ, Birtles, MJ, Cranwell, PD, Smith, WC & Pedraza, M (1992 a) The piglet as a model animal for studying aspects of digestion and absorption in milk-fed human infants. World Review of Nutrition and Dietetics 67, 40–113.CrossRefGoogle Scholar
Moughan, PJ & Schuttert, G (1991) Composition of nitrogen-containing fractions in digesta from the distal ileum of pigs fed a protein-free diet. Journal of Nutrition 121, 1570–1574.CrossRefGoogle ScholarPubMed
Moughan, PJ, Schuttert, G & Leenaars, M (1992 b) Endogenous amino acid flow in the stomach and small intestine of the young growing pig. Journal of the Science of Food and Agriculture 60, 437–442.CrossRefGoogle Scholar
Ogra, SS, Weintraub, D & Ogra, PL (1977) Immunologic aspects of human colostrum and milk. III. Fate and absorption of cellular and soluble components in the gastrointestinal tract of the newborn. Journal of Immunology 119, 245–248.CrossRefGoogle ScholarPubMed
Prentice, A, MacCarthy, A, Stirling, DM, Vasquez-Valesquez, L & Ceesay, SM (1989) Breast-milk IgA and lactoferrin survival in the gastrointestinal tract – a study in rural Gambian children. Acta Paediatrica Scandinavica 78, 505–512.CrossRefGoogle ScholarPubMed
Räihä, N, Minoli, I, Moro, G & Bremer, HJ (1986) Milk protein intake in the term infant. II. Effect on plasma amino acid concentrations. Acta Paediatrica Scandinavica 75, 887–892.CrossRefGoogle ScholarPubMed
Robel, EJ & Crane, AB (1972) An accurate method for correcting unknown amino acid losses from protein hydrolysates. Analytical Biochemistry 48, 233–246.CrossRefGoogle Scholar
Rodwell, VW (1985) Catabolism of the carbon skeletons of amino acids. In Harper's Review of Biochemistry, 20th ed. California: Lange Medical Publications.Google Scholar
Rowan, AM, Moughan, PJ, Wilson, MN, Maher, K & Tasman-Jones, C (1994) Comparison of the ileal and faecal digestibility of dietary amino acids in adult humans and evaluation of the pig as a model animal for digestion studies in man. British Journal of Nutrition 71, 29–42.CrossRefGoogle Scholar
Sauer, WC & de Lange, K (1992) Novel methods for determining protein and amino acid digestibilities in feedstuffs. In Modern Methods in Protein Nutrition and Metabolism, pp. 87–119 [Nissen, S, editor]. New York, NY: Academic Press.CrossRefGoogle Scholar
Sauer, WC & Ozimek, L (1986) Digestibility of amino acids in swine: results and their practical application. A review. Livestock Production Science 15, 367–388.CrossRefGoogle Scholar
Schmitz, M, Ahrens, F, Schön, J & Hagemeister, H (1991) Amino acid absorption and its significance for protein supply. In Digestive Physiology in Pigs, pp. 85–87 [Verstegen, MWA, Huisman, J and Den Hartog, LA, editors]. Wageningen: Pudoc Publishing.Google Scholar
Spik, G, Brunet, B, Mazurier-Dehaine, C, Fontaine, G & Montreuil, J (1982) Characterization and properties of the human and bovine lactotransferrins extracted from the faeces of newborn infants. Acta Paediatrica Scandinavica 71, 979–985.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems (1985) SAS User's Guide: Statistics. Cary, NC: SAS Institute Inc.Google Scholar
Sturman, JA, Gaull, G & Räihä, NCR (1970) Absence of cystathionase in human fetal liver: is cystine essential? Science 169, 74–76.CrossRefGoogle ScholarPubMed
Svanberg, U, Gebre-Medhin, M, Ljungqvist, B & Olsson, M (1977) Breast milk composition in Ethiopian and Swedish mothers. III. Amino acids and other nitrogenous substances. American Journal of Clinical Nutrition 30, 499–507.CrossRefGoogle ScholarPubMed
van Woelderen, BF (1987) Changing insights into human milk proteins: some implications. Nutrition Abstracts and Reviews (Series A) 57, 129–134.Google Scholar