Some routine handling procedures can produce stress in farm animals, and an adequate control of these stressors is important to avoid the negative effects on animal health and production. The measurement of biomarkers in saliva can be a suitable tool for the evaluation and control of stress. In this report, lipase, butyrylcholinesterase (BChE), total esterase (TEA) and adenosine deaminase (ADA) activities in the saliva of sheep were evaluated as biomarkers of stress. For this purpose, they were measured after inducing stress by facing a dog (experiment 1) and shearing (experiment 2), and comparing them to other stress salivary biomarkers such as α-amylase (sAA) and cortisol, as well as heart rate (HR). Each analyte was measured at the basal time, and during and just after the end of the stressful stimulus, and at various times for the first hour after the period of stress induction. Values were compared with those obtained from a control group. Lipase was the only analyte that showed significant changes between the stress and the control group in both experiments. Although TEA and ADA increased after stress, no significant differences were seen compared with the control group. Lipase was correlated highly with sAA and HR, in experiment 1; and correlated moderately with cortisol and HR in experiment 2. Lipase showed the greatest percentage increase after the stressful stimuli and less overlap with the control group in the two experiments. From the results of this study it can be concluded that lipase, TEA, BChE and ADA are enzymes present in the saliva of sheep and that they can be measured by using simple and fast colorimetric methods. Further studies should be undertaken with regard to the possible application of lipase as a biomarker of stress in sheep.

Commercial broiler production systems based on market initiatives to improve animal welfare beyond minimum legal requirements have emerged in several European countries. A common factor in the ‘higher welfare’ indoor systems is the application of environmental enrichment, with or without natural light, to promote locomotor activity and natural behaviours of the broiler chickens. In the current study, we evaluated the effects of a commercial enrichment programme for fast-growing indoor-housed broiler chickens, with or without natural light entering the broiler house. Enrichment materials were selected in relation to perceived minimal hygiene risk and ease of cleaning in between production cycles. Selected enrichments were a combination of wood shavings bales (1.5 bale/1000 chickens), round metal perches (2.7 m/1000 chickens) and metal chains as pecking objects (1/1000 chickens). Three treatments were studied: control (C) without enrichment and natural light, enriched (E) with enrichments as previously defined but without natural light and enriched plus natural light (EL) with enrichments as previously defined and natural light entrance. The experiment was carried out during five subsequent production cycles on one commercial broiler farm with three identical houses. EL could only be assigned to the middle house that was equipped with roof windows (light entrance area: 3% of floor space). C and E were in the two outer houses (alternated in between production cycles). Behaviour was observed during daytime at days 25 and 39 of age by scan sampling. Lameness, footpad dermatitis, hock burn, cleanliness and injuries were scored at the same ages, in addition to the response of the chickens to a novel object. Results showed that the treatments only affected broiler behaviour. E flocks showed significantly more resting as compared with EL and C. EL flocks showed significantly more walking, exploration and foraging behaviour as compared with E and C. Thus, broiler activity was highest in the EL treatment and lowest in the E treatment, with the C treatment in between. No treatment effects were found on the other welfare indicators and only a few tendencies for treatment effects were found for the novel object test, with E birds tending to be more reluctant to approach the object as compared with EL and C birds. We concluded that providing environmental enrichment and natural light-stimulated activity and natural behaviours in broiler chickens, whereas providing enrichment only seemed to have the opposite effect as compared with control flocks without enrichment.

In Germany all keepers of livestock are legally required to record animal welfare indicators as part of their on-farm self-assessment. The Association for Technology and Structures in Agriculture (Kuratorium für Technik und Bauwesen in der Landwirtschaft e.V. (KTBL)) has suggested the use of a particular set of animal welfare indicators in their publication Animal welfare indicators: Practical guide – Pigs. The aim of the present study was to evaluate the inter-observer reliability (Inter-OR) and intra-observer reliability (Intra-OR) of these indicators with respect to the welfare of fattening pigs. For the assessment of Inter-OR, three observers evaluated six KTBL animal welfare indicators. The Inter-OR of the indicators was calculated from the results using intra-class correlation coefficients (ICC). ‘Excellent’ Inter-OR results were found for the indicators tail length (ICC 0.89), skin lesions (ICC 0.77) and ear lesions (ICC 0.80). In contrast, the Inter-OR of the indicators tail lesions (ICC 0.46) and faecal soiling (ICC 0.47) were considered to be only ‘fair’ and that of the indicator lameness (ICC 0.36) as ‘poor’. For the evaluation of the Intra-OR, the same three observers assessed the welfare of 162 to 200 fattening pigs using the same welfare indicators in total eight times. Again ICCs, here per indicator and observer, were used to calculate the Intra-OR. The Intra-OR of the indicators faecal soiling (ICC 0.81) and ear lesions (ICC 0.97) lay in the ‘excellent’ range on average. While the Intra-OR of the indicators skin lesions (ICC 0.67), tail length (ICC 0.74) and lameness (ICC 0.60) could still be considered as being ‘good’, the Intra-OR of the indicator tail lesions (ICC 0.52) could only be assessed as being ‘fair’. From these results the significance of the KTBL indicators could be judged as follows: it is possible to use all the chosen indicators apart from the indicator tail lesions as an internal controlling instrument or as part of an internal weak-point analysis as long as the indicators are evaluated by the same person. A comparison of the indicators tail lesions, lameness and faecal soiling when assessed by different observers should be considered critically because the Inter-OR of these three indicators could only be considered as being ‘poor’ to ‘fair’.

A two-year (2015 and 2016) grazing study was established to compare ewe and lamb performance when grazed on a perennial ryegrass only sward compared to more diverse sward types. In that study four sward types were investigated: a perennial ryegrass (Lolium perenne) only sward receiving 163 kg nitrogen per hectare per year (N/ha/yr) (PRG); a perennial ryegrass and white clover (Trifolium repens) sward receiving 90 kg N/ha/yr (PRGWC); a six species sward (two grasses (perennial ryegrass and timothy (Phleum pratense)), two legumes (white and red clover (Trifolium pratense)) and two herbs (ribwort plantain (Plantago lanceolata) and chicory (Cichorium intybus)) receiving 90 kg N/ha/yr (6S); and a nine species sward containing cocksfoot (Dactylis glomerata), greater birdsfoot trefoil (Lotus pedunculatus) and yarrow (Achillea millefolium) in addition to the six species listed above, receiving 90 kg N/ha/yr (9S). Each sward type was managed as a separate farmlet and stocked with 30 twin-rearing ewes at a stocking rate of 12.5 ewes/ha under rotational grazing management from turnout post-lambing until housing. Lamb live weight was recorded fortnightly and lambs were drafted for slaughter at 45 kg. Ewe live weight and body condition score (BCS) were recorded on five occasions annually. Lamb faecal egg count (FEC) was recorded fortnightly and lambs were treated with anthelmintics when mean lamb FEC per sward type was above 400 eggs per gram. Ewes grazing the 6S and 9S swards had heavier (P < 0.01) live weights and BCS throughout the study than the ewes grazing the PRG sward. Lambs grazing the 6S sward were heavier than lambs grazing all other sward types of 14 weeks old (P < 0.05). Lambs grazing the PRG sward required more days to reach slaughter weight than lambs grazing all other sward types (P < 0.001). Lambs grazing the 6S and 9S swards required fewer anthelmintic treatments than lambs grazing the PRG or PRGWC swards. In conclusion, grazing multispecies swards improved ewe and lamb performance and reduced the requirement for chemical anthelmintics.

The model LiGAPS-Beef (Livestock simulator for Generic analysis of Animal Production Systems – Beef cattle) has been developed to assess potential and feed-limited growth and production of beef cattle in different areas of the world and to identify the processes responsible for the yield gap. Sensitivity analysis and evaluation of model results with experimental data are important steps after model development. The first aim of this paper, therefore, is to identify which parameters affect the output of LiGAPS-Beef most by conducting sensitivity analyses. The second aim is to evaluate the accuracy of the thermoregulation sub-model and the feed intake and digestion sub-model with experimental data. Sensitivity analysis was conducted using a one-at-a-time approach. The upper critical temperature (UCT) simulated with the thermoregulation sub-model was most affected by the body core temperature and parameters affecting latent heat release from the skin. The lower critical temperature (LCT) and UCT were considerably affected by weather variables, especially ambient temperature and wind speed. Sensitivity analysis for the feed intake and digestion sub-model showed that the digested protein per kg feed intake was affected to a larger extent than the metabolisable energy (ME) content. Sensitivity analysis for LiGAPS-Beef was conducted for ¾ Brahman×¼ Shorthorn cattle in Australia and Hereford cattle in Uruguay. Body core temperature, conversion of digestible energy to ME, net energy requirements for maintenance, and several parameters associated with heat release affected feed efficiency at the herd level most. Sensitivity analyses have contributed, therefore, to insight which parameters are to be investigated in more detail when applying LiGAPS-Beef. Model evaluation was conducted by comparing model simulations with independent data from experiments. Measured heat production in experiments corresponded fairly well to the heat production simulated with the thermoregulation sub-model. Measured ME contents from two data sets corresponded well to the ME contents simulated with the feed intake and digestion sub-model. The relative mean absolute errors were 9.3% and 6.4% of the measured ME contents for the two data sets. In conclusion, model evaluation indicates the thermoregulation sub-model can deal with a wide range of weather conditions, and the feed intake and digestion sub-model with a variety of feeds, which corresponds to the aim of LiGAPS-Beef to simulate cattle in different beef production systems across the world.

Intensive fattening of late-maturing breeds on concrete or rubberized slatted floors is the prevalent beef production system in mainland Europe. The rationale behind this study is that specific beef breeds with different slaughter weights might have a diverse response to different flooring systems. The study aimed at assessing whether growth performance, health, behaviour and claw condition of two beef breeds, Charolais (CH) and Limousine (LIM), would be affected by their housing on concrete (CS) or rubber-covered (RCS) fully slatted floor. A total of 228 CH (116 on CS; 112 on RCS) and 115 LIM (57 on CS; 58 on RCS) were housed in four and two commercial farms, respectively, in groups of 9.0 ± 2.1 animals/pen with an average space allowance of 3.1 ± 0.2 m2. Draining gaps of CS and RCS pens were 16.9 ± 1.7% and 11.6 ± 1.2% of the total surface, respectively. Bulls of both breeds had similar initial body weight (429.4 ± 31.5 kg for CH; 369.6 ± 31.7 kg for LIM), and they were slaughtered when they reached suitable finishing. Charolais had a higher final body weight (BW) than LIM (750.8 ± 8.6 v. 613.7 ± 10.9 kg; P < 0.01), and bulls of both breeds on RCS had higher average daily gain than on CS (1.47 ± 0.02 v. 1.39 ± 0.02 kg/day; P < 0.05). The percentage of bulls early culled or treated for locomotor disorders were reduced by RCS only for LIM, while RCS tended to prevent the occurrence of bursitis for both breeds. During two 8-h behavioural observations, bulls on RCS performed more head butt/displacements and chases than on CS, and they reduced the frequency of abnormal lying down events. The use of RCS increased mounts’ frequency only in LIM, while its reduced drainage capacity impaired only the cleanliness of CH. Postmortem hoof inspection showed longer claw dorsal wall and diagonal lengths, and sharper toe angles for CH on RCS than LIM on both floors. Results of this study point out that fully slatted floors, regardless of being rubberized or not, are not suitable for bulls finished at a final BW above 700 kg due to their detrimental effects on health and welfare. The use of RCS could be recommended as an alternative to CS only if bulls are slaughtered at a lower final BW (around 600 kg), like in the case of LIM breed.

Intensive silvopastoral systems (ISS) are a sustainable alternative to monoculture systems (MS). The presence of trees and legumes improves animal welfare due to the increased food quality and quantity and the presence of shade while providing a variety of environmental services. As cattle behaviour is greatly affected by environmental conditions, knowledge on the behavioural trade-offs that cattle make to meet their demands while foraging in different grazing systems is important, as this will help us understand the perceived advantages of ISS. This pilot study assessed the behaviour of heifers in an ISS (n=8 heifers) and MS (n=8 heifers) in the Mexican tropics during the dry and rainy seasons, and its relationship with forage availability, mean travelled distance and the temperature humidity index (THI). In both seasons, daily foraging times were longer in the MS than the ISS (P<0.01). The duration of rumination was higher for ISS (P<0.01) and the duration of lying was higher for the dry season (P<0.05). The decrease in foraging times in relation to THI was significantly higher in the ISS than in the MS (mean slope±SE: ISS=−4.64±0.34; MS=−2.34±0.22; t=−14.20, P<0.001). The results suggest that the forage availability and access to shade in the ISS allow cattle to rest longer and increase rumination, whereas cattle in MS spend more time searching for food and foraging at times of the day were the temperatures were higher as a compensatory strategy, which potentially decreases cattle’s welfare and production qualities when compared with the ISS. In conclusion, ISS are likely to generate positive behavioural trade-offs that result in better welfare conditions and higher productive potential.

The number of farmed fish in the world has increased considerably. Aquaculture is a growing industry that will in the future provide a large portion of fishery products. Moreover, in recent years, the number of teleost fish used as animal models for scientific research in both biomedical and ecological fields has increased. Therefore, it is increasingly important to implement measures designed to enhance the welfare of these animals. Currently, a number of European rules exist as requirements for the establishment, care and accommodation of fish maintained for human purposes. As far as (teleost) fish are concerned, the fact that the number of extant species is much greater than that of all other vertebrates must be considered. Of further importance is that each species has its own specific physical and chemical requirements. These factors make it difficult to provide generalized recommendations or requirements for all fish species. An adequate knowledge is required of the physiology and ecology of each species bred. This paper integrates and discusses, in a single synthesis, the current issues related to fish welfare, considering that teleosts are target species for both aquaculture and experimental models in biological and biomedical research. We first focus on the practical aspects, which must be considered when assessing fish welfare in both research and aquaculture contexts. Next, we address husbandry and the care of fish housed in research laboratories and aquaculture facilities in relation to their physiological and behavioural requirements, as well as in reference to the suggestions provided by European regulations. Finally, to evaluate precisely which parameters described by Directive 2010/63/EU are reported in scientific papers, we analysed 82 articles published by European researchers in 2014 and 2015. This review found that there is a general lack of information related to the optimal environmental conditions that should be provided for the range of species covered by this directive.

Group housing of gestating sows benefits their welfare by allowing them freedom of movement and the opportunity for social interaction. However, social life could also bring disadvantages for individuals who receive direct aggression or are displaced from the feeder. The aim of this study was to investigate associations between social behaviour, body condition and live weight. Gestating sows (n=298) were investigated on a commercial farm. Sows were housed in mixed parity groups where two single space, ad libitum trough feeders served 12 animals. Sows were weighed, body condition scored and had their back fat layer measured at mixing, 4 weeks after insemination and again before farrowing. Social status was estimated based on the numbers of won and lost agonistic interactions at mixing and at the end of gestation. In addition, tear staining was scored before the farrowing and reproductive performance data were collected. With the aid of video recordings, 100 to 150 interactions per group were observed. Winning percentage at mixing and at the end of gestation were associated (P<0.05) and appeared relatively stable within individuals. Tear staining scores and litter sizes were not associated with winning percentage at the end of gestation. However, live weight, relative weight, body condition and back fat thickness were associated with winning percentage (P<0.05), giving heavier animals an advantage. Low winning percentage related to lower live weight gain, probably due to poorer success in competition for feed. Live weight within a mixed parity group could be used as a proxy measure for social status. Sows with low body condition score and submissive sows might need special attention with regard to group dynamics and housing to alleviate the effects of competition in group housing.

There is community concern about the treatment of farm animals post-farm gate, particularly animal transport and slaughter. Relationships between lamb behavioural and physiological variables on farm, stockperson, dog and lamb behavioural variables pre-slaughter and plasma cortisol, glucose and lactate in lambs post-slaughter were studied in 400 lambs. The lambs were observed in three behavioural tests, novel arena, flight distance to a human and temperament tests, before transport for slaughter. Closed-circuit television video footage was used to record stockperson, dog and lamb behaviour immediately before slaughter. Blood samples for cortisol, glucose and lactate analyses were collected on farm following the three behavioural tests and immediately post-slaughter. The regression models that best predicted plasma cortisol, glucose and lactate concentrations post-slaughter included a mixture of stockperson and dog behavioural variables as well as lamb variables both on-farm and pre-slaughter. These regression models accounted for 33%, 34% and 44% of the variance in plasma cortisol, glucose and lactate concentrations post-slaughter, respectively. Some of the stockperson and dog behaviours pre-slaughter that were predictive of the stress and metabolic variables post-slaughter included the duration of negative stockperson behaviours such as fast locomotion and lifting/pulling lambs, and the duration of dog behaviours such as lunging and barking at the lamb, while some of the predictive lamb behaviour variables included the durations of jumping and fleeing. Some of the physiological and behavioural responses to the behavioural tests on farm were also predictive of the stress and metabolic variables post-slaughter. These relationships support the well-demonstrated effect of handling on fear and stress responses in livestock, and although not direct evidence of causal relationships, highlight the potential benefits of training stockpeople to reduce fear and stress in sheep at abattoirs.

Controlling rumen fermentation using buffering agents could contribute to enhancing ruminant productivity and performance. This study was realized to investigate the effect of dietary supplementation of AcidBuf, sodium bicarbonate, calseapowder and WMC seaweed (Utva Lactuca extra) on the animal performance, volatile fatty acids, rumen pH, rumen histology and carcass characteristics of growing male Awassi lambs. A total of 60 lambs was divided into five groups. One group served as a control and fed only on a concentrate diet without any buffering, whereas the other four groups were fed the concentrate diet supplemented with 0.4% AcidBuf (Buf1), 0.4% AcidBuf plus sodium bicarbonate, 50 : 50 (Buf2), 0.4% calseapowder (Buf3) or 0.4% WMC Seaweed (Buf4) for 98 days. The feed conversion ratio was (P<0.05) improved in Buf2 compared to the control and other treatment groups. The propionic acid decreased, whereas butyric acid was increased in the treatment groups (P<0.05) compared to the control. The pH of the rumen fluid and the length of submucosa were (P<0.05) higher in Buf4 and Buf1, respectively, compared to the control. Hot and cold carcass weights were (P<0.05) higher in Buf4 compared to Buf1. Lean meat percentage and rib eye area were (P<0.05) higher in Buf4; while the fat percentage was (P<0.05) lower in Buf2 and Buf4 groups compared to the control. The lightness and yellowness of meat were (P<0.05) higher in Buf1 and Buf4 compared to the control. The meat pH was (P<0.05) higher in Buf3 and Buf4 compared to Buf2 (at 1 h) and control (at 24 h). The visceral depot fat (%) was reduced with Buf3 and Buf4 compared to the control. The results indicated that dietary supplementation of different buffering agents improved feed efficiency, rumen pH, carcass characteristics and decreased the body fat in growing Awassi lambs.

Surplus accounting is a method for evaluating trends in how a firm’s productivity factors (intermediate inputs, capital, land, labour) are performing and how the productivity gains are redistributed between agents in the economy. Here the surplus accounting method was applied on a database of 164 Charolais-area suckler cattle farms running from 1980 to 2015. Over this 36-year period – with differences per sub-period – the cumulative productivity surplus (PS) increased at a low rate of +0.17%/year (i.e. cumulative volume of outputs produced increased slightly more than cumulative volume of inputs used). This timid increase in PS is linked to the constant expansion in labour productivity whereas other factor productivities have shrunk. The observable period-wide macrotrends are that commercial farm businesses struggle to protect their revenues, we also observe a slight fall in input prices, land rent and financing costs, and a huge climb in direct support-policy payments. The bulk of the cumulative economic surplus has been captured downstream – 64% downstream of the cattle value chain as a drop in prices, and 22% downstream of other value chains (chiefly cereals). It emerges that the productivity gains in beef cattle farming mostly benefit the downstream value chain (packers–processors, distributors and consumers), whereas it is mainly government money backing this drop in prices of agricultural output. Here we see the principal of the 1992 ‘MacSharry’ reform at work, with a transfer from the taxpayer through direct support-policy payments through to the consumer via lower prices. The simple fact that farmers’ incomes are stagnating is a clear indication that they are net losers in this distribution of productivity gains, despite the improvement in labour factor productivity.

Vitamins play an essential role in broiler nutrition. They are fundamental for normal metabolic and physiological process, and their requirements for poultry are not fixed and can be affected by multiple factors. In contrast, mycotoxins are a challenging issue because they hinder performance and the immune system. Vitamin supplementation above minimum requirements would permit improvement in productive potential, health, bone and meat quality in a situation of mycotoxin challenge. The objective of this study was to determine the influence of optimum vitamin nutrition in diets contaminated with aflatoxin in broilers from 1 to 44 days of age. A total of 1800 Cobb 500 male chicks were randomized to 15 sets of eight treatment groups, each containing 15 birds using a 2 × 2 × 2 factorial design (commercial vitamin levels and high vitamin levels, two levels of aflatoxin – 0 and 0.5 ppm with binder levels of 0 and 10 000 mg/kg). The mash diets were corn and soybean meal based, formulated according to commercial practices. Feed intake, weight gain and feed conversion were analyzed for birds from 1 to 44 days of age. To determine carcass characteristics (carcass yield, breast yield and leg yield) and black bone syndrome, two birds were slaughtered from each group at 45 days. Other analyses included breast tenderness, water loss by dripping and malonaldehyde concentrations. The results demonstrated that broilers that were fed high levels of vitamins showed better weight gain, feed conversion, carcass yield and breast yield than broilers that were fed diets with commercial vitamin levels (P < 0.05); also, broilers that were fed diets containing 0.5 ppm aflatoxin had lower weight gain, carcass yield and breast yield (P < 0.05). The use of 10 000 mg/kg of binder improved (P < 0.05) feed conversion throughout the rearing period. We conclude that aflatoxin negatively affects performance and carcass yield; however, feeding optimum vitamin nutrition improved these performance traits.

Insight into current scientific applications of Big Data in the precision dairy farming area may help us to understand the inflated expectations around Big Data. The objective of this invited review paper is to give that scientific background and determine whether Big Data has overcome the peak of inflated expectations. A conceptual model was created, and a literature search in Scopus resulted in 1442 scientific peer reviewed papers. After thorough screening on relevance and classification by the authors, 142 papers remained for further analysis. The area of precision dairy farming (with classes in the primary chain (dairy farm, feed, breed, health, food, retail, consumer) and levels for object of interest (animal, farm, network)), the Big Data-V area (with categories on Volume, Velocity, Variety and other V’s) and the data analytics area (with categories in analysis methods (supervised learning, unsupervised learning, semi-supervised classification, reinforcement learning) and data characteristics (time-series, streaming, sequence, graph, spatial, multimedia)) were analysed. The animal sublevel, with 83% of the papers, exceeds the farm sublevel and network sublevel. Within the animal sublevel, topics within the dairy farm level prevailed with 58% over the health level (33%). Within the Big Data category, the Volume category was most favoured with 59% of the papers, followed by 37% of papers that included the Variety category. None of the papers included the Velocity category. Supervised learning, representing 87% of the papers, exceeds unsupervised learning (12%). Within supervised learning, 64% of the papers dealt with classification issues and exceeds the regression methods (36%). Time-series were used in 61% of the papers and were mostly dealing with animal-based farm data. Multimedia data appeared in a greater number of recent papers. Based on these results, it can be concluded that Big Data is a relevant topic of research within the precision dairy farming area, but that the full potential of Big Data in this precision dairy farming area is not utilised yet. However, the present authors expect the full potential of Big Data, within the precision dairy farming area, will be reached when multiple Big Data characteristics (Volume, Variety and other V’s) and sources (animal, groups, farms and chain parts) are used simultaneously, adding value to operational and strategic decision.

There is currently a lack of research investigating the effectiveness of commercial broiler enrichments, and in particular the ability of these additions to create opportunities for positive welfare. One aim of this study was to investigate whether offering broiler chickens enrichments that have recently been found to be preferred leads to increased levels of activity. A second aim was to investigate the emotional effects of provision of these enrichments by assessing levels of fearfulness and play-like activity. Commercially housed broilers were assigned to treatment houses containing either: (1) platform perches, (2) platform perches+peat dust baths, (3) no enrichment (control). Activity levels and play behaviours in unenriched areas of the house were measured in weeks 3, 4 and 5. Levels of active behaviours, such as foraging and locomotion, were determined from video recordings of undisturbed birds in unenriched areas of the house. To stimulate play-like behaviours an observer walked through the birds, displacing them and creating a space. The broilers using the space were then filmed for 5 min and the occurrences of frolicking, sparring and food-running were recorded. Fearfulness of broilers in unenriched areas of the house, and also when using enrichments, was measured using observer avoidance tests in week 5. We found that creating space among the broilers was a successful method of stimulating play (largely sparring and frolicking), with play being observed in 93% of videos, however the presence of enrichments did not have an effect on the level of play recorded (P>0.05). There was also no treatment effect on activity levels of broilers in unenriched areas (P>0.05), however levels of overall activity decreased as broilers aged. Compared with the control, flight distances in unenriched areas were significantly lower in the perches+dust bath treatment (P=0.026), and were numerically lower in the perches treatment. This suggests a reduction in fearfulness with increased environmental complexity, and thus possible welfare benefits. It is suggested that further research should investigate whether increasing the level of provision of these enrichments leads to more marked improvements in welfare.

While group housing (GH) is mandatory in the European Union for the greater part of pregnancy, single housing in farrowing crates (FCs) during lactation that restrict sows in most of their natural behaviour patterns is still practised on a large scale. Research is urgently needed to develop alternative farrowing systems that improve sows’ welfare. Therefore, sows in three different farrowing systems – pens with FC, loose housing (LH) pens and GH for six sows – were compared regarding the level of skin injuries and their active and resting behaviour. A skin injury score was assessed for 15 body parts of 102 sows in six batches on 3 days (days 1, 14 and 34). In total, the active and resting behaviour of 77 sows in six batches was examined on 3 days (days 18, 25 and 32) between 0700 h and 1900 h by means of a scan sampling method. The suckling behaviour and the level of cross-suckling were analysed in GH by means of direct observation in four batches during three 4-h sampling periods (days 17, 24 and 31). No significant differences were found in total skin injuries when the sows entered the systems (day 1), but GH sows showed significantly higher total skin injuries compared to FC and LH sows in the middle (day 14) and at the end (day 34) of the lactation period. A significant difference between FC and LH sows was never seen. Differences were found for the proportion of different body postures between the three systems. The odds for lying in lateral recumbency versus standing and sitting versus standing were significantly higher for FC and LH sows compared to GH sows. Additionally, sows were significantly more likely to be standing as opposed to lying in lateral recumbency as the lactation period progressed. Cross-suckling was a frequent behaviour in GH, seen in 35.0% of all successful suckling bouts. However, only an average of 0.56 piglets per successful suckling bout was observed cross-suckling, suggesting only a few piglets were engaged in cross-suckling. In conclusion, the skin injury score was only moderately increased in GH compared to FC and LH and comparable to pregnant group-housed sows, both free farrowing systems seemed to be an environmental enrichment for lactating sows and good management cannot prevent the occurrence of cross-suckling in a GH system, but can probably reduce it.

Achieving a consistent level of robot utilisation throughout 24 h maximises automatic milking system (AMS) utilisation. However, levels of robot utilisation in the early morning hours are typically low, caused by the diurnal feeding behaviour of cows, limiting the inherent capacity and total production of pasture-based AMS. Our objective was to determine robot utilisation throughout 24 h by dairy cows, based on milking frequency (MF; milking events per animal per day) in a pasture-based AMS. Milking data were collected from January and February 2013 across 56 days, from a single herd of 186 animals (Bos taurus) utilising three Lely A3 robotic milking units, located in Tasmania, Australia. The dairy herd was categorised into three equal sized groups (n=62 per group) according to the cow’s mean daily MF over the duration of the study. Robot utilisation was characterised by an interaction (P< 0.001) between the three MF groups and time of day, with peak milking time for high MF cows within one h of a fresh pasture allocation becoming available, followed by the medium MF and low MF cows 2 and 4 h later, respectively. Cows in the high MF group also presented for milking between 2400 and 0600 h more frequently (77% of nights), compared to the medium MF group (57%) and low MF group (50%). This study has shown the formation of three distinct groups of cows within a herd, based on their MF levels. Further work is required to determine if this finding is replicated across other pasture-based AMS farms.

Lying behaviour is important for the welfare of the cow. Therefore, reliable electronic devices may improve the management of the cows, and the devices can be used as a tool in research. However, accelerometer-based devices measure acceleration, and an algorithm is therefore necessary for the calculation of lying behaviour. Thus, validation of such devices is imperative prior to use. The objective of this study was to validate the use of the AfiTagII device for measurements of the lying time and frequency of lying bouts of Danish Holstein (DH) and Danish Jersey (DJ) cows in a loose-house system on two different bedding materials. The validation included correlations and linear regression analyses of data collected by the AfiTagII compared with data collected both by direct observations and recordings from a previously validated device (IceQube). In total, 40 cows were observed directly with primiparous and multiparous DJ and DH cows, equally represented. Furthermore, 21 cows were monitored with both AfiTagII and IceQube devices, and data from both devices were collected simultaneously. The devices were attached to the hind leg of the cow. The estimates of the lying time from the AfiTagII device was highly correlated with the recordings from direct observations (r=0.98), and there was a linear relation between these with an intercept equal to 0 and a slope close to 1. The estimates of the lying time from the AfiTagII device was also highly correlated with the IceQube recordings (r=0.94). However, the intercept deviated from 0. The frequency of lying bouts recorded by the AfiTAgII compared to direct observations showed a positive predictive value of 0.96 for lactating cows on the slatted floor and of 0.85 for the dry cows on the deep bedding. The correlations between frequency of lying bouts recorded with the two devices were high (r=0.94), but the intercept deviated from 0. In conclusion, the AfiTagII has a high accuracy for the measurements of lying behaviour in both DH and DJ cows kept on different bedding materials.

Spineless cactus is a useful feed for various animal species in arid and semiarid regions due to its adaptability to dry and harsh soil, high efficiency of water use and carbohydrates storage. This meta-analysis was carried out to assess the effect of spineless cactus on animal performance, and develop and evaluate equations to predict dry matter intake (DMI) and average daily gain (ADG) in meat lambs. Equations for predicting DMI and ADG as a function of animal and diet characteristics were developed using data from eight experiments. The dataset was comprised of 40 treatment means from 289 meat lambs, in which cactus was included from 0 to 75% of the diet dry matter (DM). Accuracy and precision were evaluated by cross-validation using the mean square error of prediction (MSEP), which was decomposed into mean bias, systematic bias and random error; concordance correlation coefficient, which was decomposed into accuracy (Cb) and precision (ρ); and coefficient of determination (R2). In addition, the data set was used to evaluate the predicting accuracy and precision of the main lamb feeding systems (Agricultural and Food Research Council, Small Ruminant Nutritional System, National Research Council and Institut National de la Recherche Agronomique) and also two Brazilian studies. The DMI, CP intake (CPI), metabolizable energy (ME) intake and ADG increased when cactus was included up to 499 g/kg DM (P<0.001). In contrast, animals fed high levels of cactus (>500 g/kg DM) had a decreased DMI, CPI and NDF intake, but increased feed efficiency (P<0.001) and similar ADG compared with those without cactus addition. The DMI was positively correlated with initial BW, final BW, concentrate and ADG, while it was negatively correlated with cactus inclusion and ME of the diet. On other hand, ADG was positively correlated with DMI, initial and mean BW and concentrate, and it was negatively correlated with cactus inclusion. The two developed equations had high accuracy (Cb of 0.95 for DMI and 0.94 for ADG) and the random error of MSEP was 99% for both equations. The precision of both equations was moderate, with R2 values of 0.53 and 0.50 and ρ values of 0.73 and 0.71 for DMI and ADG, respectively. In conclusion, the developed equation to predict DMI had moderate precision and high accuracy, nonetheless, it was more efficient than those reported in the literature. The proposed equations can be a useful alternative to estimate intake and performance of lambs fed cactus.