The present study was designed to examine the pattern of changes in plasma leptin concentration during the early stage of young ruminants. Five crossbred lambs weaned at 45 days were housed individually in pens. The lambs were offered timothy hay, rolled barley and soya-bean meal to meet a 200 g daily gain, and were given free access to water and mineral block. Blood samples were collected just before the morning feeding and at 30-min intervals for 6 h after the morning feeding beginning at 0·5 months after weaning and thereafter monthly for the next 6 months. Plasma leptin level increased dramatically from 0·5 to 3 months after weaning. Thereafter, it dropped to a similar level to that at 2 months after weaning and remained at that level. Moreover, plasma leptin level was not affected by feeding, and no variation throughout the day of sampling was observed. Plasma insulin levels showed similarity to changes in plasma leptin levels. Plasma insulin levels were positively correlated (r = 0·63) with plasma leptin levels. This study shows that plasma leptin level dramatically increases after weaning. Additionally, it suggests that insulin contributes to the increase of leptin with growth in lambs.

Differences in receptor binding affinity for insulin, and insulin receptor concentration, were found in skeletal muscle and adipose tissue of lambs differing in genotype and gender. The Rouge de l’Ouest lamb genotype had a lower receptor affinity for insulin in m. rectus capitis skeletal muscle compared with both Dutch Texel and Greyface lamb genotypes (P < 0·01). Insulin receptor concentration in m. longissimus dorsi skeletal muscle was greater in the Texel lamb genotype compared with both the Greyface (P < 0·001) and Rouge de l’Ouest (P < 0·05) lamb genotypes. Insulin receptor concentration in perirenal fat tissue was greater in the Rouge de l’Ouest lamb genotype compared with the Texel lamb genotype (P < 0·05).

There were no differences in receptor affinity for insulin between individual tissues in male and female lambs. The concentration of insulin receptors in male lambs was greater in m. longissimus dorsi (P < 0·05), subcutaneous adipose tissue (P < 0·05) and perirenal fat (P < 0·001) compared with female lambs. Receptor affinity for insulin in individual tissue depots also demonstrated significant genotype ✕ gender interactions. M. longissimus dorsi had a lower receptor affinity for insulin in Texel male lambs compared with Greyface male lambs (P < 0·05). M. rectus capitis had a lower receptor affinity in Rouge de l’Ouest male lambs compared with Texel male lambs (P < 0·01). M. rectus capitis also had a lower receptor affinity for insulin in Rouge de l’Ouest female lambs compared with both Texel and Greyface female lambs (P < 0·01). Within individual genotypes, m. longissimus dorsi had a lower receptor affinity for insulin in Greyface female lambs compared with Greyface male lambs (P < 0·05) whereas m. rectus capitis had a lower receptor affinity for insulin in Texel female lambs compared with Texel male lambs (P < 0·05).

Such differences in tissue receptor affinity for insulin and receptor concentration in lambs differing in genotype and gender could, through effects on tissue sensitivity and/or responsiveness to insulin, manipulate nutrient partitioning between skeletal muscle and fat tissue and hence control, at least in part, the efficiency of lean meat production.

The objective of this study was to examine the effect of dietary vitamin A (retinol) on leptin in cattle. The relationship between plasma leptin and insulin was also examined. The experiment studies 16 9-month-old steers over the following 20-month fattening period. Four treatment diets were given to four steers in each of four groups. Cattle in treatment A were given a diet high in vitamin A (9000 to 10500 µg/day) throughout the experiment. Cattle in treatment B were given a diet low in vitamin A (300 to 4500 µg/day) for 0 to 10 months, after that they were given the high vitamin A diet during the period 10 to 20 months. Cattle in treatment C were given the low vitamin A diet for 0 to 14 months, after that they were given the high vitamin A diet for the period 14 to 20 months. Cattle in treatment D were given the low vitamin A diet throughout the experiment. Plasma samples were collected at 2-month intervals during the experiment to determine the plasma leptin, insulin and vitamin A concentrations. Plasma leptin concentration significantly increased over the fattening period (P < 0·001) but did not differ between the treatments (P > 0·05). Plasma leptin concentration was positively correlated with insulin concentration (r = 0·44, P < 0·001), although it was not correlated with plasma vitamin A concentration (r = –0·01, P > 0·05). It was concluded that dietary vitamin A in cattle does not affect plasma leptin concentration.

Studies in rodents and primates strongly indicate that prenatal stress affects the survival, behaviour and physiology of the offspring. Stressful stimuli during gestation may have a direct or hormone mediated effect on the development of stress systems in the foetal organism, resulting in an altered coping during stressful situations. The present study was conducted to elucidate prenatal stress effects in domestic pigs on the responses of the hypothalamicpituitary- adrenocortical (HPA) axis and the sympatho-adrenomedullary (SAM) system as well as on morbidity, mortality and growth of the offspring. Pregnant sows were subjected to a restraint stress for five minutes daily during the last five weeks of gestation. Endocrine reactions of the piglets were tested at 3, 7, 21 and 35 days of age using an immobilization test and an ACTH challenge test. Prenatally stressed piglets showed lower basal plasma cortisol and increased corticosteroid binding globulin (CBG) concentrations at 3 days of age, indicating decreased free cortisol concentrations after birth. Cortisol levels after ACTH stimulation and catecholamine levels after immobilization were not affected by the stress treatment of the sows. Piglets from stressed sows tended to have lower noradrenaline : adrenaline ratios at three days of age compared with the control piglets. In addition, stressed sows tended to have lower litter weights after birth. The morbidity and mortality during the suckling period was higher in the prenatally stressed litters, as shown by a higher frequency of diseased and perished piglets per litter. We suppose that prenatal stress during late gestation in pigs alters the development of the HPA system and impairs the vitality of the offspring.

This study was designed to explore the effect of protein source on muscle susceptibility to lipid peroxidation in two representative species of fish farmed for human consumption: the freshwater rainbow trout and the seawater European sea bass. Four isoproteic diets (digestible protein in the range 366 to 392 for rainbow trout and 391 to 415 g/kg for European sea bass) were formulated to contain one of the following as the main protein source: fish meal, warm water alcohol-extracted or toasted soya protein concentrates or maize gluten meal. Highest daily growth indices were always achieved using the diets based on fish meal as the main source of protein (P < 0·05). Fish of both species given diets containing maize gluten and the toasted soya protein concentrate showed slowest growth. The depressant growth effect of the vegetable protein concentrates was greater in sea bass than in rainbow trout. Dietary treatment was not correlated with any significant effect on whole-body composition or intramuscular fat content except for ash concentration in European sea bass. Under conditions of forced peroxidation in vitro for 240 min, muscle specimens of trout and sea bass given diets containing fish protein as the main source of protein showed the highest peroxidation levels (P < 0·05); while the lowest peroxidation values were found in fish given maize gluten-containing diets (P < 0·05). In the present case, the partial substitution of fish meal with vegetable proteins in diets led to a lower susceptibility of fish flesh to peroxidation. This finding may have applications in the production of fish of improved quality and longer shelf life.

Three weaning treatments (weaning at 45 days of age, at 65 days of age, unweaned) and two slaughter weights (24 and 28 kg) were compared in lambs raised at pasture, and their effect on carcass and meat traits were studied. Unweaned lambs displayed a greater growth rate, lower concentrate consumption, greater dorsal fat thickness, more kidney knob and channel fat and greater total fat levels, measured through dissection of the hind limb. Total collagen concentration was lower in unweaned lambs and the intramuscular fat of these same animals exhibited a greater proportion of saturated fatty acids and a lower n-6/n-3 fatty acid ratio. Slaughter weight influenced scored carcass fatness, which was greater in heavier lambs, meat colour, which displayed greater lightness in lower-weight lambs, and water-holding capacity, which was lower in lambs with the lower slaughter weight. The heavier lambs displayed lower soluble collagen values, and the intramuscular fat of the longissimus dorsi muscle of these same lambs contained higher levels of monounsaturated and lower levels of polyunsaturated fatty acids.

A 3-week feeding trial using 300 1-day-old male broiler chicks was conducted to evaluate the effects of adding three levels of a microbial phytase (Finnfeed phytase; 250, 500 and 1000 U per kg diet) to a maize-soya-bean-meal diet containing 3·0 g/kg non-phytate phosphorus and 8·0 g/kg calcium. A diet containing 4·0 g/kg non-phytate phosphorus and 9·0 g/kg calcium served as the positive control. The responses were evaluated in terms of broiler performance, toe ash contents, ileal phytate degradation, ileal digestibility of nitrogen, amino acids, phosphorus, starch and fat, apparent metabolizable energy, apparent ileal digestible energy, and apparent retention of phosphorus and nitrogen. The addition of 500 U phytase per kg diet to the phosphorus-deficient maize-soya-bean-meal diet improved the performance and toe ash contents of broilers to equal those given the positive control diet. Ileal phytate degradation data provided direct evidence to the efficacy of phytase in hydrolysing the phytic acid. Addition of 500 U phytase per kg to the maize-soya-bean-meal diet which contained 3·0 g phytate-phosphorus per kg resulted in more than doubling of phytate degradation from 0·218 to 0·481. Supplemental phytase improved ileal digestibility of nitrogen, amino acids, starch and lipids, with these improvements being eventually reflected in enhancements in ileal digestible energy and apparent metabolizable energy.

To investigate the utilization of different carbohydrate sources and the possible protein-sparing effects of carbohydrates for grouper (Epinephelus malabaricus), six isoeneregtic purified diets were prepared. Three dietary protein levels (500, 460, 420 g/kg) were achieved by substitution with three levels (143, 195, 246 g/kg) and two sources (glucose and starch) of dietary carbohydrate. Each of the six diets was given to triplicate groups of grouper in a recirculating rearing system for 8 weeks. In the glucose group, weight gain of fish decreased with increasing dietary glucose and decreasing protein content. In the starch group, weight gain of fish given the 500 g/kg protein diet was higher (P < 0 ·05) than fish given the 460 and 420 g/kg protein diets. At each of the dietary protein levels weight gain of the glucose-group fish and the starch-group fish were similar (P > 0·05). At the 500 g/kg dietary protein level body lipid content of the starch-group fish was higher than that of glucose-group fish. At 500 and 460 g/kg dietary protein levels, fish given starch diets had higher hepatic hexokinase activity than fish given the glucose diets. These results suggest that, at the levels studies, the utilization of starch and glucose by grouper is similar. Decreasing the dietary protein level from 460 g/kg to 420 g/kg by increasing the starch content in the diet from 195 g/kg to 246 g/kg did not reduce (P > 0·05) weight gain and food efficiency, suggesting that starch could spare some protein when the dietary protein level is low.

The aim of this study was to evaluate the performance and digestive response of the growing rabbit according to a change in the nature of the lignocellulose (ADF), without a change in the ADF level. Three diets with a linearly decreasing lignin/cellulose ratio (ADL/cellulose = 0·8; 0·4; 0·2; respectively LC8, LC4, LC2) were given to a total of 162 rabbits per diet, from weaning till slaughter weight. A reduction of the ADL/cellulose ratio led to a lower food intake (LC8 v LC2: -11%), associated with a lower daily weight gain (-6% for LC2). Mortality and morbidity rate was significantly greater (+50%) for LC2 and LC4 diets compared with the LC8. Thus, the health risk index (morbidity + mortality) increased by 20 units for LC4 and LC2 compared with LC8 diet. In parallel, rate of passage was slower (mean retention time 4·5 h greater, P = 0045) with LC4 and LC2 diets. Dry-matter digestibility was slightly affected, whereas protein digestion was favoured with low ADL/cellulose ratio. It is recommended that a minimum lignin supply (about 6 g/day) is adopted as well as a minimum dietary lignocellulose to ensure growth performance and health of the growing rabbit.

The research was carried out to evaluate the effect of different dietary α-tocopheryl acetate (DTA) concentrations in light-weight lambs on muscle α-tocopherol accumulation and on quality characteristics of stored meats. Thirty-two Manchego lambs were randomly distributed to four groups and given diets containing four levels of DTA (20, 270, 520 and 1020 mg/kg diet) for 6 weeks. Lambs were slaughtered at live weights ranging from 23·5 to 26·4 kg. A linear (P < 0·001) and quadratic (P < 0·001) effect of dietary supplementation level was observed on muscular α-tocopherol concentration, which fitted the following equation: mg muscle α-tocopherol per kg muscle = 1·78(s.e. 0·18) + 7·08 (s.e.0·89)(1 - e00012DTA)) (P < 0·001, R2 = 0·99). There was a linear effect (P < 0·001) of muscle vitamin E concentration on thiobarbituric acid reactive substance on day 0 of storage, but a linear plus quadratic effect (P < 0·001) on days 3, 6 and 9. Broken line analysis of data at day 9 of storage indicated a target muscle α-tocopherol concentration of 5·4 mg/kg. Evolution of surface redness of lamb chops also showed a linear and quadratic effect of dietary treatment on days 3 and 6 of storage, but only a linear effect on day 9. Broken line analysis of data at 3 and 6 days indicated a target α-tocopherol concentration in the range 5·3 to 5·6 mg/kg muscle for optimum red colour stability. Surface luminosity showed no effect of dietary treatment at days 0, 3 and 6 of storage but a linear (P < 0·01) plus quadratic (P < 0·05) effect on day 9 of storage. Broken line analysis at this point indicated a target muscle α-tocopherol concentration of 3·2 mg/kg. It is concluded that the effectiveness of dietary α-tocopheryl acetate supplementation depends on the meat quality attribute assessed. A significant positive effect for lipid oxidation can be reached even at the lower supplementation level utilized in this experiment (270 mg/kg diet). However, considering the protecting effect at different storage times and particularly the effect on meat surface redness, the optimum level would be in the range 5·3 to 5·6 mg/kg muscle, which correspond to a dietary inclusion of 550 to 625 mg α-tocopheryl acetate/kg diet.

An experiment was conducted to measure the effects of stocking density (increased number of pigs per pen) on lysine requirements of pigs grown from 25 to 60 kg live weight. Two hundred and sixty-four female Large White ✕ Landrace pigs were assigned at 25 kg to one of four dietary lysine treatments (13·3 (L1); 11·4 (L2); 9·5 (L3) and 7·6 (L4) g/kg) and either seven or 13 pigs per pen (or 1 0 and 0·5 m2 per pig, respectively). An additional treatment of one pig per pen (20 m2 per pig) was included to compare the responses of solitary- versus group-penned pigs. Animals were given ad libitum access to dietary treatments from a mean pen starting weight of 261 (s.e. 0·35) kg to a mean pen finishing weight of 63·4 (s.e. 0·61) kg live weight. There were no significant interactions between dietary lysine content and floor space per pig on food intake (FI), average daily growth rate (ADG), the amount of food per unit of gain (FCR) and the rate of protein retention (PR). Significant interactions were evident for body composition and the rate of lipid retention (LR). Over the weight range 25 to 40 kg there were significant differences in FI (P < 0·05) and FCR (P < 0·001) between dietary lysine treatments but most of these differences had disappeared over the 40 to 60 kg live weight. Individually penned animals had significantly higher (P < 0·05) FI and ADG than group-penned animals. However, there were no differences between seven and 13 pig per pen treatments. Stocking density had no effect on LR or body protein content but did cause a significant reduction in PR (P < 0·001) and an increase in body lipid content (P < 0·05) as the number of pigs per pen increased from seven to 13. Lysine requirements (expressed in g/day) therefore could be seen to be reduced with increasing stocking density. However, as lysine intake was reduced in group-penned animals, the reduced daily requirement does not necessarily warrant a reduction in the lysine content of the food. Feeding according to the requirements for maximum PR will still produce the best carcass and growth performance irrespective of the group size. The improvement in PR associated with higher dietary nutrient levels did not completely offset the adverse physiological effects of higher stocking density but may partly counteract the effect of reduced lysine intake. However, there were indications that feeding crowded pigs a lower dietary lysine concentration may not further reduce the already diminished protein (lysine) growth rate. An additional experiment was performed to test whether the number of feeder bins may have constrained food intake and therefore growth in group-penned animals. The results of this experiment showed that the number of bins had no significant effect on FI, ADG and FCR in group-penned pigs, and therefore a single feeder bin was not considered a constraining factor in pigs housed with limited floor space.

Many studies with fin fish have demonstrated the potential to use alternative dietary protein sources to fish meal based on growth responses, although these trials mostly neglect to determine if such protein sources affect immune function. This study investigated the effect of fish meal replacement with dehulled lupin meal (LPN) or hydrolysed poultry feather meal (FTH). Atlantic salmon (Salmo salar L.) parr were supplied isonitrogenous and isoenergetic diets with 40% of the dietary protein provided by LPN or FTH, or 400 g/kg of the dietary protein provided equally by LPN and FTH (MIX). A diet mainly containing fish-meal protein acted as a control (CON). Growth, immune function, blood chemistry and disease resistance were assessed after 56 days. Significant differences (P < 0·05) in weight gain were detected between Atlantic salmon given the CON and FTH diets, whilst those salmon given LPN and MIX did not differ from any other. Productive protein values were significantly lower (P < 0·01) for salmon on FTH compared with those on CON and MIX. Immune function (as assessed by lysozyme, antiprotease, neutrophil oxygen radical production and plasma total immunoglobulin) and blood chemistry (as assessed by plasma total protein and glucose) were not significantly (P > 0·05) affected by any diet. Mortality rates of Atlantic salmon challenged with Vibrio anguillarum were not influenced by diet. These data suggest Atlantic salmon could be supplied diets with the fish meal component reduced to supply approximately 600 g/kg of the total protein, with the remaining 400 g/kg supplied by dehulled lupin meal or a combined dehulled lupin and hydrolysed poultry feather meal without any adverse effects on growth, immune function or blood chemistry.

Young male red deer follow a seasonal growth pattern that can be shifted by altering the photoperiod they experience. An increase in photoperiod to 16 h of light per day (16L : 8D) during winter advances the onset of rapid growth and high food intake that normally commences in spring. These changes are associated with increased growth hormone (GH) and insulin-like growth factor-1 (IGF-1) secretion. The GH/IGF-1 axis is acutely sensitive to the level of nutrition and the relative rôles of photoperiod and nutrition in determining the spring IGF-1 rise is unknown. The present experiment set out to examine this by exposing two groups of deer (no. = 8 per group) to a photoperiod shift during their 1st year of life (16L : 8D from 2 June), designed to cause accelerated growth and increased food intake after approximately 7 weeks. However, after 6 weeks the food intake (pellets containing 11 MJ metabolizable energy and 160 g crude protein per kg dry matter (DM)) of one group (LDRES) was clamped, thereby preventing the intake component of the response. The intake of the other group (LDAL) remained ad libitum for a further 12 weeks until 6 October, when the experiment concluded.

During the first 6 weeks of 16L : 8D, growth rate (118 (s.e. 15·4) g/day) and food intake (1·37 (s.e. 0·031) kg DM per head per day) did not differ between the groups. Food intake following the clamp in LDRES averaged 1·40 (s.e. 0·015) kg per head per day. The intake of LDAL increased 2 weeks after the clamp and thereafter was higher than LDRES (P < 0·001). Food intake of LDAL averaged 2·13 (s.e. 0·051) kg during the nutritional clamp period. Growth rates increased in both groups during the first 3 weeks of the clamp, averaging 237 (s.e. 25·0) g/day, then growth slowed in LDRES and live weights diverged. Growth rates until the end of the experiment (147 (s.e.23·0) g/ day v. 299 (s.e. 12·5) g/day, P < 0·001) and mean live weight over the last 5 weeks of the experiment were lower (P < 0·05) in LDRES than LDAL, weights reaching 88·3 (s.e. 1·86) kg and 97·9 (s.e. 2·74) kg respectively on the final sampling date. Metatarsal bone length grew more in LDAL than in LDRES (3·1 v. 2·2 cm, s.e.d. = 0·23, P < 0·01). Prior to the nutritional clamp, mean plasma prolactin and IGF-1 concentrations increased at 3 and 6 weeks after 16L : 8D respectively, in both groups. Prolactin concentrations were lower in LDRES than LDAL on two occasions, at weeks 3 and 7 after the onset of the nutritional clamp, and IGF-1 concentrations were lower in LDRES than LDAL (676 v. 872 ng/ml, s.e.d. = 73·8, P < 0·05) over the last 7 weeks of sampling.

In summary, a photoperiodically driven increase in IGF-1 occurred even when the usual associated increase in food intake was prevented. This indicates that the seasonal IGF-1 rise in red deer is not a consequence of the increased food intake, although the latter appears necessary to maintain elevated IGF-1 concentrations. The rise in IGF-1 may therefore be considered as a component of the photoperiodically entrained seasonal drive to grow, and the increase in food intake a response to satisfy the increased energy demand.

A comparison of in vitro microbial activity was made between inocula from faeces of adult and unweaned pigs, using a range of carbohydrate-rich substrates. The substrates tested were classified into groups (fibre-rich, grains, gums, pectin, saccharides, storage carbohydrates, and miscellaneous). Proximate and fibre analyses were determined for all substrates tested. Fermentation kinetics were measured according to the cumulative gas production technique. End-point products such as gas, volatile fatty acids and ammonia were also determined. The faeces from the adult pigs (ATF) were collected from four castrated finisher pigs (Dutch Landrace × Great Yorkshire). These were offered a diet based on highly digestible maize starch and fish meal, so as to be as free as possible of fermentable carbohydrates. The unweaned piglet faeces were collected from 19 unweaned piglets (male and female) which were 27 to 30 days old. The parameters were tested for significance by an analysis of variance using the Tukey's studentized range test of multiple comparisons. There were significant differences in the fermentation patterns both due to source of substrate and inoculum. It would appear that the metabolic activity of the microflora does differ significantly between adult and weanling pigs, though this varied for the different carbohydrates tested. Suggestions are made as to how to select potential carbohydrate ingredients for an in vivo experiment, based on their in vitro fermentation characteristics.

The effect of recombinant bovine FSH (rbFSH) administration on serum FSH and follicular growth was determined in 16 GnRH-immunized heifers (primary day 0 and booster day 28 using 0·4 mg human serum albumin (HSA) – Cys-Gly-GnRH in DEAE-dextran). All heifers produced GnRH antibody titres (mean 58·8 (s.e. 2·8)% binding at 1: 640 serum dilution on day 42), follicular growth ceased (follicles ≤ 4 mm) between days 33 and 49, progesterone concentrations were ≤ 0·5 ng/ml in all heifers by day 53, and pulsatile LH secretion was markedly reduced (v. luteal phase control heifers) on day 63. Heifers were blocked by antibody titre and LH concentrations on day 63 and assigned (no. = five or six per treatment) on day 78 to: (1) control (saline given i.m. four times per day for 4 days), (2) 0·5 mg equivalent (USDA bFSH BP 1) of rbFSH (Granada Biosciences, TX) given i.m. four times per day for 6 days (12 mg in total) or 3) 1·5 mg rbFSH given i.m. four times per day for 4 days (24 mg in total). Ovaries of heifers were examined by ultrasound and blood samples were collected at 3- to 6-h intervals from days –1 to 10 and then twice a day until follicles ≥ 5 mm were no longer detectable. The increase in serum FSH concentrations was different (P < 0·05) between treatments (mean peak FSH above pre-treatment baseline concentrations at 102 h after first rbFSH were 1·4 (s.e. 0·8), 14·5 (s.e. 3·3) and 33·6 (s.e. 5·3) ng/ml for treatments 1 to 3, respectively). The rate of decline in FSH from 102 to 302 h was not different between treatments 2 and 3. There was no detectable follicular growth in heifers given saline injections. Following either rbFSH treatment, medium (MF; 5 to 9 mm) and large (LF; ≥ 10 mm) follicles grew. For heifers in treatments 2 and 3, there was no difference in the interval from start of rbFSH until the first appearance of MF (4·2 (s.e. 0·7) v. 3·3 (s.e. 0·3) days) or LF (5·3 (s.e. 0·3) v. 4·8 (s.e. 0·8) days) or in the duration of the presence of MF and/or LF (13·2 (s.e. 4·5) v. 16·6 (s.e. 2·6) days). Increasing dose of rbFSH increased (P < 0·05) the mean maximum number of MF (4·3 (s.e. 1·1) and 9·2 (s.e.0·9) for treatments 2 and 3) and LF (2·0 (s.e. 1·1) and 8·4 (s.e. 1·2)). In summary, low doses of rbFSH induced a small cohort of follicles to grow and develop without selection or development of a single dominant follicle, whereas high doses of rbFSH resulted in large numbers of both MF and LF.

When weaned early, piglets commonly take some time to accept solid food, resulting in a growth check and reduced welfare. The transmission of information about food between animals has been demonstrated in other species and it would be advantageous if this occurred in piglets. This experiment investigated the effects of pairing piglets that were consuming solid food with newly weaned piglets. Six litters of piglets did not receive solid food until weaning. In each litter four piglets (3 plus 1 spare) were weaned at 21 days of age and housed together for 7 days and offered one of two foods (3 litters per food). At 28 days of age the remaining piglets were weaned and four pairs of piglets were formed, such that there were three experienced animals paired with three inexperienced observers, each pair having visual contact and varying degrees of physical contact (1: none, 2: through wire mesh, 3: housed together), and a pair of inexperienced piglets (4: housed together) to act as controls. Food intake and weight gain were recorded over a period of 7 days. There was no effect of food type on food intake or live-weight gain of the pairs but the inexperienced pigs had higher gains on food 1 than food 2. The inexperienced pairs ate less food than the other pairs and the experienced/observer pairs that were housed together had the greatest weight gain. The level of variation between piglets was such that there were no significant effects of pairing treatment on the weight gain of the inexperienced animals. Total time spent feeding increased with time from pair formation. The number of simultaneous feeding events was higher for the experienced/observer pairs housed together than for the inexperienced pairs. This experiment has indicated that food intake is stimulated when an inexperienced piglet is housed with an experienced piglet and, with further work, this could be exploited to alleviate the weaning check.

Two experiments with lambs given food indoors and individually penned were designed to study the effects of different levels of ground maize and citrus pulp as supplements of a diet based on urea-treated straw (5 kg urea per 100 kg straw) offered ad libitum over a period of 16 weeks (experiment 1) or 10 weeks (experiment 2). The voluntary intake, live-weight gain (LWG), organic matter digestibility (OMD), urinary allantoin-nitrogen (UAN) excretion and acetate clearance rate were measured. The lambs were blocked on weight and randomly assigned to the treatments described below. Ruminal outflow rate of the solid and liquid phases from the rumen were also measured in experiment 2.

In experiment 1, 20 female lambs from the Ile-de-France breed, with an initial live weight (LW) of 43 (s.e. 3·3) kg were used. Wheat straw (WS) was supplemented with 50 g/kg of fish meal (FM) and with 0, 100, 200 or 300 g/kg of ground maize on a dry-matter (DM) basis (M0, M1, M2 and M3, respectively). In experiment 2, 25 female lambs from the Portuguese breed Churra-da-Terra-Quente, with an initial LW of 24·2 (s.e. 4·3) kg were used. The straw was offered ad libitum during 10 weeks and supplemented with 50 g/kg of FM and 0, 100, 200, 300, or 400 g/kg of dried citrus pulp on a DM basis (CP0, CP1, CP2, CP3 and CP4, respectively).

During the experiments, all animals were moved to metabolism cages to measure OMD and UAN excretion. Two additional incubation studies were carried out with rumen fistulated rams (experiment 1) or cows (experiment 2) given the diets described above close to the maintenance feeding level.

In experiment 1 daily straw DM intake linearly decreased (P < 0·05) from 21·6 to 17·7 g/kg LW and LWG linearly increased (P < 0·05) from 51 to 154 g/day for treatments M0, M1, M2 and M3, respectively. The rate of straw DM degradation was significantly decreased (P < 0·01) by maize supplementation. Straw OMD (kg/kg) was 0·562, 0·583, 0·547 and 0·520 and UAN (mg/day) was 620, 790, 854 and 859 for treatments M0, M1, M2 and M3, respectively. Acetate clearance rate, increased (P < 0·05) as the level of maize inclusion increased.

In experiment 2 daily straw DM intake was 23·3, 25·8, 24·7, 23·5 and 18·6 g/kg LW per day and LWG was –9, 28, 44, 64 and 67 g/day for treatments CP0, CP1, CP2, CP3 and CP4, respectively. Supplementation significantly increased LWG (P < 0·001) but at the 400 g/kg level depressed straw DM intake. Straw OMD linearly decreased (P < 0·05) from 0·484 (CP0) to 0·428 (CP4) g/kg and UAN (mg/day) was 181, 303, 363, 384 and 392 for treatments CP0, CP1, CP2, CP3 and CP4, respectively. Rumen outflow rate of fibre particles was unaffected by supplementation while the outflow of liquid phase tended to be increased (P < 0·10). The rate of DM degradation was significantly reduced (P < 0·01) by citrus-pulp inclusion. Acetate clearance rate was unaffected (P > 0·05) by citrus-pulp supplementation.

The results of these experiments demonstrate that supplementation of urea-treated straw with ground maize up to 200 g/kg or with citrus pulp up to 300 g/kg of the diet DM increased or did not depress straw intake, increased the supply of microbial protein and have no significant effect on straw digestibility. The efficiency of utilization of absorbed energy was apparently improved by maize but not by citrus-pulp supplementation.

Seventy-two entire male pigs (initially 28 kg body weight) were used to study the effects of by-product (pollard, rapeseed meal, peas and copra meal combined) inclusion level with or without expander processing, on growth performance and nutrient digestibility in a 3 ✕ 2 factorial arrangement of treatments. Productive performance and nutrient digestibility coefficients (no. = 6) were determined in individually fed pigs offered pelleted diets ad libitum containing a low by-product diet (350 g/kg), medium by-product diet (520 g/kg) and high by-product diet (752 g/ kg). The expanded diets were processed at 105ºC for 5 s at 35 bar pressure having been previously conditioned at 85ºC for 5 s. All diets were formulated using standard values for ingredients to have similar concentrations of digestible energy (DE) and ideal protein. Starch contents of 437, 401 and 314 g/kg and crude fibre contents of 48, 58 and 74 g/kg were recorded for the low, medium and high by-product diets respectively. No by-product level ✕ expander processing interactions (P > 0·05) were observed in growth criteria, carcass characteristics or nutrient digestibility. Expander processing decreased (P < 0·05) the apparent organic matter (OM), ash and energy digestibility of the diets, as well as the digestible energy content of the diets. The level of by-product in the diet had no statistically significant effect on the OM, protein and energy digestibility or the DE content of the diets. The increase in by-product inclusion levels decreased (P < 0·05) average daily gain (ADG) (0·899 v. 0·888 v. 0·854 (s.e. 0·014) kg/day) from 28 kg to slaughter and food intake (1·62 v 1·48 v 1·48 (s.e. 0·028) kg/day) in the 28 to 55 kg weight range. Expander processing had no effect on ADG, food intake or food conversion ratio. The increase in byproduct level decreased (P < 0·01) killing-out proportion (723 v 705 v 710 (s.e. 3·94) g/kg) and carcass ADG (0·684 v 0·651 v. 0·635 (s.e. 0·012) kg/day) (P < 0·05). Expander processing decreased (P < 0·05) killing out proportion (707 v. 718 (s.e. 3·22) g/kg). In conclusion, increasing the by-product content of the diet decreased ADG while expander processing had no effect on growth performance.

This study was designed to investigate the size distribution of bovine steroidogenic luteal cells throughout pregnancy. Corpora lutea collected from three different stages of pregnancy were used. Luteal tissue was dissociated into single-cell suspension by enzyme treatments. Cells were stained for 3β-hydroxysteroid dehydrogenase (HSD) activity a marker for steroidogenic cells. The steroidogenic cells covered a wide spectrum of size ranging from 10 to 60 µm in diameter. There was a significant increase in mean cell diameter (P > 0·05) as pregnancy progressed. Mean diameter of 3β-HSD positive cells increased from 17·03 (s.e. 1·3) µm in the corpus luteum of early pregnancy to 33·38 (s.e. 2·4) µm in the corpus luteum of advanced pregnancy. The ratio of large (>22 µm in diameter) to small (10 to 22 µm in diameter) luteal cells was 0·32 : 1·0 in the early pregnancy, with the 10 to 22 µm cell size class predominant. However, the ratio of large to small luteal cells was increased to 6·49 : 1·0 µm as pregnancy advanced and 23 to 42 µm cell sizes become predominant. It is likely that small luteal cells develop into large cells as gestation progresses. Development of pregnancy is associated with an increase in size of steroidogenic luteal cells.

This paper reports the results of three experiments designed to attempt to improve the efficiency of milk production from diets based on a 1: 2 dry matter (DM) mixture of grass silage and whole-crop wheat (WCW) harvested at 550 to 600 g DM per kg and treated with 40 g urea per kg DM. In the first experiment a control diet of the forage mixture offered ad libitum with 9 kg fresh weight (FW) per day of a concentrate mix was compared with seven treatments in which the forage or the concentrates were varied. Eight multiparous cows were used in a four-period incomplete change-over design with 4-week periods. Caustic treatment of the WCW increased DM intake (P < 0·001) but tendencies for higher yields of milk and milk protein were not significant. Replacement of 2 kg FW concentrates per day by the same weight of molasses as a 24-h pre-soak of the forage (molasses pre-treatment) or added to the forage at feeding increased DM intake (P < 0·001) but did not increase milk yield or yield of milk solids. Molasses pre-treatment and molasses added to the concentrates reduced milk protein concentration (P < 0·05). Replacement of 2 kg FW concentrates per day by the same weight of ground wheat increased DM intake when added to the concentrates (P < 0·001) but not when added to the forage and milk yield was unaffected by either method of addition. The ground wheat tended to reult in higher milk protein concentrations than the molasses. Increasing the digestible undegradable protein content of the concentrates had no significant effect on food intake or milk production. In the second experiment diet digestibility and energy and nitrogen (N) balance were measured for the control diet and three of the treatments from experiment 1 in four multiparous cows in a 4 ✕ 4 Latin-square experiment with 5-week periods. There were no significant effects on food intake or milk yield. Caustic treatment reduced starch digestibility (P < 0·001) and increased neutral-detergent fibre (NDF) digestibility (P < 0·05) but had no effects on energy utilization. It also reduced N intake (P < 0·01) and urine N losses (P < 0·001) by reducing the ammonia concentration of the WCW. Molasses added to the forage or concentrates reduced milk protein concentration (P < 0·05) and digestibility of both starch and NDF (P < 0·05) but energy utilization was unaffected. In a further Latin-square experiment with the same treatments and four fistulated lactating cows, caustic treatment reduced daily mean ammonia concentration (P < 0·01) and increased daily mean pH (P < 0·01) in the rumen. It is concluded that these treatments did not generally improve the value of WCW although caustic treatment increased milk yield and milk protein yield consistently but not significantly. However practical problems may limit its use on the farm. Urea-treated WCW must be accepted as being a relatively low-energy food although with high intake characteristics, and future work should concentrate on evaluating WCW harvested at an earlier stage of maturity.