Environmental issues, linked to the production of animals in lowland farming systems, are reviewed, using pigs, poultry and dairy cattle as examples. The principal livestock production factors influencing their environmental impact are identified as the balance between different farm animal types and the husbandry practices used for these species, the variable potential which exists for the recycling of wastes and the modification of inputs to systems, the extent to which animal production can be integrated into more holistic farming systems and the impact of livestock on 'wildlife' (plant and animal) biodiversity. The production of large quantities of nitrogenous waste, resulting from the importation of large quantities of nitrogen to intensive animal production units, is identified as the major environmental problem for lowland animal production. The development of-systems which allow these waste products to be re-used at sites of primary crop production is seen as a sustainable solution to this problem.

To investigate the effects of energy source and protein level of diets on milk protein content, 12 multiparous Holstein-Friesian cows were used in a 4 × 4 Latin square change-over experiment with 4-week periods. Four diets were offered, with ad libitum silage as proportionately 0·40 of the diet, and the remaining 0·60 as one of four concentrates, two based on barley and two on molassed sugar-beet pulp. Two protein levels were achieved by altering the amounts of digestible undegraded protein in the concentrates, with all diets formulated to supply equal quantities of rumen degradable protein. There was no effect of diet on dry-matter intakes. Both starch and high dietary protein levels significantly increased milk protein concentration (P < 0·05), but had no effects on milk fat and lactose concentrations. Mean milk yields were significantly higher (P < 0·05) with increased dietary protein. Dietary protein significantly affected the yields of milk protein (P < 0·01) and lactose (P < 0·05) but not that of fat. Urinary allantoin excretion was significantly greater with both high protein (P < 0·05) and starch-based diets (P < 0·05). No significant interaction effects were found. It is concluded that dietary effects were due largely to differences in supply of rumen degradable protein; increases in milk protein concentration were therefore brought about by increasing the protein supply to the animal.

The positive effect of administration of growth hormone (GH) on milk production and its presence in selected dairy cattle lines of higher GH concentrations prompted an examination of the presence of restriction fragment length polymorphism (RFLP) in the growth hormone gene using the enzyme Taq/ and to investigate associations between this polymorphism and milk production traits. Blood was sampled from 251 Italian Holstein-Friesian cows. Three fragment bands, arbitrarily denoted A, B and E, of 6·2, 5·2 and 1·9 kilobase (kb), respectively, were observed. Their combinations exhibited six patterns, AA, AB, ABE, AE, BB and BE with frequencies of 64·5, 24·3, 2·4, 6·8, 1·4 and 0·4%, respectively. The statistical analysis was performed using linear mixed animal models. The results indicated an effect of the GHgene polymorphic Taq/restriction fragment on 305-day productions of milk, fat and protein; the low frequency pattern AE showed productions inferior to those for AA or AB patterns. Effect estimates of AA, AB and AE were, respectively, 200 (s.e. 215), 218 (s.e. 267) and -910 (s.e. 380) kg for milk production, 7·75 (s.e. 7·98), 16·10 (s.e. 9·79) and -22·14 (s.e. 14·42) kg for fat production, and 6·78 (s.e. 6·21), 8·57 (s.e. 7·58) and -20·74 (s.e. 11·14) kg for protein production. The average substitution effect estimates of E were -891 (s.e. 278), -26·56 (s.e. 10·16) and -24·50 (s.e. 9·43) kg for milk, fat and protein yields, respectively. In conclusion, these results suggest that the E fragment deserves further designed and specific study.

A study was conducted to examine the effect of fine grinding or sodium hydroxide treatment of wheat, and increasing concentrate food level, on milk production. Two concentrates based either on ground wheat (450 g/kg, GW) or sodium hydroxide treated wheat (500 g/kg, SW) were offered at four concentrate levels of 2·5, 5·0, 7·5 and 10·0 kg dry matter (DM) per day to 24 dairy cows in a three-period, change-over design experiment. On average, across all concentrate food levels, silage DM intake was significantly (P < 0·01) higher with the SW concentrates, reflecting a significantly lower silage substitution rate with SW relative to GW concentrates (P < 0·01). Milk yield was also significantly higher with the SW concentrates (P < 0·05), although marginal responses to increased concentrate food level were similar (P > 0·05). Milk protein concentration increased linearly with increasing concentrate food level (P < 0·001), with a significantly greater response with the GW relative to the SW concentrate (0·59 v. 0·24 g/kg (P < 0·05) increase per kg additional concentrate). However, milk protein concentration was also significantly lower with the GW concentrate at low food levels (P < 0·05). Milk fat concentrations were similar with the two concentrate types with significant reductions in milk fat concentration with increasing concentrate food level (P < 0·05). Blood urea (P < 0·001) and β-hydroxybutyrate (P < 0·05) concentrations were significantly lower in animals offered the SW concentrate. Apparent digestibility coefficients were unaffected by either concentrate type or concentrate food level (P > 0·05), although modified acid-detergent fibre apparent digestibility was significantly reduced with increasing concentrate food level (P < 0·001). Results indicate that, at similar levels of concentrate feeding, silage intake and milk yield were significantly greater with SW compared with GW concentrates (P < 0·05 or greater). Increases in milk protein concentration with increasing concentrate food level were significantly greater with GW than with SW concentrates (P < 0·05).

The extent to which grazing intensities of animal production systems in the uplands of the United Kingdom cause impacts on vegetation, soils, birds, mammals and invertebrates, and influence landscape value and water quality are reviewed. It is argued that these impacts need to be considered in an integrated manner in relation to their responses at the field and landscape scales. Evidence is presented which suggests that a range of grazing intensities is required to obtain significant benefits to the natural heritage. This suggests that new approaches are required to the mechanisms of delivering environmental benefits from grazing systems.

Acetone concentration in milk is correlated with subclinical and clinical ketosis and also with milk yield and reproductive efficiency. The purpose of this study was to assess a threshold of milk acetone at which cows tend to be adversely affected. The critical values were estimated on data from 11690 lactations where the change in daily milk yield from weeks 1 to 2 to weeks 5 to 6 of lactation was evaluated in relation to different milk acetone concentrations. Daily milk yield tended to be reduced in cows with acetone concentrations between 0·7 and 1·4 mmol/l, but responses were not entirely consistent. At concentrations >l·4 mmol/l, daily milk yield was clearly reduced. Long-term milk yield (day 0 to 100 and day 0 to 200) was about 190 kg of 40 g/kg fat-corrected milk (FCM) less in cows with acetone concentrations >l·4 vis-à-vis <0·7 mmol/l. Reproductive efficiency was also impaired in cows with acetone concentrations >l·4 mmol/l; 4·9 days longer interval from calving to first service (first parity and parity 2+ cows) and 5·7 times a greater risk of cystic ovaries (parity 1), compared with cows with milk acetone <0·7 mmol/l. However, no significant effects on long-term milk yield or on reproductive efficiency were found in cows with acetone concentrations 0·7 to 1·4 vis-à-vis <0·7 mmol/l. The results suggest that 1·4 mmol/l acetone in milk may be used as the most important critical value, as higher concentrations are detrimental to productivity. The interval 0·7 to 1·4 mmol acetone per I milk may be used as a warning class, since early lactation yield may be reduced.

An experiment was conducted to examine the effect of method and level of concentrate feeding on milk production characteristics in dairy cows offered a grass silage-based diet. Twelve treatments were used with 24 Holstein/Friesian dairy cows in a four-period change-over design experiment. Treatments consisted of three methods of concentrate feeding (twice daily, four times daily or complete diet) and four levels of concentrate feeding (2, 4, 6 or 8 kg/day). Offering a complete diet significantly reduced silage substitution rate (0·28 kg silage dry matter (DM) per kg concentrate DM) compared with concentrates offered twice daily (0·50 kg silage DM per kg concentrate DM; P < 0·01). Method of concentrate feeding had no significant effect on milk yield or milk fat concentration (P > 0·05), although the response in milk protein concentration to increased concentrate food level was significantly greater with twice and four times daily concentrate feeding than with complete diet feeding (0·59, 0·56 and 0·44 g/kg per kg increase in concentrate food level; P < 0·05). Increasing concentrate inclusion level significantly reduced silage DM intake (P < 0·001) but significantly increased (P < 0·001) total DM intake. Milk yield and milk protein concentration were significantly increased (P < 0·001) and milk fat concentration significantly reduced (P < 0·02) with increasing concentrate inclusion level. Total ration apparent digestibility coefficients were unaffected (P > 0·05) by either method of feeding or concentrate inclusion level. However, modified acid-detergent fibre apparent digestibility decreased significantly with increasing concentrate inclusion (P < 0·01). Results suggested that offering complete diets, with concentrates comprising on average 0·33 of total DM intake, increased both silage and total DM intake although this was not reflected in either increased milk yield or improvements in milk composition.

Countries, which import a significant amount of semen, embryos and animals, are faced with the problem of how properly to evaluate the animals in the national evaluation system when information on the foreign parents is generally missing. Additional problems arise when the foreign parents obtain an evaluation, usually on the basis of progeny, in the country of import with the result that there are two published evaluations for the animals in question. This paper presents a post-iterative method of incorporating foreign information into home country evaluations. The foreign information is initially converted to the same scale and base as in the home country using procedures recommended by the International Bull Evaluation Service. The method consists essentially of calculating a combined evaluation for animals with home and foreign information as a weighted average of yield deviations, parent averages and progeny contributions from the foreign and home countries. The combined evaluations are used to adjust the evaluations of progeny on the basis of formulae derived from the usual mixed model equations. The same principles were used to combine reliabilities from the respective home and foreign reliabilities. The results from the application of the method to the United Kingdom (UK) Holstein Friesian population are presented. There was re-ranking of both bulls and cows, especially foreign bulls with few UK daughters.

Five published equations were compared on their ability to adjust different patterns of lactation curves. The equation y(t) = a exp(b1t'2/2 + b2/t – c(l + t'/2)t') with t' = (t – 21·4)/l00 (i) was retained because of the quality of the adjustment and the absence of convergence problems when applied on individual curves. Including an effect of season (SE) and an effect of pregnancy (PE) improved the quality of individual adjustments (no. = 339). The final equation was equal to (i) + SE + PE with SE = a + b cos (2π(wc + w - l)/52) + c sin(2n(wc + w - 1)/52), with wc= week of the year at calving, w = week after calving and a = 0·0065, b = -1·26 and c = 0·374, and PE = a (wp –18) e-bwp, with w p = week of pregnancy and a, b fixed parameters. Individual cow characteristics were used to analyse equation (i) parameters. The predictions obtained with this equation and several predictive functions of the equation (i) parameters, which differed in the way they included or not the potential of production, were compared. With no indication of this potential, the prediction was very poor. With the initial production (mean production of the 4th, 5th and 6th days of lactation) as an estimate of this potential, 75% of the lactations had the median of absolute values of errors less than 2·95 kg/day. The results were highly improved by using the yield during the 5th week of lactation. The threshold of 2·95 was reduced to about 2 kg/day. The quality of the individual prediction was better for primiparous than for multiparous cows, and for French Friesian and Montbeliarde cows than for pure or crossbred Holstein cows. Although individual predictions were not always satisfactory, they provided excellent agreement when averaged per group (20 cows).

Two experiments were carried out to evaluate the effect of incremental levels of Gliricidia sepium (gliricidia) and Leucaena leucocephala (leucaena) on forage intake, diet apparent digestibility, microbial nitrogen supply and live-weight changes in cattle. In each experiment, 20 intact and five fistulated crossbred steers (Bos taurus × Bos indicus) were used. Napier grass basal diet was offered ad libitum alone or supplemented with 7·5, 15, 22·5, or 30 g dry matter (DM) per kg metabolic body weight (M075) gliricidia (experiment 1) or leucaena (experiment 2). In experiment 1, total DM intake increased but not significantly with gliricidia supplementation (5·2, 5·1, 5·2, 5·4, 5·7 (s.e.d. 0·21) kg/day) while intake of napier grass was depressed linearly (5·2, 4·7, 4·5, 4·3, 4·2 (s.e.d. 0·21) kg/day; P < 0·05). In experiment 2, supplementation with leucaena increased the total DM intake linearly without depressing the intake of napier grass (5·2, 5·8, 6·2, 6·6, 6·7 (s.e.d. 0·31) kg/day; P < 0001 and 5·2, 5·3, 5·3, 5·3, 5·0 (s.e.d. 0·21) kg/day respectively). Neither gliricidia nor leucaena supplementation affected the apparent digestibility of the diet or in sacco DM degradation characteristics of the forages. Rumen ammonia and live-weight gain were increased linearly (P < 0·05) by supplementation from 130 to 215mg/l (experiment 1) and 75 to 113mg/l (experiment 2), from 306 to 478 g/day (experiment 1) and from 538 to 850 g/day (experiment 2), respectively. However, since the responses were linear, further experiments are required to quantify the responses to higher levels of these legume supplements.

Effects of feeding a protein supplement to dairy cows during the dry period on performance during the following lactation were investigated in two experiments. Holstein-Friesian cows were paired towards the end of lactation, and, after drying off, one of each pair received a typical dry cow management regime of ad libitum grass silage (experiment 1), or a mix of grass silage and distillers' grains or pressed beet pulp (experiment 2). The other cows were offered restricted access to the same basal diet, together with ad libitum access to barley straw and 0·5 kg/day high protein maize gluten meal. During the following lactation, animals from both groups were treated without reference to dry period treatment, and were offered equal access to the same lactation diet. Data were analysed by analysis of variance of experiment means and by parallel curve analysis using sample means. In experiment 1, milk yields were similar (27·2 v. 27·9 (s.e.d. 2·12) kg/day for control and supplemented animals respectively) but milk protein yields, and hence concentrations, were significantly higher (P < 0·001) from supplemented animals (28·9 v. 31·8 (s.e.d. 0·58) g/kg). In experiment 2, milk yields were significantly higher (P < 0·001) from supplemented animals (mean 33·3 v. 35·4 (s.e.d. 1·66) kg/day; however, milk protein yields were also significantly increased (P < 0·001) and the change in milk protein concentration was small. No difference in dry-matter intake was recorded in a subset of animals during early lactation in experiment 2. It is hypothesized that the maternal labile body protein pool was maintained or replenished during the dry period by the provision of the protein supplement, and that this had a significant effect on subsequent lactation performance.

An 18-week experiment investigated the effects of type of diet and frequency of watering on the performance of growing cattle given food at maintenance (metabolizable energy (ME) allowance (MJ) = 8·3 + 0·091 M where M = live weight of the animal). Three diets with 20: 80 (low, L), 50:50 (medium, M) and 80: 20 (high, H) roughage to concentrate ratios (10·2, 7·8 and 5·4 MJME per kg dry matter) were used in combination with free access to water for 2·5 h once daily or once every 3rd day. A completely randomized-block design with a 3 (diets) × 2 (watering frequencies) factorial arrangement of treatments was used. Five animals were allocated per treatment (average weight 245 (s.d. 21·6) kg).

Dry-matter intake increased as the roughage content of the diet increased (P < 0·05) but was not affected by the watering regime (P > 0·05). Total water intake was found to be positively correlated with dry-matter intake and increased with dietary roughage level. Animals watered daily drank more (P < 0·05) water (12·6 v. 10·0 kg/day) than those watered every 3rd day. The apparent digestibility of the diets decreased as the amount of roughage increased (diet L 734g/kg, M471 g/kg and H 433 g/kg). Similar apparent digestibilities were observed under the two watering frequencies (546 g/kg and 547 g/kg).

Type of diet significantly (P < 0·05) affected the final weights of the steers. Animals given diet H were proportionately 0·07 heavier than those offered diet L corresponding to total weight changes of −0·8 and +18·4 kg respectively. Carcass weight, backfat thickness and eye muscle area were not affected by the treatments. The chemical composition of the 9 to 11th rib joint was only affected by diet H which gave less fat (P < 0·05). Water, fat, protein and ash content averaged 607,118,190 and 84 g/kg respectively.

The main purpose of this work was to compare two breeds of improved rams (Suffolk and Merino Precoce) with the local Galego Bragangano breed for the production of crossbred slaughter lambs and to evaluate which cross was more adapted for meat production from the local breed in locations in the north-east of Portugal. The experiment was carried out over a 4-year period in three locations: (1) a farm with an intensive management; (2) an upland farm (400 to 600 m); and (3) a hill farm (above 800 m). Within each flock with 90 Galego Bragangano ewes, two rams of each of the sire breeds were used: Galego Bragangano, Suffolk and Merino Precoce. The lambs were slaughtered at 20 and 40kg, to obtain the carcass weight range of 8 to 14kg. The left sides of 151 carcasses were dissected into muscle, subcutaneous fat, intermuscular fat and bone. The lambs from location 1 had the highest carcass muscle proportion and the lowest carcass intermuscular fat proportion; their differences over locations 2 and 3 were 13 and 16 g/kgfor muscle proportion and 11 and 19 g/kgfor intermuscular fat proportion, respectively. The differences between breeds were relatively small and not significant. However the Suffolk crosses had less kidney, knob and channel fat than the other genotypes (5 and Uglkg less than Merino crosses and Bragangano, respectively). The Suffolk crosses tended to have less body fat.

A study was carried out to evaluate the effect of horizontal walking upon CO2 production rate by the carbon dioxide dilution rate technique. This was used as an indicator of animal energy expenditure. Two groups of three 18-month-old Aberdeen-Angus steers were assigned to two experiments. Average weights were 290 (s.e. 7·6) kg and 285 (s.e. 1·0) kg for experiments 1 and 2 respectively. Animals were allocated to individual pens and given 5·0 and 4·5 kg dry matter of a mixed diet for experiments 1 and 2 respectively. After a 45-day training period they were assigned to three walking treatments: 0 (T0), 3 (T3) and 6 (T2) km at 3 km/hfor 3 days in a Latin square design (3 × 3). 14C labelled sodium bicarbonate (5·4 μCi/h), diluted in carbonate-bicarbonate buffer sterile solution 0·1 mol/l, was infused for 92 h intraperitoneally with portable peristaltic pumps carried by the animals. The CO2 production rate was calculated as the ratio between the rate of infusion (μCi/h) and the specific activity of CO2 (μCi/ml CO2) in saliva samples, which were taken, in experiment 1, as an average of the day (09.00 to 16.00 h) and the night (16.00 to 09.00 h of the following day). In experiment 2 the day was divided as follows: prior to activity (09.00 to 13.00 h), activity (14.00 and 15.00 h) and post activity (16.00 h). CO2 production rate (ml CO2 per h per kg M0·75) at resting was 817 (412 kj/kg M0·75), increasing during walking to 1·46 of the resting level (T1 and T2, experiment 2) with no differences between the 1st and 2nd h of activity. One hour post activity, the CO2 production rate returned in T2 to the level of T0 but in T2 remained at 1·28 times that of T0. The average CO2 production rate during a complete day or night (experiment 1) was not affected significantly by the activity. Assuming that CO2 production rate during walking is 1·46 of resting (experiment 2) and remains at that level even at lower speeds, it can be estimated that a daily 6 km walk would increase resting energy expenditure from 1·04 when walking takes 2 h, as in this experiment (3 km/h), to 1·11 when the animal spends 6h(1 km/h).

In a lifetime study of spring born cattle managed in a 20-month beef system, four feeding treatments were imposed during the 5-month winter period and two grass heights (low 6 to 8 cm, high 8 to 10 cm) during the subsequent grazing period. Three slaughter weights were imposed over the grazing period, early, mid and late, at an average of 67,110 and 154 days post turn-out. A multi-factorial design was used with three animal factors — maturity (early maturing Hereford crosses v. late maturing Charolais crosses), sex (heifer v. steer) and method of rearing (suckled calves v. bucket-reared calves). There were significant differences in growth rate for both sex and maturity (P < 0·001) and a highly significant negative effect of winter food level on summer growth rate (P < 0·001), the growth rate of food treatment 4 being proportionately 0·61 of that treatment 1.

Sward height significantly influenced summer growth rate (P < 0·001) but showed no interaction with winter food level in any of the three grazing periods. Growth rates increased over the summer but differences between winter food treatments decreased with daily gains for food treatment 4 being proportionately 0·44, 0·81 and 0·84 of food treatment 1 as the grazing season progressed.

Eliminating winter feeding treatment as a factor and including condition score at turn-out as a co-variate improved the variation explained and reduced differences in growth rate for the main effects with only the main effect of grass height remaining significant. This suggests that the condition of animals at turn-out in conjunction with the subsequent grazing sward height provides a simple practical guide to subsequent animal performance.

Coopworth sheep from the high and low backfat selection lines and from one random-bred line developed at Invermay, New Zealand, were used to examine the interaction between the degree offatness of sheep and a period of maintenance feeding (from 20 to 35 weeks of age), in terms of total fat, carcass lean tissue, bone and viscera weights, relative to the empty body weight measured in computer-aided tomography scans. A similar study was done for weights of subcutaneous, intermuscular and internal (abdominal) fat partitions relative to total fat weight. During two a d libitum feeding periods (front 10 to 20 and from 35 to 43 weeks of age) changes in the same components relative to the ‘whole’ (empty body weight or total fat, depending on the case) were expressed in allometric terms.

Only the allometric growth coefficient for total fat weight was significantly affected by the period of restricted feeding, decreasing after this period. No line or sex effect was observed on any growth coefficient. As a result, there were no changes in differences between treatments over the ad libitum feeding periods, with fat animals and females having significantly higher proportions of fat, while females also had a significantly lower proportion of intermuscular fat.

During maintenance feeding, line and sex differences in dissectible component weights did not change significantly, except in terms of intermuscular fat, with the males showing an increasingly larger proportion than the females.

In each of three experiments, 60 Limousin × Holstein Friesian bulls, initially of 191, (s..e. 5·0), 177 (s.e. 5·5) and 210 (s.e. 7·2) kg mean live weight for experiments 1, 2 and 3 respectively, were offered maize silage ad libitum plus one of three barley-based, isonitrogenous compound diets containing 200 g rapeseed meal, 575 g maize-gluten feed or 100 g fish meal per kg fresh weight. Compound diets were given at the rate of 3·0 kg (fresh weight) per head per day up to 320 kg live weight and at 3·3 kg per head per day from 320 kg to 420 kg live weight. In experiment 3, when bulls were taken through to slaughter, the compound diet was further increased to 3·5 kg per head per day from 420 kg live weight to slaughter. Experiments 1 and 2 were conducted over 128 and 194 days, with mean live weights at the end of the experiments of 347 (s.e. 17·2) and 414 (s.e. 24·2) kg respectively. In experiment 3, bulls were slaughtered at 16 months of age at a mean live weight of 544 (s.e. 25·0) kg.

In experiment 1, the fish-meal diet gave the highest rate of daily live-weight gain at 1·34 compared with 1·17 and 1·16 (s.e.d. 0·041) kg for rapeseed-meal and maize-gluten diets, respectively. In experiments 2 and 3, daily gains were similar on all treatments at 1·21, 1·19 and 1·26 (s.e.d. 0·036) kg and 1·06, 1·09 and 1·11 (s.e.d. 0·035) kg for the rapeseed-meal, maize-gluten and fish-meal diets respectively. Total daily dry-matter intakes did not show consistent trends: in experiment 1, intakes were 5·8, 5·6 and 5·8 kg/day, in experiment 2, 6·2, 6·2 and 6·3 kg/day; and in experiment 3, 7·1, 7·3 and 7·1 kg/day for rapeseed-meal, maize-gluten and fish meal, respectively. The higher intakes of dry matter in experiment 3 were due to the greater intakes of maize silage by bulls taken to heavier weights in this experiment.

The energy expenditure of freely grazing cattle was investigated in the National Institute of Agricultural Technology and Agricultural Science College of Balcarce, Argentina (37° 45'S, 58° 18'W), by the CO2 entry rate technique. Two experiments were carried out in the autumn in March 1994 (experiment 1) and in April 1995 (experiment 2) with animals prepared with catheters in the parotid gland (collection of saliva) and into the peritonea for infusion of a solution of 14C. Six Angus steers (259 (s.e. 11) kg) were used in experiment 1 and seven (298 (s.e. 36) kg) in experiment 2. In experiment 1 animals grazed ryegrass pastures for 5·5 h in two periods of 1·5 h in the morning and of 4 h in the afternoon and in experiment 2 animals grazed oat pasture in one period of 1 h in the morning. Twenty hours before and during the experiments a solution ofNaH14CO3 was infused at a rate of 9·4 (experiment 1) and 8·1 (experiment 2) μiCi/h for 48 h with portable peristaltic pumps carried by each animal. Saliva samples were collected at least after the first 20 h of infusion. The first sample was collected in the corral just before grazing and two samples were collected in each grazing period. Also, in experiment 1 three samples were taken during resting (noon, 1 h after grazing and the next morning). In addition, bite frequency, pasture availability, plant height, in vitro digestibility and crude protein were measured. Carbon dioxide production was calculated as the ratio between the rate of infusion of 14C (μCi/h) and the specific activity of CO2 (μCi/l CO2) in saliva samples. Bite frequency was 59 and 28 bites per min on the respective pasture of ryegrass (148 g dry matter (DM) per m2 and 10·5 cm height) and oat (228 g DM per m2 and 27 cm height). Energy expenditure (EE, kJ/h per kg M0·75) in corrals was 14·9 (experiment 1) and 14·3 (experiment 2), increasing to 22·6 (proportionately 0·52) when grazing at 59 bites per min and to 16·6 (0·16) when grazing was at 28 bites per min. One hour after grazing at 59 bites per min (experiment 1) the EE was as high as during grazing, and in the next morning (after 5·5 h) of grazing remained at 19·7 kJ/h per kg M0·75 (0·32). No differences in energy expenditure were found between periods of grazing in experiment 1. It was concluded that the increase in energy expenditure of cattle due to the activity of grazing depends on the rate of biting. Grazing for 10 h at a moderate rate may boost EE proportionately by only 0·06, however grazing at the highest rates could easily add proportionately 0·20.

An investigation was conducted into the effect of buserelin treatment on day 12 post mating on ovarian function and plasma progesterone and oestradiol-17 ji concentration in ewes. Changes in ovarian structures were determined, at laparoscopy or after slaughter, 48 h after treatment in 38 control and 41 ewes given an injection of 4 jig buserelin on day 12 post mating (day 0 = day of oestrus). Ovulation rate was higher in buserelin treated (2·76) than in control (1·87) ewes (P < 0·05). Moreover, 41 of the 113 corpora lutea observed in the buserelin group were the result of ovulations that had occurred within 48 h post treatment, compared with two of 71 in the controls (P < 0·05). Blood samples were collected from 10 ewes in each treatment group for hormone analysis. Mean progesterone concentrations tended to be higher, in samples collected from 2 to 6h post treatment, in the buserelin group (4·31 (s.e. 1·32) p.g/1) compared with the control group (3·71 (s.e. 1·44) fig/l. Furthermore, overall mean oestradiol concentrations were lower, during days 13, 14 and 15 post mating, in buserelin treated (0·72 (s.e. 0·26) ngfl) than in control (0·94 (s.e. 0·50) ng/l) ewes (P < 0·05). The results of this study indicate that buserelin treatment on day 12 post mating changes ovarian function.

Jugular and ovarian venous profiles of progesterone and endometrial progesterone concentrations were measured in pregnant and non-pregnant ewes. Mean jugular concentrations were not significantly different (3·12v.2·39 μg/l). The incidence of progesterone concentrations above a chosen threshold was significantly greater in the ovarian vein than in the jugular vein (P < 0·05) and was greater (P > 0·05) in the ovarian venous plasma of pregnant than of non-pregnant ewes. Mean endometrial progesterone concentrations were significantly higher in pregnant than in non-pregnant ewes (T < 0·05). It is suggested that the lower progesterone concentrations in the ovarian veins of non-pregnant ewes may have a rôle in pregnancy failure and that ovarian venous concentrations may influence endometrial progesterone concentrations.