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The effect of GnRH analogue (buserelin) treatment on day 12 post mating on ovarian structure and plasma progesterone and oestradiol concentration in ewes

Published online by Cambridge University Press:  02 September 2010

N. F. G. Beck ,
M. Jones ,
B. Davies ,
G. E. Mann  and
A. R. Peters
N. F. G. Beck
Affiliation:
Department of Agricultural Sciences, University of Wales, Aberystwyth SY23 3DD
M. Jones
Affiliation:
Department of Agricultural Sciences, University of Wales, Aberystwyth SY23 3DD
B. Davies
Affiliation:
Department of Agricultural Sciences, University of Wales, Aberystwyth SY23 3DD
G. E. Mann
Affiliation:
Department of Physiology and Environmental Science, University of Nottingham, Sutton Bonington LE12 5RD
A. R. Peters
Affiliation:
Department of Animal Health, Royal Veterinary College, Potters Bar EN6 1NB
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Abstract

An investigation was conducted into the effect of buserelin treatment on day 12 post mating on ovarian function and plasma progesterone and oestradiol-17 ji concentration in ewes. Changes in ovarian structures were determined, at laparoscopy or after slaughter, 48 h after treatment in 38 control and 41 ewes given an injection of 4 jig buserelin on day 12 post mating (day 0 = day of oestrus). Ovulation rate was higher in buserelin treated (2·76) than in control (1·87) ewes (P < 0·05). Moreover, 41 of the 113 corpora lutea observed in the buserelin group were the result of ovulations that had occurred within 48 h post treatment, compared with two of 71 in the controls (P < 0·05). Blood samples were collected from 10 ewes in each treatment group for hormone analysis. Mean progesterone concentrations tended to be higher, in samples collected from 2 to 6h post treatment, in the buserelin group (4·31 (s.e. 1·32) p.g/1) compared with the control group (3·71 (s.e. 1·44) fig/l. Furthermore, overall mean oestradiol concentrations were lower, during days 13, 14 and 15 post mating, in buserelin treated (0·72 (s.e. 0·26) ngfl) than in control (0·94 (s.e. 0·50) ng/l) ewes (P < 0·05). The results of this study indicate that buserelin treatment on day 12 post mating changes ovarian function.

Keywords

buserelinewesoestradiolovariesprogesterone
Type
Research Article
Information
Animal Science , Volume 63 , Issue 3 , December 1996 , pp. 407 - 412
Copyright
Copyright © British Society of Animal Science 1996

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References

Abraham, G. E., Swerdloff, R., Tulchinsky, D. and Odell, W. D. 1971. Radioimmunoassay of progesterone. Journal of Clinical Endocrinology and Metabolism. 32: 619–624.CrossRefGoogle ScholarPubMed
Beck, N. F. G., Peters, A. R. and Williams, S. P. 1994. The effect of GnRH agonist (buserelin) treatment on day 12 post mating on the reproductive performance of ewes. Animal Production. 58: 243–247.CrossRefGoogle Scholar
Bolet, G. 1986. Timing and extent of embryonic mortality in pigs, sheep and goats: genetic variability. In Embryonic mortality in farm animals (ed. Sreenan, J. M., Diskin, M. G.), pp. 13–43. Martinus Nijhoff, The Hague.Google Scholar
Drew, S. B. and Peters, A. R. 1994. Effect of buserelin on pregnancy rates. Veterinary Record. 134: 267–269.CrossRefGoogle ScholarPubMed
Foster, J. P., Lamming, G. E. and Peters, A. R. 1980. Short term relationships between LH, FSH and progesterone concentrations in post-partum dairy cows and the effect of GnRH injection. Journal of Reproduction and Fertility. 59: 321–327.CrossRefGoogle Scholar
Jubb, J. F., Abhayaratne, D., Malmo, J. and Anderson, G. A. 1990. Failure of an intramuscular injection of an analogue of gonadotrophin releasing hormone 11 to 13 days after insemination to increase pregnancy rates in dairy cattle. Australian Veterinary journal. 67: 359–361.CrossRefGoogle ScholarPubMed
Lukaszewska, J. and Hansel, W. 1980. Corpus luteum maintenance during early pregnancy in the cow. Journal of Reproduction and Fertility. 59: 485–493.CrossRefGoogle ScholarPubMed
Macmillan, K. L., Day, A. M., Taufa, V. K., Gibb, M. and Pearce, M. G. 1985. Effects of an agonist of gonadotrophin releasing hormone (buserelin) in cattle. I. Hormone concentrations and oestrous cycle length. Animal Reproduction Science. 8: 203–212.CrossRefGoogle Scholar
Macmillan, K. L., Taufa, V. K. and Day, A. M. 1986. Effects of an agonist of gonadotrophin releasing hormone (buserelin) in cattle. III. Pregnancy rates after a post-insemination injection during metoestrus and dioestrus. Animal Reproduction Science 11: 1–9.CrossRefGoogle Scholar
Macmillan, K. L. and Thatcher, W. W. 1988. Effects of GnRH analogue on ovarian follicular dynamics and corpus luteum lifespan in cattle. Journal ofAnimal Science 66: (suppl. 1) 432 (abstr.).Google Scholar
McMillan, W. H., Knight, T. W. and Macmillan, K. L. 1986. Effects of gonadotrophin releasing hormone (buserelin) on sheep fertility. Proceedings of the New Zealand Society for Animal Production. 46: 161–163.Google Scholar
Mann, G. E. and Lamming, G. E. 1995a. Effects of treatment with buserelin on plasma concentrations of oestradiol and progesterone and cycle length in the cow. British Veterinary Journal 151: 427–432.CrossRefGoogle ScholarPubMed
Mann, G. E. and Lamming, G. E. 1995b. Effect of the level of oestradiol on oxytocin induced prostaglandin F2a release in the cow. Journal of Endocrinology. 145: 175–180.CrossRefGoogle ScholarPubMed
Mann, G. E., Lamming, G. E. and Fray, M. D. 1995. Plasma oestradiol and progesterone during early pregnancy in the cow and the effect of treatment with buserelin. Animal Reproduction Science. 37: 121–131.CrossRefGoogle Scholar
Mann, G. E. and Picton, H. M. 1995. Ovarian and uterine effects of a single buserelin injection on day 12 of the oestrous cycle in the cow. Journal of Reproduction and Fertility, Abstract Series 15: 61 (abstr.).Google Scholar
Peters, A. R., Drew, S. B., Mann, G. E., Lamming, G. E. and Beck, N. F. G. 1992.Experimental and practical approaches to the establishment and maintenance of pregnancy. Journal of Physiology and Pharmacology 43: (suppl. 1) 143–152.Google Scholar
Rettmer, I., Stevenson, J. S. and Corah, L. R. 1992. Endocrine responses and ovarian changes in inseminated dairy heifers after an injection of GnRH agonist 11 to 13 days after estrus. Journal ofAnimal Science. 70: 508–517.Google ScholarPubMed
Ryan, D. P., Snijders, S., Condon, T., Grealy, M., Sreenan, J. and O'Farrell, K. J. 1994. Endocrine and ovarian responses and pregnancy rates in dairy cows following the administration of a gonadotrophin releasing hormone analog at the time of artificial insemination or at mid-cycle post insemination. Animal Reproduction Science. 34: 179–191.CrossRefGoogle Scholar
Sheldon, I. M. and Dobson, H. 1993. Effects of gonadotrophin releasing hormone administered 11 days after insemination on the pregnancy rates of cattle to the.first and later services. Veterinary Record. 133: 160–163.CrossRefGoogle ScholarPubMed
Thatcher, W. W., Macmillan, K. L., Mansen, P. J. and Drost, M. 1989. Concepts for regulation of corpus lutuem by the conceptus and ovarian follicles to improve fertility. Theriogenology 31: 149–164.CrossRefGoogle Scholar
Wilmut, I., Sales, D. I. and Ashworth, C. J. 1986. Maternal and embryonic factors associated with prenatal losses in mammals. Journal ofReproduction and Fertility. 76: 851–864.CrossRefGoogle Scholar