In its early years the Independent church at Rothwell had groups of members in the Kimbolton area on the Huntingdonshire and Bedfordshire border: also in and around Bedford. Those in the Kimbolton area became members of the Kimbolton Independent church when it was formed in 1692 by a peaceable separation from the Rothwell church. Those in the Bedford area became part of the Bedford Independent church (later the Southill Independent church) when this was formed in 1693 by a peaceable separation from the Rothwell church.

The Rothwell Church Book is very detailed and shows the following Bedfordshire persons joining the Rothwell church between March 1691 and December 1692: Mary Mee of Keysoe, Mary Barringum of Swineshead, Judin Virley his wife of Berry Fields (Keysoe), Sarah Eaton of Pertenhall, Susan Coales of Keysoe, Elizabeth Dawson of Riseley, Edward Marlin of Bletsoe, Mary Marlin of Bletsoe, Ann Gary of Bolnhurst, Rebecca Richards of Keysoe, Henry Wattford of Keysoe, Ann Wattford of Keysoe, John Arch of Bletsoe, William Medbury of Bletsoe, Elizabeth Bossett of Keysoe, Joseph Henson of Swineshead, Ann Hills of Bletsoe, William Woodham of Riseley, Samuel Gury of Bolnhurst, John Conquest of Colmworth, Thomas Wagstaff of Colmworth, Grace Cradoc of Keysoe, Elizabeth Conquest of Colmworth, William Carter of Kempston, Grace Pheasant of Berry Fields (Keysoe), John Johnson of Colmworth, Steven Rich of Keysoe, Samuel Booth of Bolnhurst, George Fisher of Colmworth, Samuel Shepherd of Pertenhall, Thomas Mores of Bletsoe, Thomas Wagstaff, senior, of Colmworth, Joseph Richards of Keysoe, Mary Milton of Colmworth, Farmer Casse of Colmworth, Mr Clark of Bedford, Mary Carter of Kempston, Thomas Fisher of Stagsden, Alice Sea of Keysoe, Sarah Woodham of Riseley, Alice Hopkins of Riseley, Luke Folick of Bolnhurst, Elizabeth Melton of Bolnhurst, Grace Cook of Bolnhurst, Joseph Barringer of Swinesliead, Thomas Woodham of Riseley, George Hull of Colmworth, Thomas Eaton of Pertenhall, Sarah Stiles of Bolnhurst, Susan Dauson of Riseley, Mrs. Richards of Keysoe, Elizabeth Henson of Swineshead, Susannah Tompson of Riseley, Edward Hains of Bolnhurst, Peter Shrewsbury of Kempston, Mrs. Charlton of Wootton Pillinge, John Henson of Swineshead, Sarah Clifton of Keysoe, Katharine Basingham of Keysoe.