Book contents
- Frontmatter
- Contents
- List of contributors
- Preface
- PART I PHYSIOLOGICAL AND MOLECULAR RESPONSES
- 1 A primer on insect cold-tolerance
- 2 Rapid cold-hardening: Ecological significance and underpinning mechanisms
- 3 Antifreeze and ice-nucleator proteins
- 4 Genomics, proteomics and metabolomics: Finding the other players in insect cold-tolerance
- 5 Cell structural modifications in insects at low temperatures
- 6 Oxygen: Stress and adaptation in cold-hardy insects
- 7 Interactions between cold, desiccation and environmental toxins
- PART II ECOLOGICAL AND EVOLUTIONARY RESPONSES
- PART III PRACTICAL APPLICATIONS
- Index
- References
4 - Genomics, proteomics and metabolomics: Finding the other players in insect cold-tolerance
from PART I - PHYSIOLOGICAL AND MOLECULAR RESPONSES
Published online by Cambridge University Press: 04 May 2010
Book contents
- Frontmatter
- Contents
- List of contributors
- Preface
- PART I PHYSIOLOGICAL AND MOLECULAR RESPONSES
- 1 A primer on insect cold-tolerance
- 2 Rapid cold-hardening: Ecological significance and underpinning mechanisms
- 3 Antifreeze and ice-nucleator proteins
- 4 Genomics, proteomics and metabolomics: Finding the other players in insect cold-tolerance
- 5 Cell structural modifications in insects at low temperatures
- 6 Oxygen: Stress and adaptation in cold-hardy insects
- 7 Interactions between cold, desiccation and environmental toxins
- PART II ECOLOGICAL AND EVOLUTIONARY RESPONSES
- PART III PRACTICAL APPLICATIONS
- Index
- References
Summary
Introduction
Our understanding of the physiological mechanisms of insect cold-hardiness relies on a rich history of discovery that has revealed the presence of cryoprotective agents, antifreeze proteins, ice-nucleating proteins, heat-shock proteins and modifications of the cell membrane. Such discoveries have usually targeted select metabolites, proteins, or fatty acids, and have successfully revealed the identity of many key agents that protect insects from injury at low temperature, yet we suspect that, in many cases, additional protective agents may be involved. In some cases this may mean the potential discovery of entirely new cryoprotective agents or discovery of roles for a multitude of chemical modifications that may accompany a change in a single compound that has already been well documented. This is indeed the power of the “-omics” approach, discovery of large-scale changes at levels of the transcript (genomics or transcriptomics), protein (proteomics), or metabolites (metabolomics). These approaches are especially good for seeing the big picture. What novel genes or gene pathways are turned on or off? What proteins are uniquely synthesized or post-translationally modified in respond to the cold? What is the full range of metabolic change that occurs in response to cold, and what metabolic pathways are affected? These are the sorts of questions that can be uniquely answered by genomics, proteomics and metabolomics. Clearly, all of these approaches mark, not the end of the discovery phase, but the beginning.
- Type
- Chapter
- Information
- Low Temperature Biology of Insects , pp. 91 - 115Publisher: Cambridge University PressPrint publication year: 2010
References
, , and (2006). Cross-species microarray hybridizations: a developing tool for studying species diversity. Trends in Genetics 23, 200–207.CrossRefGoogle Scholar
and (1995). Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society, Series B (Methodological) 57, 289–300.Google Scholar
, , , and (1988). Heat shock protection against cold stress of Drosophila melanogaster. Molecular and Cellular Biology 8, 3550–3552.CrossRefGoogle ScholarPubMed
and (1990). Activation of phosphorylase: response to cold and heat stress in the flesh fly, Sarcophaga crassipalpis. Journal of Insect Physiology 36, 549–554.CrossRefGoogle Scholar
and (1992). Reduction of cold injury in flies using an intermittent pulse of high temperature. Cryobiology 29, 138–143.CrossRefGoogle Scholar
and (1989). Intermediary energy metabolism during dormancy and anoxia in the land snail, Otala lactea. Physiological Zoology 62, 1015–1030.CrossRefGoogle Scholar
, , , , and (2007). Proteomic profiling of a parasitic wasp exposed to constant and fluctuating cold exposure. Insect Biochemistry and Molecular Biology 37, 1177–1188.CrossRefGoogle ScholarPubMed
, , , and (2004). A prominent role for the CBF cold response pathway in configuring the low temperature metabolome of Arabidopsis. Proceedings of the National Academy of Sciences, USA 101, 15243–15248.CrossRefGoogle ScholarPubMed
, , , and (1991). Cold shock and heat shock. In Insects at Low Temperature, ed. , and : New York: Chapman and Hall, pp. 131–148.CrossRefGoogle Scholar
, , and (2001). Stress proteins: a role in diapause? In Insect Timing: Circadian Rhythmicity to Seasonality, ed. , , and Amsterdam: Elsevier Science, pp. 155–171.CrossRefGoogle Scholar
, , , , , and (1998). The effect of cold acclimation and deacclimation on cold tolerance, trehalose, and free amino acid levels in Sitophilus granaries and Cryptolestes ferrugineus (Coleoptera). Journal of Insect Physiology 44, 955–965.CrossRefGoogle Scholar
and (2007). p38 MAPK is a likely component of the signal transduction pathway triggering rapid cold hardening in the flesh fly Sarcophaga crassipalpis. Journal of Experimental Biology 210, 3295–3300.CrossRefGoogle ScholarPubMed
, , , and (1998). Factors affecting carbohydrate and free amino acid content in overwintering larvae of Enosima leucotaeniella. Journal of Insect Physiology 44, 87–94.CrossRefGoogle Scholar
, , , , and (2001). Relationships between cold hardiness and diapause, and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer, Ostrinia furncalis. Journal of Insect Physiology 47, 157–165.CrossRefGoogle Scholar
(2001a). Expression of Drosophila homologue of senescence marker protein-30 during cold acclimation. Journal of Insect Physiology 46, 1111–1120.CrossRefGoogle Scholar
(2001b). A novel gene that is up-regulated during recovery from cold shock in Drosophila melanogaster. Gene 270, 259–264.CrossRefGoogle ScholarPubMed
, , , , and (2005). Temporal expression patterns of diapause-associated genes in flesh fly pupae from the onset of diapause through post-diapause quiescence. Journal of Insect Physiology 51, 631–640.CrossRefGoogle ScholarPubMed
and (1993). Purification and characterization of glycogen phosphorylase A and B from the freeze-avoiding gall moth larvae Epiblema scudderiana. Journal of Comparative Physiology B 163, 499–507.Google Scholar
, , and (1990). Cold shock elicits expression of heat shock proteins in the flesh fly, Sarcophaga crassipalpis. Journal of Insect Physiology 36, 825–834.CrossRefGoogle Scholar
, , , , , , and (2007). Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content. Plant Journal 50, 967–981.CrossRefGoogle ScholarPubMed
and (2001). Rapid cold-hardening of Drosophila melanogaster (Diptera: Drosophilidae) during ecologically-based thermoperiodic cycles. Journal of Experimental Biology 204, 1659–1666.Google ScholarPubMed
, , , and (2006). Up-regulation of two actin genes and redistribution of actin during diapause and cold stress in the northern house mosquito, Culex pipiens. Journal of Insect Physiology 52, 1226–1233.CrossRefGoogle ScholarPubMed
, , , and (2007). Multiple component system of sugars and polyols in the overwintering spruce bark beetle, Ips typographus. Journal of Insect Physiology 53, 581–586.CrossRefGoogle ScholarPubMed
and (2008). The 70kDa heat shock protein assists during the reparation of chilling injury in the insect, Pyrrhocoris apterus. Journal of Insect Physiology 53, 581–586.Google Scholar
, , and (1987). A rapid cold-hardening process in insects. Science 238, 1415–1417.CrossRefGoogle ScholarPubMed
, , and (2003). Variations in mitochondrial DNA and gene transcription in freezing-tolerant larvae of Eurosta solidaginis (Diptera: Tephritidae) and Gynaephora groenlandica (Lepidoptera: Lymantriidae). Insect Molecular Biology 12, 281–289.CrossRefGoogle Scholar
, , , and (2007). Proteomics of the flesh fly brain reveals an abundance of up-regulated heat shock proteins during pupal diapause. Journal of Insect Physiology 53, 385–391.CrossRefGoogle Scholar
and (2008). Rapid cold hardening elicits changes in the brain protein profiles of the flesh fly, Sarcophaga crassipalpis. Insect Molecular Biology, 17, 565–572.CrossRefGoogle ScholarPubMed
, , and (2002). Enzyme activities in overwintering larvae of the shonai ecotype of the rice stem borer, Chilo suppressalis Walker. Archives of Insect Biochemistry and Physiology 50, 53–61.CrossRefGoogle ScholarPubMed
and (2007). Shifts in the carbohydrate, polyol and amino acid pools during rapid cold-hardening and diapause-associated cold-hardening in flesh flies (Sarcophaga crassipalpis): a metabolomic comparison. Journal of Comparative Physiology B 177, 753–763.CrossRefGoogle ScholarPubMed
, , , , , and (2008). Metabolomics reveals unique and shared metabolic changes in response to heat shock, freezing, and desiccation in the Antarctic midge, Belgica antarctica. Journal of Insect Physiology 54, 645–655.CrossRefGoogle Scholar
and , (1997). Reversible phosphorylation of fructose 1,6-bisphosphatase mediates enzyme role in glycerol metabolism in the freeze-avoiding gall moth Epiblema scudderiana. Insect Biochemistry and Molecular Biology 27, 617–623.CrossRefGoogle Scholar
, , , , , and (2005). Metabolomic profiling of heat stress: hardening and recovery of homeostasis in Drosophila. American Journal of Physiology – Regulatory, Integrative, and Comparative Physiology 291, R205–R212.Google Scholar
, , , , , , and (2007). Metabolomic profiling of rapid cold hardening and cold shock in Drosophila melanogaster. Journal of Insect Physiology 53, 1218–1232.CrossRefGoogle ScholarPubMed
and (1979). The effect of exposure to low temperature on the metabolism of carbohydrates, lipids and protein in the larvae of Philosamia ricini. Journal of Biosciences 1, 441–446.CrossRefGoogle Scholar
, , , , and (2005). Cold hardening and transcriptional change in Drosophila melanogaster. Insect Molecular Biology 14, 607–613.CrossRefGoogle ScholarPubMed
and (2000). Heat-shock protein 90 is down-regulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress. Insect Molecular Biology 9, 641–645.CrossRefGoogle ScholarPubMed
, , and (2000). Developmental upregulation of inducible hsp70 transcripts, but not the cognate form, during pupal diapause in the flesh fly, Sarcophaga crassipalpis. Insect Biochemistry and Molecular Biology 30, 515–521.CrossRefGoogle ScholarPubMed
, , , , , and (2007). Up-regulation of heat shock proteins is essential for cold survival during insect diapause. Proceedings of the National Academy of Sciences, USA 104, 11130–11137.CrossRefGoogle ScholarPubMed
and (1994). Redirection of metabolism in the flesh fly, Sarcophaga bullata, following envenomation by the ectoparasitoid Nasonia vitripennis and correlation of metabolic effects with diapause status of the host. Journal of Insect Physiology 40, 207–215.CrossRefGoogle Scholar
, , , and (2007). Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization. Journal of Insect Physiology 53, 235–245.CrossRefGoogle ScholarPubMed
, , and (2007). Gene transcription during exposure to, and recovery from, cold and desiccation stress in Drososphila melanogaster. Insect Molecular Biology 16, 435–443.CrossRefGoogle Scholar
and (1992). Infradian cycles of oxygen consumption in dispausing pupae of the flesh fly, Sarcophaga crassipalpis, monitored by scanning microrespirographic method. Archives of Insect Biochemistry and Physiology 20, 135–143.CrossRefGoogle ScholarPubMed
, , , , and (2006). Cloning of heat shock protein genes (hsp90 and hsc70) and their expression during larval diapause and cold tolerance acquisition in the rice stem borer, Chilo suppressalis Walker. Archives of Insect Biochemistry and Physiology 63, 36–47.CrossRefGoogle ScholarPubMed
and (1981). Biochemical strategies of overwintering in the gall fly larva, Eurosta solidiginis: effect of low temperature acclimation on the activities of enzymes of intermediary metabolism. Journal of Comparative Physiology B 144, 191–199.CrossRefGoogle Scholar
and (1986). Freeze tolerant frogs' cryoprotectants and tissue metabolism during freeze-thaw cycles. Canadian Journal of Zoology 64, 49–56.CrossRefGoogle Scholar
and (1990). Carbon balance and energetics of cryoprotectant synthesis in a freeze-tolerant insect: responses to perturbation by anoxia. Journal of Comparative Physiology B 160, 77–84.CrossRefGoogle Scholar
and (1995). Metabolic responses to anoxia and freezing by the freeze tolerant marine mussel Geukensia demissus. Journal of Experimental Marine Biology and Ecology 188, 99–114.CrossRefGoogle Scholar
and (2004). Insect cold hardiness: new advances using gene-screening technology. In Life in the Cold: Evolution, Mechanisms, Adaptation, and Application, ed. , , Biological Papers of the University of Alaska, number 27. Fairbanks, Alaska, USA. pp. 275–281.Google Scholar
(1985). Domain structure and evolution in alpha-crystallins and small heat shock proteins. FEBS Letters 181, 1–6.CrossRefGoogle ScholarPubMed
(2000). Differential expression of two HSP70 transcripts in response to cold shock, thermoperiod, and adult diapause in the Colorado potato beetle. Journal of Insect Physiology 47, 1139–1145.CrossRefGoogle Scholar
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