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Chapter 16 - Infection, Inflammation, and Immunological Causes of Male Infertility

from Section 2 - Clinical Evaluation of the Infertile Male

Published online by Cambridge University Press:  08 July 2023

Larry I. Lipshultz
Affiliation:
Baylor College of Medicine, Texas
Stuart S. Howards
Affiliation:
University of Virginia
Craig S. Niederberger
Affiliation:
University of Illinois, Chicago
Dolores J. Lamb
Affiliation:
Weill Cornell Medical College, New York
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Summary

Infection and inflammation of the male reproductive tract are complex clinical conditions that can impact reproductive potential through a variety of pathophysiologic mechanisms. In this chapter, we discuss sites of genitourinary (GU) tract infections, infectious organisms, and the numerous ways in which leukocytes may impair male reproduction. Clinically, these processes most likely manifest as leukocytospermia and bacteriospermia.

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Publisher: Cambridge University Press
Print publication year: 2023

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References

Further Reading

Agarwal, A, Rana, M, Qiu, E, AlBunni, H, Bui, AD, Henkel, R. Role of oxidative stress, infection and inflammation in male infertility. Andrologia 2018;50:e13126.Google Scholar
Bachir, BG, Jarvi, K. Infectious, inflammatory, and immunologic conditions resulting in male infertility. Urol Clin North Am 2014;41:6781.Google Scholar
Gimenes, F, Souza, RP, Bento, JC, et al. Male infertility: a public health issue caused by sexually transmitted pathogens. Nat Rev Urol 2014;11:672–87.Google Scholar
Gregory, M, Cyr, DG. The blood–epididymis barrier and inflammation. Spermatogenesis 2014;4:e979619.Google Scholar
Wolff, H. The biologic significance of white blood cells in semen. Fertil Steril 1995;63:1143–57.Google Scholar

References

Brunner, RJ, Demeter, JH, Sindhwani, P. Review of guidelines for the evaluation and treatment of leukocytospermia in male infertility. World J Mens Health 2019;37:128–37.Google Scholar
Gdoura, R, Kchaou, W, Znazen, A, Chakroun, N, Fourati, M, Ammar-Keskes, L, et al. Screening for bacterial pathogens in semen samples from infertile men with and without leukocytospermia. Andrologia 2008;40:209–18.Google Scholar
Sergerie, M, Mieusset, R, Croute, F, Daudin, M, Bujan, L. High risk of temporary alteration of semen parameters after recent acute febrile illness. Fertil Steril 2007;88:970.e1–7.Google Scholar
Evenson, DP, Jost, LK, Corzett, M, Balhorn, R. Characteristics of human sperm chromatin structure following an episode of influenza and high fever: a case study. J Androl 2000;21:739–46.Google Scholar
Hærvig, KK, Kierkegaard, L, Lund, R, Bruunsgaard, H, Osler, M, Schmidt, L. Is male factor infertility associated with midlife low-grade inflammation? A population based study. Hum Fertil (Camb) 2018;21:146–54.Google Scholar
Smith, KB, Smith, MS. Obesity statistics. Prim Care 2016;43:121–35, ix.Google Scholar
Eisenberg, ML, Kim, S, Chen, Z, Sundaram, R, Schisterman, EF, Buck Louis, GM. The relationship between male BMI and waist circumference on semen quality: data from the LIFE study. Hum Reprod 2014;29:193200.Google Scholar
Eisenberg, ML, Li, S, Behr, B, Pera, RR, Cullen, MR. Relationship between semen production and medical comorbidity. Fertil Steril 2015;103:6671.Google Scholar
Lamm, S, Chidakel, A, Bansal, R. Obesity and hypogonadism. Urol Clin North Am 2016;43:239–45.Google Scholar
Morelli, A, Vignozzi, L, Maggi, M. Hypogonadotropic hypogonadism and metabolic syndrome: insights from the high-fat diet experimental rabbit animal model. Minerva Endocrinol 2016;41:240–9.Google Scholar
Phillips, KP, Tanphaichitr, N. Mechanisms of obesity-induced male infertility. Expert Rev Endocrinol Metab 2010;5:229–51.Google Scholar
Pilatz, A, Hudemann, C, Wolf, J, et al. Metabolic syndrome and the seminal cytokine network in morbidly obese males. Andrology 2017;5:2330.Google Scholar
Leisegang, K, Henkel, R, Agarwal, A. Obesity and metabolic syndrome associated systemic inflammation and the impact on the male reproductive system. Am J Reprod Immunol 2019;82:e13178.Google Scholar
Kahn, BE, Brannigan, RE. Obesity and male infertility. Curr Opin Urol 2017;27:441–5.Google Scholar
La Cava, A. Leptin in inflammation and autoimmunity. Cytokine 2017;98:51–8.Google Scholar
Leisegang, K, Bouic, PJD, Menkveld, R, Henkel, RR. Obesity is associated with increased seminal insulin and leptin alongside reduced fertility parameters in a controlled male cohort. Reprod Biol Endocrinol 2014;12:34.Google Scholar
Leisegang, K, Bouic, PJD, Henkel, RR. Metabolic syndrome is associated with increased seminal inflammatory cytokines and reproductive dysfunction in a case-controlled male cohort. Am J Reprod Immunol 2016;76:155–63.Google Scholar
Lotti, F, Corona, G, Cocci, A, et al. The prevalence of midline prostatic cysts and the relationship between cyst size and semen parameters among infertile and fertile men. Hum Reprod 2018;33:2023–34.Google Scholar
Yucel, C, Keskin, MZ, Cakmak, O, et al. Predictive value of pre-operative inflammation-based prognostic scores (neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-eosinophil ratio) in testicular sperm extraction: a pilot study. Andrology 2017;5:1100–4.Google Scholar
Cito, G, Coccia, ME, Picone, R, et al. Male inflammatory parameters are not useful to predict the outcomes of intracytoplasmic sperm injection: results from a cross-sectional study. World J Mens Health 2019;37:347–54.Google Scholar
Bar-Chama, N, Goluboff, E, Fisch, H. Infection and pyospermia in male infertility. Is it really a problem? Urol Clin North Am 1994;21:469–75.Google Scholar
Stanton, PG. Regulation of the blood–testis barrier. Semin Cell Dev Biol 2016;59:166–73.Google Scholar
Ainsworth, C. Microbiome: a bag of surprises. Nature 2017;551:S40–1.Google Scholar
Wolfe, AJ, Toh, E, Shibata, N, et al. Evidence of uncultivated bacteria in the adult female bladder. J Clin Microbiol 2012;50:1376–83.Google Scholar
Keck, C, Gerber-Schäfer, C, Clad, A, Wilhelm, C, Breckwoldt, M. Seminal tract infections: impact on male fertility and treatment options. Hum Reprod Update 1998;4:891903.Google Scholar
Syriou, V, Papanikolaou, D, Kozyraki, A, Goulis, DG. Cytokines and male infertility. Eur Cytokine Netw 2018;29:7382.Google Scholar
Comhaire, F, Verschraegen, G, Vermeulen, L. Diagnosis of accessory gland infection and its possible role in male infertility. Int J Androl 1980;3:3245.Google Scholar
Lackner, JE, Herwig, R, Schmidbauer, J, Schatzl, G, Kratzik, C, Marberger, M. Correlation of leukocytospermia with clinical infection and the positive effect of antiinflammatory treatment on semen quality. Fertil Steril 2006;86:601–5.Google Scholar
Jue, JS, Ramasamy, R. Significance of positive semen culture in relation to male infertility and the assisted reproductive technology process. Transl Androl Urol 2017;6:916–22.Google Scholar
Cottell, E, Harrison, RF, McCaffrey, M, Walsh, T, Mallon, E, Barry-Kinsella, C. Are seminal fluid microorganisms of significance or merely contaminants? Fertil Steril 2000;74:465–70.Google Scholar
Weidner, W, Pilatz, A, Diemer, T, Schuppe, HC, Rusz, A, Wagenlehner, F. Male urogenital infections: impact of infection and inflammation on ejaculate parameters. World J Urol 2013;31:717–23.Google Scholar
Trum, JW, Mol, BW, Pannekoek, Y, et al. Value of detecting leukocytospermia in the diagnosis of genital tract infection in subfertile men. Fertil Steril 1998;70:315–19.Google Scholar
Weng, S-L, Chiu, C-M, Lin, F-M, et al. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS ONE 2014;9:e110152.Google Scholar
Bachmann, LH, Manhart, LE, Martin, DH, et al. Advances in the understanding and treatment of male urethritis. Clin Infect Dis 2015;61 Suppl 8:S763–9.Google Scholar
Gottesman, T, Yossepowitch, O, Samra, Z, Rosenberg, S, Dan, M. Prevalence of Mycoplasma genitalium in men with urethritis and in high risk asymptomatic males in Tel Aviv: a prospective study. Int J STD AIDS 2017;28:127–32.Google Scholar
Cox, C, McKenna, JP, Watt, AP, Coyle, PV. Ureaplasma parvum and Mycoplasma genitalium are found to be significantly associated with microscopy-confirmed urethritis in a routine genitourinary medicine setting. Int J STD AIDS 2016;27:861–7.Google Scholar
Schiefer, HG. Microbiology of male urethroadnexitis: diagnostic procedures and criteria for aetiologic classification. Andrologia 1998;30 Suppl 1:713.Google Scholar
Bachir, BG, Jarvi, K. Infectious, inflammatory, and immunologic conditions resulting in male infertility. Urol Clin North Am 2014;41:6781.Google Scholar
Yeboah, ED, Marmar, JL. Urethral stricture and semen quality. Int J Fertil Menopausal Stud 1994;39:310–15.Google Scholar
Calhoun, EA, Meenan, RT, O’Keeffe Rosetti, MC, Gao, SY, Brown, S, Quentin Clemens, J. Prevalence of prostatitis-like symptoms in a managed care population. J Urol 2005;173(4S):910.Google Scholar
Naber, KG. Management of bacterial prostatitis: what’s new? BJU Int 2008;101 Suppl 3:710.Google Scholar
Drach, GW, Fair, WR, Meares, EM, Stamey, TA. Classification of benign diseases associated with prostatic pain: prostatitis or prostatodynia? J Urol 1978;120:266.Google Scholar
Nickel, JC, Shoskes, D, Wang, Y, et al. How does the pre-massage and post-massage 2-glass test compare to the Meares-Stamey 4-glass test in men with chronic prostatitis/chronic pelvic pain syndrome? J Urol 2006;176:119–24.Google Scholar
Krieger, JN, Nyberg, L, Nickel, JC. NIH consensus definition and classification of prostatitis. JAMA 1999;282:236–7.Google Scholar
Hua, VN, Schaeffer, AJ. Acute and chronic prostatitis. Med Clin North Am 2004;88:483–94.Google Scholar
Nickel, JC, Moon, T. Chronic bacterial prostatitis: an evolving clinical enigma. Urology 2005;66:28.Google Scholar
Nickel, JC, Downey, J, Johnston, B, Clark, J; Canadian Prostatitis Research Group. Predictors of patient response to antibiotic therapy for the chronic prostatitis/chronic pelvic pain syndrome: a prospective multicenter clinical trial. J Urol 2001;165:1539–44.Google Scholar
Cheah, PY, Liong, ML, Yuen, KH, et al. Initial, long-term, and durable responses to terazosin, placebo, or other therapies for chronic prostatitis/chronic pelvic pain syndrome. Urology 2004;64:881–6.Google Scholar
Yu, X, Jiang, D-S, Wang, J, et al. Fluoroquinolone use and the risk of collagen-associated adverse events: a systematic review and meta-analysis. Drug Saf 2019;42:1025–33.Google Scholar
Stephenson, AL, Wu, W, Cortes, D, Rochon, PA. Tendon injury and fluoroquinolone use: a systematic review. Drug Saf 2013;36:709–21.Google Scholar
Rosenstein, D, McAninch, JW. Urologic emergencies. Med Clin North Am 2004;88:495518.Google Scholar
Weidner, W, Krause, W, Ludwig, M. Relevance of male accessory gland infection for subsequent fertility with special focus on prostatitis. Hum Reprod Update 1999;5:421–32.Google Scholar
McConaghy, JR, Panchal, B. Epididymitis: an overview. Am Fam Physician 2016;94:723–6.Google Scholar
Haidl, G, Allam, JP, Schuppe, HC. Chronic epididymitis: impact on semen parameters and therapeutic options. Andrologia 2008;40:92–6.Google Scholar
Berger, RE, Alexander, ER, Harnisch, JP, et al. Etiology, manifestations and therapy of acute epididymitis: prospective study of 50 cases. J Urol 1979;121:750–4.Google Scholar
Weidner, W, Garbe, C, Weissbach, L, et al. [Initial therapy of acute unilateral epididymitis using ofloxacin. II. Andrological findings]. Urologe A 1990;29:277–80.Google Scholar
Dietz, O. [The change in the degree of fertility during the course of acute nonspecific epididymitis]. (Contribution to the pathogenesis of primary inhibition of spermiogenesis). Arch Klin Exp Dermatol 1960;211:160–6.Google Scholar
Osegbe, DN. Testicular function after unilateral bacterial epididymo-orchitis. Eur Urol 1991;19:204–8.Google Scholar
Rusz, A, Pilatz, A, Wagenlehner, F, et al. Influence of urogenital infections and inflammation on semen quality and male fertility. World J Urol 2012;30:2330.Google Scholar
Schuppe, H-C, Pilatz, A, Hossain, H, Diemer, T, Wagenlehner, F, Weidner, W. Urogenital infection as a risk factor for male infertility. Dtsch Arztebl Int 2017;114:339–46.Google Scholar
Stammler, A, Hau, T, Bhushan, S, et al. Epididymitis: ascending infection restricted by segmental boundaries. Hum Reprod 2015;30:1557–65.Google Scholar
Michel, V, Pilatz, A, Hedger, MP, Meinhardt, A. Epididymitis: revelations at the convergence of clinical and basic sciences. Asian J Androl 2015;17:756–63.Google Scholar
Dohle, GR. Inflammatory-associated obstructions of the male reproductive tract. Andrologia 2003;35:321–4.Google Scholar
Klein, B, Pant, S, Bhushan, S, et al. Dexamethasone improves therapeutic outcomes in a preclinical bacterial epididymitis mouse model. Hum Reprod 2019;34:1195–205.Google Scholar
Hviid, A, Rubin, S, Mühlemann, K. Mumps. Lancet 2008;371:932–44.Google Scholar
Burgher, SW. Acute scrotal pain. Emerg Med Clin North Am 1998;16:781809, vi.Google Scholar
Davis, NF, McGuire, BB, Mahon, JA, Smyth, AE, O’Malley, KJ, Fitzpatrick, JM. The increasing incidence of mumps orchitis: a comprehensive review. BJU Int 2010;105:1060–5.Google Scholar
Barták, V. Sperm count, morphology and motility after unilateral mumps orchitis. J Reprod Fertil 1973;32:491–4.Google Scholar
Lo, NC, Hotez, PJ. Public health and economic consequences of vaccine hesitancy for measles in the united states. JAMA Pediatr 2017;171:887–92.Google Scholar
Glasser, JW, Feng, Z, Omer, SB, Smith, PJ, Rodewald, LE. The effect of heterogeneity in uptake of the measles, mumps, and rubella vaccine on the potential for outbreaks of measles: a modelling study. Lancet Infect Dis 2016;16:599605.Google Scholar
Peltola, H, Heinonen, OP, Valle, M, et al. The elimination of indigenous measles, mumps, and rubella from Finland by a 12-year, two-dose vaccination program. N Engl J Med 1994;331:1397–402.Google Scholar
Marlow, MA, Marin, M, Moore, K, Patel, M. CDC guidance for use of a third dose of MMR vaccine during mumps outbreaks. J Public Health Manag Pract 2020;26:109–15.Google Scholar
Craighead, JE, Mahoney, EM, Carver, DH, Naficy, K, Fremont-Smith, P. Orchitis due to Coxsackie virus group B, type 5. Report of a case with isolation of virus from the testis. N Engl J Med 1962;267:498500.Google Scholar
Minzter, A. Orchitis as a complication of Coxsackie virus infection. J Med Soc N J 1963;60:463–6.Google Scholar
Freij, L, Norrby, R, Olsson, B. A small outbreak of Coxsackie B5 infection with two cases of cardiac involvement and orchitis followed by testicular atrophy. Acta Med Scand 1970;187:177–81.Google Scholar
Willems, WR, Hornig, C, Bauer, H, Klingmüller, V. A case of Coxsackie A9 virus infection with orchitis. J Med Virol 1978;3:137–40.Google Scholar
Willems, WR, Hornig, C, Bauer, H, Klingmüller, V. Orchitis caused by Coxsackie A9. Lancet 1977;2:1350.Google Scholar
Casella, R, Leibundgut, B, Lehmann, K, Gasser, TC. Mumps orchitis: report of a mini-epidemic. J Urol 1997;158:2158–61.Google Scholar
Ip, CCK, Tumali, K, Hoh, IM, Arunasalam, A. Acute epididymo-orchitis from Brucellosis melitensis in Australia. BMJ Case Rep 2019;12:e230007.Google Scholar
Agrawal, V, Ranjan, R. Scrotal abscess consequent on syphilitic epididymo-orchitis. Trop Doct 2019;49:45–7.Google Scholar
Cift, A, Yucel, MO. Comparison of inflammatory markers between Brucella and non-Brucella epididymo-orchitis. Int Braz J Urol 2018;44:771–8.Google Scholar
Street, EJ, Justice, ED, Kopa, Z, et al. The 2016 European guideline on the management of epididymo-orchitis. Int J STD AIDS. 2017;28:744–9.Google Scholar
Bosilkovski, M, Kamiloski, V, Miskova, S, Balalovski, D, Kotevska, V, Petrovski, M. Testicular infection in brucellosis: report of 34 cases. J Microbiol Immunol Infect 2018;51:82–7.Google Scholar
Naber, KG, Bergman, B, Bishop, MC, et al. EAU guidelines for the management of urinary and male genital tract infections. Urinary Tract Infection (UTI) Working Group of the Health Care Office (HCO) of the European Association of Urology (EAU). Eur Urol 2001;40:576–88.Google Scholar
Handsfield, HH, Lipman, TO, Harnisch, JP, Tronca, E, Holmes, KK. Asymptomatic gonorrhea in men. Diagnosis, natural course, prevalence and significance. N Engl J Med 1974;290:117–23.Google Scholar
Korenromp, EL, Sudaryo, MK, de Vlas, SJ, et al. What proportion of episodes of gonorrhoea and chlamydia becomes symptomatic? Int J STD AIDS 2002;13:91101.Google Scholar
Abusarah, EA, Awwad, ZM, Charvalos, E, Shehabi, AA. Molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males. Diagn Microbiol Infect Dis 2013;77:283–6.Google Scholar
Sellami, H, Znazen, A, Sellami, A, et al. Molecular detection of Chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in Tunisia: the effect on semen parameters and spermatozoa apoptosis markers. PLoS ONE 2014;9:e98903.Google Scholar
Senior, K. Chlamydia: a much underestimated STI. Lancet Infect Dis 2012;12:517–18.Google Scholar
Paavonen, J, Eggert-Kruse, W. Chlamydia trachomatis: impact on human reproduction. Hum Reprod Update 1999;5:433–47.Google Scholar
Cai, T, Pisano, F, Nesi, G, et al. Chlamydia trachomatis versus common uropathogens as a cause of chronic bacterial prostatitis: is there any difference? Results of a prospective parallel-cohort study. Investig Clin Urol 2017;58:460–7.Google Scholar
Mazzoli, S, Cai, T, Addonisio, P, Bechi, A, Mondaini, N, Bartoletti, R. Chlamydia trachomatis infection is related to poor semen quality in young prostatitis patients. Eur Urol 2010;57:708–14.Google Scholar
Karam, GH, Martin, DH, Flotte, TR, et al. Asymptomatic Chlamydia trachomatis infections among sexually active men. J Infect Dis 1986;154:900–3.Google Scholar
Rondeau, P, Valin, N, Decré, D, Girard, P-M, Lacombe, K, Surgers, L. Chlamydia trachomatis screening in urine among asymptomatic men attending an STI clinic in Paris: a cross-sectional study. BMC Infect Dis 2019;19:31.Google Scholar
Mackern-Oberti, JP, Motrich, RD, Breser, ML, Sánchez, LR, Cuffini, C, Rivero, VE. Chlamydia trachomatis infection of the male genital tract: an update. J Reprod Immunol 2013;100:3753.Google Scholar
Hosseinzadeh, S, Brewis, IA, Pacey, AA, Moore, HD, Eley, A. Coincubation of human spermatozoa with Chlamydia trachomatis in vitro causes increased tyrosine phosphorylation of sperm proteins. Infect Immun 2000;68:4872–6.Google Scholar
Hosseinzadeh, S, Brewis, IA, Eley, A, Pacey, AA. Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death. Hum Reprod 2001;16:293–9.Google Scholar
Hosseinzadeh, S, Pacey, AA, Eley, A. Chlamydia trachomatis-induced death of human spermatozoa is caused primarily by lipopolysaccharide. J Med Microbiol 2003;52(Pt 3):193200.Google Scholar
Eley, A, Hosseinzadeh, S, Hakimi, H, Geary, I, Pacey, AA. Apoptosis of ejaculated human sperm is induced by co-incubation with Chlamydia trachomatis lipopolysaccharide. Hum Reprod 2005;20:2601–7.Google Scholar
Eley, A, Pacey, AA, Galdiero, M, Galdiero, M, Galdiero, F. Can Chlamydia trachomatis directly damage your sperm? Lancet Infect Dis 2005;5:53–7.Google Scholar
Custo, GM, Lauro, V, Saitto, C, Frongillo, RF. Chlamydial infection and male infertility: an epidemiological study. Arch Androl 1989;23:243–8.Google Scholar
Wolff, H, Neubert, U, Zebhauser, M, Bezold, G, Korting, HC, Meurer, M. Chlamydia trachomatis induces an inflammatory response in the male genital tract and is associated with altered semen quality. Fertil Steril 1991;55:1017–19.Google Scholar
Cengiz, T, Aydoğanli, L, Baykam, M, et al. Chlamydial infections and male infertility. Int Urol Nephrol 1997;29:687–93.Google Scholar
Witkin, SS, Kligman, I, Bongiovanni, AM. Relationship between an asymptomatic male genital tract exposure to Chlamydia trachomatis and an autoimmune response to spermatozoa. Hum Reprod 1995;10:2952–5.Google Scholar
Munoz, MG, Witkin, SS. Autoimmunity to spermatozoa, asymptomatic Chlamydia trachomatis genital tract infection and gamma delta T lymphocytes in seminal fluid from the male partners of couples with unexplained infertility. Hum Reprod 1995;10:1070–4.Google Scholar
Moazenchi, M, Totonchi, M, Salman Yazdi, R, et al. The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: a comprehensive study. Int J STD AIDS 2018;29:466–73.Google Scholar
Cai, T, Wagenlehner, FME, Mondaini, N, et al. Effect of human papillomavirus and Chlamydia trachomatis co-infection on sperm quality in young heterosexual men with chronic prostatitis-related symptoms. BJU Int 2014;113:281–7.Google Scholar
Close, CE, Wang, SP, Roberts, PL, Berger, RE. The relationship of infection with Chlamydia trachomatis to the parameters of male fertility and sperm autoimmunity. Fertil Steril 1987;48:880–3.Google Scholar
Nagy, B, Corradi, G, Vajda, Z, Gimes, R, Csömör, S. The occurrence of Chlamydia trachomatis in the semen of men participating in an IVF programme. Hum Reprod 1989;4:54–6.Google Scholar
Ruijs, GJ, Kauer, FM, Jager, S, Schröder, PF, Schirm, J, Kremer, J. Is serology of any use when searching for correlations between Chlamydia trachomatis infection and male infertility? Fertil Steril 1990;53:131–6.Google Scholar
Soffer, Y, Ron-El, R, Golan, A, Herman, A, Caspi, E, Samra, Z. Male genital mycoplasmas and Chlamydia trachomatis culture: its relationship with accessory gland function, sperm quality, and autoimmunity. Fertil Steril 1990;53:331–6.Google Scholar
Bjercke, S, Purvis, K. Chlamydial serology in the investigation of infertility. Hum Reprod 1992;7:621–4.Google Scholar
Dieterle, S, Mahony, JB, Luinstra, KE, Stibbe, W. Chlamydial immunoglobulin IgG and IgA antibodies in serum and semen are not associated with the presence of Chlamydia trachomatis DNA or rRNA in semen from male partners of infertile couples. Hum Reprod 1995;10:315–19.Google Scholar
Eggert-Kruse, W, Buhlinger-Gopfarth, N, Rohr, G, et al. Antibodies to Chlamydia trachomatis in semen and relationship with parameters of male fertility. Hum Reprod 1996;11:1408–17.Google Scholar
Eggert-Kruse, W, Rohr, G, Demirakca, T, et al. Chlamydial serology in 1303 asymptomatic subfertile couples. Hum Reprod 1997;12:1464–75.Google Scholar
Habermann, B, Krause, W. Altered sperm function or sperm antibodies are not associated with chlamydial antibodies in infertile men with leucocytospermia. J Eur Acad Dermatol Venereol 1999;12:25–9.Google Scholar
Mackern-Oberti, JP, Motrich, RD, Breser, ML, et al. Male rodent genital tract infection with Chlamydia muridarum: persistence in the prostate gland that triggers self-immune reactions in genetically susceptible hosts. J Urol 2011;186:1100–6.Google Scholar
Motrich, RD, Sanchez, L, Maccioni, M, Mackern-Oberti, JP, Rivero, VE. Male rat genital tract infection with Chlamydia muridarum has no significant consequence on male fertility. J Urol 2012;187:1911–17.Google Scholar
Puerta Suarez, J, Sanchez, LR, Salazar, FC, et al. Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility. Sci Rep 2017;7:1126.Google Scholar
Dehghan Marvast, L, Talebi, AR, Ghasemzadeh, J, Hosseini, A, Pacey, AA. Effects of Chlamydia trachomatis infection on sperm chromatin condensation and DNA integrity. Andrologia 2018;50(3).Google Scholar
Eggert-Kruse, W, Rohr, G, Kunt, B, et al. Prevalence of Chlamydia trachomatis in subfertile couples. Fertil Steril 2003;80:660–3.Google Scholar
Hosseinzadeh, S, Eley, A, Pacey, AA. Semen quality of men with asymptomatic chlamydial infection. J Androl 2004;25:104–9.Google Scholar
Meyer, T. Diagnostic procedures to detect Chlamydia trachomatis infections. Microorganisms 2016;4:25.Google Scholar
Mårdh, PA, Colleen, S, Sylwan, J. Inhibitory effect on the formation of chlamydial inclusions in McCoy cells by seminal fluid and some of its components. Invest Urol 1980;17:510–13.Google Scholar
Moss, TR, Darougar, S, Woodland, RM, Nathan, M, Dines, RJ, Cathrine, V. Antibodies to Chlamydia species in patients attending a genitourinary clinic and the impact of antibodies to C. pneumoniae and C. psittaci on the sensitivity and the specificity of C. trachomatis serology tests. Sex Transm Dis 1993;20:61–5.Google Scholar
Gdoura, R, Kchaou, W, Chaari, C, et al. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis 2007;7:129.Google Scholar
Gdoura, R, Kchaou, W, Ammar-Keskes, L, et al. Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. J Androl 2008;29:198206.Google Scholar
Ondondo, RO, Whittington, WLH, Astete, SG, Totten, PA. Differential association of Ureaplasma species with non-gonococcal urethritis in heterosexual men. Sex Transm Infect 2010;86:271–5.Google Scholar
Ito, S, Hanaoka, N, Shimuta, K, et al. Male non-gonococcal urethritis: from microbiological etiologies to demographic and clinical features. Int J Urol 2016;23:325–31.Google Scholar
Gnarpe, H, Friberg, J. Mycoplasma and human reproductive failure. I. The occurrence of different mycoplasmas in couples with reproductive failure. Am J Obstet Gynecol 1972;114:727–31.Google Scholar
Swenson, CE, Toth, A, O’Leary, WM. Ureaplasma urealyticum and human infertility: the effect of antibiotic therapy on semen quality. Fertil Steril 1979;31:660–5.Google Scholar
Toth, A, Lesser, ML, Brooks, C, Labriola, D. Subsequent pregnancies among 161 couples treated for T-mycoplasma genital-tract infection. N Engl J Med 1983;308:505–7.Google Scholar
Fowlkes, DM, MacLeod, J, O’Leary, WM. T-mycoplasmas and human infertility: correlation of infection with alterations in seminal parameters. Fertil Steril 1975;26:1212–18.Google Scholar
Upadhyaya, M, Hibbard, BM, Walker, SM. The effect of Ureaplasma urealyticum on semen characteristics. Fertil Steril 1984;41:304–8.Google Scholar
Naessens, A, Foulon, W, Debrucker, P, Devroey, P, Lauwers, S. Recovery of microorganisms in semen and relationship to semen evaluation. Fertil Steril 1986;45:101–5.Google Scholar
Rose, BI, Scott, B. Sperm motility, morphology, hyperactivation, and ionophore-induced acrosome reactions after overnight incubation with mycoplasmas. Fertil Steril 1994;61:341–8.Google Scholar
Núñez-Calonge, R, Caballero, P, Redondo, C, Baquero, F, Martínez-Ferrer, M, Meseguer, MA. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod 1998;13:2756–61.Google Scholar
Reichart, M, Kahane, I, Bartoov, B. In vivo and in vitro impairment of human and ram sperm nuclear chromatin integrity by sexually transmitted Ureaplasma urealyticum infection. Biol Reprod 2000;63:1041–8.Google Scholar
Lee, JS, Kim, KT, Lee, HS, Yang, KM, Seo, JT, Choe, JH. Concordance of Ureaplasma urealyticum and Mycoplasma hominis in infertile couples: impact on semen parameters. Urology 2013;81:1219–24.Google Scholar
Liu, J, Wang, Q, Ji, X, et al. Prevalence of Ureaplasma urealyticum, Mycoplasma hominis, Chlamydia trachomatis infections, and semen quality in infertile and fertile men in China. Urology 2014;83:795–9.Google Scholar
Fowlkes, DM, Dooher, GB, O’Leary, WM. Evidence by scanning electron microscopy for an association between spermatozoa and T-mycoplasmas in men of infertile marriage. Fertil Steril 1975;26:1203–11.Google Scholar
Busolo, F, Zanchetta, R, Bertoloni, G. Mycoplasmic localization patterns on spermatozoa from infertile men. Fertil Steril 1984;42:412–17.Google Scholar
Busolo, F, Zanchetta, R. The effect of Mycoplasma hominis and Ureaplasma urealyticum on hamster egg in vitro penetration by human spermatozoa. Fertil Steril 1985;43:110–14.Google Scholar
Kalugdan, T, Chan, PJ, Seraj, IM, King, A. Polymerase chain reaction enzyme-linked immunosorbent assay detection of mycoplasma consensus gene in sperm with low oocyte penetration capacity. Fertil Steril 1996;66:793–7.Google Scholar
Potts, JM, Sharma, R, Pasqualotto, F, Nelson, D, Hall, G, Agarwal, A. Association of Ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. J Urol 2000;163:1775–8.Google Scholar
Ahmadi, MH, Mirsalehian, A, Sadighi Gilani, MA, Bahador, A, Talebi, M. Asymptomatic infection with Mycoplasma hominis negatively affects semen parameters and leads to male infertility as confirmed by improved semen parameters after antibiotic treatment. Urology 2017;100:97102.Google Scholar
Ahmadi, MH, Mirsalehian, A, Gilani, MAS, Bahador, A, Talebi, M. Improvement of semen parameters after antibiotic therapy in asymptomatic infertile men infected with Mycoplasma genitalium. Infection 2018;46:31–8.Google Scholar
de Louvois, J, Blades, M, Harrison, RF, Hurley, R, Stanley, VC. Frequency of mycoplasma in fertile and infertile couples. Lancet 1974;1:1073–5.Google Scholar
Harrison, RF, de Louvois, J, Blades, M, Hurley, R. Doxycycline treatment and human infertility. Lancet 1975;1:605–7.Google Scholar
de Jong, Z, Pontonnier, F, Plante, P, et al. Comparison of the incidence of Ureaplasma urealyticum in infertile men and in donors of semen. Eur Urol 1990;18:127–31.Google Scholar
Ombelet, W, Bosmans, E, Janssen, M, et al. Semen parameters in a fertile versus subfertile population: a need for change in the interpretation of semen testing. Hum Reprod 1997;12:987–93.Google Scholar
Al-Sweih, NA, Al-Fadli, AH, Omu, AE, Rotimi, VO. Prevalence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum infections and seminal quality in infertile and fertile men in Kuwait. J Androl 2012;33:1323–9.Google Scholar
Desai, S, Cohen, S, Khatamee, M, Leiter, E. Ureaplasma urealyticum (T-mycoplasma) infection: does it have a role in male infertility? J Urol 1980;124:469–71.Google Scholar
Cintron, RD, Wortham, JW, Acosta, A. The association of semen factors with the recovery of Ureaplasma urealyticum. Fertil Steril 1981;36:648–52.Google Scholar
Lewis, RW, Harrison, RM, Domingue, GJ. Culture of seminal fluid in a fertility clinic. Fertil Steril 1981;35:194–8.Google Scholar
Lumpkin, MM, Smith, TF, Coulam, CB, O’Brien, PC. Ureaplasma urealyticum in semen for artificial insemination: its effect on conception and semen analysis parameters. Int J Fertil 1987;32:122.Google Scholar
Shalhoub, D, Abdel-Latif, A, Fredericks, CM, Mathur, S, Rust, PF. Physiological integrity of human sperm in the presence of Ureaplasma urealyticum. Arch Androl 1986;16:7580.Google Scholar
Gerovassili, A, Marcandona, O, Asimakopoulos, B, Karavasilis, V, Panopoulou, M, Ikonomidis, A. Relationship between Chlamydia-Ureaplasma-Mycoplasma genital detection with semen concentration and motility among Greek men. Int J Fertil Steril 2017;11:130–3.Google Scholar
Kanakas, N, Mantzavinos, T, Boufidou, F, Koumentakou, I, Creatsas, G. Ureaplasma urealyticum in semen: is there any effect on in vitro fertilization outcome? Fertil Steril 1999;71:523–7.Google Scholar
Montagut, JM, Leprêtre, S, Degoy, J, Rousseau, M. Ureaplasma in semen and IVF. Hum Reprod 1991;6:727–9.Google Scholar
Moragianni, D, Dryllis, G, Andromidas, P, et al. Genital tract infection and associated factors affect the reproductive outcome in fertile females and females undergoing in vitro fertilization. Biomed Rep 2019;10:231–7.Google Scholar
Shalika, S, Dugan, K, Smith, RD, Padilla, SL. The effect of positive semen bacterial and Ureaplasma cultures on in-vitro fertilization success. Hum Reprod 1996;11:2789–92.Google Scholar
Levy, R, Layani-Milon, MP, Giscard D’Estaing, S, et al. Screening for Chlamydia trachomatis and Ureaplasma urealyticum infection in semen from asymptomatic male partners of infertile couples prior to in vitro fertilization. Int J Androl 1999;22:113–18.Google Scholar
Blanchard, A, Hentschel, J, Duffy, L, Baldus, K, Cassell, GH. Detection of Ureaplasma urealyticum by polymerase chain reaction in the urogenital tract of adults, in amniotic fluid, and in the respiratory tract of newborns. Clin Infect Dis 1993;17 Suppl 1:S148–53.Google Scholar
Stellrecht, KA, Woron, AM, Mishrik, NG, Venezia, RA. Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas. J Clin Microbiol 2004;42:1528–33.Google Scholar
Teng, K, Li, M, Yu, W, Li, H, Shen, D, Liu, D. Comparison of PCR with culture for detection of Ureaplasma urealyticum in clinical samples from patients with urogenital infections. J Clin Microbiol 1994;32:2232–4.Google Scholar
Povlsen, K, Jensen, JS, Lind, I. Detection of Ureaplasma urealyticum by PCR and biovar determination by liquid hybridization. J Clin Microbiol 1998;36:3211–16.Google Scholar
Frølund, M, Björnelius, E, Lidbrink, P, Ahrens, P, Jensen, JS. Comparison between culture and a multiplex quantitative real-time polymerase chain reaction assay detecting Ureaplasma urealyticum and U. parvum. PLoS ONE 2014;9:e102743.Google Scholar
Huang, C, Zhu, HL, Xu, KR, Wang, SY, Fan, LQ, Zhu, WB. Mycoplasma and ureaplasma infection and male infertility: a systematic review and meta-analysis. Andrology 2015;3:809–16.Google Scholar
Kasturi, SS, Osterberg, EC, Tannir, J, Brannigan, RE. The effect of genital tract infection and inflammation on male infertility. In: Lipshultz, LI, Howards, SS, Niederberger, CS, eds. Infertility in the Male, 4th ed. Cambridge: Cambridge University Press, 2009; pp. 295330.Google Scholar
Holmes, KK, Handsfield, HH, Wang, SP, et al. Etiology of nongonococcal urethritis. N Engl J Med 1975;292:1199–205.Google Scholar
Pellati, D, Mylonakis, I, Bertoloni, G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol 2008;140:311.Google Scholar
Fowler, JE, Mariano, M. Bacterial infection and male infertility: absence of immunoglobulin A with specificity for common Escherichia coli 0-serotypes in seminal fluid of infertile men. J Urol 1983;130:171–4.Google Scholar
Hillier, SL, Rabe, LK, Muller, CH, Zarutskie, P, Kuzan, FB, Stenchever, MA. Relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic. Obstet Gynecol 1990;75:800–4.Google Scholar
Merino, G, Carranza-Lira, S, Murrieta, S, Rodriguez, L, Cuevas, E, Morán, C. Bacterial infection and semen characteristics in infertile men. Arch Androl 1995;35:43–7.Google Scholar
Esfandiari, N, Saleh, RA, Abdoos, M, Rouzrokh, A, Nazemian, Z. Positive bacterial culture of semen from infertile men with asymptomatic leukocytospermia. Int J Fertil Womens Med 2002;47:265–70.Google Scholar
Teague, NS, Boyarsky, S, Glenn, JF. Interference of human spermatozoa motility by Escherichia coli. Fertil Steril 1971;22:281–5.Google Scholar
Wolff, H, Panhans, A, Stolz, W, Meurer, M. Adherence of Escherichia coli to sperm: a mannose mediated phenomenon leading to agglutination of sperm and E. coli. Fertil Steril 1993;60:154–8.Google Scholar
el-Mulla, KF, Köhn, FM, Dandal, M, et al. In vitro effect of Escherichia coli on human sperm acrosome reaction. Arch Androl 1996;37:73–8.Google Scholar
Diemer, T, Weidner, W, Michelmann, HW, Schiefer, HG, Rovan, E, Mayer, F. Influence of Escherichia coli on motility parameters of human spermatozoa in vitro. Int J Androl 1996;19:271–7.Google Scholar
Diemer, T, Huwe, P, Michelmann, HW, Mayer, F, Schiefer, HG, Weidner, W. Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis. Int J Androl 2000;23:178–86.Google Scholar
Schulz, M, Sánchez, R, Soto, L, Risopatrón, J, Villegas, J. Effect of Escherichia coli and its soluble factors on mitochondrial membrane potential, phosphatidylserine translocation, viability, and motility of human spermatozoa. Fertil Steril 2010;94:619–23.Google Scholar
Boguen, R, Treulen, F, Uribe, P, Villegas, JV. Ability of Escherichia coli to produce hemolysis leads to a greater pathogenic effect on human sperm. Fertil Steril 2015;103:1155–61.Google Scholar
Zeyad, A, Hamad, M, Amor, H, Hammadeh, ME. Relationships between bacteriospermia, DNA integrity, nuclear protamine alteration, sperm quality and ICSI outcome. Reprod Biol 2018;18:115–21.Google Scholar
Muzny, CA, Schwebke, JR. The clinical spectrum of Trichomonas vaginalis infection and challenges to management. Sex Transm Infect 2013;89:423–5.Google Scholar
Tuttle, JP, Holbrook, TW, Derrick, FC. Interference of human spermatozoal motility by Trichomonas vaginalis. J Urol 1977;118:1024–5.Google Scholar
Jarecki-Black, JC, Lushbaugh, WB, Golosov, L, Glassman, AB. Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor. Ann Clin Lab Sci 1988;18:484–9.Google Scholar
Gopalkrishnan, K, Hinduja, IN, Kumar, TC. Semen characteristics of asymptomatic males affected by Trichomonas vaginalis. J In Vitro Fert Embryo Transf 1990;7:165–7.Google Scholar
Lloyd, GL, Case, JR, De Frias, D, Brannigan, RE. Trichomonas vaginalis orchitis with associated severe oligoasthenoteratospermia and hypogonadism. J Urol 2003;170:924.Google Scholar
Gong, Y-H, Liu, Y, Li, P, et al. A nonobstructive azoospermic patient with Trichomonas vaginalis infection in testes. Asian J Androl 2018;20:97–8.Google Scholar
Benchimol, M, de Andrade Rosa, I, da Silva Fontes, R, Burla Dias, AJ. Trichomonas adhere and phagocytose sperm cells: adhesion seems to be a prominent stage during interaction. Parasitol Res 2008;102:597604.Google Scholar
Krause, W, Herbstreit, F, Slenzka, W. Are viral infections the cause of leukocytospermia? Andrologia 2002;34:8790.Google Scholar
Kapranos, N, Petrakou, E, Anastasiadou, C, Kotronias, D. Detection of herpes simplex virus, cytomegalovirus, and Epstein-Barr virus in the semen of men attending an infertility clinic. Fertil Steril 2003;79 Suppl 3:1566–70.Google Scholar
Yang, YS, Ho, HN, Chen, HF, et al. Cytomegalovirus infection and viral shedding in the genital tract of infertile couples. J Med Virol 1995;45:179–82.Google Scholar
Levy, R, Najioullah, F, Keppi, B, et al. Detection of cytomegalovirus in semen from a population of men seeking infertility evaluation. Fertil Steril 1997;68:820–5.Google Scholar
Naumenko, V, Tyulenev, Y, Kurilo, L, et al. Detection and quantification of human herpes viruses types 4–6 in sperm samples of patients with fertility disorders and chronic inflammatory urogenital tract diseases. Andrology 2014;2:687–94.Google Scholar
Bezold, G, Schuster-Grusser, A, Lange, M, Gall, H, Wolff, H, Peter, RU. Prevalence of human herpesvirus types 1–8 in the semen of infertility patients and correlation with semen parameters. Fertil Steril 2001;76:416–18.Google Scholar
Bezold, G, Politch, JA, Kiviat, NB, Kuypers, JM, Wolff, H, Anderson, DJ. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertil Steril 2007;87:1087–97.Google Scholar
Green, J, Monteiro, E, Bolton, VN, Sanders, P, Gibson, PE. Detection of human papillomavirus DNA by PCR in semen from patients with and without penile warts. Genitourin Med 1991;67:207–10.Google Scholar
Lai, YM, Lee, JF, Huang, HY, Soong, YK, Yang, FP, Pao, CC. The effect of human papillomavirus infection on sperm cell motility. Fertil Steril 1997;67:1152–5.Google Scholar
Luttmer, R, Dijkstra, MG, Snijders, PJF, et al. Presence of human papillomavirus in semen in relation to semen quality. Hum Reprod 2016;31:280–6.Google Scholar
Garolla, A, Engl, B, Pizzol, D, et al. Spontaneous fertility and in vitro fertilization outcome: new evidence of human papillomavirus sperm infection. Fertil Steril 2016;105:6572.e1.Google Scholar
Korhonen, C, Srinivasan, S, Huang, D, et al. Semen bacterial concentrations and HIV-1 RNA shedding among HIV-1-seropositive Kenyan men. J Acquir Immune Defic Syndr 2017;74:250–7.Google Scholar
Chéret, A, Durier, C, Mélard, A, et al. Impact of early cART on HIV blood and semen compartments at the time of primary infection. PLoS ONE 2017;12:e0180191.Google Scholar
Krieger, JN, Coombs, RW, Collier, AC, et al. Fertility parameters in men infected with human immunodeficiency virus. J Infect Dis 1991;164:464–9.Google Scholar
Crittenden, JA, Handelsman, DJ, Stewart, GJ. Semen analysis in human immunodeficiency virus infection. Fertil Steril 1992;57:1294–9.Google Scholar
Anderson, DJ, O’Brien, TR, Politch, JA, et al. Effects of disease stage and zidovudine therapy on the detection of human immunodeficiency virus type 1 in semen. JAMA 1992;267:2769–74.Google Scholar
Politch, JA, Mayer, KH, Abbott, AF, Anderson, DJ. The effects of disease progression and zidovudine therapy on semen quality in human immunodeficiency virus type 1 seropositive men. Fertil Steril 1994;61:922–8.Google Scholar
Dondero, F, Rossi, T, D’Offizi, G, et al. Semen analysis in HIV seropositive men and in subjects at high risk for HIV infection. Hum Reprod 1996;11:765–8.Google Scholar
Muller, CH, Coombs, RW, Krieger, JN. Effects of clinical stage and immunological status on semen analysis results in human immunodeficiency virus type 1-seropositive men. Andrologia 1998;30 Suppl 1:1522.Google Scholar
Nicopoullos, JDM, Almeida, PA, Ramsay, JWA, Gilling-Smith, C. The effect of human immunodeficiency virus on sperm parameters and the outcome of intrauterine insemination following sperm washing. Hum Reprod 2004;19:2289–97.Google Scholar
Dulioust, E, Du, AL, Costagliola, D, et al. Semen alterations in HIV-1 infected men. Hum Reprod 2002;17:2112–18.Google Scholar
Nicopoullos, JDM, Almeida, P, Vourliotis, M, Gilling-Smith, C. A decade of the sperm-washing programme: correlation between markers of HIV and seminal parameters. HIV Med 2011;12:195201.Google Scholar
Pavili, L, Daudin, M, Moinard, N, et al. Decrease of mitochondrial DNA level in sperm from patients infected with human immunodeficiency virus-1 linked to nucleoside analogue reverse transcriptase inhibitors. Fertil Steril 2010;94:2151–6.Google Scholar
Frapsauce, C, Grabar, S, Leruez-Ville, M, et al. Impaired sperm motility in HIV-infected men: an unexpected adverse effect of efavirenz? Hum Reprod 2015;30:1797–806.Google Scholar
Quayle, AJ, Xu, C, Mayer, KH, Anderson, DJ. T lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis 1997;176:960–8.Google Scholar
Kim, LU, Johnson, MR, Barton, S, et al. Evaluation of sperm washing as a potential method of reducing HIV transmission in HIV-discordant couples wishing to have children. AIDS 1999;13:645–51.Google Scholar
Semprini, AE, Levi-Setti, P, Bozzo, M, et al. Insemination of HIV-negative women with processed semen of HIV-positive partners. Lancet 1992;340:1317–19.Google Scholar
Marina, S, Marina, F, Alcolea, R, et al. Human immunodeficiency virus type 1 – serodiscordant couples can bear healthy children after undergoing intrauterine insemination. Fertil Steril 1998;70:35–9.Google Scholar
Persico, T, Savasi, V, Ferrazzi, E, Oneta, M, Semprini, AE, Simoni, G. Detection of human immunodeficiency virus-1 RNA and DNA by extractive and in situ PCR in unprocessed semen and seminal fractions isolated by semen-washing procedure. Hum Reprod 2006;21:1525–30.Google Scholar
Zafer, M, Horvath, H, Mmeje, O, et al. Effectiveness of semen washing to prevent human immunodeficiency virus (HIV) transmission and assist pregnancy in HIV-discordant couples: a systematic review and meta-analysis. Fertil Steril 2016;105:64555.e2.Google Scholar
Musso, D, Roche, C, Robin, E, Nhan, T, Teissier, A, Cao-Lormeau, V-M. Potential sexual transmission of Zika virus. Emerging Infect Dis 2015;21:359–61.Google Scholar
Oliveira, DBL, Durigon, GS, Mendes, ÉA, et al. Persistence and intra-host genetic evolution of Zika virus infection in symptomatic adults: a special view in the male reproductive system. Viruses 2018;10:615.Google Scholar
Rasmussen, SA, Jamieson, DJ, Honein, MA, Petersen, LR. Zika virus and birth defects – reviewing the evidence for causality. N Engl J Med 2016;374:1981–7.Google Scholar
Mansuy, JM, Suberbielle, E, Chapuy-Regaud, S, et al. Zika virus in semen and spermatozoa. Lancet Infect Dis 2016;16:1106–7.Google Scholar
Nicastri, E, Castilletti, C, Liuzzi, G, Iannetta, M, Capobianchi, MR, Ippolito, G. Persistent detection of Zika virus RNA in semen for six months after symptom onset in a traveller returning from Haiti to Italy, February 2016. Euro Surveill 2016;21:30314.Google Scholar
Huits, R, De Smet, B, Ariën, KK, Van Esbroeck, M, Bottieau, E, Cnops, L. Zika virus in semen: a prospective cohort study of symptomatic travellers returning to Belgium. Bull World Health Organ 2017;95:802–9.Google Scholar
Kurscheidt, FA, Mesquita, CSS, Damke, GMZF, et al. Persistence and clinical relevance of Zika virus in the male genital tract. Nat Rev Urol 2019;16:211–30.Google Scholar
Mead, PS, Duggal, NK, Hook, SA, et al. Zika virus shedding in semen of symptomatic infected men. N Engl J Med 2018;378:1377–85.Google Scholar
Joguet, G, Mansuy, J-M, Matusali, G, et al. Effect of acute Zika virus infection on sperm and virus clearance in body fluids: a prospective observational study. Lancet Infect Dis 2017;17:1200–8.Google Scholar
Borges, ED, Vireque, AA, Berteli, TS, Ferreira, CR, Silva, AS, Navarro, PA. An update on the aspects of Zika virus infection on male reproductive system. J Assist Reprod Genet 2019;36:1339–49.Google Scholar
Lackner, JE, Lakovic, E, Waldhör, T, Schatzl, G, Marberger, M. Spontaneous variation of leukocytospermia in asymptomatic infertile males. Fertil Steril 2008;90:1757–60.Google Scholar
el-Demiry, MI, Young, H, Elton, RA, Hargreave, TB, James, K, Chisholm, GD. Leucocytes in the ejaculate from fertile and infertile men. Br J Urol 1986;58:715–20.Google Scholar
Wolff, H, Anderson, DJ. Immunohistologic characterization and quantitation of leukocyte subpopulations in human semen. Fertil Steril 1988;49:497504.Google Scholar
Wolff, H, Politch, JA, Martinez, A, Haimovici, F, Hill, JA, Anderson, DJ. Leukocytospermia is associated with poor semen quality. Fertil Steril 1990;53:528–36.Google Scholar
Arata De Bellabarba, G, Tortoler, I. Nonsperm cells in human semen and their relationship with semen parameters. Arch Androl 2000;45:131–6.Google Scholar
Gonzales, GF, Kortebani, G, Mazzolli, AB. Leukocytospermia and function of the seminal vesicles on seminal quality. Fertil Steril 1992;57:1058–65.Google Scholar
Aziz, N, Agarwal, A, Lewis-Jones, I, Sharma, RK, Thomas, AJ. Novel associations between specific sperm morphological defects and leukocytospermia. Fertil Steril 2004;82:621–7.Google Scholar
Aitken, RJ, West, K, Buckingham, D. Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function. J Androl 1994;15:343–52.Google Scholar
Tomlinson, MJ, Barratt, CL, Cooke, ID. Prospective study of leukocytes and leukocyte subpopulations in semen suggests they are not a cause of male infertility. Fertil Steril 1993;60:1069–75.Google Scholar
el-Demiry, MI, Hargreave, TB, Busuttil, A, James, K, Chisholm, GD. Identifying leucocytes and leucocyte subpopulations in semen using monoclonal antibody probes. Urology 1986;28:492–6.Google Scholar
Yanushpolsky, EH, Politch, JA, Hill, JA, Anderson, DJ. Is leukocytospermia clinically relevant? Fertil Steril 1996;66:822–5.Google Scholar
Thomas, J, Fishel, SB, Hall, JA, Green, S, Newton, TA, Thornton, SJ. Increased polymorphonuclear granulocytes in seminal plasma in relation to sperm morphology. Hum Reprod 1997;12:2418–21.Google Scholar
Berger, RE, Karp, LE, Williamson, RA, Koehler, J, Moore, DE, Holmes, KK. The relationship of pyospermia and seminal fluid bacteriology to sperm function as reflected in the sperm penetration assay. Fertil Steril 1982;37:557–64.Google Scholar
Maruyama, DK, Hale, RW, Rogers, BJ. Effects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa. J Androl 1985;6:127–35.Google Scholar
Moubasher, A, Sayed, H, Mosaad, E, Mahmoud, A, Farag, F, Taha, EA. Impact of leukocytospermia on sperm dynamic motility parameters, DNA and chromosomal integrity. Cent European J Urol 2018;71:470–5.Google Scholar
Kaleli, S, Oçer, F, Irez, T, Budak, E, Aksu, MF. Does leukocytospermia associate with poor semen parameters and sperm functions in male infertility? The role of different seminal leukocyte concentrations. Eur J Obstet Gynecol Reprod Biol 2000;89:185–91.Google Scholar
Cavagna, M, Oliveira, JBA, Petersen, CG, et al. The influence of leukocytospermia on the outcomes of assisted reproductive technology. Reprod Biol Endocrinol 2012;10:44.Google Scholar
Lackner, JE, Märk, I, Sator, K, Huber, J, Sator, M. Effect of leukocytospermia on fertilization and pregnancy rates of artificial reproductive technologies. Fertil Steril 2008;90:869–71.Google Scholar
Ricci, G, Granzotto, M, Luppi, S, et al. Effect of seminal leukocytes on in vitro fertilization and intracytoplasmic sperm injection outcomes. Fertil Steril 2015;104:8793.Google Scholar
Castellini, C, D’Andrea, S, Martorella, A, et al. Relationship between leukocytospermia, reproductive potential after assisted reproductive technology, and sperm parameters: a systematic review and meta-analysis of case-control studies. Andrology 2020;8:125–35.Google Scholar
Aitken, RJ. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017;84:1039–52.Google Scholar
Bisht, S, Faiq, M, Tolahunase, M, Dada, R. Oxidative stress and male infertility. Nat Rev Urol 2017;14:470–85.Google Scholar
Aitken, RJ, Buckingham, DW, Brindle, J, Gomez, E, Baker, HW, Irvine, DS. Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma. Hum Reprod 1995;10:2061–71.Google Scholar
Iwasaki, A, Gagnon, C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992;57:409–16.Google Scholar
Gil-Guzman, E, Ollero, M, Lopez, MC, et al. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001;16:1922–30.Google Scholar
Ollero, M, Gil-Guzman, E, Lopez, MC, et al. Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod 2001;16:1912–21.Google Scholar
Krausz, C, Mills, C, Rogers, S, Tan, SL, Aitken, RJ. Stimulation of oxidant generation by human sperm suspensions using phorbol esters and formyl peptides: relationships with motility and fertilization in vitro. Fertil Steril 1994;62:599605.Google Scholar
Plante, M, de Lamirande, E, Gagnon, C. Reactive oxygen species released by activated neutrophils, but not by deficient spermatozoa, are sufficient to affect normal sperm motility. Fertil Steril 1994;62:387–93.Google Scholar
Richthoff, J, Elzanaty, S, Rylander, L, Hagmar, L, Giwercman, A. Association between tobacco exposure and reproductive parameters in adolescent males. Int J Androl 2008;31:31–9.Google Scholar
Dai, J-B, Wang, Z-X, Qiao, Z-D. The hazardous effects of tobacco smoking on male fertility. Asian J Androl 2015;17:954–60.Google Scholar
Asare-Anane, H, Bannison, SB, Ofori, EK, Ateko, RO, Bawah, AT, Amanquah, SD, et al. Tobacco smoking is associated with decreased semen quality. Reprod Health 2016;13:90.Google Scholar
Trummer, H, Habermann, H, Haas, J, Pummer, K. The impact of cigarette smoking on human semen parameters and hormones. Hum Reprod 2002;17:1554–9.Google Scholar
He, Y, Zou, L, Luo, W, et al. Heavy metal exposure, oxidative stress and semen quality: exploring associations and mediation effects in reproductive-aged men. Chemosphere 2020;244:125498.Google Scholar
Khushboo, M, Murthy, MK, Devi, MS, et al. Testicular toxicity and sperm quality following copper exposure in Wistar albino rats: ameliorative potentials of L-carnitine. Environ Sci Pollut Res Int 2018;25:1837–62.Google Scholar
Kaur, S, Saluja, M, Bansal, MP. Bisphenol A induced oxidative stress and apoptosis in mice testes: modulation by selenium. Andrologia 2018;50(3).Google Scholar
Ullah, A, Pirzada, M, Jahan, S, Ullah, H, Khan, MJ. Bisphenol A analogues bisphenol B, bisphenol F, and bisphenol S induce oxidative stress, disrupt daily sperm production, and damage DNA in rat spermatozoa: a comparative in vitro and in vivo study. Toxicol Ind Health 2019;35:294303.Google Scholar
de Lamirande, E, Leduc, BE, Iwasaki, A, Hassouna, M, Gagnon, C. Increased reactive oxygen species formation in semen of patients with spinal cord injury. Fertil Steril 1995;63:637–42.Google Scholar
Aitken, RJ, Clarkson, JS, Fishel, S. Generation of reactive oxygen species, lipid peroxidation, and human sperm function. Biol Reprod 1989;41:183–97.Google Scholar
Aitken, RJ, Buckingham, D, Harkiss, D. Use of a xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa. J Reprod Fertil 1993;97:441–50.Google Scholar
Alvarez, JG, Touchstone, JC, Blasco, L, Storey, BT. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl 1987;8:338–48.Google Scholar
Jones, R, Mann, T, Sherins, R. Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma. Fertil Steril 1979;31:531–7.Google Scholar
Aitken, RJ, Irvine, DS, Wu, FC. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am J Obstet Gynecol 1991;164:542–51.Google Scholar
Aitken, RJ. Impact of oxidative stress on male and female germ cells: implications for fertility. Reproduction. 2020;159:R189–201.Google Scholar
Mishra, S, Kumar, R, Malhotra, N, Singh, N, Dada, R. Mild oxidative stress is beneficial for sperm telomere length maintenance. World J Methodol 2016;6:163–70.Google Scholar
Tahamtan, S, Tavalaee, M, Izadi, T, et al. Reduced sperm telomere length in individuals with varicocele is associated with reduced genomic integrity. Sci Rep 2019;9:4336.Google Scholar
Twigg, J, Fulton, N, Gomez, E, Irvine, DS, Aitken, RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod 1998;13:1429–36.Google Scholar
Alvarez, JG, Sharma, RK, Ollero, M, et al. Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril 2002;78:319–29.Google Scholar
Guz, J, Gackowski, D, Foksinski, M, et al. Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men. PLoS ONE 2013;8:e68490.Google Scholar
Kodama, H, Yamaguchi, R, Fukuda, J, Kasai, H, Tanaka, T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997;68:519–24.Google Scholar
Stahl, PJ, Cogan, C, Mehta, A, Bolyakov, A, Paduch, DA, Goldstein, M. Concordance among sperm deoxyribonucleic acid integrity assays and semen parameters. Fertil Steril 2015;104:5661.e1.Google Scholar
Erenpreiss, J, Hlevicka, S, Zalkalns, J, Erenpreisa, J. Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J Androl 2002;23:717–23.Google Scholar
Sharma, RK, Pasqualotto, AE, Nelson, DR, Thomas, AJ, Agarwal, A. Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic. J Androl 2001;22:575–83.Google Scholar
Saleh, RA, Agarwal, A, Kandirali, E, et al. Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril 2002;78:1215–24.Google Scholar
Agarwal, A, Sharma, RK, Nallella, KP, Thomas, AJ, Alvarez, JG, Sikka, SC. Reactive oxygen species as an independent marker of male factor infertility. Fertil Steril 2006;86:878–85.Google Scholar
Agarwal, A, Mulgund, A, Alshahrani, S, et al. Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Reprod Biol Endocrinol 2014;12:126.Google Scholar
Jerre, E, Bungum, M, Evenson, D, Giwercman, A. Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection. Fertil Steril 2019;112:4653.e2.Google Scholar
Malić Vončina, S, Golob, B, Ihan, A, Kopitar, AN, Kolbezen, M, Zorn, B. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertil Steril 2016;105:63744.e1.Google Scholar
Bareh, GM, Jacoby, E, Binkley, P, Chang, T-CA, Schenken, RS, Robinson, RD. Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study. Fertil Steril 2016;105:32936.e1.Google Scholar
Wu, H, Whitcomb, BW, Huffman, A, et al. Associations of sperm mitochondrial DNA copy number and deletion rate with fertilization and embryo development in a clinical setting. Hum Reprod 2019;34:163–70.Google Scholar
McQueen, DB, Zhang, J, Robins, JC. Sperm DNA fragmentation and recurrent pregnancy loss: a systematic review and meta-analysis. Fertil Steril 2019;112:5460.e3.Google Scholar
Lewis, SE, Sterling, ES, Young, IS, Thompson, W. Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil Steril 1997;67:142–7.Google Scholar
Lewis, SE, Boyle, PM, McKinney, KA, Young, IS, Thompson, W. Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil Steril 1995;64:868–70.Google Scholar
Smith, R, Vantman, D, Ponce, J, Escobar, J, Lissi, E. Total antioxidant capacity of human seminal plasma. Hum Reprod 1996;11:1655–60.Google Scholar
Kovalski, NN, de Lamirande, E, Gagnon, C. Reactive oxygen species generated by human neutrophils inhibit sperm motility: protective effect of seminal plasma and scavengers. Fertil Steril 1992;58:809–16.Google Scholar
Otasevic, V, Stancic, A, Korac, A, Jankovic, A, Korac, B. Reactive oxygen, nitrogen, and sulfur species in human male fertility. A crossroad of cellular signaling and pathology. Biofactors 2020;46:206–19.Google Scholar
Hellstrom, WJ, Bell, M, Wang, R, Sikka, SC. Effect of sodium nitroprusside on sperm motility, viability, and lipid peroxidation. Fertil Steril 1994;61:1117–22.Google Scholar
Lewis, SE, Donnelly, ET, Sterling, ES, Kennedy, MS, Thompson, W, Chakravarthy, U. Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitric oxide is beneficial to sperm motility. Mol Hum Reprod 1996;2:873–8.Google Scholar
O’Bryan, MK, Zini, A, Cheng, CY, Schlegel, PN. Human sperm endothelial nitric oxide synthase expression: correlation with sperm motility. Fertil Steril 1998;70:1143–7.Google Scholar
Beckman, JS, Beckman, TW, Chen, J, Marshall, PA, Freeman, BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci U S A 1990;87:1620–4.Google Scholar
Weinberg, JB, Doty, E, Bonaventura, J, Haney, AF. Nitric oxide inhibition of human sperm motility. Fertil Steril 1995;64:408–13.Google Scholar
Rosselli, M, Dubey, RK, Imthurn, B, Macas, E, Keller, PJ. Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decreases sperm motility and induces sperm toxicity. Hum Reprod 1995;10:1786–90.Google Scholar
Nobunaga, T, Tokugawa, Y, Hashimoto, K, et al. Elevated nitric oxide concentration in the seminal plasma of infertile males: nitric oxide inhibits sperm motility. Am J Reprod Immunol 1996;36:193–7.Google Scholar
Revelli, A, Bergandi, L, Massobrio, M, Lindblom, B, Bosia, A, Ghigo, D. The concentration of nitrite in seminal plasma does not correlate with sperm concentration, sperm motility, leukocytospermia, or sperm culture. Fertil Steril 2001;76:496500.Google Scholar
Fuchs, TA, Abed, U, Goosmann, C, et al. Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 2007;176:231–41.Google Scholar
Wolff, H, Anderson, DJ. Evaluation of granulocyte elastase as a seminal plasma marker for leukocytospermia. Fertil Steril 1988;50:129–32.Google Scholar
Rajasekaran, M, Hellstrom, WJ, Naz, RK, Sikka, SC. Oxidative stress and interleukins in seminal plasma during leukocytospermia. Fertil Steril 1995;64:166–71.Google Scholar
Zorn, B, Virant-Klun, I, Meden-Vrtovec, H. Semen granulocyte elastase: its relevance for the diagnosis and prognosis of silent genital tract inflammation. Hum Reprod 2000;15:1978–84.Google Scholar
Henkel, R, Schill, WB. Sperm separation in patients with urogenital infections. Andrologia 1998;30 Suppl 1:91–7.Google Scholar
Zorn, B, Ihan, A, Kopitar, AN, Kolbezen, M, Sesek-Briski, A, Meden-Vrtovec, H. Changes in sperm apoptotic markers as related to seminal leukocytes and elastase. Reprod Biomed Online 2010;21:8492.Google Scholar
Maegawa, M, Kamada, M, Irahara, M, et al. A repertoire of cytokines in human seminal plasma. J Reprod Immunol 2002;54(1–2):3342.Google Scholar
Shimoya, K, Matsuzaki, N, Ida, N, et al. Detection of monocyte chemotactic and activating factor (MCAF) and interleukin (IL)-6 in human seminal plasma and effect of leukospermia on these cytokine levels. Am J Reprod Immunol 1995;34:311–16.Google Scholar
Omu, AE, Al-Qattan, F, Al-Abdul-Hadi, FM, Fatinikun, MT, Fernandes, S. Seminal immune response in infertile men with leukocytospermia: effect on antioxidant activity. Eur J Obstet Gynecol Reprod Biol 1999;86:195202.Google Scholar
Comhaire, F, Bosmans, E, Ombelet, W, Punjabi, U, Schoonjans, F. Cytokines in semen of normal men and of patients with andrological diseases. Am J Reprod Immunol 1994;31(2–3):99103.Google Scholar
Naz, RK, Kaplan, P. Increased levels of interleukin-6 in seminal plasma of infertile men. J Androl 1994;15:220–7.Google Scholar
Hill, JA, Haimovici, F, Politch, JA, Anderson, DJ. Effects of soluble products of activated lymphocytes and macrophages (lymphokines and monokines) on human sperm motion parameters. Fertil Steril 1987;47:460–5.Google Scholar
Eldamnhoury, EM, Elatrash, GA, Rashwan, HM, El-Sakka, AI. Association between leukocytospermia and semen interleukin-6 and tumor necrosis factor-alpha in infertile men. Andrology 2018;6:775–80.Google Scholar
Moretti, E, Collodel, G, Mazzi, L, Campagna, M, Iacoponi, F, Figura, N. Resistin, interleukin-6, tumor necrosis factor-alpha, and human semen parameters in the presence of leukocytospermia, smoking habit, and varicocele. Fertil Steril 2014;102:354–60.Google Scholar
Naz, RK, Evans, L, Armstrong, JS, Sikka, SC. Decreased levels of interleukin-12 are not correlated with leukocyte concentration and superoxide dismutase activity in semen of infertile men. Arch Androl 1998;41:91–6.Google Scholar
Huleihel, M, Lunenfeld, E, Horowitz, S, et al. Expression of IL-12, IL-10, PGE2, sIL-2R and sIL-6R in seminal plasma of fertile and infertile men. Andrologia 1999;31:283–8.Google Scholar
Ohta, S, Wada, H, Gabazza, EC, Nobori, T, Fuse, H. Evaluation of tissue factor antigen level in human seminal plasma. Urol Res 2002;30:317–20.Google Scholar
Rajasekaran, M, Hellstrom, W, Sikka, S. Quantitative assessment of cytokines (GRO alpha and IL-10) in human seminal plasma during genitourinary inflammation. Am J Reprod Immunol 1996;36:90–5.Google Scholar
Zeinali, M, Hadian Amree, A, Khorramdelazad, H, Karami, H, Abedinzadeh, M. Inflammatory and anti-inflammatory cytokines in the seminal plasma of infertile men suffering from varicocele. Andrologia 2017;49(6).Google Scholar
Hajizadeh Maleki, B, Tartibian, B. Resistance exercise modulates male factor infertility through anti-inflammatory and antioxidative mechanisms in infertile men: a RCT. Life Sci 2018;203:150–60.Google Scholar
Hajizadeh Maleki, B, Tartibian, B. High-intensity interval training modulates male factor infertility through anti-inflammatory and antioxidative mechanisms in infertile men: a randomized controlled trial. Cytokine 2020;125:154861.Google Scholar
Barbonetti, A, Castellini, C, D’Andrea, S, et al. Prevalence of anti-sperm antibodies and relationship of degree of sperm auto-immunization to semen parameters and post-coital test outcome: a retrospective analysis of over 10 000 men. Hum Reprod 2019;34:834–41.Google Scholar
Lu, S-M, Li, X, Wang, S-L, et al. Success rates of in vitro fertilization versus intracytoplasmic sperm injection in men with serum anti-sperm antibodies: a consecutive cohort study. Asian J Androl 2019;21:473–7.Google Scholar
Jiang, Y, Cui, D, Du, Y, et al. Association of anti-sperm antibodies with chronic prostatitis: a systematic review and meta-analysis. J Reprod Immunol 2016;118:8591.Google Scholar
Vazquez-Levin, MH, Marín-Briggiler, CI, Veaute, C. Antisperm antibodies: invaluable tools toward the identification of sperm proteins involved in fertilization. Am J Reprod Immunol 2014;72:206–18.Google Scholar
Shibahara, H, Shiraishi, Y, Hirano, Y, Suzuki, T, Takamizawa, S, Suzuki, M. Diversity of the inhibitory effects on fertilization by anti-sperm antibodies bound to the surface of ejaculated human sperm. Hum Reprod 2003;18:1469–73.Google Scholar
Bathla, H, Sidhu, KS. Effect of sperm-agglutinating antibodies on sperm capacitation and acrosome reaction. Int J Fertil Menopausal Stud 1996;41:528–33.Google Scholar
Fedder, J, Askjaer, SA, Hjort, T. Nonspermatozoal cells in semen: relationship to other semen parameters and fertility status of the couple. Arch Androl 1993;31:95103.Google Scholar
Kortebani, G, Gonzales, GF, Barrera, C, Mazzolli, AB. Leucocyte populations in semen and male accessory gland function: relationship with antisperm antibodies and seminal quality. Andrologia 1992;24:197204.Google Scholar
Gonzales, GF, Kortebani, G, Mazzolli, AB. Effect of isotypes of antisperm antibodies on semen quality. Int J Androl 1992;15:220–8.Google Scholar
Paschke, R, Schulze Bertelsbeck, D, Bahrs, S, Heinecke, A, Behre, HM. Seminal sperm antibodies exhibit an unstable spontaneous course and an increased incidence of leucocytospermia. Int J Androl 1994;17:135–9.Google Scholar
Eggert-Kruse, W, Neuer, A, Clussmann, C, et al. Seminal antibodies to human 60kd heat shock protein (HSP 60) in male partners of subfertile couples. Hum Reprod 2002;17:726–35.Google Scholar
Zini, A, Lefebvre, J, Kornitzer, G, et al. Anti-sperm antibody levels are not related to fertilization or pregnancy rates after IVF or IVF/ICSI. J Reprod Immunol 2011;88:80–4.Google Scholar
Zini, A, Fahmy, N, Belzile, E, Ciampi, A, Al-Hathal, N, Kotb, A. Antisperm antibodies are not associated with pregnancy rates after IVF and ICSI: systematic review and meta-analysis. Hum Reprod 2011;26:1288–95.Google Scholar
Trevor, C (ed). World Health Organization Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva: World Health Organization, 2010.Google Scholar
Eggert-Kruse, W, Bellmann, A, Rohr, G, Tilgen, W, Runnebaum, B. Differentiation of round cells in semen by means of monoclonal antibodies and relationship with male fertility. Fertil Steril 1992;58:1046–55.Google Scholar
Sigman, M, Lopes, L. The correlation between round cells and white blood cells in the semen. J Urol 1993;149(5 Pt 2):1338–40.Google Scholar
Couture, M, Ulstein, M, Leonard, J, Paulsen, CA. Improved staining method for differentiating immature germ cells from white blood cells in human seminal fluid. Andrologia 1976;8:61–6.Google Scholar
Wolff, H. The biologic significance of white blood cells in semen. Fertil Steril 1995;63:1143–57.Google Scholar
Williams, MA, Wick, A, Smith, DC. The influence of staining procedure on differential round cell analysis in stained smears of human semen. Biotech Histochem 1996;71:118–22.Google Scholar
Endtz, AW. A rapid staining method for differentiating granulocytes from “germinal cells” in Papanicolaou-stained semen. Acta Cytol 1974;18:27.Google Scholar
Politch, JA, Wolff, H, Hill, JA, Anderson, DJ. Comparison of methods to enumerate white blood cells in semen. Fertil Steril 1993;60:372–5.Google Scholar
Wolff, H. Methods for the detection of male genital tract inflammation. Andrologia 1998;30 Suppl 1:35–9.Google Scholar
Villegas, J, Schulz, M, Vallejos, V, Henkel, R, Miska, W, Sánchez, R. Indirect immunofluorescence using monoclonal antibodies for the detection of leukocytospermia: comparison with peroxidase staining. Andrologia 2002;34:6973.Google Scholar
Ricci, G, Presani, G, Guaschino, S, Simeone, R, Perticarari, S. Leukocyte detection in human semen using flow cytometry. Hum Reprod 2000;15:1329–37.Google Scholar
Jochum, M, Pabst, W, Schill, WB. Granulocyte elastase as a sensitive diagnostic parameter of silent male genital tract inflammation. Andrologia 1986;18:413–19.Google Scholar
Leino, L, Virkkunen, P. An automated chemiluminescence test for diagnosis of leukocytospermia. Int J Androl 1991;14:271–7.Google Scholar
Branigan, EF, Spadoni, LR, Muller, CH. Identification and treatment of leukocytospermia in couples with unexplained infertility. J Reprod Med 1995;40:625–9.Google Scholar
Yamamoto, M, Hibi, H, Katsuno, S, Miyake, K. Antibiotic and ejaculation treatments improve resolution rate of leukocytospermia in infertile men with prostatitis. Nagoya J Med Sci 1995;58(1–2):41–5.Google Scholar
Omu, AE, al-Othman, S, Mohamad, AS, al-Kaluwby, NM, Fernandes, S. Antibiotic therapy for seminal infection. Effect on antioxidant activity and T-helper cytokines. J Reprod Med 1998;43:857–64.Google Scholar
Yanushpolsky, EH, Politch, JA, Hill, JA, Anderson, DJ. Antibiotic therapy and leukocytospermia: a prospective, randomized, controlled study. Fertil Steril 1995;63:142–7.Google Scholar
Erel, CT, Sentürk, LM, Demir, F, Irez, T, Ertüngealp, E. Antibiotic therapy in men with leukocytospermia. Int J Fertil Womens Med 1997;42:206–10.Google Scholar
Schlegel, PN, Chang, TS, Marshall, FF. Antibiotics: potential hazards to male fertility. Fertil Steril 1991;55:235–42.Google Scholar
Hagan, S, Khurana, N, Chandra, S, et al. Differential expression of novel biomarkers (TLR-2, TLR-4, COX-2, and Nrf-2) of inflammation and oxidative stress in semen of leukocytospermia patients. Andrology 2015;3:848–55.Google Scholar
Gambera, L, Serafini, F, Morgante, G, Focarelli, R, De Leo, V, Piomboni, P. Sperm quality and pregnancy rate after COX-2 inhibitor therapy of infertile males with abacterial leukocytospermia. Hum Reprod 2007;22:1047–51.Google Scholar
Kavoussi, PK, Gilkey, MS, Hunn, C, et al. Ibuprofen does not have an adverse impact on semen parameters. J Assist Reprod Genet 2018;35:2201–4.Google Scholar
Vicari, E, La Vignera, S, Calogero, AE. Antioxidant treatment with carnitines is effective in infertile patients with prostatovesiculoepididymitis and elevated seminal leukocyte concentrations after treatment with nonsteroidal anti-inflammatory compounds. Fertil Steril 2002;78:1203–8.Google Scholar
Piomboni, P, Gambera, L, Serafini, F, Campanella, G, Morgante, G, De Leo, V. Sperm quality improvement after natural anti-oxidant treatment of asthenoteratospermic men with leukocytospermia. Asian J Androl 2008;10:201–6.Google Scholar
Oliva, A, Multigner, L. Ketotifen improves sperm motility and sperm morphology in male patients with leukocytospermia and unexplained infertility. Fertil Steril 2006;85:240–3.Google Scholar
Lasfargues, JE, Custis, D, Morrone, F, Carswell, J, Nguyen, T. A model for estimating spinal cord injury prevalence in the United States. Paraplegia 1995;33:62–8.Google Scholar
Brackett, NL, Nash, MS, Lynne, CM. Male fertility following spinal cord injury: facts and fiction. Phys Ther 1996;76:1221–31.Google Scholar
Brackett, NL, Santa-Cruz, C, Lynne, CM. Sperm from spinal cord injured men lose motility faster than sperm from normal men: the effect is exacerbated at body compared to room temperature. J Urol 1997;157:2150–3.Google Scholar
Linsenmeyer, TA, Perkash, I. Infertility in men with spinal cord injury. Arch Phys Med Rehabil 1991;72:747–54.Google Scholar
Aird, IA, Vince, GS, Bates, MD, Johnson, PM, Lewis-Jones, ID. Leukocytes in semen from men with spinal cord injuries. Fertil Steril 1999;72:97103.Google Scholar
Brackett, NL, Lynne, CM. The method of assisted ejaculation affects the outcome of semen quality studies in men with spinal cord injury: a review. NeuroRehabilitation 2000;15:89100.Google Scholar
Brackett, NL, Bloch, WE, Lynne, CM. Predictors of necrospermia in men with spinal cord injury. J Urol 1998;159:844–7.Google Scholar
Trabulsi, EJ, Shupp-Byrne, D, Sedor, J, Hirsch, IH. Leukocyte subtypes in electroejaculates of spinal cord injured men. Arch Phys Med Rehabil 2002;83:31–4.Google Scholar
Basu, S, Lynne, CM, Ruiz, P, Aballa, TC, Ferrell, SM, Brackett, NL. Cytofluorographic identification of activated T-cell subpopulations in the semen of men with spinal cord injuries. J Androl 2002;23:551–6.Google Scholar
Padron, OF, Brackett, NL, Sharma, RK, Lynne, CM, Thomas, AJ, Agarwal, A. Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertil Steril 1997;67:1115–20.Google Scholar
Vargas-Baquero, E, Johnston, S, Sánchez-Ramos, A, Arévalo-Martín, A, Wilson, R, Gosálvez, J. The incidence and etiology of sperm DNA fragmentation in the ejaculates of males with spinal cord injuries. Spinal Cord 2020;58:803–10.Google Scholar
Randall, JM, Evans, DH, Bird, VG, Aballa, TC, Lynne, CM, Brackett, NL. Leukocytospermia in spinal cord injured patients is not related to histological inflammatory changes in the prostate. J Urol 2003;170:897900.Google Scholar
Christiansen, E, Tollefsrud, A, Purvis, K. Sperm quality in men with chronic abacterial prostatovesiculitis verified by rectal ultrasonography. Urology 1991;38:545–9.Google Scholar
Leib, Z, Bartoov, B, Eltes, F, Servadio, C. Reduced semen quality caused by chronic abacterial prostatitis: an enigma or reality? Fertil Steril 1994;61:1109–16.Google Scholar
Krieger, JN, Berger, RE, Ross, SO, Rothman, I, Muller, CH. Seminal fluid findings in men with nonbacterial prostatitis and prostatodynia. J Androl 1996;17:310–18.Google Scholar
Weidner, W, Jantos, C, Schiefer, HG, Haidl, G, Friedrich, HJ. Semen parameters in men with and without proven chronic prostatitis. Arch Androl 1991;26:173–83.Google Scholar
Pasqualotto, FF, Sharma, RK, Potts, JM, Nelson, DR, Thomas, AJ, Agarwal, A. Seminal oxidative stress in patients with chronic prostatitis. Urology 2000;55:881–5.Google Scholar
Menkveld, R, Huwe, P, Ludwig, M, Weidner, W. Morphological sperm alternations in different types of prostatitis. Andrologia 2003;35:288–93.Google Scholar
Ludwig, M, Kümmel, C, Schroeder-Printzen, I, Ringert, RH, Weidner, W. Evaluation of seminal plasma parameters in patients with chronic prostatitis or leukocytospermia. Andrologia 1998;30 Suppl 1:41–7.Google Scholar
Motrich, RD, Maccioni, M, Molina, R, et al. Reduced semen quality in chronic prostatitis patients that have cellular autoimmune response to prostate antigens. Hum Reprod 2005;20:2567–72.Google Scholar
Fu, W, Zhou, Z, Liu, S, et al. The effect of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) on semen parameters in human males: a systematic review and meta-analysis. PLoS ONE 2014;9:e94991.Google Scholar
Condorelli, RA, Russo, GI, Calogero, AE, Morgia, G, La Vignera, S. Chronic prostatitis and its detrimental impact on sperm parameters: a systematic review and meta-analysis. J Endocrinol Invest 2017;40:1209–18.Google Scholar
Huang, C, Long, X, Jing, S, et al. Ureaplasma urealyticum and Mycoplasma hominis infections and semen quality in 19,098 infertile men in China. World J Urol 2016;34:1039–44.Google Scholar
Zhou, YH, Ma, HX, Shi, XX, Liu, Y. Ureaplasma spp. in male infertility and its relationship with semen quality and seminal plasma components. J Microbiol Immunol Infect 2018;51:778–83.Google Scholar
Peerayeh, SN, Yazdi, RS, Zeighami, H. Association of Ureaplasma urealyticum infection with varicocele-related infertility. J Infect Dev Ctries 2008;2:116–19.Google Scholar
Shen, L, Zhang, L, Zhang, X. [An analysis of CMV infection in 115 cases with viral hepatitis]. Zhonghua Yu Fang Yi Xue Za Zhi 1996;30:157–9.Google Scholar
Martorell, M, Gil-Salom, M, Pérez-Vallés, A, Garcia, JA, Rausell, N, Senpere, A. Presence of human papillomavirus DNA in testicular biopsies from nonobstructive azoospermic men. Arch Pathol Lab Med 2005;129:1132–6.Google Scholar
Behboudi, E, Mokhtari-Azad, T, Yavarian, J, et al. Molecular detection of HHV1–5, AAV and HPV in semen specimens and their impact on male fertility. Hum Fertil (Camb) 2019;22:133–8.Google Scholar
Bujan, L, Sergerie, M, Moinard, N, et al. Decreased semen volume and spermatozoa motility in HIV-1-infected patients under antiretroviral treatment. J Androl 2007;28:444–52.Google Scholar
Tomlinson, MJ, White, A, Barratt, CL, Bolton, AE, Cooke, ID. The removal of morphologically abnormal sperm forms by phagocytes: a positive role for seminal leukocytes? Hum Reprod 1992;7:517–22.Google Scholar
Kung, AW, Ho, PC, Wang, C. Seminal leucocyte subpopulations and sperm function in fertile and infertile Chinese men. Int J Androl 1993;16:189–94.Google Scholar
Wang, AW, Politch, J, Anderson, D. Leukocytospermia in male infertility patients in China. Andrologia 1994;26:167–72.Google Scholar
Homa, ST, Vassiliou, AM, Stone, J, et al. A Comparison between two assays for measuring seminal oxidative stress and their relationship with sperm DNA fragmentation and semen parameters. Genes (Basel) 2019;10:236.Google Scholar

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