Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting.

'This comprehensive book is welcome at this point of rapid growth in new technologies and discoveries such that keeping up with applications of genome engineering is challenging. Here we have both a general introduction to the field and to deep mechanisms and glimpses of future applications and ethical considerations - with gems for newcomers and experts alike.'

George M. Church - Harvard University, Massachusetts and Massachusetts Institute of Technology

'Krishnarao Appasani has assembled a comprehensive series of up-to-date chapters on endonuclease-based genomic engineering by experts and practitioners. The book presents a wide range of topics from basic science, applications to genetic screens and diseases, and legal and ethical issues. The many potential applications across a wide range of organisms are well illustrated. The book will become a must have for any researchers in academia and industry trying to keep up with this fast-moving field.'

Norbert Perrimon - Harvard Medical School, Boston

'… a remarkable book, which very successfully manages to provide deep insights into the various and latest aspects of gene editing and engineering. It is so far probably the best gene editing text collection available and highly suited to get a good understanding of the breadth and impact of this cutting-edge field of science.'

Rodger Novak - President, Director and Chair of the Board CRISPR Therapeutics AG, Switzerland

'… offers a widespread analysis of the state of the art concerning all the main tools available for modification of eukaryotic DNA in vivo, their application in metazoans and the societal issues involved. I thoroughly recommend it to anyone interested in gaining insight into this newest genetic revolution.'

Francisco Juan Martinez Mojica - Universidad de Alicante, Spain

'The field of precise genome engineering has been compiled into an excellent and essential guide through its landscape and it imparts state-of-the-art knowledge of its tools, its multiple research and clinical applications and related legal and ethical implications.'

Shamim Herbert Rahman - Senior Research Scientist, Novo Nordisk A/S, Måløv, Denmark

'… broadly covers recent development of genome editing from principle, history and usage to patent wars.'

Keiji Nishida - Kobe University, Japan

'… providing significant and useful information on current knowledge in the field and promoting the interaction between basic and pre-clinical/clinical research … of potential interest to a broad readership interested in basic and translational research.'

Antonio Russo - 'P. Giaccone' University Hospital, University of Palermo, Italy