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  • Print publication year: 2015
  • Online publication date: December 2015

6 - Multiple stressor effects on freshwater fish: a review and meta-analysis



A stressor can be defined as a variable that, as a result of human activity, exceeds its normal range of variation and affects individual species or communities (modified after Townsend et al., 2008). Most present-day ecosystems are exposed to multiple stressors acting simultaneously (Vinebrooke et al., 2004; Crain et al., 2008; Dudgeon, 2010) or sequentially (Christensen et al., 2006). Therefore, multiple stressors research is highly relevant for both fundamental and applied science, and when trying to deal with complex global problems. For example, multiple-stressor effects are believed to be responsible for the ongoing global declines of honeybees (reviews by Potts et al., 2010; Aebi & Neumann, 2011), amphibians (reviews by Sodhi et al., 2008; Mann et al., 2009; Hof et al., 2011), coral reefs (reviews by Harvey et al., 2013; McLeod et al., 2013) and freshwater biodiversity (reviews by Allan, 2004; Dudgeon, 2010; Ormerod et al., 2010; Vörösmarty et al., 2010; Woodward et al., 2010). Multiple-stressors effects are also a central concern when trying to understand and predict the all-pervasive impacts of global climate change (reviews by Lindenmayer et al., 2010; Woodward et al., 2010; Chmura et al., 2011; Hof et al., 2011; Harvey et al., 2013; McLeod et al., 2013).

The development of a general theory of multiple stressors has started relatively recently (Hay et al., 1994; Hay, 1996; Folt et al., 1999; Swanson, 2004; Vinebrooke et al., 2004) and is still ongoing (e.g. Crain et al., 2008; Downes, 2010; Statzner & Bêche, 2010; Boone et al., 2011; Verberk et al., 2013). The combined action of multiple stressors can produce either simple or complex responses. According to Folt et al. (1999), ‘simple’ additive responses are patterns where the effect of all stressors combined is equal to the sum of the effects of each individual stressor. By contrast, ‘complex’ outcomes can be synergistic or antagonistic, when the combined effect is either larger or smaller than one would expect based on the individual effects of each stressor.

From the viewpoint of a resource manager or conservation ecologist, complex outcomes of interacting multiple stressors are particularly important because they can lead to ‘ecological surprises’ (Paine et al., 1998; Lindenmayer et al., 2010). For example, due to synergistic interactions between stressors, the actual threat to a given endangered ecosystem may be far more severe than expected based on the combined knowledge from all single-stressor studies conducted to help protect this ecosystem.

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Aebi, A. & Neumann, P. (2011). Endosymbionts and honey bee colony losses?Trends in Ecology and Evolution, 26, 494–494.
Allan, J. D. (2004). Landscapes and riverscapes: the influence of land use on stream ecosystems. Annual Review of Ecology, Evolution and Systematics, 35, 257–284.
Awata, S., Tsuruta, T., Yada, T. & Iguchi, K. (2011). Effects of suspended sediment on cortisol levels in wild and cultured strains of ayu Plecoglossus altivelis. Aquaculture, 314, 115–121.
Banks, J. L. (1994). Raceway density and water-flows as factors affecting spring chinhook salmon (Oncorhynchus tshawytscha) during release and after release. Aquaculture, 119, 201–217.
Beard, T. D., Arlinghaus, R., Cooke, S. J., et al. (2011). Ecosystem approach to inland fisheries: research needs and implementation strategies. Biology Letters, 7, 481–483.
Bernhardt, E. S. & Palmer, M. A. (2011). River restoration: the fuzzy logic of repairing reaches to reverse catchment scale degradation. Ecological Applications, 21, 1926–1931.
Bernhardt, E. S., Palmer, M. A., Allan, J. D., et al. (2005). Synthesizing U.S. river restoration efforts. Science, 308, 636–637.
Blanar, C. A., Curtis, M. A. & Chan, H. M. (2005). Growth, nutritional composition, and hematology of Arctic charr (Salvelinus alpinus) exposed to toxaphene and tapeworm (Diphyllobothrium dendriticum) larvae. Archives of Environmental Contamination and Toxicology, 48, 397–404.
Boone, E., Ye, K. & Smith, E. (2011). Assessing environmental stressors via Bayesian model averaging in the presence of missing data. Environmetrics, 22, 13–22.
Bowen, L., Werner, I. & Johnson, M. L. (2006). Physiological and behavioral effects of zinc and temperature on coho salmon (Oncorhynchus kisutch). Hydrobiologia, 559, 161–168.
Brook, B. W., Sodhi, N. S. & Bradshaw, C. J. (2008). Synergies among extinction drivers under global change. Trends in Ecology & Evolution, 23, 453–460.
Brown, C. J., Saunders, M. I., Possingham, H. P. & Richardson, A. J. (2013). Managing for interactions between local and global stressors of ecosystems. PLoS ONE, 8, e65765.
Chmura, D. J., Anderson, P. D., Howe, G. T., et al. (2011). Forest responses to climate change in the northwestern United States: ecophysiological foundations for adaptive management. Forest Ecology and Management, 261, 1121–1142.
Christensen, M. R., Graham, M. D., Vinebrooke, R. D., et al. (2006). Multiple anthropogenic stressors cause ecological surprises in boreal lakes. Global Change Biology, 12, 2316–2322.
Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences. Seond edition. Hillsdale, NJ: Lawrence Erlbaum Associates.
Cottingham, K. L., Lennon, J. T. & Brown, B. L. (2005). Knowing when to draw the line: designing more informative ecological experiments. Frontiers in Ecology and the Environment, 3, 145–152.
Couillard, C. M., Courtenay, S. C. & Macdonald, R. W. (2008a). Chemical–environment interactions affecting the risk of impacts on aquatic organisms: a review with a Canadian perspective – interactions affecting vulnerability. Environmental Reviews, 16, 19–44.
Couillard, C. M., Macdonald, R. W., Courtenay, S. C. & Palace, V. P. (2008b). Chemical–environment interactions affecting the risk of impacts on aquatic organisms: a review with a Canadian perspective – interactions affecting exposure. Environmental Reviews, 16, 1–17.
Crain, C. M., Kroeker, K. & Halpern, B. S. (2008). Interactive and cumulative effects of multiple human stressors in marine systems. Ecology Letters, 11, 1304–1315.
Darling, E. S. & Côté, I. M. (2008). Quantifying the evidence for ecological synergies. Ecology Letters, 11, 1278–1286.
Downes, B. J. (2010). Back to the future: little used tools and principles of scientific inference can help disentangle effects of multiple stressors on freshwater ecosystems. Freshwater Biology, 55(S1), 60–79.
Drake, D. A. R. & Mandrak, N. E. (2014). Harvest models and stock co-occurrence: probabilistic methods for estimating bycatch. Fish and Fisheries, 15, 23–42.
Dudgeon, D. (2010). Prospects for sustaining freshwater biodiversity in the 21st century: linking ecosystem structure and function. Current Opinion in Environmental Sustainability, 2, 422–430.
Dudgeon, D., Arthington, A. H., Gessner, M. O., et al. (2006). Freshwater biodiversity: importance, threats, status and conservation challenges. Biological Reviews, 81, 163–182.
Ehlers, A., Worm, B. & Reusch, T. B. (2008). Importance of genetic diversity in eelgrass Zostera marina for its resilience to global warming. Marine Ecology Progress Series, 355, 1–7.
Erickson, R. J., Kleiner, C. F., Fiandt, J. T. & Highland, T. L. (1997). Effect of acclimation period on the relationship of acute copper toxicity to water hardness for fathead minnows. Environmental Toxicology and Chemistry, 16, 813–815.
Espinoza-Tenorio, A., Wolff, M., Taylor, M. H. & Espejel, I. (2012). What model suits ecosystem-based fisheries management? A plea for a structured modeling process. Reviews in Fish Biology and Fisheries, 22, 81–94.
European Commission. (2000). The EU Water Framework Directive – Integrated River Basin Management for Europe. Directive 2000/60/EC.
Everson, I., Taabu-Munyaho, A. & Kayanda, R. (2013). Acoustic estimates of commercial fish species in Lake Victoria: moving towards ecosystem-based fisheries management. Fisheries Research, 139, 65–75.
Fausch, K. D., Baxter, C. V. & Murakami, M. (2010). Multiple stressors in north temperate streams: lessons from linked foreststream ecosystems in northern Japan. Freshwater Biology, 55, 120–134.
Fogarty, M. J. & Rose, K. (2013). The art of ecosystem-based fishery management. Canadian Journal of Fisheries and Aquatic Sciences, 71, 479–490.
Folt, C. L., Chen, C. Y., Moore, M. V. & Burnaford, J. (1999). Synergism and antagonism among multiple stressors. Limnology and Oceanography, 44, 864–877.
Gonzalez, M. J., Knoll, L. B. & Vanni, M. J. (2010). Differential effects of elevated nutrient and sediment inputs on survival, growth and biomass of a common larval fish species (Dorosoma cepedianum). Freshwater Biology, 55, 654–669.
Grimm, N. B., Faeth, S. H., Golubiewski, N. E., et al. (2008). Global change and the ecology of cities. Science, 319, 756–760.
Gurevitch, J., Morrison, J. A. & Hedges, L. V. (2000). The interaction between competition and predation: a meta-analysis of field experiments. American Naturalist, 155, 435–453.
Haase, P., Hering, D., Jaehnig, S. C., Lorenz, A. W. & Sundermann, A. (2013). The impact of hydromorphological restoration on river ecological status: a comparison of fish, benthic invertebrates, and macrophytes. Hydrobiologia, 704, 475–488.
Häkkinen, J., Vehniainen, E. & Oikari, A. (2004). High sensitivity of northern pike larvae to UV-B but no UV-photoinduced toxicity of retene. Aquatic Toxicology, 66, 393–404.
Harvey, B. P., Gwynn-Jones, D. & Moore, P. J. (2013). Meta-analysis reveals complex marine biological responses to the interactive effects of ocean acidification and warming. Ecology and Evolution, 3, 1016–1030.
Hay, M. E. (1996). Defensive synergisms? Reply to Pennings. Ecology, 77, 1950–1952.
Hay, M. E., Kappel, Q. E. & Fenical, W. (1994). Synergisms in plant defenses against herbivores: interactions of chemistry, calcification, and plant quality. Ecology, 75, 1714–1726.
Hedges, L. V. & Olkin, I. (1985). Statistical Methods for Meta-analysis. New York, NY: Academic Press.
Heier, L. S., Teien, H. C., Oughton, D., et al. (2013). Sublethal effects in Atlantic salmon (Salmo salar) exposed to mixtures of copper, aluminium and gamma radiation. Journal of Environmental Radioactivity, 121, 33–42.
Heino, J., Schmera, D. & Erős, T. (2013). A macroecological perspective of trait patterns in stream communities. Freshwater Biology, 58, 1539–1555.
Hof, C., Araújo, M. B., Jetz, W. & Rahbek, C. (2011). Additive threats from pathogens, climate and land-use change for global amphibian diversity. Nature, 480, 516–519.
Hughes, R. M., Dunham, S., Maas-Hebner, K. G., et al. (2014). A review of urban water body challenges and approaches: (1) rehabilitation and remediation. Fisheries, 39, 18–29.
Hurlbert, S. H. (1984). Pseudoreplication and the design of ecological field experiments. Ecological Monographs, 54, 187–211.
Inendino, K. R., Grant, E. C., Philipp, D. P. & Goldberg, T. L. (2005). Effects of factors related to water quality and population density on the sensitivity of juvenile largemouth bass to mortality induced by viral infection. Journal of Aquatic Animal Health, 17, 304–314.
Jähnig, S., Lorenz, A. W., Hering, D., et al. (2011). River restoration success: a question of perception. Ecological Applications, 21, 2007–2015.
Johnson, J. E., Patterson, D. A., Martins, E. G., Cooke, S. J. & Hinch, S. G. (2012). Quantitative methods for analysing cumulative effects on fish migration success: a review. Journal of Fish Biology, 81, 600–631.
Jokinen, I. E., Salo, H. M., Markkula, E., et al. (2011). Additive effects of enhanced ambient ultraviolet B radiation and increased temperature on immune function, growth and physiological condition of juvenile (parr) Atlantic Salmon, Salmo salar. Fish & Shellfish Immunology, 30, 102–108.
Jones, D. T., Moffitt, C. M. & Peters, K. K. (2007). Temperature-mediated differences in bacterial kidney disease expression and survival in Renibacterium salmoninarum-challenged bull trout and other salmonids. North American Journal of Fisheries Management, 27, 695–706.
Kemp, P., Sear, D., Collins, A., Naden, P. & Jones, I. (2011). The impacts of fine sediment on riverine fish. Hydrological Processes, 25, 1800–1821.
Kocan, R., Hershberger, P., Sanders, G. & Winton, J. (2009). Effects of temperature on disease progression and swimming stamina in Ichthyophonus-infected rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 32, 835–843.
Lahnsteiner, F., Haunschmid, R. & Mansour, N. (2011). Possible reasons for late summer brown trout (Salmo trutta Linnaeus 1758) mortality in Austrian prealpine river systems. Journal of Applied Ichthyology, 27, 83–93.
Lange, K., Townsend, C. R., Gabrielsson, R., Chanut, P. C. M. & Matthaei, C. D. (2014). Responses of stream fish populations to farming intensity and water abstraction in an agricultural catchment. Freshwater Biology, 59, 286–299.
Lapointe, D., Pierron, F. & Couture, P. (2011). Individual and combined effects of heat stress and aqueous or dietary copper exposure in fathead minnows (Pimephales promelas). Aquatic Toxicology, 104, 80–85.
Lapointe, N. W. R., Cooke, S. J., Imhof, J. G., et al. (2014). Principles for ensuring healthy and productive freshwater ecosystems that support sustainable fisheries. Environmental Reviews, 22, 110–134.
Leino, R. L. & McCormick, J. H. (1993). Responses of juvenile largemouth bass to different pH and aliminium levels at overwintering temperatures – effects on gill morphology, electrolyte balance, scale calcium, liver glycogen, and depot fat. Canadian Journal of Zoology, 71, 531–543.
Levi, T., Darimont, C. T., MacDuffee, M., et al. (2012). Using grizzly bears to assess harvest-ecosystem tradeoffs in salmon fisheries. PLoS Biology, 10, e1001303.
Lindenmayer, D. B., Likens, G. E., Krebs, C. J. & Hobbs, R. J. (2010). Improved probability of detection of ecological ‘surprises’. Proceedings of the National Academy of Sciences, 107, 21957–21962.
Linton, T. K., Reid, S. D. & Wood, C. M. (1999). Effects of a restricted ration on the growth and energetics of juvenile rainbow trout exposed to a summer of simulated warming and sublethal ammonia. Transactions of the American Fisheries Society, 128, 758–763.
Luckenbach, T., Kilian, M., Triebskorn, R. & Oberemm, A. (2001). Fish early life stage tests as a tool to assess embryotoxic potentials in small streams. Journal of Aquatic Ecosystem Stress and Recovery, 8, 355–370.
Magaud, H., Migeon, B., Morfin, P., Garric, J. & Vindimian, E. (1997). Modelling fish mortality due to urban storm run-off: interacting effects of hypoxia and un-ionized ammonia. Water Research, 31, 211–218.
Mann, R. M., Hyne, R. V., Choung, C. B. & Wilson, S. P. (2009). Amphibians and agricultural chemicals: review of the risks in a complex environment. Environmental Pollution, 157, 2903–2927.
Matthaei, C. D., Piggott, J. J. & Townsend, C. R. (2010). Multiple stressors in agricultural streams: interactions among sediment addition, nutrient enrichment and water abstraction. Journal of Applied Ecology, 47, 639–649.
McCullough, D. A., Bartholow, J. M., Jager, H. I., et al. (2009). Research in thermal biology: burning questions for coldwater stream fishes. Reviews in Fisheries Science, 17, 90–115.
McKim, J. M. (1977). Evaluation of tests with early life stages of fish for predicting long-term toxicity. Journal of the Fisheries Research Board of Canada, 34, 1148–1154.
McLeod, E., Anthony, K. R. N., Andersson, A., et al. (2013). Preparing to manage coral reefs for ocean acidification: lessons from coral bleaching. Frontiers in Ecology and the Environment, 11, 20–27.
Minns, C. K. (2013). The science of ecosystem-based management on a global scale: the Laurentian Great Lakes, Lake Ontario, and the Bay of Quinte as a nested case study. Aquatic Ecosystem Health and Management, 16, 229–239.
Moran, R., Harvey, I., Moss, B., et al. (2010). Influence of simulated climate change and eutrophication on three-spined stickleback populations: a large scale mesocosm experiment. Freshwater Biology, 55(2), 315–325.
Morgan, I. J., D'Cruz, L. M., Dockray, J. J., et al. (1998). The effects of elevated winter temperature and sub-lethal pollutants (low pH, elevated ammonia) on protein turnover in the gill and liver of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 19, 377–389.
Morgan, I. J., McDonald, D. G. & Wood, C. M. (2001). The cost of living for freshwater fish in a warmer, more polluted world. Global Change Biology, 7, 345–355.
Nakagawa, S. & Cuthill, I. C. (2007). Effect size, confidence interval and statistical significance: a practical guide for biologists. Biological Reviews, 82, 591–605.
Olden, J. D., Poff, N. L. & Bestgen, K. R. (2008). Trait synergisms and the rarity, extirpation, and extinction risk of desert fishes. Ecology, 89, 847–856.
Olden, J. D., Kennard, M. J., Leprieur, F., et al. (2010). Conservation biogeography of freshwater fishes: recent progress and future challenges. Diversity and Distributions, 16, 496–513.
Ormerod, S., Dobson, M., Hildrew, A. & Townsend, C. (2010). Multiple stressors in freshwater ecosystems. Freshwater Biology, 55(S1), 1–4.
Paine, R. T., Tegner, M. J. & Johnson, E. A. (1998). Compounded perturbations yield ecological surprises. Ecosystems, 1, 535–545.
Palikova, M., Krejci, R., Hilscherova, K., et al. (2007). Effects of different oxygen saturation on activity of complex biomass and aqueous crude extract of cyanobacteria during embryonal development in carp (Cyprinus carpio L.). Acta Veterinaria Brno, 76, 291–299.
Palmer, M. A., Menninger, H. L. & Bernhardt, E. (2010). River restoration, habitat heterogeneity and biodiversity: a failure of theory or practice?Freshwater Biology, 55(S1), 205–222.
Park, R. A., Clough, J. S. & Wellman, M. C. (2008). AQUATOX: modeling environmental fate and ecological effects in aquatic ecosystems. Ecological Modelling, 213, 1–15.
Person-Le Ruyet, J., Labbe, L., Le Bayon, N., et al. (2008). Combined effects of water quality and stocking density on welfare and growth of rainbow trout (Oncorhynchus mykiss). Aquatic Living Resources, 21, 185–195.
Peuranen, S., Keinanen, M., Tigerstedt, C. & Vuorinen, P. J. (2003). Effects of temperature on the recovery of juvenile grayling (Thymallus thymallus) from exposure to Al+Fe. Aquatic Toxicology, 65, 73–84.
Piggott, J. J., Lange, K., Townsend, C. R. & Matthaei, C. D. (2012). Multiple stressors in agricultural streams: a mesocosm study of interactions among raised water temperature, sediment addition and nutrient enrichment. PLoS ONE, 7, e49873.
Pikitch, E. K., Santora, C., Babcock, E. A., et al. (2004). Ecosystem-based fishery management. Science, 305, 346–347.
Pilati, A., Vanni, M. J., Gonzalez, M. J. & Gaulke, A. K. (2009). Effects of agricultural subsidies of nutrients and detritus on fish and plankton of shallow-reservoir ecosystems. Ecological Applications, 19, 942–960.
Pinheiro, J., Bates, D., DebRoy, S. & Sarkar, D. (2014). nlme: Linear and Non-linear Mixed-effects Models. Vienna.
Poff, N. L., Allan, J. D, Bain, M. B., et al. (1997). The natural flow regime. Bioscience, 47, 769–784.
Potts, S. G., Biesmeijer, J. C., Kremen, C., et al. (2010). Global pollinator declines: trends, impacts and drivers. Trends in Ecology and Evolution, 25, 345–353.
Power, M. (1997). Assessing the effects of environmental stressors on fish populations. Aquatic Toxicology, 39, 151–169.
Priddis, E., Rader, R., Belk, M., Schaalje, B. & Merkley, S. (2009). Can separation along the temperature niche axis promote coexistence between native and invasive species?Diversity and Distributions, 15, 682–691.
Qiang, J., Xu, P., Wang, H., Li, R. & Wang, H. (2012). Combined effect of temperature, salinity and density on the growth and feed utilization of Nile tilapia juveniles (Oreochromis niloticus). Aquaculture Research, 43, 1344–1356.
Quinn, G. P. & Keough, M. J. (2002). Experimental Design and Data Analysis for Biologists. New York, NT: Cambridge University Press.
R Development Core Team. (2013). R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing.
Reese, C. D. & Harvey, B. C. (2002). Temperature-dependent interactions between juvenile steelhead and Sacramento pikeminnow in laboratory streams. Transactions of the American Fisheries Society, 131, 599–606.
Richardson, J., Williams, E. K. & Hickey, C. W. (2001). Avoidance behaviour of freshwater fish and shrimp exposed to ammonia and low dissolved oxygen separately and in combination. New Zealand Journal of Marine and Freshwater Research, 35, 625–633.
Roger, F., Godhe, A. & Gamfeldt, L. (2012). Genetic diversity and ecosystem functioning in the face of multiple stressors. PLoS ONE, 7, e45007.
Rutherford, J. C., Marsh, N. A., Davies, P. M. & Bunn, S. E. (2004). Effects of patchy shade on stream water temperature: how quickly do small streams heat and cool?Marine and Freshwater Research, 55, 737–748.
Salazar-Lugo, R., Estrella, A., Oliveros, A., et al. (2009). Paraquat and temperature affect nonspecific immune response of Colossoma macropomum. Environmental Toxicology and Pharmacology, 27, 321–326.
Salbu, B., Denbeigh, J., Smith, R. W., et al. (2008). Environmentally relevant mixed exposures to radiation and heavy metals induce measurable stress responses in Atlantic salmon. Environmental Science & Technology, 42, 3441–3446.
Serafy, J. E. & Harrell, R. M. (1993). Behavioral-response of fishes to increasing pH and dissolved-oxygen – field and laboratory observations. Freshwater Biology, 30, 53–61.
Shrimpton, J. M., Zydlewski, J. D. & Heath, J. W. (2007). Effect of daily oscillation in temperature and increased suspended sediment on growth and smolting in juvenile chinook salmon, Oncorhynchus tshawytscha. Aquaculture, 273, 269–276.
Skov, P. V., Larsen, B. K., Frisk, M. & Jokumsen, A. (2011). Effects of rearing density and water current on the respiratory physiology and haematology in rainbow trout, Oncorhynchus mykiss at high temperature. Aquaculture, 319, 446–452.
Sodhi, N. S., Bickford, D., Diesmos, A. C., et al. (2008). Measuring the meltdown: drivers of global amphibian extinction and decline. PLoS ONE, 3, e1636.
Stalnaker, C. B., Lamb, B. L., Henriksen, J., Boveee, K. & Bartholow, J. (1995). The Instream Flow Incremental Methodology: A Primer for IFIM. Washington, DC:USGS.
Stasko, A. D., Gunn, J. M. & Johnston, T. A. (2012). Role of ambient light in structuring north-temperate fish communities: potential effects of increasing dissolved organic carbon concentration with a changing climate. Environmental Reviews, 20, 173–190.
Statzner, B. & Bêche, L. A. (2010). Can biological invertebrate traits resolve effects of multiple stressors on running water ecosystems?Freshwater Biology, 55, 80–119.
Stoll, S., Sundermann, A., Lorenz, A. W., Kail, J. & Haase, P. (2013). Small and impoverished regional species pools constrain colonisation of restored river reaches by fishes. Freshwater Biology, 58, 664–674.
Suomalainen, L. R., Tiirola, M. A. & Valtonen, E. T. (2005). Influence of rearing conditions on Flavobacterium columnare infection of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 28, 271–277.
Swanson, S. M. (2004). Multiple Stressors: Literature Review and Gap Analysis (Water Environment Research Foundation Report 00-ECO-2B). London: International Water Association Publishing.
Townsend, C. R., Uhlmann, S. S. & Matthaei, C. D. (2008). Individual and combined responses of stream ecosystems to multiple stressors. Journal of Applied Ecology, 45, 1810–1819.
Valenti, T. W., Perez-Hurtado, P., Chambliss, C. K. & Brooks, B. W. (2009). Aquatic toxicity of sertraline to Pimephales promelas at environmentally relevant surface water pH. Environmental Toxicology and Chemistry, 28, 2685–2694.
Valenti, T. W., James, S. V., Lahousse, M. J., et al. (2010). A mechanistic explanation for pH-dependent ambient aquatic toxicity of Prymnesium parvum carter. Toxicon, 55, 990–998.
Van der Oost, R., Beyer, J. & Vermeulen, N. P. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13, 57–149.
Vanhoudt, N., Vandenhove, H., Real, A., Bradshaw, C. & Stark, K. (2012). A review of multiple stressor studies that include ionising radiation. Environmental Pollution, 168, 177–192.
Vera Cartas, J., Pucheu, K. & Torres Beristain, B. (2013). Contributions towards an ecosystem based management of Lake Titicaca. Aquatic Ecosystem Health & Management, 16, 240–247.
Verberk, W. C. E. P., van Noordwijk, C. G. E. & Hildrew, A. G. (2013). Delivering on a promise: integrating species traits to transform descriptive community ecology into a predictive science. Freshwater Science, 32, 531–547.
Vinebrooke, R. D., Cottingham, K. L., Norberg, J., et al. (2004). Impacts of multiple stressors on biodiversity and ecosystem functioning: the role of species co-tolerance. Oikos, 104, 451–457.
Von Westernhagen, H. (1988). Four sublethal effects of pollutants on fish eggs and larvae. In Fish Physiology. London: Academic Press, pp. 253–346.
Vörösmarty, C. J., McIntyre, P. B., Gessner, M. O., et al. (2010). Global threats to human water security and river biodiversity. Nature, 467, 555–561.
Wagenhoff, A., Lange, K., Townsend, C. R. & Matthaei, C. D. (2013). Patterns of benthic algae and cyanobacteria along twin-stressor gradients of nutrients and fine sediment: a stream mesocosm experiment. Freshwater Biology, 58, 1849–1863.
Wagenhoff, A., Townsend, C. R. & Matthaei, C. D. (2012). Macroinvertebrate responses along broad stressor gradients of deposited fine sediment and dissolved nutrients: a stream mesocosm experiment. Journal of Applied Ecology, 49, 892–902.
Wagner, E. J., Bosakowski, T. & Intelmann, S. (1997). Combined effects of temperature and high pH on mortality and the stress response of rainbow trout after stocking. Transactions of the American Fisheries Society, 126, 985–998.
Walsh, C. J., Fletcher, T. D. & Ladson, A. R. (2005). The urban stream syndrome: current knowledge and the search for a cure. Journal of the North American Benthological Society, 24, 706–723.
Waters, T. F. (1995). Sediment in Streams: Sources, Biological Effects, and Control.Bethesda, MD: American Fisheries Society.
Wenger, S. J., Roy, A. H., Jackson, C. R., et al. (2009). Twenty-six key research questions in urban stream ecology: an assessment of the state of the science. Journal of the North American Benthological Society, 28, 1080–1098.
Wood, P. J. & Armitage, P. D. (1997). Biological effects of fine sediment in the lotic environment. Environmental Management, 21, 203–217.
Woodward, G., Perkins, D. M. & Brown, L. E. (2010). Climate change and freshwater ecosystems: impacts across multiple levels of organization. Philosophical Transactions of the Royal Society B: Biological Sciences, 365, 2093–2106.
Yen, J. D. L., Bond, N. R., Shenton, W., Spring, D. A. & Mac Nally, R. (2013). Identifying effective water-management strategies in variable climates using population dynamics models. Journal of Applied Ecology, 50, 691–701.