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Chapter 8 - The Clinical In-Vitro Fertilization Laboratory

Published online by Cambridge University Press:  24 December 2019

Kay Elder
Affiliation:
Bourn Hall Clinic, Cambridge
Brian Dale
Affiliation:
Centre for Assisted Reproduction, Naples
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Summary

In the armory of medical technology available for alleviation of disease and quality of life enhancement, there is nothing to match the unique contribution of assisted reproductive technology (ART). There is no other life experience that matches the birth of a baby in significance and importance. The responsibility of nurturing and watching children grow and develop alters the appreciation of life and health, with a resulting long-term impact upon individuals, families and, ultimately, society. Thus, the combination of oocyte and sperm to create an embryo with the potential to develop into a unique individual cannot be regarded lightly, as merely another form of invasive medical technology, but must be treated with the respect and responsibility merited by the most fundamental areas of human life.

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Chapter
Information
In-Vitro Fertilization , pp. 156 - 179
Publisher: Cambridge University Press
Print publication year: 2020

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References

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Summers, MC, Biggers, JD (2003) Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Human Reproduction Update 9(6): 557582.CrossRefGoogle ScholarPubMed
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Ashwood-Smith, MJ, Hollands, P, Edwards, RG (1989) The use of Albuminar (TM) as a medium supplement in clinical IVF. Human Reproduction 4: 702705.CrossRefGoogle Scholar
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Cutting, R, Pritchard, J, Clarke, H, Martin, K (2004) Establishing quality control in the new IVF laboratory. Human Fertility 7(2): 119125.CrossRefGoogle ScholarPubMed
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Dumoulin, JC, Land, JA, Van Montfoort, AP, et al. (2010) Effect of in vitro culture of human embryos on birthweight of newborns. Human Reproduction 25(3): 605612.CrossRefGoogle ScholarPubMed
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Elder, K, Van den Bergh, M, Woodward, B (2015) The IVF culture system. In: Elder, K, Van den Bergh, M, Woodward, B (2015) Troubleshooting and Problem-Solving in the IVF Laboratory. Cambridge University Press, Cambridge, UK., pp. 2843.CrossRefGoogle Scholar
Erbach, GT, Bhatnagar, P, Baltz, JM, Biggers, JD (1995) Zinc is a possible toxic contaminant of silicone oil in microdrop cultures of preimplantation mouse embryos. Human Reproduction 10: 32483254.CrossRefGoogle ScholarPubMed
Fleetham, J, Mahadevan, MM (1988) Purification of water for in vitro fertilization and embryo transfer. Journal of In Vitro Fertilization and Embryo Transfer 5: 171174.CrossRefGoogle ScholarPubMed
Fleming, TP, Pratt, HPM, Braude, PR (1987) The use of mouse preimplantation embryos for quality control of culture reagents in human in vitro fertilisation programs: a cautionary note. Fertility and Sterility 47: 858860.CrossRefGoogle ScholarPubMed
Gardner, D (1999) Development of new serum-free media for the culture and transfer of human blastocysts. Human Reproduction 13(Suppl. 4): 218225.CrossRefGoogle Scholar
George, MA, Braude, PR, Johnson, MH, Sweetnam, DG (1989) Quality control in the IVF laboratory: in vitro and in vivo development of mouse embryos is unaffected by the quality of water used in culture media. Human Reproduction 4: 826831.CrossRefGoogle ScholarPubMed
Guo, KJ (2015) “By the work, one knows the workman”: the practice and profession of the embryologist and its translation to quality in the embryology laboratory. Reproductive BioMedicine Online 31: 449458.CrossRefGoogle Scholar
Harvey, AJ, Rathjen, J, Yu, LJ, Gardner, DK (2016) Oxygen modulates human embryonic stem cell metabolism in the absence of changes in self-renewal. Reproduction Fertility and Development 28(4): 446458.CrossRefGoogle ScholarPubMed
Heitman, RJ, Hill, MJ, James, AN, Schimmel, T, et al. (2015) Live births achieved via IVF are increased by improvements in air quality and laboratory environment. Reproductive BioMedicine Online 31: 364371.CrossRefGoogle Scholar
Heo, YS, Cabrera, LM, Bormann, CL, et al. (2010) Dynamic microfunnel culture enhances mouse embryo development and pregnancy rates. Human Reproduction 25(3): 613622.CrossRefGoogle ScholarPubMed
Johnson, C, Hofmann, G, Scott, R (1994) The use of oil overlay for in vitro fertilisation and culture. Assisted Reproduction Review 4: 198201.Google Scholar
Kane, MT, Morgan, PM, Coonan, C (1997) Peptide growth factors and preimplantation development. Human Reproduction Update 3(2): 137157.CrossRefGoogle ScholarPubMed
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