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Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells

Published online by Cambridge University Press:  16 October 2014

Bartosz Kempisty ,
Agnieszka Ziółkowska ,
Sylwia Ciesiółka ,
Hanna Piotrowska ,
Paweł Antosik ,
Dorota Bukowska ,
Klaus P. Brüssow ,
Michał Nowicki  and
Maciej Zabel
Bartosz Kempisty*
Affiliation:
Department of Histology and Embryology, Department of Anatomy, PoznanUniversity of Medical Sciences, 6 Swiecickiego St., 60–781 Poznan, Poland. Department of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St. 60–781 Poznan, Poland.
Agnieszka Ziółkowska
Affiliation:
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St. 60–781 Poznan, Poland.
Sylwia Ciesiółka
Affiliation:
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St. 60–781 Poznan, Poland.
Hanna Piotrowska
Affiliation:
Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St. 60–631 Poznan, Poland.
Paweł Antosik
Affiliation:
Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego St. 60–628, Poznan, Poland.
Dorota Bukowska
Affiliation:
Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego St. 60–628, Poznan, Poland.
Klaus P. Brüssow
Affiliation:
Institute of Reproductive Biology, Department of Experimental Reproductive Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.
Michał Nowicki
Affiliation:
Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St. 60–781 Poznan, Poland.
Maciej Zabel
Affiliation:
Department of Histology and Embryology, Wroclaw Medical University, 6a Chalubinskiego St., 50–368 Wroclaw, Poland.
*
All correspondence to: Bartosz Kempisty. Department of Histology and Embryology, Department of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60–781 Poznan, Poland. Tel: +48 61 8546419. Fax: +48 61 8546455. E-mail: etok@op.pl
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Summary

Although the expression of estrogen and progesterone receptors within porcine ovary and cumulus–oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to real-time proliferation. In this study, a model of oocytes-separated cumulus cells was used to analyze the cell proliferation index and the expression PGR, PGRMC1 and of ERRγ and ERRβ/γ during 96-h cultivation in vitro using real-time quantitative PCR (qRT-PCR) and confocal microscopic observation. We found that PGR protein expression was increased at 0 h, compared with PGR protein expression after 96 h of culture (P < 0.001). The expression of PGRMC1, ERRγ and ERRβ/γ was unchanged. After using qRT-PCR we did not found statistical differences in expression of PGR, PGRMC1, ERRγ and ERRβ/γ during 96 h of cumulus cells in vitro culture (IVC). We supposed that the differential expression of the PGR protein at 0 h and after 96 h is related to a time-dependent down-regulation, which may activate a negative feedback. The distribution of PGR, PGRMC1 proteins may be linked with the translocation of receptors to the cytoplasm after the membrane binding of respective agonists and intra-cytoplasmic signal transduction. Furthermore, cumulus cells analyzed at 0 h were characterized by decreased proliferation index, whereas those after 96 h of culture revealed a significant increase of proliferation index, which may be associated with differentiation/luteinization of these cells during real-time proliferation.

Keywords

Cumulus cellsEstrogen receptorsPorcine oocyte–cumulus complexesProgesterone receptorsProliferation index
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 836 - 845
Copyright
Copyright © Cambridge University Press 2014 

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