Characterizing ruminal parameters in the context of sampling routine and feed efficiency is fundamental to understand the efficiency of feed utilization in the bovine. Therefore, we evaluated microbial and volatile fatty acid (VFA) profiles, rumen papillae epithelial and stratum corneum thickness and rumen pH (RpH) and temperature (RT) in feedlot cattle. In all, 48 cattle (32 steers plus 16 bulls), fed a high moisture corn and haylage-based ration, underwent a productive performance test to determine residual feed intake (RFI) using feed intake, growth, BW and composition traits. Rumen fluid was collected, then RpH and RT logger were inserted 5.5±1 days before slaughter. At slaughter, the logger was recovered and rumen fluid and rumen tissue were sampled. The relative daily time spent in specific RpH and RT ranges were determined. Polynomial regression analysis was used to characterize RpH and RT circadian patterns. Animals were divided into efficient and inefficient groups based on RFI to compare productive performance and ruminal parameters. Efficient animals consumed 1.8 kg/day less dry matter than inefficient cattle (P⩽0.05) while achieving the same productive performance (P⩾0.10). Ruminal bacteria population was higher (P⩽0.05) (7.6×1011v. 4.3×1011 copy number of 16S rRNA gene/ml rumen fluid) and methanogen population was lower (P⩽0.05) (2.3×109v. 4.9×109 copy number of 16S rRNA gene/ml rumen fluid) in efficient compared with inefficient cattle at slaughter with no differences (P⩾0.10) between samples collected on-farm. No differences (P⩾0.10) in rumen fluid VFA were also observed between feed efficiency groups either on-farm or at slaughter. However, increased (P⩽0.05) acetate, and decreased (P⩽0.05) propionate, butyrate, valerate and caproate concentrations were observed at slaughter compared with on-farm. Efficient had increased (P⩽0.05) rumen epithelium thickness (136 v. 126 µm) compared with inefficient cattle. Efficient animals also spent 318% and 93.2% more time (P⩽0.05) in acidotic (4.14% v. 1.30%) (pH⩽5.6) and optimal (5.6<pH<6.0) (8.53% v. 4.42%) RpH range compared with inefficient cattle. The circadian patterns revealed lower (P⩽0.05) RpH and no differences (P⩾0.10) in RT pre-, during, and post-prandial periods in efficient compared with inefficient cattle. In essence, superior feed efficiency in cattle seems linked to rumen features consistent with improved efficiency of feed utilization. Microbial abundance, rumen epithelial histomorphology, and RpH, may serve as indicators for feed efficiency in cattle. The divergences of assessments made on-farm and at slaughter should be considered in the development of proxies for feed efficiency.

Dunes adjacent to the Snow Water Lake playa in Elko County of northeastern Nevada rise up to ~10 m above the playa surface in seven distinct clusters. The dunes are composed of tan silty loam containing calcite, quartz, plagioclase, and dioctahedral clay. Abundances of trace elements, along with relative proportions of quartz and calcite, are distinct between dunes along the north and south sides of the playa, reflecting proximity to streams draining different lithologies in the neighboring mountains. Luminescence (optically stimulated luminescence and infrared-stimulated luminescence) dating of dune crest samples demonstrates that the last episode of dune accumulation occurred in the mid-eighteenth century. Moisture-sensitive tree ring records from a nearby site indicate that dune accumulation coincided with an interval of below-average precipitation immediately following a very wet decade. This sequence is consistent with models requiring wetter climatic conditions to move coarse sediment onto a playa surface, followed by dune building under drier conditions. Younger luminescence ages from a sand-dominated unit exposed in an arroyo cut through the dunes may reflect a wetter, more erosive climatic regime ca. AD 1800. The Snow Water Lake dunes are currently eroding, signaling a reduction in the amount of sediment reaching the playa.

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Attempts were made to identify the causative organism of Lyme disease in Australia from possible tick vectors.

Ticks were collected in coastal areas of New South Wales, Australia, from localities associated with putative human infections. The ticks were dissected; a portion of the gut contents was examined for spirochaetes by microscopy, the remaining portion inoculated into culture media. The detection of spirochaetes in culture was performed using microscopy, and immunochemical and molecular (PCR) techniques. Additionally, whole ticks were tested with PCR for spirochaetes.

From 1990 to 1992, approximately 12000 ticks were processed for spirochaetes. No evidence of Borrelia burgdorferi or any other spirochaete was recovered from or detected in likely tick vectors. Some spirochaete–like objects detected in the cultures were shown to be artifacts, probably aggregates of bacterial flagellae.

There is no definitive evidence for the existence in Australia of B. burgdorferi the causative agent of true Lyme disease, or for any other tick–borne spirochaete that may be responsible for a local syndrome being reported as Lyme disease.

The degree of exposure of a beach to wave action largely determines particle size, sorting and beach gradient (King, 1959) and these, in turn, have a dominant effect on the animal communities within the sand (Swedmark, 1964; Mclntyre, 1969). It is likely, however, that the main turnover of energy, as indicated by the rapid removal of oxygen (Brafield, 1964; Hamilton & Greenfield, 1967), is due to bacteria and algae. On sheltered muddy beaches, motile diatoms can be of considerable importance (Aleem, 1950; Perkins, 1960) but for a relatively exposed beach at Firemore, Loch Ewe, on the west coast of Scotland, Fig. 1, there was negligible unattached plant material, living or dead, and the primary production by the diatoms attached to the sand grains was low (Steele & Baird, 1968). Further, these diatoms did not show evidence of heterotrophy (Munro & Brock, 1968; Mclntyre, Munro & Steele, 1970) yet, at low water mark on this beach, the populations of attached diatoms are relatively uniform to a depth of 20–30 cm in the sand and show no sign of pigment degradation or decreased photosynthetic ability. These conditions at low water mark differ from those at a water depth of 5–10 m where chlorophyll concentration in the upper 5 cm of the sand is higher but where pigment degradation and blackening of the sand, indicating reducing conditions, are usually found below 10 cm depth of sand.

Phosphorus requirements for maintenance and growth were assessed by giving to growing lambs and non-pregnant ewes diets low in P and to growing lambs a diet containing the quantity of P recommended by the Agricultural Research Council (1980).

Seven 14-week-old lambs were given ad libitum a pelleted diet containing 1·88 (LP) or 2·75 (NP) g P/kg D. M. for 13 weeks. There was no effect of P intake on feed intake or growth during the first 8 weeks when the P intake of the LP groups was 1·07 of Agricultural Research Council (1980) requirements. During the last 5 weeks the P intake of the LP group was only 0·87 of Agricultural Research Council (1980) requirements and growth and feed intake were higher in the male but not female lambs in the NP group. From the 3rd week the plasma concentration of P was significantly lower (P <0·001) in the LP group. Mineralization of selected bones was less in the LP group.

After 14 weeks on the diet, balance trials with 32 P were carried out on the lambs from each dietary group. P intakes ranged from 1 to 3 g/day. An additional balance trial was undertaken with eight 2-year-old female sheep consuming a hay diet (1·5 g P/day).

The endogenous faecal excretion of P by the growing lambs on the LP diet and ewes was 12·2 ± 0·8 and 12·7 ± 1·00 mg/kg live weight/day respectively, very close to the value (12 mg/kg live weight/day) adopted by the Agricultural Research Council (1980). The absorbability of the P in the concentrate and hay diets was 0.71 ±0·267 and 0·74 ± 0·0218 respectively, the former agreeing with and the latter higher than the corresponding value for Agricultural Research Council (1980).

The literature describes increasing acuity on inpatient psychiatric wards. This pressure is sometimes implicated in reduced effectiveness of service delivery. Policy changes have resulted in the gradual reduction in the number of inpatient beds. This has been supported by an increase in community mental health service provision with the community now being the preferred setting. This paper reviews current literature to debate the concept of acuity and its usefulness for mental health care in the UK. The findings suggest the concept of acuity can be misleading for services. The effectiveness of inpatient care is dependent on numerous factors, of which acuity is only one component. To focus on acuity as a measure of inpatient care detracts energy and resources from those factors which can impact on and improve inpatient mental health care and serves only to blame service users for being too ill for the service to cope with.

Furunculosis has caused serious economic losses in some Atlantic salmon farms in Scotland. It is considered that the disease has the potential to be a significant economic problem to the whole industry unless control measures are adopted. When salmon in a farmed population carry the disease agent in non-clinical form the bacterium has low to intermediate virulence, but in stocks with recurring clinical disease, virulence is apparently increased. The significance of these observations is discussed in terms of the major methods of control, namely avoidance, husbandry procedures, chemotherapy, vaccines and statutory controls.

Investigation of a temperate and tropical beach has shown that, based on chlorophyll a analyses, an epipsammic diatom population present in the temperate beach is absent in the tropical beach. Comparison of community respiration rates for the two beach populations excluding macrofaunal respiration shows that the tropical beach rates (which were constant over the period January-March) were about 9 times the winter rates of the temperate beach and about twice the summer rates. Annual microbial production is estimated at 15 gC/m2 for the temperate beach and 72 gC/m2 for the tropical beach. The greatly increased rate of production in the tropical beach was considered to be a function of increased water flow into that beach. In comparison, meiofauna and macrofauna biomass are less in the tropical beach and their respective production likely to be at most no more than in the temperate beach. It is proposed from experimental studies that the failure of animal populations fully to utilise microbial production in sandy beaches is due to the relative degree of exposure stripping significant quantities of microbial growth from this system. This proposal is discussed as a means of explaining mechanisms of control of animal production in sandy beaches.

A 3-year survey of wild fish in Loch Awe and the surrounding watershed has shown a restricted distribution and low prevalence of infection with infectious pancreatic necrosis virus. No signs of clinical disease were observed in infected fish. The restricted distribution close to a rainbow trout farm is consistent with the virus originating from this farm, where virus infected stocks have been found each year since 1971. The continuing low prevalence is also consistent with virus in wild fish resulting from the spread of infection from the farm. If infection is spreading between wild fish, it is slow within the time scale of the survey. The possible mechanisms of transmission of infection from the farm to wild fish are discussed.