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The transcription map of the human genome published by Schuler
et al. (1996) is a valuable
resource in which approximately one quarter of all human genes have been
mapped with respect to
genetic framework markers using radiation hybrids. We have taken information
from this map to
provide potential genes within the TSC1 candidate region on chromosome
In so doing we have
been able to provide an independent assay of the quality of the radiation
hybrid mapping by using
somatic cell hybrids and a 2 Mb cosmid contig covering the TSC1 region
mapping tools. In
addition, we have built sequence contigs of ESTs for 25 clusters. This
shown that about 20% of
the relevant EST clusters in the Unigene resource (Boguski &
Schuler 1995) contain chimaeric clones.
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