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Sperm cryopreservation plays an important role in the field of male infertility and reproductive medicine. A donor sperm cryopreservation program has been developed and improved in mainland China. The conventional approach to sperm cryopreservation is to simply dilute semen with cryoprotectant and cryopreserve in liquid nitrogen until the sperm samples are thawed for use. Patients with spinal cord injuries often have a problem with poor sperm production as well as ejaculation after the damage. Electroejaculation is usually performed under a general anesthesia while the patient is placed in lateral decubitus. With the advance of new approaches for sperm vitrification, treatment of male infertility will become more effective without using sperm donors. Using vitrification for cryopreservation of sperm obtained from testicular biopsy, epididymal fluid, or a semen sample after electroejaculation could create new hope for infertile men.