Data are presented which amplify previously published work on the additive antiseptic effect of Roccal as follows:
1. Smallpox calf pulp derived from animals whose skin is treated with Roccal during the viral incubation period apparently adsorbs sufficient concentration of the reagent to produce a measurable bactericidal effect.
2. The adsorbed antiseptic induces a significant additive effect well beyond that of the action of stated concentrations of phenol and ether alone, as evidenced by the fact that this combination produces rapid diminution of the bacterial flora in highly contaminated calf lymph.
3. Vaccines containing the combination of antiseptics described caused no dermatitis or other untoward reactions in rabbits and humans and their viral potency was not affected.
4. The use of the antiseptic reagents makes possible the rapid preparation of smallpox vaccines for human use. Acceptable bacterial flora levels are obtained usually within 24 hr. of preparation.
The author is indebted to Magda Gagliardi, Marie Mazzochi and Izola Prunty for their assistance in the performance of the many bacterial counts, toxicity tests and other technical work.