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Serum cholesterol levels preceding to suicide death in Japanese workers: a nested case-control study

Published online by Cambridge University Press:  01 July 2019

Sanmei Chen*
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan
Tetsuya Mizoue
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan
Huanhuan Hu
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan
Keisuke Kuwahara
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan Teikyo University Graduate School of Public Health, Tokyo, Japan
Toru Honda
Affiliation:
Hitachi, Ltd., Ibaraki, Japan
Shuichiro Yamamoto
Affiliation:
Hitachi, Ltd., Ibaraki, Japan
Tohru Nakagawa
Affiliation:
Hitachi, Ltd., Ibaraki, Japan
Toshiaki Miyamoto
Affiliation:
Nippon Steel & Sumitomo Metal Corporation Kimitsu Works, Chiba, Japan
Hiroko Okazaki
Affiliation:
Mitsui Chemicals, Inc., Tokyo, Japan
Makiko Shimizu
Affiliation:
Mizue Medical Clinic, Keihin Occupational Health Center, Kanagawa, Japan
Taizo Murakami
Affiliation:
Mizue Medical Clinic, Keihin Occupational Health Center, Kanagawa, Japan
Masafumi Eguchi
Affiliation:
Furukawa Electric Co., Ltd., Tokyo, Japan
Takeshi Kochi
Affiliation:
Furukawa Electric Co., Ltd., Tokyo, Japan
Makoto Yamamoto
Affiliation:
Yamaha Corporation, Shizuoka, Japan
Takayuki Ogasawara
Affiliation:
Mitsubishi Fuso Truck and Bus Corporation, Kanagawa, Japan
Naoko Sasaki
Affiliation:
Mitsubishi Fuso Truck and Bus Corporation, Kanagawa, Japan
Akihiko Uehara
Affiliation:
Seijinkai Shizunai Hospital, Hokkaidō, Japan
Teppei Imai
Affiliation:
Azbil Corporation, Tokyo, Japan
Akiko Nishihara
Affiliation:
Department of Global Public Health, University of Tsukuba, Ibaraki, Japan
Ai Hori
Affiliation:
All Japan Labour Welfare Foundation, Tokyo, Japan
Satsue Nagahama
Affiliation:
Mitsubishi Plastics, Inc., Tokyo, Japan
Kentaro Tomita
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan
Maki Konishi
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan
Isamu Kabe
Affiliation:
Furukawa Electric Co., Ltd., Tokyo, Japan
Seitaro Dohi
Affiliation:
Mitsui Chemicals, Inc., Tokyo, Japan
for the Japan Epidemiology Collaboration on Occupational Health Study Group
Affiliation:
Department of Epidemiology and Prevention, National Center for Global Health and Medicine, Tokyo, Japan Teikyo University Graduate School of Public Health, Tokyo, Japan Hitachi, Ltd., Ibaraki, Japan Nippon Steel & Sumitomo Metal Corporation Kimitsu Works, Chiba, Japan Mitsui Chemicals, Inc., Tokyo, Japan Mizue Medical Clinic, Keihin Occupational Health Center, Kanagawa, Japan Furukawa Electric Co., Ltd., Tokyo, Japan Yamaha Corporation, Shizuoka, Japan Mitsubishi Fuso Truck and Bus Corporation, Kanagawa, Japan Seijinkai Shizunai Hospital, Hokkaidō, Japan Azbil Corporation, Tokyo, Japan Department of Global Public Health, University of Tsukuba, Ibaraki, Japan All Japan Labour Welfare Foundation, Tokyo, Japan Mitsubishi Plastics, Inc., Tokyo, Japan
*
Author for correspondence: Sanmei Chen, Email: schen@hosp.ncgm.go.jp
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Abstract

Objective:

To investigate the association between suicide death and serum cholesterol levels as measured at times close to suicide death.

Methods:

We conducted a nested case-control study of 41 cases of suicide deaths and 205 matched controls with serum total cholesterol (TC) levels till 3 years before suicide death in a large cohort of Japanese workers.

Results:

Individuals in the lowest versus highest tertile/predefined category of TC in a Japanese working population had a three- to four-fold greater risk of suicide death. Each 10 mg/dl decrement of average TC was associated with an 18% increased chance of suicide death (95% confidence interval, 2–35%). Similar results were found for TC levels at each year.

Conclusion:

These results suggest that a low serum TC level in recent past is associated with an increased risk of suicide death.

Type
Short Communication
Copyright
© Scandinavian College of Neuropsychopharmacology 2019 

Significant outcomes

  • A lower averaged level of TC across 3 years before suicide death was significantly associated with an increased risk of suicide death.

  • This association remained statistically significant when the averaged TC level was treated as a continuous or a categorical variable.

  • Similar results were found for TC levels at each single year.

Limitations

  • A relatively small number of cases.

  • The possibility of bias due to residual confounding effects and unmeasured confounders.

  • The limited generalisability of the results to populations with different backgrounds.

Introduction

Suicide is among the leading causes of death worldwide. In Japan, which has one of the highest global suicide rates, suicide is the top cause of death among young adults (Ministry of Health Labour and Welfare of Japan, 2018). Suicide, in addition to several psychiatric disorders (De Berardis et al., Reference De Berardis, Marini, Piersanti, Cavuto, Perna, Valchera, Mazza, Fornaro, Iasevoli, Martinotti and Di Giannantonio2012, Reference De Berardis, Serroni, Campanella, Marini, Rapini, Valchera, Iasevoli, Mazza, Fornaro, Perna, Di Iorio, Martinotti and Di Giannantonio2017, Reference De Berardis, Serroni, Marini, Rapini, Carano, Valchera, Iasevoli, Mazza, Signorelli, Aguglia, Perna, Martinotti, Varasano, Pressanti and Di Giannantonio2014), has been linked to nutritional biomarkers including serum cholesterol (Mittendorfer & Wasserman, Reference Mittendorfer and Wasserman2000). Low serum total cholesterol (TC) level has been found to be related to lower central nervous system serotonin activity (Steegmans et al., Reference Steegmans, Fekkes, Hoes, Bak, van der Does and Grobbee1996), a condition often observed in cases of depression and suicide. However, epidemiological evidence regarding the association between serum TC concentrations and the risk of suicide death is conflicting, with some studies showing an inverse association (Lindberg et al., Reference Lindberg, Råstam, Gullberg and Eklund1992; Neaton et al., Reference Neaton, Blackburn, Jacobs, Kuller, Lee, Sherwin, Shih, Stamler and Wentworth1992; Zureik et al., Reference Zureik, Courbon and Ducimetière1996; Partonen et al., Reference Partonen, Haukka, Virtamo, Taylor and Lonnqvist1999; Ellison & Morrison, Reference Ellison and Morrison2001; Jee et al., Reference Jee, Kivimaki, Kang, Park, Samet and Batty2011) and others suggesting a null (Kromhout et al., Reference Kromhout, Katan, Menotti, Keys and Bloemberg1992; Smith et al., Reference Smith, Shipley, Marmot and Rose1992; Giltay et al., Reference Giltay, Zitman, Menotti, Nissinen, Jacobs, Adachi, Kafatos and Kromhout2010; Chang et al., Reference Chang, Wen, Tsai, Lawlor, Yang and Gunnell2012) or even positive (Iribarren et al., Reference Iribarren, Reed, Wergowske, Burchfiel and Dwyer1995; Tanskanen et al., Reference Tanskanen, Vartiainen, Tuomilehto, Viinamäki, Lehtonen and Puska2000; Svensson et al., Reference Svensson, Inoue, Sawada, Charvat, Mimura and Tsugane2017) association. Furthermore, no data are available regarding circulating TC levels during the years immediately preceding suicide death. In this study, we examined serum TC levels as measured during the 3 years before suicide death among a large cohort of Japanese workers. We hypothesised that low levels of serum TC are associated with an increased risk of suicide death.

Materials and methods

Study design

We performed a case-control study nested in a cohort of the Japan Epidemiology Collaboration on Occupational Health (J-ECOH) Study, which is an ongoing study among workers at multiple companies (Hu et al., Reference Hu, Mizoue, Sasaki, Ogasawara, Tomita, Nagahama, Hori, Nishihara, Imai, Yamamoto, Eguchi, Kochi, Miyamoto, Honda, Nakagawa, Yamamoto, Okazaki, Uehara, Shimizu, Murakami, Kuwahara, Nanri, Konishi, Kabe and Dohi2018). A total of 146 619 participants who attended health check-ups at least once between January 2008 and December 2016 or between April 2008 and March 2017 comprised the sample for the present study. The research protocol was approved by the Ethics Committee of the National Center for Global Health and Medicine in Japan.

Measures

Ascertainment of suicide death and control selection

Using within-study registration, we ascertained participants’ deaths and their causes of death since April 2012. The dataset was locked for the present study on 10 August 2018. Causes of deaths were confirmed based on death certificates, sick leave documents, family confirmation and other sources. The causes of deaths were classified according to the International Statistical Classification of Diseases and Related Health Problems, 10th revision (ICD-10). Of the 60 suicide deaths (ICD-10 codes X60–X84) we ascertained, 19 cases were excluded owing to a lack of TC measurements conducted at any health check-up during the 3 years prior to suicide death, leaving 41 cases for analysis. Controls were selected by using the incidence density method. For each case of suicide death, we first created a pool of controls matched on sex, birthday (±2 years) and worksite. We then allocated the date of suicide death as an index date to matched controls. For a given case, we randomly selected five controls from the pool of eligible controls. We did not allow cases to be chosen as controls, nor did we allow a selected control to serve as a control of other cases.

Serum total cholesterol and other variables

Serum TC levels were analysed enzymatically. Analyses of TC were performed by laboratories that hold a high rank (Rank A or Score > 95/100 according to external quality control agencies). Of 41 cases, 28 had three recorded TC measurements, 5 had two TC records, and 8 had only one TC measurement. We calculated a mean TC level using the available data. Covariates included smoking status, lipid-lowering treatment, diabetes and hypertension at 1 year prior to suicide death. If these data were not available, measurements obtained 2 or 3 years prior to death were used.

Statistical analyses

We built conditional logistic regression models to estimate odds ratios (OR) and 95% confidence intervals (CI) of suicide death according to the 3-year average of TC levels. We used continuous (each 10 mg/dl decrement), tertile and predefined categories of averaged TC (<180 mg/dl, 180 to <200 mg/dl, 200 to <220 mg/dl and ≥220 mg/dl) as independent variables in separate models. We also adjusted for current smoking status, lipid-lowering treatment, diabetes and hypertension. We assessed trend association by assigning ordinal numbers to TC categories and modelling this as a continuous variable. We repeated the analyses using TC levels at single time points. We tested the interaction of smoking status on the potential association between TC and the risk of suicide death. We performed two sensitivity analyses: (1) excluding subjects who were undergoing lipid-lowering treatment and (2) using data comprising cases that had two or more TC measurements and their matched controls. Statistical analyses were performed using SAS version 9.4.

Results

There were no significant differences in the mean of body mass index, glycated hemoglobin, systolic blood pressure and diastolic blood pressure, nor in the rate of current smoker, lipid-lowering treatment, diabetes or hypertension between cases and controls (Table 1). Cases had significantly higher blood glucose levels than did controls.

Table 1. Characteristics of suicide cases and controls at 1 year prior to suicide/index date

SD, standard deviation; BMI, body mass index; HbA1c, glycated hemoglobin.

* Comparisons between cases and controls using chi-square test for categorical variables, t-test for continuous variables.

The risk of suicide death increased with decreasing serum TC levels across the 3 years preceding suicide death (Table 2). Compared with the highest tertile (≥217 mg/dl), the adjusted OR (95% CI) was 1.49 (0.53, 4.16) for the middle tertile and 4.68 (1.67, 13.14) for the lowest tertile (<188 mg/dl) (p for trend = 0.003). In analyses using the predefined categories with TC levels ≥ 220 mg/dl as a reference, the adjusted OR (95%CI) was 1.72 (0.53, 5.55) for TC levels 200 to <220 mg/dl, 2.80 (0.96, 8.14) for TC levels 180 to <200 mg/dl and 3.29 (1.10, 9.85) for TC levels <180 mg/dl (p for trend = 0.02). Each 10 mg/dl decrement of average TC was associated with an 18% increased chance of suicide death (95% CI, 2–35%). The interaction between smoking status and average TC was not statistically significant (p for interaction = 0.17). The results were similar when examining the associations using TC levels recorded during each year before suicide/index date (Supplementary Table 1).

Table 2. Associations between 3-year average of serum total cholesterol (TC) levels and the risk of suicide death

OR, odds ratio; CI, confidence interval.

* Adjusted for smoking (current smoker or not), lipid-lowering treatment (yes or no), diabetes (yes or no) and hypertension (yes or no).

Tertiles were based on the distribution of TC levels among controls.

Trend association was assessed by assigning ordinal numbers to each tertile and treating this variable as a continuous variable.

§ p <0.05.

In sensitivity analyses, the exclusion of cases and controls who were undergoing lipid-lowering treatment did not materially change the results (Supplementary Table 2). Similar results were obtained when restricting analyses to cases with two or more TC measurements and their matched controls (data not shown).

Discussion

In the present case-control study nested in a large cohort study of a Japanese working population, we demonstrated that low serum TC levels during the years immediately preceding suicide were significantly associated with an increased risk of suicide death. To our knowledge, this is the first study to demonstrate this association using serum TC levels measured at time points close to suicide death.

A meta-analysis of seven Western cohort studies showed a twofold increased risk of suicide death for the lowest versus highest TC (Wu et al., Reference Wu, Ding, Wu, Xie, Hou and Mao2016). In our study, individuals in the lowest versus highest tertile/predefined category had a three- to four-fold higher risk of suicide death. Our estimates were within the range of relative risks demonstrated in previous prospective studies showing an inverse association between TC and suicide death (approximately two- to six-fold higher risk for the lowest versus highest quartile/predefined category) (Lindberg et al., Reference Lindberg, Råstam, Gullberg and Eklund1992; Neaton et al., Reference Neaton, Blackburn, Jacobs, Kuller, Lee, Sherwin, Shih, Stamler and Wentworth1992; Zureik et al., Reference Zureik, Courbon and Ducimetière1996; Partonen et al., Reference Partonen, Haukka, Virtamo, Taylor and Lonnqvist1999; Ellison & Morrison, Reference Ellison and Morrison2001; Jee et al., Reference Jee, Kivimaki, Kang, Park, Samet and Batty2011). The present study, conducted in a Japanese working population, not only confirms previous findings among primarily Western populations but also provides additional evidence to link suicide risk to TC levels in the recent past.

A Finnish study reported a twofold increased risk of suicide death associated with a TC level ≥309 mg/dl (vs. <193 mg/dl) (Tanskanen et al., Reference Tanskanen, Vartiainen, Tuomilehto, Viinamäki, Lehtonen and Puska2000). A Japanese study showed an approximately twofold increased risk of suicide death associated with a TC level ≥220 mg/dl (vs. 180 to <220 mg/dl) in women but not in men (Svensson et al., Reference Svensson, Inoue, Sawada, Charvat, Mimura and Tsugane2017). In the present study, owing to the presence of few cases with a TC level of ≥240 mg/dl (n=4), we were unable to assess suicide risk associated with high TC levels.

The strengths of this study are its repeated measurements of serum TC levels and the prospective design in a well-defined cohort study. However, there are also some limitations to consider. First, the number of cases (n=41) was not large relative to that of previous studies. Despite this, we observed a statistically significant association between serum TC and the risk of suicide death. Second, we cannot rule out the possibility of bias due to residual confounding effects and unmeasured confounders, such as job stress. Third, as participants were employees at large companies in a Japanese context, we urge caution in generalising the results to populations with different backgrounds.

In conclusion, our findings among a Japanese working population add evidence to support the hypothesis that low serum TC level in the recent past is associated with an increased risk of suicide death. The potential of monitoring serum TC level as a target metric for suicide prevention interventions needs to be further explored.

Supplementary material

To view supplementary material for this article, please visit https://doi.org/10.1017/neu.2019.26.

Author ORCIDs

Chen Sanmei 0000-0003-0811-1701

Acknowledgements

We thank Dr. Toshiteru Okubo (Chairperson of Industrial Health Foundation) for scientific advice on the conduct of J-ECOH Study and Ms. Rika Osawa (National Center for Global Health and Medicine) for administrative supports.

Author contributions

All authors made substantive contributions to the study. SC and TM designed the study. TM and SD designed the J-ECOH project. SC, TM, HH and KK contributed to the statistical analyses. SC, TM, HH, KK, TH, SY, TN, TM, HO, MS, TM, ME, TK, MY, TO, NS, AU, TI, AN, AH, SN, KT, MK, IK and SD collected data and contributed to the interpretations of the data. SC, TM, HH and KK drafted the manuscript. All authors contributed to the revision of the draft. All authors have read and given final approval of the latest version of the manuscript.

Financial support

This study was supported by the Industrial Health Foundation, Industrial Disease Clinical Research Grants (140202-01, 150903-01, 170301-01), Japan Society for the Promotion of Science KAKENHI (16H05251), Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (19K19474); and Grant of National Center for Global Health and Medicine (28-Shi-1206). The funding sources had no role in the study design, collection, analyses and interpretation of the data, writing of the report or the decision to submit the paper for publication.

Conflict of interest

We declared no conflicts of interest relevant to this article.

References

Chang, S, Wen, CP, Tsai, MK, Lawlor, DA, Yang, YC and Gunnell, D (2012) Adiposity, its related biologic risk factors, and suicide: a cohort study of 542,088 Taiwanese adults. The American Journal of Epidemiology 175, 804815.CrossRefGoogle ScholarPubMed
De Berardis, D, Marini, S, Piersanti, M, Cavuto, M, Perna, G, Valchera, A, Mazza, M, Fornaro, M, Iasevoli, F, Martinotti, G and Di Giannantonio, M (2012) The relationships between cholesterol and suicide: an update. ISRN Psychiatry 2012, 16.CrossRefGoogle ScholarPubMed
De Berardis, D, Serroni, N, Campanella, D, Marini, S, Rapini, G, Valchera, A, Iasevoli, F, Mazza, M, Fornaro, M, Perna, G, Di Iorio, G, Martinotti, G and Di Giannantonio, M (2017) Alexithymia, suicide ideation, C-reactive protein, and serum lipid levels among outpatients with generalized anxiety disorder. Archives of Suicide Research 21, 100112.CrossRefGoogle ScholarPubMed
De Berardis, D, Serroni, N, Marini, S, Rapini, G, Carano, A, Valchera, A, Iasevoli, F, Mazza, M, Signorelli, M, Aguglia, E, Perna, G, Martinotti, G, Varasano, PA, Pressanti, GL and Di Giannantonio, M (2014) Alexithymia, suicidal ideation, and serum lipid levels among drug-naïve outpatients with obsessive-compulsive disorder. Revista Brasileira de Psiquiatria 36, 125130.CrossRefGoogle ScholarPubMed
Ellison, LF and Morrison, HI (2001) Low serum cholesterol concentration and risk of suicide. Epidemiology 12, 168172.CrossRefGoogle ScholarPubMed
Giltay, EJ, Zitman, FG, Menotti, A, Nissinen, A, Jacobs, DR, Jr., Adachi, H, Kafatos, A, Kromhout, D, Seven Countries Study Group (2010) Respiratory function and other biological risk factors for completed suicide: 40 years of follow-up of European cohorts of the Seven Countries Study. The Journal of Affective Disorders 120, 249253.CrossRefGoogle ScholarPubMed
Hu, H, Mizoue, T, Sasaki, N, Ogasawara, T, Tomita, K, Nagahama, S, Hori, A, Nishihara, A, Imai, T, Yamamoto, M, Eguchi, M, Kochi, T, Miyamoto, T, Honda, T, Nakagawa, T, Yamamoto, S, Okazaki, H, Uehara, A, Shimizu, M, Murakami, T, Kuwahara, K, Nanri, A, Konishi, M, Kabe, I, Dohi, S, Japan Epidemiology Collaboration on Occupational Health Study Group (2018) Prediabetes and cardiovascular disease risk: a nested case-control study. Atherosclerosis 278, 16.CrossRefGoogle ScholarPubMed
Iribarren, C, Reed, DM, Wergowske, G, Burchfiel, CM and Dwyer, JH (1995) Serum cholesterol level and mortality due to suicide and trauma in the Honolulu Heart Program. Archives of Internal Medicine 155, 695700.CrossRefGoogle ScholarPubMed
Jee, SH, Kivimaki, M, Kang, H-C, Park, IS, Samet, JM and Batty, GD (2011) Cardiovascular disease risk factors in relation to suicide mortality in Asia: prospective cohort study of over one million Korean men and women. European Heart Journal 32, 27732780.CrossRefGoogle ScholarPubMed
Kromhout, D, Katan, M, Menotti, A, Keys, A and Bloemberg, B (1992) Serum cholesterol and long-term death rates from suicide, accidents, or violence. Lancet 340, 317318.CrossRefGoogle ScholarPubMed
Lindberg, G, Råstam, L, Gullberg, B and Eklund, GA (1992) Low serum cholesterol concentration and short term mortality from injuries in men and women. British Medical Journal 305, 277279.CrossRefGoogle ScholarPubMed
Ministry of Health Labour and Welfare of Japan (2018) National Vital Statistics. Available at https://www.mhlw.go.jp/toukei/saikin/hw/jinkou/kakutei17/index.html (accessed 29 November 2018).Google Scholar
Mittendorfer, E and Wasserman, D (2000) Literature Review: Relationship Between Cholesterol and Suicide. Stockholm, Sweden: National Centre for Suicide Research and Prevention of Mental Ill-health.Google Scholar
Neaton, JD, Blackburn, H, Jacobs, D, Kuller, L, Lee, DJ, Sherwin, R, Shih, J, Stamler, J and Wentworth, D (1992) Serum cholesterol level and screened in the Multiple Risk Factor Intervention Trial Group. Archives of Internal Medicine 152, 14901500.CrossRefGoogle Scholar
Partonen, T, Haukka, J, Virtamo, J, Taylor, PR and Lonnqvist, J (1999) Association of low serum total cholesterol with major depression and suicide. British Journal of Psychiatry 175, 239262.CrossRefGoogle ScholarPubMed
Smith, GD, Shipley, MJ, Marmot, MG and Rose, G (1992) Plasma cholesterol concentration and mortality. The Journal of the American Medical Association 267, 7076.CrossRefGoogle ScholarPubMed
Steegmans, PHA, Fekkes, D, Hoes, AW, Bak, AAA, van der Does, E and Grobbee, DE (1996) Low serum cholesterol concentration and serotonin metabolism in men. British Medical Journal 312, 221.CrossRefGoogle ScholarPubMed
Svensson, T, Inoue, M, Sawada, N, Charvat, H, Mimura, M and Tsugane, S (2017) High serum total cholesterol is associated with suicide mortality in Japanese women. Acta Psychiatrica Scandinavica 136, 259268.CrossRefGoogle ScholarPubMed
Tanskanen, A, Vartiainen, E, Tuomilehto, J, Viinamäki, H, Lehtonen, J and Puska, P (2000) High serum cholesterol and risk of suicide. The American Journal of Psychiatry 157, 648650.CrossRefGoogle ScholarPubMed
Wu, S, Ding, Y, Wu, F, Xie, G, Hou, J and Mao, P (2016) Serum lipid levels and suicidality: a meta-analysis of 65 epidemiological studies. The Journal of Psychiatry & Neuroscience 41, 5669.CrossRefGoogle ScholarPubMed
Zureik, M, Courbon, D and Ducimetière, P (1996) Serum cholesterol concentration and death from suicide in men: Paris prospective study I. British Medical Journal 313, 649651.CrossRefGoogle ScholarPubMed
Figure 0

Table 1. Characteristics of suicide cases and controls at 1 year prior to suicide/index date

Figure 1

Table 2. Associations between 3-year average of serum total cholesterol (TC) levels and the risk of suicide death

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