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Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron

Published online by Cambridge University Press:  25 October 2019

Mario A. Gadan ,
Rodrigo Lloyd ,
Gisela Saint Martin ,
María S. Olivera ,
Lucía Policastro  and
Agustina M. Portu
Mario A. Gadan
Affiliation:
Department of Instrumentation and Dosimetry, National Atomic Energy Commission (CNEA), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Institute of Nanoscience and Nanotechnology (INN), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina
Rodrigo Lloyd
Affiliation:
Institute of Nanoscience and Nanotechnology (INN), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Laboratory of Nanomedicine, CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina National Agency for Scientific and Technological Promotion (ANPCyT), Godoy Cruz 2270, C1425FQD, Ciudad Autónoma de Buenos Aires, Argentina
Gisela Saint Martin
Affiliation:
Department of Radiobiology, CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina
María S. Olivera
Affiliation:
Department of Boron Neutron Capture Therapy, CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina
Lucía Policastro
Affiliation:
Institute of Nanoscience and Nanotechnology (INN), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Laboratory of Nanomedicine, CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina National Scientific and Technical Research Council (CONICET), Godoy Cruz 2270, C1425FQD, Ciudad Autónoma de Buenos Aires, Argentina
Agustina M. Portu*
Affiliation:
Institute of Nanoscience and Nanotechnology (INN), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Department of Radiobiology, CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina National Scientific and Technical Research Council (CONICET), Godoy Cruz 2270, C1425FQD, Ciudad Autónoma de Buenos Aires, Argentina
*
*Author for correspondence: A. M. Portu, E-mail: agustina.portu@gmail.com
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Abstract

Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.

Keywords

boron microdistributionboron neutron capture therapy (BNCT)neutron autoradiographypolycarbonate nuclear track detectorUV-C sensitization
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 6 , December 2019 , pp. 1331 - 1340
Copyright
Copyright © Microscopy Society of America 2019 

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