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The complete mitochondrial genome of Orthocoelium streptocoelium (Digenea: Paramphistomidae) for comparison with other digeneans

Published online by Cambridge University Press:  31 March 2016

Y.Y. Zhao ,
X. Yang ,
H.M. Chen ,
L.X. Wang ,
H.L. Feng ,
P.F. Zhao ,
L. Tan ,
W.Q. Lei ,
Y. Ao  and
M. Hu
...Show all authors
Y.Y. Zhao
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
X. Yang
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
H.M. Chen
Affiliation:
Wuchang University of Technology, Wuhan 430223, Hubei, PR China
L.X. Wang
Affiliation:
Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, Hubei, PR China
H.L. Feng
Affiliation:
Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, Hubei, PR China
P.F. Zhao
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
L. Tan
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
W.Q. Lei
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
Y. Ao
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
M. Hu
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
R. Fang*
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
*
*E-mail: fangrui19810705@163.com
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Abstract

Orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. This study first determined the complete mitochondrial (mt) genome of O. streptocoelium. The complete mt genome of O. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. The entire mt genome of O. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except for Opisthorchis viverrini. This mt genome contains 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two non-coding regions. The arrangement of the O. streptocoelium mt genome is the same as those of other digeneans except for Schistosoma haematobium and Schistosoma spindale. Phylogenetic analyses based on concatenated amino acid sequences of the 12 protein-coding genes representing 16 digeneans were conducted to assess the relationship of O. streptocoelium with other digeneans. The result indicated that O. streptocoelium is closely related to Paramphistomum cervi and Fischoederius elongates, which is in accordance with their relationships by taxonomy. This complete mt genome of O. streptocoelium enriched the mitochondrial genome data of paramphistomes and provided important molecular markers for diagnostics and studies of population variation, epidemiology, ecology and evolution of O. streptocoelium and other digeneans.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 2 , March 2017 , pp. 255 - 261
Copyright
Copyright © Cambridge University Press 2016 

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