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Toxic effects of methomyl on mouse oocytes and its possible mechanisms

Published online by Cambridge University Press:  22 October 2021

Daohong He Open the ORCID record for Daohong He [Opens in a new window] ,
Yongnan Xu ,
Lina Hou ,
Jing Wang ,
Shaoying Yang ,
Yu Wang ,
Shurui Zhang ,
Qingguo Jin  and
Qingshan Gao
Daohong He
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Yongnan Xu
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Lina Hou
Affiliation:
Yanbian Korean Autonomous Prefecture Animal Husbandry Master Station, Yanji133002, China
Jing Wang
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Shaoying Yang
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Yu Wang
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Shurui Zhang
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Qingguo Jin
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Qingshan Gao*
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
*
Author for correspondence: Qingshan Gao, College of Agriculture, Yanbian University, Yanji, China. E-mail: qsgao@ybu.edu.cn
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Summary

Methomyl is a broad-spectrum carbamate insecticide that has a variety of toxic effects on humans and animals. However, there have been no studies on the toxicity of methomyl in female mammalian oocytes. This study investigated the toxic effects of environmental oestrogen methomyl exposure on mouse oocyte maturation and its possible mechanisms. Our results indicated that methomyl exposure inhibited polar body extrusion in mouse oocytes. Compared with that in the control group, in the methomyl treatment group, superoxide anion free radicals in oocytes were significantly increased. In addition, the mitochondrial membrane potential of metaphase II stage oocytes in the methomyl treatment group was significantly decreased, resulting in reduced mouse oocyte quality. After 8.5 h of exposure to methomyl, metaphase I stage mouse oocytes displayed an abnormal spindle morphology. mRNA expression of the pro-apoptotic genes Bax and Caspase-3 in methomyl-treated oocytes increased, which confirmed the apoptosis. Collectively, our results indicated that mouse oocyte maturation is defective after methomyl treatment at least through disruption of spindle morphology, mitochondrial function and by induction of oxidative stress.

Keywords

MethomylMitochondriaMouseOocyteOxidative stress
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 358 - 364
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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