Skip to main content Accessibility help

Staurosporine advances interblastomeric flattening of the mouse embryo

  • D. Michael O'Sullivan (a1), Martin H. Johnson (a1) and Josie M.L. McConnell (a1)


Staurosporine, an inhibitor of protein kinase activity, causes premature intercellular flattening of blastomeres but does not induce their premature polarisation. The flattening induced is calcium dependent, is reversed transiently at mitosis and requires the continuing presence of the drug. Staurosporine also blocks the decompacting effect of phorbol ester on 8-cell embryos.


Corresponding author

Professor M.H. Johnson, Embryo and Gamete Research Group, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK. Telephone: (0223) 333789. Fax: (0223) 333786.


Hide All
Bloom, T. (1989). The effects of phorbol ester on mouse blastomeres: a role for protein kinase C in compaction? Development 106, 159–71.
Bloom, T. (1991). Experimental manipulation of compaction of the mouse embryo alters patterns of protein phosphorylation. Mol. Reprod. Dev. 26, 230–44.
Bloom, T. & McConnell, J. (1990). Changes in protein phosphorylation associated with compaction of the early mouse preimplantation embryo. Mol. Reprod. Dev. 26, 199210.
Jefferson, A.B. & Schulman, H. (1988). Sphingosine inhibits calmodulindependent enzymes. J. Biol. Chem. 263, 15241–4.
Johnson, M.H., Chisholm, J.C., Fleming, T.P. & Houliston, E. (1986a). A role for cytoplasmic determinants in the development of the mouse early embryo? J. Embryol. Exp. Morphol. (Suppl.) 97, 97121.
Johnson, M.H., Maro, B. & Takeichi, M. (1986b). The role of cell adhesion in the synchronisation and orientation of polarisation in 8-cell mouse blastomeres. J. Embryol. Exp. Morphol. 93, 239–55.
Kidder, G.M. & McLachlin, J.R. (1985). Timing of transcription and protein synthesis underlying morphogenesis in preimplantation mouse embryos. Dev. Biol. 112, 265–75.
Levy, J.B., Johnson, M.H., Goodhall, H. & Maro, B. (1986). Control of the timing of compaction: a major developmental transition in mouse early development. J. Embryol. Exp. Morphol. 95, 213–37.
Nakadate, T., Yeng, A.Y. & Blumberg, P.M. (1988). Comparison of protein kinase C functional assays to clarify mechanism of inhibitor assay. Biochem. Pharmol 37, 1541–55.
Nasr-Esfahani, M.N., Johnson, M.H. & Aitken, R.J. (1990). The effect of iron and iron chelators on the in vitro block to development of the mouse preimplantation embryo: BAT6, a new medium for improved culture of mouse embryos in vitro. Hum. Reprod. 5, 9971003.
Nicolson, G.G., Yanagimachi, R. & Yanagimachi, H. (1975). Ultrastructural localisation of lectin binding sites of zonae pellucidae and plasma membranes of mammalian eggs. J. Cell Biol. 66, 263–74
Nishizuka, Y. (1986). Studies and perspectives of protein kinase C. Science 233, 305–12.
Nishizuka, Y. (1988). The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334, 661–5.
Oishi, K., Zheng, B. & Kuo, J.F. (1990). Inhibition of Na,K-ATPase and sodium pump by protein kinase C regulations sphingosine, lysophosphatidylcholine and olaic acid. J. Biol. Chem. 265, 70–5.
Poueymirou, W.T. & Schultz, R.M. (1989). Regulation of mouse preimplantation development: inhibition of synthesis of proteins in the two-cell embryo that require transcription. Development 133, 588–99.
Sefton, M., Johnson, M.H. & Clayton, L.C. (1992). Synthesis and phosphorylarion of uvomorulin during mouse early development. Development 115, 313–18.
Shirayoshi, Y., Okada, T.S. & Takeichi, M. (1983). The calcium-dependent cell-cell adhesion system regulates inner cell mass formation and cell surface polarisation in early mouse development. Cell 35, 631–8.
Smith, R.K.F. & Johnson, M.H. (1985).DNA replication and compaction in the cleaving embryo of the mouse. J. Embryol. Exp. Morphol. 89, 133–48.
Tamaoki, T. (1991). Use and specificity of staurosporine, UCN–01, and calphostin C as protein kinase inhibitors. Methods Enzymol 201, 340–7.
Tamaoki, T., Nomoto, H., Takahashi, I., Yuzuru, K., Makoto, M. & Pusao, T. (1986). Staurosporine, a potent inhibitor or phospholipids/Ca++ dependent protein kinase. Biochem. Biophys. Res. Commun. 135, 397402.
Yamamura, H. & Spindle, A. (1988). Stage-specific response of preimplantation mouse embryos to W-7, a calmodulin antagonist. J. Exp. Zool. 248, 4554.
Yamamura, H., Ohsugi, M., Ohta, H., Naka, M. & Hidaka, H. (1987). Compaction-like aggregation of blastomeres by 1–oleoyl-2-acetylglycerol in mouse embryos. Jpn. Teratol. Abstr. 36, 445.
Yamamura, H., Ohta, H., Ohsuga, M., Takagishyi, Y. & Hidaka, H. (1989). Possible involvement of protein kinase C in compaction of preimplantation mouse embryos. Cell Differ. Dev. (Suppl.) 27, S119.
Winkel, G.K., Ferguson, J.E., Takeichi, M. & Nuccitelli, R. (1990). Activation of protein kinase C triggers premature compaction in the four-cell stage mouse embryo. Dev. Biol. 138, 115.


Staurosporine advances interblastomeric flattening of the mouse embryo

  • D. Michael O'Sullivan (a1), Martin H. Johnson (a1) and Josie M.L. McConnell (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed