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Sperm characterization of the Amazonian freshwater cururu stingray Potamotrygon wallacei (Potamotryogonidae): basic knowledge for reproduction and conservation plans

Published online by Cambridge University Press:  09 August 2019

Ruben Dario Morales-Gamba ,
Jôsie Schwartz Caldas ,
Leandro Godoy  and
Jaydione Luiz Marcon Open the ORCID record for Jaydione Luiz Marcon [Opens in a new window]
Ruben Dario Morales-Gamba
Affiliation:
Graduate Programme in Aquaculture, Nilton Lins University/National Institute of Amazonian Research, Manaus, Brazil
Jôsie Schwartz Caldas
Affiliation:
Graduate Programme in Aquaculture, Nilton Lins University/National Institute of Amazonian Research, Manaus, Brazil
Leandro Godoy
Affiliation:
Graduate Programme in Aquaculture, Nilton Lins University/National Institute of Amazonian Research, Manaus, Brazil Department of Animal Science, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
Jaydione Luiz Marcon*
Affiliation:
Graduate Programme in Aquaculture, Nilton Lins University/National Institute of Amazonian Research, Manaus, Brazil Department of Physiological Sciences, Federal University of Amazonas (UFAM), Manaus, Brazil.
*
Address for correspondence: Jaydione Luiz Marcon. Laboratory of Animal Physiology, Department of Physiological Sciences, Federal University of Amazonas (UFAM), Av. General Rodrigo Octávio, 6200 – Coroado I, 69080-900, Manaus – AM, Brazil. Tel: +55 92 3305. E-mail: jlmarcon@ufam.edu.br
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Summary

This study aimed to describe the morphology and sperm quality of free-living adult males of cururu stingray Potamotrygon wallacei, endemic from the Rio Negro basin, Brazilian Amazon. The sperm was collected in loco from the seminal vesicle region and fixed in buffered saline formaldehyde solution for further evaluation of morphometry, sperm plasma membrane integrity and sperm concentration. The spermatozoa presented a total length of 138.25 ± 1.82 μm with a helical shape and a long head. A high percentage of cells with intact membrane (98 ± 2%) and normal spermatozoa (92 ± 1%) were observed. The cell concentration was 0.34 ± 0.05 × 1010 spermatozoa/ml of semen. These observations are unprecedented for potamotrygonid species and will serve as a basis for future management and conservation strategies.

Keywords

ElasmobranchsFreshwater stingrayMorphology of spermatozoaSperm abnormalitySperm plasma membrane integrity
Type
Short Communication
Information
Zygote , Volume 27 , Issue 4 , August 2019 , pp. 259 - 261
Copyright
© Cambridge University Press 2019 

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