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Spatial distribution of preantral follicles in ovarian parenchyma of bovine species

Published online by Cambridge University Press:  16 February 2023

Suellen Miguez González ,
Marcela Bortoletto Cerezetti ,
Larissa Zamparone Bergamo Open the ORCID record for Larissa Zamparone Bergamo [Opens in a new window] ,
Fábio Morotti  and
Marcelo Marcondes Seneda Open the ORCID record for Marcelo Marcondes Seneda [Opens in a new window]
Suellen Miguez González
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics, DCV; Center of Agrarian Sciences, CCA; State University of Londrina, UEL; Londrina, Parana, Brazil
Marcela Bortoletto Cerezetti
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics, DCV; Center of Agrarian Sciences, CCA; State University of Londrina, UEL; Londrina, Parana, Brazil
Larissa Zamparone Bergamo
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics, DCV; Center of Agrarian Sciences, CCA; State University of Londrina, UEL; Londrina, Parana, Brazil
Fábio Morotti
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics, DCV; Center of Agrarian Sciences, CCA; State University of Londrina, UEL; Londrina, Parana, Brazil
Marcelo Marcondes Seneda*
Affiliation:
Laboratory of Biotechnology of Animal Reproduction; Department of Veterinary Clinics, DCV; Center of Agrarian Sciences, CCA; State University of Londrina, UEL; Londrina, Parana, Brazil
*
Author for correspondence: Marcelo Marcondes Seneda. Laboratory of Biotechnology of Animal Reproduction, Center of Agrarian Sciences, CCA, State University of Londrina, UEL, Londrina, Parana, Brazil 86057-970. Tel: +55 43 3371 5622. E-mail: marcelo.seneda@uel.br
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Summary

The goal of this study was to determine the distribution of preantral follicles in bovine ovaries. Follicular distribution in the ovaries (n = 12) was evaluated in the region of the greater curvature of the ovary (GCO) and the region close to the ovarian pedicle (OP) of Bos taurus indicus heifers of the Nelore breed. Two fragments were obtained from each region of the ovary (GCO and OP). The mean weight of the ovaries was 4.04 ± 0.32 g. The mean antral follicle count (AFC) was 54.58 ± 3.55 follicles (minimum and maximum variation of 30 and 71 follicles, respectively). In total, 1123 follicles were visualized in the region of the GCO; 949 (84.5%) of them were primordial follicles and 174 (15.5%) were developing follicles. The region close to the OP contained 1454 follicles, of which 1266 (87%) were primordial follicles and 44 (12.9%) were developing follicles. The OP region showed a higher proportion of intact follicles in the primordial (P < 0.0001) and primary (P = 0.042) stages compared with the GCO region. The proportion of secondary follicles was similar in the OP and GCO regions. The ovaries of two bovine females (16%; 2/12) contained multi-oocytes follicles, which were characterized as primary follicles. Therefore, the distribution of preantral follicles in the bovine ovary was heterogeneous, with the region close to the OP containing a greater number of preantral follicles compared with the GCO region (P < 0.05).

Keywords

Bos taurus indicusFollicular locationFollicular reserveOvarian folliclesOvarian region
Type
Research Article
Information
Zygote , Volume 31 , Issue 2 , April 2023 , pp. 195 - 200
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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