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Production of in vitro bovine embryos supplemented with l-carnitine in different oxygen tensions and the relation to nitric oxide

Published online by Cambridge University Press:  23 July 2020

Daniela Moraes Pereira ,
Christopher Junior Tavares Cardoso ,
Wilian Aparecido Leite da Silva ,
Mirela Brochado Souza-Cáceres ,
Mariana Santos Open the ORCID record for Mariana Santos [Opens in a new window] ,
Ralf Pöhland ,
Allan Motta Couto ,
Iluska Senna Bonfá Moslaves ,
Mônica Cristina Toffoli Kadri  and
Fabiana de Andrade Melo Sterza Open the ORCID record for Fabiana de Andrade Melo Sterza [Opens in a new window]
Daniela Moraes Pereira
Affiliation:
State University of Mato Grosso do Sul, Animal Science, Aquidauana, Mato Grosso do Sul, Brazil
Christopher Junior Tavares Cardoso
Affiliation:
Federal University of Mato Grosso do Sul, Veterinary Science, Campo Grande, Mato Grosso do Sul, Brazil
Wilian Aparecido Leite da Silva
Affiliation:
Federal University of Mato Grosso do Sul, Animal Science, Campo Grande, Mato Grosso do Sul, Brazil
Mirela Brochado Souza-Cáceres
Affiliation:
State University of Londrina, Animal Science, Londrina, Paraná, Brazil
Mariana Santos
Affiliation:
State University of Mato Grosso do Sul, Animal Science, Aquidauana, Mato Grosso do Sul, Brazil
Ralf Pöhland
Affiliation:
State University of Londrina, Animal Science, Londrina, Paraná, Brazil
Allan Motta Couto
Affiliation:
State University of Mato Grosso do Sul, Animal Science, Aquidauana, Mato Grosso do Sul, Brazil
Iluska Senna Bonfá Moslaves
Affiliation:
Federal University of Mato Grosso do Sul, Pharmacology and Inflammation Laboratory, Campo Grande, Mato Grosso do Sul, Brazil
Mônica Cristina Toffoli Kadri
Affiliation:
Federal University of Mato Grosso do Sul, Pharmacology and Inflammation Laboratory, Campo Grande, Mato Grosso do Sul, Brazil
Fabiana de Andrade Melo Sterza*
Affiliation:
State University of Mato Grosso do Sul, Animal Science, Aquidauana, Mato Grosso do Sul, Brazil Federal University of Mato Grosso do Sul, Veterinary Science, Campo Grande, Mato Grosso do Sul, Brazil Leibniz Institute for Farm Animal Biology, Institute of Reproductive Biology, Dummerstorf, Germany
*
Author for correspondence: Fabiana de Andrade Melo Sterza. Universidade Estadual de Mato Grosso do Sul – Aquidauana/MS. 25 Rodovia Graziela Maciel Barrozo, Km 12 Zona Rural, Aquidauana – MS, CEP: 79200-000, Brazil. Tel: +55 67 39042242. E-mail: fabiana.sterza@uems.br
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Summary

The aim of this study was to evaluate the production of bovine embryos in vitro when supplemented with l-carnitine for 24 h beginning on day 5 (d 5) under two different oxygen tensions (20% or 5%) and the relationship of nitric oxide (NO) in in vitro culture (IVC) medium to embryo development. Cumulus–oocyte complexes (COC; n = 837) were matured in vitro for 24 h and fertilization was performed for 18 h. Zygotes were cultured in vitro for 9 days after in vitro fertilization in synthetic oviductal fluid (SOF) medium with 5% fetal calf serum. At d 5 the plates were assigned to one of four treatment groups: high (20%) or low (5%) O2 tension either with or without the addition of 3.03 mM l-carnitine (High-Cont, High-Lcar, Low-Cont, Low-Lcar). The concentration of NO in the culture medium was evaluated on d 5, d 6 and d 9. On d 7, parts of the embryos were submitted for evaluation of intracellular lipid droplets. The cleavage rate was similar (P > 0.05) between high and low O2 tension and the blastocyst rate was similar in all conditions evaluated. The hatching rate was higher (P < 0.05) for Low-Cont. The NO concentration was higher at d 9 under low O2 tension (P < 0.1). The addition of 3.03 mM l-carnitine between d 5 and d 6 of IVC was not efficient in reducing cytoplasmic lipid content of bovine embryos. Additionally, IVC at a low oxygen tension without l-carnitine promoted better conditions for embryo development. A higher concentration of NO in medium was observed under low O2 tension.

Keywords

BovineEmbryo developmentNile RedReactive nitrogen species
Type
Research Article
Information
Zygote , Volume 28 , Issue 5 , October 2020 , pp. 403 - 408
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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