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PKA activation in concert with ARIS and asterosap induces the acrosome reaction in starfish

Published online by Cambridge University Press:  01 November 2006

M. Sadiqul Islam ,
O. Kawase ,
S. Hase ,
M. Hoshi  and
M. Matsumoto
M. Sadiqul Islam
Affiliation:
Department of Biosciences and Informatics, Keio University 3–14–1, Hiyoshi, Kouhoku-ku, Yokohama, Japan.
O. Kawase
Affiliation:
Department of Biosciences and Informatics, Keio University 3–14–1, Hiyoshi, Kouhoku-ku, Yokohama, Japan.
S. Hase
Affiliation:
Department of Biology, Keio University 4–1–1, Hiyoshi, Kouhoku-ku, Yokohama, Japan.
M. Hoshi
Affiliation:
Department of Biosciences and Informatics, Keio University 3–14–1, Hiyoshi, Kouhoku-ku, Yokohama, Japan.
M. Matsumoto*
Affiliation:
Department of Biosciences and Informatics, Keio University 3–14–1, Hiyoshi, Kouhoku-ku, Yokohama, Japan.
*
All correspondence to: M. Matsumoto. Department of Biosciences and InformaticsKeio University 3–14–1, Hiyoshi, Kouhoku-ku, Yokohama 223–8522, Japan. Tel: +81 045 566 1774. Fax: +81 045 566 1448. e-mail: mmatsumo@bio.keio.ac.jp
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Summary

The acrosome reaction (AR) is a fundamental event for fertilization, which is induced in concert with acrosome reaction-inducing substance (ARIS) and asterosap, both of which are components of starfish egg jelly (EJ). During the AR, a spermatozoon undergoes a series of physiological changes, such as in intracellular cGMP concentration ([cGMP]i), pHi and intracellular Ca2+ concentration ([Ca2+]i). Affinity purification of cGMP-binding protein resulted in the isolation of a regulatory subunit of the cAMP-dependent protein kinase A (PKA), suggesting the involvement of a cAMP-dependent pathway in the AR. By using a cAMP enzyme immunoassay, [cAMP]i was found to increase in starfish spermatozoa when stimulated with ARIS and asterosap. ARIS could also increase the [cAMP]i in the presence of high pH seawater. Pretreatment of spermatozoa with two specific and cell-permeable PKA inhibitors, H89 and KT5720, prevented the induction of the AR in a concentration-dependent manner. These results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap. To investigate this, we have cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.

Keywords

Acrosome reactionCyclic AMPEgg jellyPKAStarfish
Type
Research Article
Information
Zygote , Volume 14 , Issue 4 , November 2006 , pp. 329 - 340
Copyright
Copyright © Cambridge University Press 2006

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