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Partial formation of sperm dimorphism from spermatocytes of the cottoid fish, Hemilepidotus gilberti in cell culture

Published online by Cambridge University Press:  01 November 2007

Y. Hayakawa ,
E. Takayama-Watanabe ,
A. Watanabe ,
M. Kobayashi ,
H. Munehara  and
K. Onitake
Y. Hayakawa
Affiliation:
Department of Biology, Division of Natural Sciences, International Christian University, 3-10-2 Osawa, Mitaka, Tokyo, 181-8585Japan.
E. Takayama-Watanabe
Affiliation:
Yamagata Junior College, 515 Katayachi, Yamagata, 990-2316Japan.
A. Watanabe*
Affiliation:
Department of Biology, Faculty of Science, Yamagata University, 1–4-12 Kojirakawa, Yamagata, 990–8560, Japan.
M. Kobayashi
Affiliation:
Department of Biology, Division of Natural Sciences, International Christian University, 3-10-2 Osawa, Mitaka, Tokyo, 181-8585Japan.
H. Munehara
Affiliation:
Usujiri Fisheries Station, Field Science Center for Northern Biosphere, Hokkaido University, Hakodate, Hokkaido, 041–1613, Japan.
K. Onitake
Affiliation:
Department of Biology, Faculty of Science, Yamagata University, 1–4-12 Kojirakawa, Yamagata, 990–8560, Japan.
*
All correspondence to: Akihiko Watanabe, Department of Biology, Faculty of Science, Yamagata University, 1-4-12 Kojirakawa, Yamagata, 990-8560, Japan. Tel:/Fax: +81 23 628 4619. e-mail: watan@sci.kj.yamagata-u.ac.jp
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Summary

Polymorphism of sperm is considered to be significant for the reproductive strategy in some animal species. The phenomenon is thought to occur in the species-specific stage of spermatogenesis, but how the identical germ cells are differentiated towards polymorphic sperm remains unknown. We here performed a germ cell culture in the cottoid fish, Hemilepidotus gilberti, whose sperm exhibit dimorphism with fertilizable eusperm and unfertilizable parasperm. In the culture, germ cells, which were obtained with an identical morphology, a spherical shape of 5–7 µm in diameter, differentiated into smaller spherical cells with a single nucleus, a moving flagellum and localized mitochondria. In addition, large retroflex-shaped cells with two elongated nuclei were also observed in the cell culture. Germ cells that had each morphological feature were histologically also observed in some cysts of the spermatogenetic testis, suggesting that the former type of cell corresponded to developing eusperm and the latter corresponded to developing parasperm. When BrdU was incorporated into germ cells in the culture, it was detected in both cells with eusperm-like and those with parasperm-like morphologies. These findings suggest that DNA-duplicating spermatocytes are potent to autonomously progress a part of spermatogenesis to form dimorphic sperm.

Keywords

Cottoid fishDimorphic spermIn vitro spermatogenesisParaspermSpermiogenesis
Type
Research Article
Information
Zygote , Volume 15 , Issue 4 , November 2007 , pp. 285 - 293
Copyright
Copyright © Cambridge University Press 2007

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