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Mouse oocytes penetrated by sperm at GV or GVBD stage lose the ablity to fuse with additional spermatozoa

Published online by Cambridge University Press:  26 September 2008

Beata Pyrzyńska ,
M. Maleszewski  and
D. Maluchnik
Beata Pyrzyńska
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
M. Maleszewski
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
D. Maluchnik*
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Warsaw, Poland
*
Marek Maleszewski, Department of Embryology, Institute of Zoology, University of Warsaw, 00-927 Warszawa 64, Poland. Fax: (48 22) 26 86 24. e-mail:embrio@plearn.edu.pl.
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Summary

Mouse oocytes penetrated by spermatozoa during germinal vesicle (GV) breakdown undergo maturation and are arrested at metaphase of the second meiotic division despite the presence of sperm nuclei within the ooplasm. When these oocytes were re-inseminated, none was penetrated by newly added spermatozoa. When GV oocytes were inseminated and cultured in the presence of dibutyryl cAMP, the oocytes remained at GV stage, yet they did not permit entry of additional spermatozoa. These observations suggest that the plasma membrane of maturing oocytes is modified by precociously penetrating spermatozoa independently from cortical granule exocytosis. Sperm components incorporated into the oocytes seem to be responsible for the modification of the oocyte's plasma membrane.

Keywords

Mouse oocyteOocte maturationOolemma block to sperm penetrationIn vitro fertilisationRe-insemination
Type
Article
Information
Zygote , Volume 4 , Issue 2 , May 1996 , pp. 123 - 128
Copyright
Copyright © Cambridge University Press 1996

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