Skip to main content Accessibility help
×
×
Home

Kit ligand promotes the transition from primordial to primary follicles after in vitro culture of ovine ovarian tissue

  • A.Y.P. Cavalcante (a1), B.B. Gouveia (a1), R.S. Barberino (a1), T.L.B.G. Lins (a1), L.P. Santos (a1), R.J.S. Gonçalves (a1), J.J.H. Celestino (a2) and M.H.T. Matos (a3) (a1)...

Summary

This study evaluated the effects of kit ligand (KL) on the morphology and development of ovine preantral follicles (fresh control) and after 7 days of in vitro culture in α-Minimal Essential Medium (α-MEM; control medium) or the presence of KL (1, 10, 50, 100 or 200 ng/ml). There was an increase in the percentage of primary follicles at the concentration of 100 ng/ml KL, compared with the fresh control, control medium (α-MEM) and the other KL concentrations. Follicle diameter was significantly higher than the control medium only at concentrations of 50 and 100 ng/ml KL. In conclusion, 100 ng/ml KL promoted the transition from primordial to primary follicles (follicular activation) after in vitro culture of ovine ovarian tissue.

Copyright

Corresponding author

All correspondence to: M.H.T. Matos. Universidade Federal do Vale do São Francisco (UNIVASF), Campus de Ciências Agrárias. Colegiado de Medicina Veterinária – Laboratório de Biologia Celular, Citologia e Histologia, Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1, CEP: 56300-990, Petrolina, PE, Brasil. Tel: +55 87 2101 4839. E-mail: helena.matos@univasf.edu.br

References

Hide All
Adhikari, D. & Liu, K. (2009). Molecular mechanisms underlying the activation of mammalian primordial follicles. Endocr. Ver. 30, 438–64.
Celestino, J.J.H., Bruno, J.B., Lima-Verde, I.B., Matos, M.H.T., Saraiva, M.V.A., Chaves, R.N., Martins, F.S., Lima, L.F., Name, K.P.O., Campello, C.C., Silva, J.R.V., Báo, S.N., & Figueiredo, J.R. (2009). Recombinant epidermal growth factor maintains follicular ultrastructure and promotes the transition to primary follicles in caprine ovarian tissue cultured in vitro . Reprod. Sci. 16, 239–46.
Celestino, J.J.H., Bruno, J.B., Lima-Verde, I.B., Matos, M.H.T., Saraiva, M.V.A., Chaves, R.N., Martins, F.S., Almeida, A.P., Cunha, R.M.S., Lima, L.F., Name, K.O., Campello, C.C., Silva, J.R.V., Báo, S.N. & Figueiredo, J.R. (2010). Steady-state level of kit ligand mRNA in goat ovaries and the role of kit ligand in preantral follicle survival and growth in vitro . Mol. Reprod. Dev. 77, 231–40.
Chaves, R.N., Martins, F.S., Saraiva, M.V.A., Celestino, J.J.H., Lopes, C.A.P., Correia, J.C., Lima-Verde, I.B., Matos, M.H.T., Báo, S.N., Name, K.P.O., Campello, C.C., Silva, J.R.V. & Figueiredo, J.R. (2008). Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro . Reprod. Fertil. Dev. 20, 640–7.
Esmaielzadeh, F., Hosseini, S.M., Nasiri, Z., Hajian, M., Chamani, M., Gourabi, H., Shahverdi, A.H., Vosough, A.D. & Nasr-Esfahani, M.H. (2013). Kit ligand and glial-derived neurotrophic factor as alternative supplements for activation and development of ovine preantral follicles in vitro . Mol. Reprod. Dev. 80, 3547.
Faustino, L.R., Lima, I.M.T., Carvalho, A.A., Silva, C.M.G., Castro, S.V., Lobo, C.H., Lucci, C.M., Campello, C.C., Figueiredo, J.R. & Rodrigues, A.P.R. (2013). Interaction between keratinocyte growth factor-1 and kit ligand on goat preantral follicles cultured in vitro . Small Rumin. Res. 114, 112–9.
Hutt, K.J., Mclaughlin, E.A. & Holland, M.K. (2006). KIT/KIT Ligand in mammalian oogenesis and folliculogenesis: roles in rabbit and murine ovarian follicle activation and oocyte growth. Biol. Reprod. 75, 421–33.
Jin, X., Han, C.S., Yu, F.Q., Wei, P., Hu, Z.Y. & Liu, Y.X. (2005). Anti-apoptotic action of stem cell factor on oocytes in primordial follicles and its signal transduction. Mol. Reprod. Dev. 70, 8290.
Lima, I.M.T., Celestino, J.J.H., Faustino, L.R., Magalhães-Padilha, D.M., Rossetto, R., Brito, I.R., Donato, M.A.M., Lopes, C.A.P., Campello, C.C., Peixoto, C.A., Figueiredo, J.R. & Rodrigues, A.P.R. (2012). Dynamic medium containing kit ligand and follicle-stimulating hormone promotes follicular survival, activation, and growth during long-term in vitro culture of caprine preantral follicles. Cells Tissues Org. 195, 260–71.
Martins, F.S., Celestino, J.J. H., Saraiva, M.V.A., Matos, M.H.T., Bruno, J.B.; Rocha-Junior, C.M.C., Lima-Verde, I.B., Lucci, C.M., Báo, S.N. & Figueiredo, J.R. (2008). Growth and differentiation factor-9 stimulates activation of goat primordial follicles in vitro and their progression to secondary follicles. Reprod. Fertil. Dev. 20, 916–24.
Martins, F.S., Celestino, J.J.H., Saraiva, M.V.A., Chaves, R.N., Rossetto, R., Silva, C.M.G., Lima-Verde, I.B., Lopes, C.A.P., Campello, C.C. & Figueiredo, J.R. (2010). Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles. Braz. J. Med. Biol. Res. 43, 728–36.
Muruvi, W., Picton, H.M., Rodway, R.G. & Joyce, I.M. (2005). In vitro growth of oocytes from primordial follicles isolated from frozen–thawed lamb ovaries. Theriogenology 64, 1357–70.
Nilsson, E.E. & Skinner, M.K. (2004). Kit ligand and basic fibroblast growth factor interactions in the induction of ovarian primordial to primary follicle transition. Mol. Cell. Endocrinol. 214, 1925.
Nobrega, J.E. Jr, Gonçalves, P.B.D., Chaves, R.N., Magalhães, D.M., Rossetto, R., Lima-Verde, I.B., Pereira, G.R., Campello, C.C., Figueiredo, J.R. & Oliveira, J.F.C. (2011). Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro . Zygote 20, 73–8.
Otsuka, F. & Shimasaki, S. (2002). A negative feedback system between oocyte bone morphogenetic protein 15 and granulosa cell kit ligand: Its role in regulating granulosa cell mitosis. Proc. Natl. Acad. Sci. USA 99, 8060–5.
Reddy, P., Shen, L., Ren, C., Boman, K., Lundin, E., Ottander, U., Lindgren, P., Liu, Y-x, Sun, Q-Y. & Liu, K. (2005). Activation of Akt (PKB) and suppression of FKHRL1 in mouse and rat oocytes by stem cell factor during follicular activation and development. Dev. Biol. 281, 160–70.
Reynaud, K., Cortvrindt, R., Smitz, J. & Driancourt, M.A. (2000). Effects of kit ligand and anti-kit antibody on growth of cultured mouse preantral follicles. Mol. Reprod. Dev. 56, 483–94.
Ribeiro, R.P., Portela, A.M.L.R., Silva, A.W.B., Costa, J.J.N., Passos, J.R.S., Cunha, E.V., Souza, G.B., Saraiva, M.V.A., Donato, M.A.A., Peixoto, C.A., Hurk, R.V.D. & Silva, J.R.V. (2014). Effects of jacalin and follicle-stimulating hormone on in vitro goat primordial follicle activation, survival and gene expression. Zygote 29, 113.
Rosseto, R., Lima-Verde, I.B., Matos, M.H.T., Saraiva, M.V.A. & Martins, F.S. (2009). Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles. Domest. Anim. Endocrinol. 37, 112–3.
Wang, J. & Roy, S.K. (2004). Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone. Biol. Reprod. 70, 577–85.
Wongsrikeao, P., Otoi, T., Karja, N.W.K., Agung, B., Nii, M. & Nagai, T. (2005). Effects of ovary time and temperature on DNA fragmentation and development of porcine oocytes. J. Reprod. Dev. 51, 8797.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Zygote
  • ISSN: 0967-1994
  • EISSN: 1469-8730
  • URL: /core/journals/zygote
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed