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Investigation of histone H4 hyperacetylation dynamics in the 5S rRNA genes family by chromatin immunoprecipitation assay

Published online by Cambridge University Press:  15 October 2014

Liliana Burlibașa  and
Ilinca Suciu
Liliana Burlibașa*
Affiliation:
University of Bucharest, Genetics Department, Bucharest, Romania. University of Bucharest, Genetics Department, Bucharest, Romania.
Ilinca Suciu
Affiliation:
University of Bucharest, Genetics Department, Bucharest, Romania.
*
All correspondence to: Liliana Burlibașa. University of Bucharest, Genetics Department, Bucharest, Romania. e-mail: liliana_burlibasa@yahoo.com.au
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Summary

Oogenesis is a critical event in the formation of female gamete, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes dramatically concomitant with genome remodelling, while genomic information is stably maintained. The aim of the present study was to investigate the presence of H4 acetylation of the oocyte and somatic 5S rRNA genes in Triturus cristatus, using chromatin immunoprecipitation assay (ChIP). Our findings suggest that some epigenetic mechanisms such as histone acetylation could be involved in the transcriptional regulation of 5S rRNA gene families.

Keywords

5S rDNAoocytesChIP assayH4 hyperacetylationTriturus cristatus
Type
Short Communication
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 951 - 954
Copyright
Copyright © Cambridge University Press 2014 

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