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In vitro effect of various cryoprotectants on the semen quality of endangered Oravka chicken

Published online by Cambridge University Press:  12 December 2017

Andrea Svoradová ,
Lenka Kuželová ,
Jaromír Vašíček ,
Andrej Baláži ,
Emília Hanusová  and
Peter Chrenek
Andrea Svoradová*
Affiliation:
Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic.
Lenka Kuželová
Affiliation:
Slovak University of Agriculture in Nitra, Research Centre AgroBioTech, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic.
Jaromír Vašíček
Affiliation:
Slovak University of Agriculture in Nitra, Research Centre AgroBioTech, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic. Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic.
Andrej Baláži
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic.
Emília Hanusová
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic.
Peter Chrenek
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic. Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic.
*
All correspondence to: Andrea Svoradová. Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic. Tel: +421 0376408720. E-mail: andrea.svoradova@ukf.sk
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Summary

We aimed to compare the effect of three different permeating cryoprotectants on the post-thaw spermatozoa quality. Pooled semen from Oravka cock line (n = 6) was diluted in Kobidil+ extender and frozen in cryoprotectant solutions containing 8% dimethylsulfoxide (DMSO), 8% ethylene glycol (EG) or 8% glycerol (GL) in liquid nitrogen vapours before being plunged into the liquid nitrogen. Spermatozoa motility parameters were assessed in vitro after freezing–thawing by a computer-assisted semen analysis (CASA) system and viability status was examined using fluorescent probes. The lower percentage (P < 0.05) of motile and progressively moving spermatozoa immediately after thawing were obtained in all experimental groups (DMSO, EG, GL) compared with the control. Significant (P < 0.05) differences in total motility and progressive movement between GL and DMSO, EG groups were observed. However, the higher number (P < 0.05) of acrosome damaged spermatozoa was found in the DMSO and EG groups and no significant differences were observed in the GL group compared with the control. Differences (P < 0.05) between experimental groups and the control in the results of spermatozoa necrosis were observed. No significant differences in the percentage of apoptotic spermatozoa were found between control and experimental groups. However, significant differences (P < 0.05) in number of live and necrotic spermatozoa between GL and DMSO, EG groups were examined. The findings of the present study indicate that glycerol seems to be suitable for semen cryopreservation in the gene banks. In addition, fertility evaluation in vivo is needed in order to evaluate the possible contribution for the bank of animal genetic resources.

Keywords

CryopreservationMotilityOravka breedSemenViability
Type
Research Article
Information
Zygote , Volume 26 , Issue 1 , February 2018 , pp. 33 - 39
Copyright
Copyright © Cambridge University Press 2017 

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