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Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

  • Danuza L. Leão (a1), Adriel B. Brito (a1), Stefânia A. Miranda (a1), Karol G. Oliveira (a1) (a2), Débora V.C. Almeida (a1), Regiane R. Santos (a3) and Sheyla F. S. Domingues (a1)...


We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.


Corresponding author

All correspondence to: Regiane R Santos. Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. E-mail:


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Agarwal, A., Sharma, R.K., Nallella, K.P., Thomas, A.J. Jr., Alvarez, J.G. & Sikka, S.C. (2006). Reactive oxygen species as an independent marker of male factor infertility. Fertil. Steril. 86, 878–85.
Agarwal, A., Virk, G., Ong, C. & du Plessis, S.S. (2014). Effect of oxidative stress on male reproduction. World J. Men's Health 32, 117.
Aitken, R.J. & Baker, M.A. (2006). Oxidative stress, sperm survival and fertility control. Mol. Cel. Endocrinol. 250, 66–9.
Dixson, A.F. & Anderson, M.J. (2002). Sexual selection, seminal coagulation and copulatory plug formation in Primates. Folia Primatol. 73, 63–9.
Dong, Q., Rodenburg, S.E., Huang, C. & VandeVoort, C.A. (2008). Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. J. Androl. 29, 283–92
Dong, Q., Correa, L.M. & Yandevoort, C.A. (2009). Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant. Cryobiology 58, 20–7.
Kefer, J.C., Agarwal, A. & Sabanegh, E. (2009). Role of antioxidants in the treatment of male infertility. Int. J. Urol. 16, 449–57.
Kothari, S., Thompson, A., Agarwal, A. & Plessis, S.S. (2010). Free radicals: their beneficial and detrimental effects on sperm function. Indian J. Exp. Biol. 48, 425–35.
Leão, D.L., Miranda, S.A., Brito, A.B., Lima, J.S., Santos, R.R. & Domingues, S.F.S. (2015). Efficacious long-term cooling and freezing of I semen in ACP-118®. Anim. Reprod. Sci. 159, 118–23.
McCarthy, M.J. & Meyers, S.A. (2011). Antioxidant treatment in the absence of exogenous lipids and proteins protects rhesus macaque sperm from cryopreservation-induced cell membrane damage. Theriogenology 76, 168–76.
Mora-Esteves, C. & Shin, D. (2013). Nutrient supplementation: improving male fertility fourfold. Sem. Reprod. Med. 31, 293300.
Moubasher, A.E., El Din, A.M.E., Ali, M.E., El-Sherif, W.T. & Gaber, H.D. 2013. Catalase improves motility, vitality and DNA integrity of cryopreserved human spermatozoa. Andrologia 45, 135–9.
Oliveira, K.G., Miranda, S.A., Leão, D.L., Brito, A.B., Santos, R.R. & Domingues, S.F.S. (2011). Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES–TRIS. Anim. Reprod. Sci. 123, 7580.
Oliveira, K.G., Leão, D.L., Almeida, D.V.C., Santos, R.R & Domingues, S.F.S. (2015). Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi . Theriogenology 84, 743–9.
Oliveira, K.G., Santos, R.R., Leão, D.L., Queiroz, H.L., Paim, F.P., Vianez-Júnior, J.L.S.G. & Domingues, S.F.S. (2016a). Similarities in testicular and seminal aspects in four squirrel monkeys’ species. Theriogenology 86, 879–87.
Oliveira, K.G., Santos, R.R., Leão, D.L., Brito, A.B., Lima, J.S., Sampaio, W.V. & Domingues, S.F.S. (2016b). Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species. Cryobiology 72, 283–9.
Taylor, K., Roberts, P., Sanders, K. & Burton, P. (2009). Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa. Reprod. BioMed. Online 18, 184–9.


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