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Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

  • Danuza L. Leão (a1), Adriel B. Brito (a1), Stefânia A. Miranda (a1), Karol G. Oliveira (a1) (a2), Débora V.C. Almeida (a1), Regiane R. Santos (a3) and Sheyla F. S. Domingues (a1)...

Summary

We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.

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Corresponding author

All correspondence to: Regiane R Santos. Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. E-mail: R.Rodriguesdossantos@pq.cnpq.br

References

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