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Expression of voltage-activated calcium channels in the early zebrafish embryo

Published online by Cambridge University Press:  01 May 2009

Dayán Sanhueza ,
Andro Montoya ,
Jimena Sierralta  and
Manuel Kukuljan
Dayán Sanhueza
Affiliation:
Program in Physiology and Biophysics, Institute for Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile.
Andro Montoya
Affiliation:
Program in Physiology and Biophysics, Institute for Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile.
Jimena Sierralta
Affiliation:
Program in Physiology and Biophysics, Institute for Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile.
Manuel Kukuljan*
Affiliation:
Program in Physiology and Biophysics, Institute for Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile.
*
All correspondence to: Manuel Kukuljan. Program in Physiology and Biophysics, Institute for Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile. Tel: +56 2 978-6707. Fax: +56 2 777-6916. e-mail: kukuljan@neuro.med.uchile.cl
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Summary

Increases in cytosolic calcium concentrations regulate many cellular processes, including aspects of early development. Calcium release from intracellular stores and calcium entry through non-voltage-gated channels account for signalling in non-excitable cells, whereas voltage-gated calcium channels (CaV) are important in excitable cells. We report the expression of multiple transcripts of CaV, identified by its homology to other species, in the early embryo of the zebrafish, Danio rerio, at stages prior to the differentiation of excitable cells. CaV mRNAs and proteins were detected as early as the 2-cell stages, which indicate that they arise from both maternal and zygotic transcription. Exposure of embryos to pharmacological blockers of CaV does not perturb early development significantly, although late effects are appreciable. These results suggest that CaV may have a role in calcium homeostasis and control of cellular process during early embryonic development.

Keywords

Calcium channelsGastrulationZebrafish
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 131 - 135
Copyright
Copyright © Cambridge University Press 2009

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