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Expression and localization of urokinase-type plasminogen activator receptor in bovine cumulus–oocyte complexes

Published online by Cambridge University Press:  05 May 2015

Daniela C. García ,
Dora C. Miceli ,
Gabriela Rizo ,
Elina V. García ,
Pablo A. Valdecantos  and
Mariela Roldán-Olarte
Daniela C. García
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, San Miguel de Tucumán, Argentina.
Dora C. Miceli
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, San Miguel de Tucumán, Argentina. Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina.
Gabriela Rizo
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, San Miguel de Tucumán, Argentina. Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina.
Elina V. García
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, San Miguel de Tucumán, Argentina. Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina.
Pablo A. Valdecantos
Affiliation:
Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina.
Mariela Roldán-Olarte*
Affiliation:
Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina. Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, San Miguel de Tucumán, Argentina.
*
All correspondence to: Mariela Roldán-Olarte. Instituto de Biología ‘Dr. Francisco D. Barbieri’, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI – San Miguel de Tucumán, Argentina. Tel: +54 381 4247752 (ext. 7099). Fax: +54 381 4247752 (ext. 7004). e-mail: emroldanolarte@fbqf.unt.edu.ar
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Summary

Urokinase-type plasminogen activator (uPA) is a serine protease involved in extracellular matrix remodeling through plasmin generation. uPA usually binds to its receptor, uPAR, which is anchored to the plasma membrane through a glycosylphosphatidylinositol anchor. uPA/uPAR binding increases proteolytic activity in the neighborhood of the cells containing uPAR and activates intracellular signaling pathways involved in extracellular matrix remodeling, cell migration and proliferation. The aim of this work was to study the expression of uPA, uPAR and plasminogen activator inhibitor-1 (PAI-1) in immature and in vitro matured bovine cumulus–oocyte complexes (COCs). uPA is only expressed in the cumulus cells of immature and in vitro matured COCs, while uPAR and PAI-1 are expressed in both the cumulus cells and the immature and in vitro matured oocytes. In addition, uPAR protein was localized by confocal microscopy in the plasma membrane of oocytes and cumulus cells of immature COCs. Results from this research led us to hypothesize that the uPA/uPAR interaction could cause the local production of uPA-mediated plasmin over oocyte and cumulus cell surface; plasmin formation could also be regulated by PAI-1.

Keywords

BovineCumulus CellsOocytesuPAuPAR
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 230 - 235
Copyright
Copyright © Cambridge University Press 2015 

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