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Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells

  • Bartosz Kempisty (a1) (a2), Agnieszka Ziółkowska (a3), Sylwia Ciesiółka (a3), Hanna Piotrowska (a4), Paweł Antosik (a5), Dorota Bukowska (a5), Klaus P. Brüssow (a6), Michał Nowicki (a3) and Maciej Zabel (a7)...

Summary

Although the expression of estrogen and progesterone receptors within porcine ovary and cumulus–oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to real-time proliferation. In this study, a model of oocytes-separated cumulus cells was used to analyze the cell proliferation index and the expression PGR, PGRMC1 and of ERRγ and ERRβ/γ during 96-h cultivation in vitro using real-time quantitative PCR (qRT-PCR) and confocal microscopic observation. We found that PGR protein expression was increased at 0 h, compared with PGR protein expression after 96 h of culture (P < 0.001). The expression of PGRMC1, ERRγ and ERRβ/γ was unchanged. After using qRT-PCR we did not found statistical differences in expression of PGR, PGRMC1, ERRγ and ERRβ/γ during 96 h of cumulus cells in vitro culture (IVC). We supposed that the differential expression of the PGR protein at 0 h and after 96 h is related to a time-dependent down-regulation, which may activate a negative feedback. The distribution of PGR, PGRMC1 proteins may be linked with the translocation of receptors to the cytoplasm after the membrane binding of respective agonists and intra-cytoplasmic signal transduction. Furthermore, cumulus cells analyzed at 0 h were characterized by decreased proliferation index, whereas those after 96 h of culture revealed a significant increase of proliferation index, which may be associated with differentiation/luteinization of these cells during real-time proliferation.

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Corresponding author

All correspondence to: Bartosz Kempisty. Department of Histology and Embryology, Department of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60–781 Poznan, Poland. Tel: +48 61 8546419. Fax: +48 61 8546455. E-mail: etok@op.pl

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Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells

  • Bartosz Kempisty (a1) (a2), Agnieszka Ziółkowska (a3), Sylwia Ciesiółka (a3), Hanna Piotrowska (a4), Paweł Antosik (a5), Dorota Bukowska (a5), Klaus P. Brüssow (a6), Michał Nowicki (a3) and Maciej Zabel (a7)...

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