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Embryo co-culture with bovine amniotic membrane stem cells can enhance the cryo-survival of IVF-derived bovine blastocysts comparable with co-culture with bovine oviduct epithelial cells

Published online by Cambridge University Press:  20 October 2020

Shayan Nejat-Dehkordi ,
Ebrahim Ahmadi Open the ORCID record for Ebrahim Ahmadi [Opens in a new window] ,
Abolfazl Shirazi ,
Hassan Nazari  and
Naser Shams-Esfandabadi
Shayan Nejat-Dehkordi
Affiliation:
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Ebrahim Ahmadi
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
Abolfazl Shirazi*
Affiliation:
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Hassan Nazari
Affiliation:
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
Naser Shams-Esfandabadi
Affiliation:
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
*
Author for correspondence: Abolfazl Shirazi, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran and Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran. Tel: +98 21 2404144. Fax: +98 21 22404145. E-mail: shiraziabbas@yahoo.com
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Summary

Culture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then cultured up to the blastocyst stage in the following groups: (i) co-culture with bAMSCs, (ii) co-culture with BOECs, and (iii) cell-free culture (Con). Embryos that reached the blastocyst stage were vitrified and warmed, and their post-warming re-expansion, survival and hatching rates were evaluated after 72 h culture. Results showed that the cleavage, blastocyst, and 2 h post-warming re-expansion rates of embryos did not differ between groups. However, their survival rates in BOEC and bAMSC groups were significantly higher compared with the control (72.7, 75.6 and 37.5%, respectively, P < 0.05). In conclusion, our results showed that the cryo-survivability of IVF-derived bovine embryos could be improved through co-culturing with bAMSCs. Moreover, considering the possibility to provide multiple passages from bAMSCs compared with BOECs, due to their stemness properties and their ability to produce growth factors, the use of bAMSCs is a good alternative to BOECs in embryo co-culture systems.

Keywords

Adult stem cellsCulture systemEmbryo qualityFreezabilityVitrification
Type
Research Article
Information
Zygote , Volume 29 , Issue 2 , April 2021 , pp. 102 - 107
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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