Skip to main content Accessibility help

Efficiency of different incubation systems for the in vitro production of bovine embryos

  • Camila M. Cavalcanti (a1), Iana S. Campelo (a1), Mirelly M.A.S. Silva (a1), João V.S. Albuquerque (a1), Luciana M. Melo (a1) and Vicente J.F. Freitas (a1)...


This study aimed to compare the efficiency of different incubation systems for in vitro embryo production in bovine. Oocytes/embryos were cultured in three incubators: conventional – CONV, mini bench – MINI and portable – PORT. After in vitro maturation (IVM), oocytes were verified for maturation rate. The remaining structures were submitted to in vitro fertilization and culture to verify cleavage (day 2) and blastocyst (day 7) rates. Reactive oxygen species (ROS) were evaluated in post-IVM oocytes and embryos (days 2 and 7) using arbitrary fluorescence units (AFUs). No significant difference (P>0.05) was observed for maturation rate. The CONV system (74.0%) produced the highest cleavage rate (P<0.05) when compared with PORT (59.5%), but similar (P>0.05) to MINI (65.0%). The same pattern and differences were observed for blastocyst rate: CONV (33.3%), MINI (32.3%) and PORT (21.9%). ROS levels were not different (P>0.05) in post-IVM oocytes: CONV (35.6±4.5), MINI (29.4±4.0) and PORT (35.6±4.5). For day-2 embryos, ROS levels were higher (P<0.05) in MINI (44.2±3.1) in comparison with CONV (27.7±3.7) and PORT (33.3±3.2). No significant difference (P>0.05) was observed in blastocysts. In conclusion, although it produced high ROS levels at day 2 of culture, the MINI system was as efficient as the CONV system for blastocyst production. This option may be an interesting and economical for the in vitro embryo industry.


Corresponding author

*All correspondence to: Vicente José de Figueirêdo Freitas. Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil. Tel: +55 8531019861. E-mail:


Hide All
Ambrogi, M, Dall’Acqua, PC, Rocha-Frigoni, N, Leão, B Mingoti, GZ (2017) Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: effects on nuclear and cytoplasmic maturation and embryonic development in vitro . Reprod. Domest. Anim. 52, 409421.
Avery, B, Melsted, JK Greve, T (2000) A novel approach for in vitro production of bovine embryos: use of the oxoid atmosphere generating system. Theriogenology 54, 12591268.
Brackett, BG Oliphant, G (1975) Capacitation of rabbit spermatozoa in vitro . Biol. Reprod. 12, 260274.
Ciray, HN, Aksoy, T, Yaramanci, K, Karayaka, I Bahceci, M (2009) In vitro culture under physiologic oxygen concentration improves blastocyst yield and quality: a prospective randomized survey on sibling oocytes. Fertil. Steril. 91, 14591461.
Corrêa, GA, Rumpf, R, Mundim, T.C.D, Franco, MM Dode, MAN (2008) Oxygen tension during in vitro culture of bovine embryos: effect in production and expression of genes related to oxidative stress. Anim. Reprod. Sci. 104, 132142.
Diógenes, MN, Guimarães, A.L.S, Leme, LO, Maurício, MF Dode, MAN (2017) Effect of prematuration and maturation with fibroblast growth factor 10 (FGF10) on in vitro development of bovine oocytes. Theriogenology 102, 190198.
Fatehi, AN, Roelen, BA, Colenbrander, B, Schoevers, EJ, Gadella, BM, Beverst, MM van den Hurk, R (2005) Presence of cumulus cells during in vitro fertilization protects the bovine oocyte against oxidative stress and improves first cleavage but does not affect further development. Zygote 13, 177185.
Ferreira, CR, Saraiva, SA, Catharino, RR, Garcia, JS, Gozzo, FC, Sanvido, GB, Santos, LF, Lo Turco, EG, Pontes, JH, Basso, AC, Bertolla, RP, Sartori, R, Guardieiro, MM, Perecin, F, Meirelles, FV, Sangalli, JR Eberlin, MN (2010) Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 51, 12181227.
Fischer, B Bavister, BD (1993) Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J. Reprod. Fertil. 99, 673679.
Guemra, S, Santo, ES, Zanin, R, Monzani, PS, Sovernigo, TC, Ohashi, OM, Verde Leal, CL Adona, PR (2014) Effect of temporary meiosis block during prematuration of bovine cumulus–oocyte complexes on pregnancy rates in a commercial setting for in vitro embryo production. Theriogenology 81, 982987.
Guérin, P, El Mouatassim, S Menezo, Y (2001) Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175189.
Harvey, AJ, Kind, KL Thompson, JG (2002) REDOX regulation of early embryo development. Reproduction 123, 479486.
IETS (The International Embryo Transfer Society) (2015) Statistics of embryo collection and transfer in domestic farm animals.
Khazaei, M Aghaz, F (2017) Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes. Int. J. Fertil. Steril. 11, 6369.
Lane, M (2001) Mechanisms for managing cellular and homeostatic stress in vitro . Theriogenology 55, 225236.
Lee, M, Grazi, R Seifer, D (2013) Incorporation of the K-Minc incubator and media system into the IVF lab: the future of IVF. J. Clin. Embryol. 13, 2132.
Marinho, LSR, Untura, RM, Morotti, F, Moino, LL, Rigo, AG, Sanches, BV, Pontes, JHF Seneda, MM (2012) Large-scale programs for recipients of in vitro-produced embryos. Anim. Reprod. 9, 323328.
Martín-Romero, FJ, Miguel-Lasobras, EM, Domínguez-Arroyo, JA, González-Carrera, E Álvarez, IS (2008) Contribution of culture media to oxidative stress and its effect on human oocytes. Reprod. Biomed. Online 17, 652661.
Mingoti, GZ, Castro, VSDC, Méo, SC, Barretto, L.S.S Garcia, JM (2011) The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. In Vitro Cell. Dev. Biol. - Animal 47, 361367.
Oliveira, CS, Saraiva, NZ, Cruz, MH, Mazeti, B, Oliveira, LZ, Lopes, FL Garcia, JM (2013) HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction 145, 917.
Pereira, MM, Machado, MA, Costa, FQ, Serapiao, RV, Viana, JH Camargo, LS (2010) Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reprod. Fertil. Dev. 22, 10741082.
Pontes, JHF, Nonato-Junior, I, Sanches, BV, Ereno-Junior, JC, Uvo, S, Barreiros, TRR, Oliveira, JA, Hasler, JF Seneda, MM (2009) Comparison of embryo yield and pregnancy rate between in vivo and in vitro methods in the same Nelore (Bos indicus) donor cows. Theriogenology 71, 690697.
Pontes, JH, Melo-Sterza, FA, Basso, AC, Ferreira, CR, Sanches, BV, Rubin, KC Seneda, MM (2010) Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology 75, 16401646.
Rinaudo, PF, Giritharan, G, Talbi, S, Dobson, AT Schultz, RM (2006) Effects of oxygen tension on gene expression in preimplantation mouse embryos. Fertil. Steril. 86, 12521265.
Sugiyama, S, McGowan, M, Phillips, N, Kafi, M Young, M (2007) Effects of increased ambient temperature during IVM and/or IVF on the in vitro development of bovine zygotes. Reprod. Domest. Anim. 42, 271274.
Thompson, JGE, Simpson, AC, Pugh, PA, Donnelly, PE Tervit, HR (1990) Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. J. Reprod. Fertil. 89, 573578.
Wang, F, Tian, XZ, Zhang, L, He, CJ, Ji, PY, Li, Y, Tan, DX Liu, GS (2014) Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization. Fertil. Steril. 101, 577586.
Yang, HW, Hwang, KJ, Kwon, HC, Kim, HS, Choi, KW Oh, KS (1998) Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum. Reprod. 13, 9981002.
Yuan, YQ, Van Soom, A, Coopman, F.O.J, Mintiens, K, Boerjan, ML, Van Zeveren, A, de Kruif, A Peelman, LJ (2003) Influence of oxygen tension on apoptosis and hatching in bovine embryos cultured in vitro . Theriogenology 59, 15851596.


Efficiency of different incubation systems for the in vitro production of bovine embryos

  • Camila M. Cavalcanti (a1), Iana S. Campelo (a1), Mirelly M.A.S. Silva (a1), João V.S. Albuquerque (a1), Luciana M. Melo (a1) and Vicente J.F. Freitas (a1)...


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed