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The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status

Published online by Cambridge University Press:  14 December 2012

S.S.M. Faezah ,
F.M.Y Zuraina ,
J.H.F. Farah ,
O. Khairul ,
N.I. Hilwani ,
M.I. Iswadi ,
C.N. Fang ,
I. Zawawi ,
O.M. Abas  and
S.I. Fatimah
S.S.M. Faezah
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
F.M.Y Zuraina
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
J.H.F. Farah
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
O. Khairul
Affiliation:
Forensic Science Programme, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
N.I. Hilwani
Affiliation:
Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor D.E., Malaysia.
M.I. Iswadi
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
C.N. Fang
Affiliation:
Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
I. Zawawi
Affiliation:
Institut Bioteknologi Veterinar Kebangsaan, Bukit Dinding, 27000 Jerantut, Pahang, Malaysia.
O.M. Abas
Affiliation:
Agro-Biotechnology Institute, Malaysia, d/o MARDI Headquarters, 43400 Serdang, Selangor D.E., Malaysia.
S.I. Fatimah*
Affiliation:
Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
*
All correspondence to S.I. Fatimah. Department of Physiology, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Tel: +603 9289 7641. Fax: +603 2693 9687. E-mail: timi@medic.ukm.my
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Summary

Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

Keywords

CapacitationMagnetic-activated cell sorting systemThawingViability
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 378 - 386
Copyright
Copyright © Cambridge University Press 2012 

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