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Effects of low-density lipoproteins extracted from different avian yolks on boar spermatozoa quality following freezing–thawing

Published online by Cambridge University Press:  30 November 2012

Peng Wang ,
Yan-Feng Wang ,
Chun-Wei Wang ,
Shu-Hai Bu ,
Jian-Hong Hu ,
Qing-Wang Li ,
Wei-Jun Pang  and
Gong-She Yang
Peng Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Yan-Feng Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Chun-Wei Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Shu-Hai Bu
Affiliation:
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Jian-Hong Hu*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Qing-Wang Li*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Wei-Jun Pang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Gong-She Yang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
*
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164. e-mail: hjh19732008@126.com or liqingwangysu@yahoo.com.cn
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164. e-mail: hjh19732008@126.com or liqingwangysu@yahoo.com.cn
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Summary

Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. LDL extracted from the yolk of hen egg, duck egg, quail egg, pigeon egg or ostrich egg was added to the extender at the concentrations of 0.06, 0.07, 0.08, 0.09 and 0.1 g/ml, respectively, and their effects on frozen–thawed boar sperm quality were assessed. According to all measured parameters, the results showed that sperm motility, acrosome integrity and plasma membrane integrity were 43.20%, 52.57% and 48.13%, respectively, after being frozen–thawed with 0.09 g/ml LDL extracted from pigeon egg yolk. All these quality parameters were higher than that of other groups (P < 0.05). In conclusion, our results confirmed that LDL extracted from pigeon egg yolk had the best cryoprotective effects on frozen–thawed boar sperm among all of the groups supplemented with LDL from five kinds of avian egg in extender. The optimum concentration of LDL extracted from pigeon egg in boar semen freezing extender was 0.09 g/ml.

Keywords

Avian egg yolk LDLBoar semenCryopreservationSpermatozoa quality parameters
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 175 - 181
Copyright
Copyright © Cambridge University Press 2012 

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