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The effect of pentoxifylline and calcium ionophore treatment on sperm cell biology in oligoasthenoteratozoospermia samples

Published online by Cambridge University Press:  14 December 2022

Maryam Mahaldashtian Open the ORCID record for Maryam Mahaldashtian [Opens in a new window] ,
Mohammad Ali Khalili Open the ORCID record for Mohammad Ali Khalili [Opens in a new window] ,
Mahboubeh Vatanparast Open the ORCID record for Mahboubeh Vatanparast [Opens in a new window] ,
Fatemeh Anbari ,
Ali Nabi  and
Esmat Mangoli
Maryam Mahaldashtian
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Ali Khalili*
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mahboubeh Vatanparast
Affiliation:
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Fatemeh Anbari
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Ali Nabi
Affiliation:
Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Esmat Mangoli
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
*
Author for correspondence: Mohammad Ali Khalili. Professor of Embryology, Yazd Reproductive Sciences Institute, Iran. Tel: +983518247085. Fax: +983518247087. E-mail: Khalili59@hotmail.com
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Summary

The objective of this study was to assess the effects of pentoxifylline (PTX) and Ca2+ ionophore (CI) A12387 treatment on some biological characteristics of sperm cells in oligoasthenoteratozoospermia (OAT) patients. After processing, each sample was divided into four groups: 1, control; 2, exposed to 3.6 mM PTX; 3, exposed to 5 μm calcium ionophore (CI); and 4, exposed to both PTX and CI; 30 min at 37°C. Sperm motility was measured before and after preparation. Acrosome reaction (AR), status of sperm vacuoles, mitochondrial membrane potential (MMP) and DNA fragmentation were assessed using PSA-FITC staining, motile sperm organelle morphology examination (MSOME), JC-1 staining and sperm chromatin dispersion (CSD) test, respectively. Treatment with PTX and CI led to increased and decreased sperm motility, respectively (P < 0.05). Furthermore, vacuole status and rates of sperm DNA fragmentation were not significantly different among groups (P > 0.05). Moreover, the data showed that the rates of AR and disrupted MMP were significantly different between groups (P < 0.05). In conclusion, in vitro application of PTX not only did not have any adverse effects on sperm cell biology characteristics, but also can rectify the harmful effect of CI.

Keywords

Acrosome reactionCalcium ionophoreDNA fragmentationMitochondrial membrane potentialOligoasthenoteratozoospermiaPentoxifyllineVacuole
Type
Research Article
Information
Zygote , Volume 31 , Issue 1 , February 2023 , pp. 85 - 90
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Copyright
© The Author(s), 2022. Published by Cambridge University Press

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