Hostname: page-component-848d4c4894-ttngx Total loading time: 0 Render date: 2024-05-07T23:02:30.228Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro

Published online by Cambridge University Press:  16 September 2013

Mohammad Salehi ,
Yoko Kato  and
Yukio Tsunoda
Mohammad Salehi
Affiliation:
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Yoko Kato*
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.
Yukio Tsunoda
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.
*
All correspondence to: Yoko Kato. Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan. Tel: +81 742 43 5393. Fax: +81 742 43 5393. e-mail: yoko@nara.kindai.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on mouse somatic cell nuclear transfer remains unknown. In this study, we assessed the effects of various concentrations of melatonin (10−6 to 10−12 M) on the in vitro development of mouse somatic cell nuclear transfer embryos for 96 h. Embryos cultured without melatonin were used as control. There was no significant difference in cleavage rates between the groups supplemented with melatonin, dimethyl sulphoxide (DMSO) and the control. The rate of development to blastocyst stage was significantly higher in the group supplemented with 10−12 M melatonin compared with the control group (P < 0.05). Thus, our data demonstrated that adding melatonin to pre-implantation mouse nuclear-transferred embryos can accelerate blastocyst formation.

Keywords

MelatoninMouse somatic cell nuclear transferReactive oxygen species
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 213 - 217
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Choi, J., Park, S.M., Lee, E., Kim, J.H., Jeong, Y.I., Lee, J.Y., Park, S.W., Kim, H.S., Hossein, M.S., Jeong, Y.W., Kim, S., Hyun, S.H. & Hwang, W.S. (2008). Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryos. Mol. Reprod. Dev. 75, 1127–35.Google Scholar
Guerin, P., El Mouatassim, S. & Menezo, Y. (2001). Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175–89.Google Scholar
Halliwell, B. & Aruoma, O.I. (1991). DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett. 281, 9–19.CrossRefGoogle ScholarPubMed
Ishizuka, B., Kuribayashi, Y., Murai, K., Amemiya, A. & Itoh, M.T. (2000). The effect of melatonin on in vitro fertilization and embryo development in mice. J. Pineal Res. 28, 48–51.Google Scholar
Kishigami, S., Mizutani, E., Ohta, H., Hikichi, T., Thuan, N.V., Wakayama, S., Bui, H.T. & Wakayama, T. (2006a). Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340, 183–9.CrossRefGoogle ScholarPubMed
Kishigami, S., Van Thuan, N., Hikichi, T., Ohta, H., Wakayama, S., Mizutani, E. & Wakayama, T. (2006b). Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids. Dev. Biol. 289, 195–205.CrossRefGoogle ScholarPubMed
Korkmaz, A. & Reiter, R.J. (2008). Epigenetic regulation: a new research area for melatonin? J. Pineal Res. 44, 41–4.Google Scholar
Kowaltowski, A.J. & Vercesi, A.E. (1999). Mitochondrial damage induced by conditions of oxidative stress. Free Radic. Biol. Med. 26, 463–71.Google Scholar
Legge, M. & Sellens, M.H. (1991). Free radical scavengers ameliorate the 2-cell block in mouse embryo culture. Hum. Reprod. 6, 867–71.CrossRefGoogle ScholarPubMed
Mayo, J.C., Sainz, R.M., Antoli, I., Herrera, F., Martin, V. & Rodriguez, C. (2002). Melatonin regulation of antioxidant enzyme gene expression. Cell. Mol. Life Sci. 59, 1706–13.Google Scholar
McElhinny, A.S., Davis, F.C. & Warner, C.M. (1996). The effect of melatonin on cleavage rate of C57BL/6 and CBA/Ca preimplantation embryos cultured in vitro. J. Pineal Res. 21, 44–8.Google Scholar
Momparler, R.L. & Bovenzi, V. (2000). DNA methylation and cancer. J. Cell. Physiol. 183, 145–54.Google Scholar
Nakano, M., Kato, Y. & Tsunoda, Y. (2012). Effect of melatonin treatment on the developmental potential of parthenogenetic and somatic cell nuclear-transferred porcine oocytes in vitro. Zygote 20, 199–207.Google Scholar
Noda, Y., Matsumoto, H., Umaoka, Y., Tatsumi, K., Kishi, J. & Mori, T. (1991). Involvement of superoxide radicals in the mouse two-cell block. Mol. Reprod. Dev. 28, 356–60.Google Scholar
Papis, K., Poleszczuk, O., Wenta-Muchalska, E. & Modlinski, J.A. (2007). Melatonin effect on bovine embryo development in vitro in relation to oxygen concentration. J. Pineal Res. 43, 321–6.Google Scholar
Poeggeler, B., Reiter, R.J., Tan, D.X., Chen, L.D. & Manchester, L.C. (1993). Melatonin, hydroxyl radical-mediated oxidative damage, and aging: a hypothesis. J. Pineal Res. 14, 151–68.Google Scholar
Reiter, R J., Tan, D.X., Osuna, C. & Gitto, E. (2000). Actions of melatonin in the reduction of oxidative stress. A review. J. Biomed. Sci. 7, 444–58.Google Scholar
Rodriguez-Osorio, N., Kim, I.J., Wang, H., Kaya, A. & Memili, E. (2007). Melatonin increases cleavage rate of porcine preimplantation embryos in vitro. J. Pineal Res. 43, 283–8.Google Scholar
Rybouchkin, A., Kato, Y. & Tsunoda, Y. (2006). Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74, 1083–9.Google Scholar
Sharma, R., Ottenhof, T., Rzeczkowska, P.A. & Niles, L. P. (2008). Epigenetic targets for melatonin: induction of histone H3 hyperacetylation and gene expression in C17.2 neural stem cells. J. Pineal Res. 45, 277–84.Google Scholar
Tamura, H., Nakamura, Y., Korkmaz, A., Manchester, L.C., Tan, D.X., Sugino, N. & Reiter, R.J. (2009). Melatonin and the ovary: physiological and pathophysiological implications. Fertil. Steril. 92, 328–43.Google Scholar
Tian, X.Z., Wen, Q., Shi, J.M., Liang, W., Zeng, S.M., Tian, J.H., Zhou, G.B., Zhu, S.E. & Liu, G.S. (2010). Effects of melatonin on in vitro development of mouse two-cell embryos cultured in HTF medium. Endocr. Res. 35, 17–23.CrossRefGoogle ScholarPubMed
Tsuji, Y., Kato, Y. & Tsunoda, Y. (2009). The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2′-deoxycytidine. Zygote 17, 109–15.Google Scholar