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Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality

Published online by Cambridge University Press:  02 May 2012

Rafael Rossetto ,
Márcia Viviane Alves Saraiva ,
Regiane Rodrigues dos Santos ,
Cleidson Manoel Gomes da Silva ,
Luciana Rocha Faustino ,
Roberta Nogueira Chaves ,
Ivina Rocha Brito ,
Giovanna Quintino Rodrigues ,
Isadora Machado Teixeira Lima  and
Mariana Aragão Matos Donato
...Show all authors
Rafael Rossetto*
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, Brasil.
Márcia Viviane Alves Saraiva
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Regiane Rodrigues dos Santos
Affiliation:
Laboratory of Biology and Medicine of Wild Mammals from Amazonia, Federal University of Pará, Belém, PA, Brazil.
Cleidson Manoel Gomes da Silva
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Luciana Rocha Faustino
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Roberta Nogueira Chaves
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Ivina Rocha Brito
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Giovanna Quintino Rodrigues
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Isadora Machado Teixeira Lima
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Mariana Aragão Matos Donato
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
Christina Alves Peixoto
Affiliation:
Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil.
José Ricardo de Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
*
All correspondence to: Rafael Rossetto. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, Brasil. CEP: 60740-000. Tel: +55 85 3101.9852. Fax: +55 85 3101 9840. e-mail: rafael.rossetto@hotmail.com
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Summary

This study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.

Keywords

AntrumIVCPre-antral ovarian follicleUltrastructureViability
Type
Research Article
Information
Zygote , Volume 21 , Issue 2 , May 2013 , pp. 125 - 128
Copyright
Copyright © Cambridge University Press 2012

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